322 research outputs found
Age-Related Mushroom Body Expansion in Male Sweat Bees and Bumble Bees
A well-documented phenomenon among social insects is that brain changes occur prior to or at the onset of certain experiences, potentially serving to prime the brain for specific tasks. This insight comes almost exclusively from studies considering developmental maturation in females. As a result, it is unclear whether age-related brain plasticity is consistent across sexes, and to what extent developmental patterns differ. Using confocal microscopy and volumetric analyses, we investigated age-related brain changes coinciding with sexual maturation in the males of the facultatively eusocial sweat bee, Megalopta genalis, and the obligately eusocial bumble bee, Bombus impatiens. We compared volumetric measurements between newly eclosed and reproductively mature males kept isolated in the lab. We found expansion of the mushroom bodiesâbrain regions associated with learning and memoryâwith maturation, which were consistent across both species. This age-related plasticity may, therefore, play a functionally-relevant role in preparing male bees for mating, and suggests that developmentally-driven neural restructuring can occur in males, even in species where it is absent in females
A conspicuous tangential alignment of galaxies in a STIS Parallel Shear Survey field: A new dark-lens candidate ?
We report the serendipituous discovery of a conspicuous alignment of galaxies
in a field obtained through the STIS Parallel Shear Survey. This project
collects randomly distributed 50'' x 50'' fields to investigate the cosmic
shear effect on this scale. Analyzing the parallel observations having the
Seyfert galaxy NGC625 as primary target, we recognized over the whole field of
view a strong apparent tangential alignment of galaxy ellipticities towards the
image center. The field shows several arclet-like features typical for images
of massive galaxy clusters, but no obvious over-density of bright foreground
galaxies. We also find a multiple image candidate. On the basis of the possible
strong and weak lensing effect within the data, we discuss whether this could
be compatible with a massive halo with no clear optical counterpart.Comment: 6 pages, 2 figures, submitted to A&
Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A
AbstractAdipose triglyceride lipase (ATGL) is required for efficient mobilization of triglyceride (TG) stores in adipose tissue and non-adipose tissues. Therefore, ATGL strongly determines the availability of fatty acids for metabolic reactions. ATGL activity is regulated by a complex network of lipolytic and anti-lipolytic hormones. These signals control enzyme expression and the interaction of ATGL with the regulatory proteins CGI-58 and G0S2. Up to date, it was unknown whether ATGL activity is also controlled by lipid intermediates generated during lipolysis. Here we show that ATGL activity is inhibited by long-chain acyl-CoAs in a non-competitive manner, similar as previously shown for hormone-sensitive lipase (HSL), the rate-limiting enzyme for diglyceride breakdown in adipose tissue. ATGL activity is only marginally inhibited by medium-chain acyl-CoAs, diglycerides, monoglycerides, and free fatty acids. Immunoprecipitation assays revealed that acyl-CoAs do not disrupt the proteinâprotein interaction of ATGL and its co-activator CGI-58. Furthermore, inhibition of ATGL is independent of the presence of CGI-58 and occurs directly at the N-terminal patatin-like phospholipase domain of the enzyme. In conclusion, our results suggest that inhibition of the major lipolytic enzymes ATGL and HSL by long-chain acyl-CoAs could represent an effective feedback mechanism controlling lipolysis and protecting cells from lipotoxic concentrations of fatty acids and fatty acid-derived lipid metabolites
Qualitative evaluation of a preventive intervention for the offspring of parents with a history of depression
Background:
Meta-analyses of randomised controlled trials suggest that psychological interventions to reduce childrenâs risk of depression are effective. Nevertheless, these effects are modest and diminish over time. The Medical Research Council recommends a mixed-methods approach to the evaluation of complex interventions. By gaining a more thorough understanding of participantsâ perspectives, qualitative evaluations of preventive interventions could improve their efficacy, longevity and transfer into clinical practice.
Methods:
18 parents and 22 children who had received a 12-session family- and group-based cognitivebehavioural intervention to prevent youth depression as part of a randomised controlled trial took part in semistructured interviews or a focus group about aspects which had been perceived as helpful, elements they were still using after the intervention had ended, and suggestions they had for improving the intervention.
Results:
The chance to openly share and discuss their experiences of depression within and between families was considered helpful by both children and parents. Children benefitted the most from learning coping strategies for dealing with stress and many still used them in everyday life. Parents profited mostly from increasing positive family time, but noted that maintaining new routines after the end of the intervention proved difficult. Participants were generally content with the intervention but commented on how tiring and time consuming it was.
Conclusions:
Managing parentsâ expectations of family-based interventions in terms of their own mental health needs (versus those of their children) and leaving more room for open discussions may result in interventions which are more appealing to participating families. Increasing intervals between sessions may be one means of improving the longevity of interventions.
Trial registration: The original RCT this evaluation is a part of was registered under NCT02115880
Formation of massive seed black holes via collisions and accretion
Models aiming to explain the formation of massive black hole seeds, and in particular the direct collapse scenario, face substantial difficulties. These are rooted in rather ad hoc and fine-tuned initial conditions, such as the simultaneous requirements of extremely low metallicities and strong radiation backgrounds. Here, we explore a modification of such scenarios where a massive primordial star cluster is initially produced. Subsequent stellar collisions give rise to the formation of massive (104-105 Mâ) objects. Our calculations demonstrate that the interplay among stellar dynamics, gas accretion, and protostellar evolution is particularly relevant. Gas accretion on to the protostars enhances their radii, resulting in an enhanced collisional cross-section. We show that the fraction of collisions can increase from 0.1 to 1 per cent of the initial population to about 10 per cent when compared to gas-free models or models of protostellar clusters in the local Universe. We conclude that very massive objects can form in spite of initial fragmentation, making the first massive protostellar clusters viable candidate birth places for observed supermassive black holes
Skin Barrier Development Depends on CGI-58 Protein Expression during Late-Stage Keratinocyte Differentiation
Adipose triglyceride lipase (ATGL) and its coactivator comparative gene identification-58 (CGI-58) are limiting in cellular triglyceride catabolism. Although ATGL deficiency is compatible with normal skin development, mice globally lacking CGI-58 die postnatally and exhibit a severe epidermal permeability barrier defect, which may originate from epidermal and/or peripheral changes in lipid and energy metabolism. Here, we show that epidermis-specific disruption of CGI-58 is sufficient to provoke a defect in the formation of a functional corneocyte lipid envelope linked to impaired Ï-O-acylceramide synthesis. As a result, epidermis-specific CGI-58-deficient mice show severe skin dysfunction, arguing for a tissue autonomous cause of disease development. Defective skin permeability barrier formation in global CGI-58-deficient mice could be reversed via transgenic restoration of CGI-58 expression in differentiated but not basal keratinocytes suggesting that CGI-58 is essential for lipid metabolism in suprabasal epidermal layers. The compatibility of ATGL deficiency with normal epidermal function indicated that CGI-58 may stimulate an epidermal triglyceride lipase beyond ATGL required for the adequate provision of fatty acids as a substrate for Ï-O-acylceramide synthesis. Pharmacological inhibition of ATGL enzyme activity similarly reduced triglyceride-hydrolytic activities in wild-type and CGI-58 overexpressing epidermis implicating that CGI-58 participates in Ï-O-acylceramide biogenesis independent of its role as a coactivator of epidermal triglyceride catabolism
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