78 research outputs found
Performance Verification of the FlashCam Prototype Camera for the Cherenkov Telescope Array
The Cherenkov Telescope Array (CTA) is a future gamma-ray observatory that is
planned to significantly improve upon the sensitivity and precision of the
current generation of Cherenkov telescopes. The observatory will consist of
several dozens of telescopes with different sizes and equipped with different
types of cameras. Of these, the FlashCam camera system is the first to
implement a fully digital signal processing chain which allows for a traceable,
configurable trigger scheme and flexible signal reconstruction. As of autumn
2016, a prototype FlashCam camera for the medium-sized telescopes of CTA nears
completion. First results of the ongoing system tests demonstrate that the
signal chain and the readout system surpass CTA requirements. The stability of
the system is shown using long-term temperature cycling.Comment: 5 pages, 13 figures, Proceedings of the 9th International Workshop on
Ring Imaging Cherenkov Detectors (RICH 2016), Lake Bled, Sloveni
FlashCam: a fully-digital camera for the medium-sized telescopes of the Cherenkov Telescope Array
The FlashCam group is currently preparing photomultiplier-tube based cameras
proposed for the medium-sized telescopes (MST) of the Cherenkov Telescope Array
(CTA). The cameras are designed around the FlashCam readout concept which is
the first fully-digital readout system for Cherenkov cameras, based on
commercial FADCs and FPGAs as key components for the front-end electronics
modules and a high performance camera server as back-end. This contribution
describes the progress of the full-scale FlashCam camera prototype currently
under construction, as well as performance results also obtained with earlier
demonstrator setups. Plans towards the production and implementation of
FlashCams on site are also briefly presented.Comment: 8 pages, 6 figures. In Proceedings of the 34th International Cosmic
Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions
at arXiv:1508.0589
Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale
Crystallization conditions of an intact monoclonal IgG4 (immunoglobulin G, subclass 4) antibody were established in vapor diffusion mode by sparse matrix screening and subsequent optimization. The procedure was transferred to microbatch conditions and a phase diagram was built showing surprisingly low solubility of the antibody at equilibrium. With up-scaling to process scale in mind, purification efficiency of the crystallization step was investigated. Added model protein contaminants were excluded from the crystals to more than 95%. No measurable loss of Fc-binding activity was observed in the crystallized and redissolved antibody. Conditions could be adapted to crystallize the antibody directly from concentrated and diafiltrated cell culture supernatant, showing purification efficiency similar to that of Protein A chromatography. We conclude that crystallization has the potential to be included in downstream processing as a low-cost purification or formulation step
Tolerance of allogromiid Foraminifera to severely elevated carbon dioxide concentrations : implications to future ecosystem functioning and paleoceanographic interpretations
Author Posting. © Elsevier B.V., 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Global and Planetary Change 65 (2009): 107-114, doi:10.1016/j.gloplacha.2008.10.013.Increases in the partial pressure of carbon dioxide (pCO2) in the atmosphere will
significantly affect a wide variety of terrestrial fauna and flora. Because of tight atmospheric-oceanic
coupling, shallow-water marine species are also expected to be affected by increases in
atmospheric carbon dioxide concentrations. One proposed way to slow increases in atmospheric
pCO2 is to sequester CO2 in the deep sea. Thus, over the next few centuries marine species will
be exposed to changing seawater chemistry caused by ocean-atmospheric exchange and/or deep-ocean
sequestration. This initial case study on one allogromiid foraminiferal species
(Allogromia laticollaris) was conducted to begin to ascertain the effect of elevated pCO2 on
benthic Foraminifera, which are a major meiofaunal constituent of shallow- and deep-water
marine communities. Cultures of this thecate foraminiferan protist were used for 10-14-day
experiments. Experimental treatments were executed in an incubator that controlled CO2 (15
000; 30 000; 60 000; 90 000; 200 000 ppm), temperature and humidity; atmospheric controls
(i.e., ~375 ppm CO2) were executed simultaneously. Although the experimental elevated pCO2
values are far above foreseeable surface water pCO2, they were selected to represent the
spectrum of conditions expected for the benthos if deep-sea CO2 sequestration becomes a reality.
Survival was assessed in two independent ways: pseudopodial presence/absence and
measurement of adenosine triphosphate (ATP), which is an indicator of cellular energy.
Substantial proportions of A. laticollaris populations survived 200 000 ppm CO2 although the
mean of the median [ATP] of survivors was statistically lower for this treatment than for that of
atmospheric control specimens. After individuals that had been incubated in 200 000 ppm CO2
for 12 days were transferred to atmospheric conditions for ~24 hours, the [ATP] of live
specimens (survivors) approximated those of the comparable atmospheric control treatment.
Incubation in 200 000 ppm CO2 also resulted in reproduction by some individuals. Results
suggest that certain Foraminifera are able to tolerate deep-sea CO2 sequestration and perhaps
thrive as a result of elevated pCO2 that is predicted for the next few centuries, in a high-pCO2
world. Thus, allogromiid foraminiferal âbloomsâ may result from climate change. Furthermore,
because allogromiids consume a variety of prey, it is likely that they will be major players in
ecosystem dynamics of future coastal sedimentary environments.This work was funded by US Department of Energy grant # DE-FG02-03ER63696 (to
J. Kennett and J. Bernhard), NSF OCE-0725966, and the WHOI Summer Student Fellow
Program, which is funded by NSF Research Experience for Undergraduates Program grant
#OCE-0139423
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
Background: Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations and adaptations which occur in their natural surroundings, but also in industrial processes. Notably, the majority of such transcriptome analyses were performed using non-engineered strains. In this comparative study, the gene expression profiles of S. cerevisiae and P. pastoris, a Crabtree positive and Crabtree negative yeast, respectively, were analyzed for three different oxygenation conditions (normoxic, oxygen-limited and hypoxic) under recombinant protein producing conditions in chemostat cultivations. Results: The major differences in the transcriptomes of S. cerevisiae and P. pastoris were observed between hypoxic and normoxic conditions, where the availability of oxygen strongly affected ergosterol biosynthesis, central carbon metabolism and stress responses, particularly the unfolded protein response. Steady state conditions under low oxygen set-points seemed to perturb the transcriptome of S. cerevisiae to a much lesser extent than the one of P. pastoris, reflecting the major tolerance of the baker's yeast towards oxygen limitation, and a higher fermentative capacity. Further important differences were related to Fab production, which was not significantly affected by oxygen availability in S. cerevisiae, while a clear productivity increase had been previously reported for hypoxically grown P. pastoris. Conclusions: The effect of three different levels of oxygen availability on the physiology of P. pastoris and S. cerevisiae revealed a very distinct remodelling of the transcriptional program, leading to novel insights into the different adaptive responses of Crabtree negative and positive yeasts to oxygen availability. Moreover, the application of such comparative genomic studies to recombinant hosts grown in different environments might lead to the identification of key factors for efficient protein production
«La relation de limitation et dâexception dans le français dâaujourdâhui : exceptĂ©, sauf et hormis comme pivots dâune relation algĂ©brique »
Lâanalyse des emplois prĂ©positionnels et des emplois conjonctifs dâ âexceptĂ©â, de âsaufâ et dâ âhormisâ permet dâenvisager les trois prĂ©positions/conjonctions comme le pivot dâun binĂŽme, comme la plaque tournante dâune structure bipolaire. PlacĂ©es au milieu du binĂŽme, ces prĂ©positions sont forcĂ©es par leur sĂ©mantisme originaire dĂ»ment mĂ©taphorisĂ© de jouer le rĂŽle de marqueurs dâinconsĂ©quence systĂ©matique entre lâĂ©lĂ©ment se trouvant Ă leur gauche et celui qui se trouve Ă leur droite. Lâopposition qui surgit entre les deux Ă©lĂ©ments nâest donc pas une incompatibilitĂ© naturelle, intrinsĂšque, mais extrinsĂšque, induite. Dans la plupart des cas (emplois limitatifs), cette opposition prend la forme dâun rapport entre une « classe » et le « membre (soustrait) de la classe », ou bien entre un « tout » et une « partie » ; dans dâautres (emplois exceptifs), cette opposition se manifeste au contraire comme une attaque de front portĂ©e par un « tout » Ă un autre « tout ». De plus, lâinconsĂ©quence induite mise en place par la prĂ©position/conjonction paraĂźt, en principe, tout Ă fait insurmontable. Dans lâassertion « les Ă©cureuils vivent partout, sauf en Australie » (que lâon peut expliciter par « Les Ă©cureuils vivent partout, sauf [quâils ne vivent pas] en Australie »), la prĂ©position semble en effet capable dâimpliquer le prĂ©dicat principal avec signe inverti, et de bĂątir sur une telle implication une sorte de sous Ă©noncĂ© qui, Ă la rigueur, est totalement inconsĂ©quent avec celui qui le prĂ©cĂšde (si « les Ă©cureuils ne vivent pas en Australie », le fait quâils « vivent partout » est faux). NĂ©anmoins, lâanalyse montre quâalors que certaines de ces oppositions peuvent enfin ĂȘtre dĂ©passĂ©es, dâautres ne le peuvent pas. Câest, respectivement, le cas des relations limitatives et des relations exceptives. La relation limitative, impliquant le rapport « tout » - « partie », permet de rĂ©soudre le conflit dans les termes dâune somme algĂ©brique entre deux sous Ă©noncĂ©s pourvus de diffĂ©rent poids informatif et de signe contraire. Les valeurs numĂ©riques des termes de la somme Ă©tant dĂ©sĂ©quilibrĂ©es, le rĂ©sultat est toujours autre que zĂ©ro. La relation exceptive, au contraire, qui nâimplique pas le rapport « tout » - « partie », nâest pas capable de rĂ©soudre le conflit entre deux sous Ă©noncĂ©s pourvus du mĂȘme poids informatif et en mĂȘme temps de signe contraire : les valeurs numĂ©riques des termes de la somme Ă©tant symĂ©triques et Ă©gales, le rĂ©sultat sera toujours Ă©quivalent Ă zĂ©ro
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