Benthic and Pelagic Pathways of Methylmercury Bioaccumulation in Estuarine Food Webs of the Northeast United States

Methylmercury (MeHg) is a contaminant of global concern that bioaccumulates and bioamagnifies in marine food webs. Lower trophic level fauna are important conduits of MeHg from sediment and water to estuarine and coastal fish harvested for human consumption. However, the sources and pathways of MeHg to these coastal fisheries are poorly known particularly the potential for transfer of MeHg from the sediment to biotic compartments. Across a broad gradient of human land impacts, we analyzed MeHg concentrations in food webs at ten estuarine sites in the Northeast US (from the Hackensack Meadowlands, NJ to the Gulf of Maine). MeHg concentrations in water column particulate material, but not in sediments, were predictive of MeHg concentrations in fish (killifish and Atlantic silversides). Moreover, MeHg concentrations were higher in pelagic fauna than in benthic-feeding fauna suggesting that MeHg delivery to the water column from methylation sites from within or outside of the estuary may be an important driver of MeHg bioaccumulation in estuarine pelagic food webs. In contrast, bulk sediment MeHg concentrations were only predictive of concentrations of MeHg in the infaunal worms. Our results across a broad gradient of sites demonstrate that the pathways of MeHg to lower trophic level estuarine organisms are distinctly different between benthic deposit feeders and forage fish. Thus, even in systems with contaminated sediments, transfer of MeHg into estuarine food webs maybe driven more by the efficiency of processes that determine MeHg input and bioavailability in the water column

Good covers are algorithmically unrecognizable

A good cover in R^d is a collection of open contractible sets in R^d such that the intersection of any subcollection is either contractible or empty. Motivated by an analogy with convex sets, intersection patterns of good covers were studied intensively. Our main result is that intersection patterns of good covers are algorithmically unrecognizable. More precisely, the intersection pattern of a good cover can be stored in a simplicial complex called nerve which records which subfamilies of the good cover intersect. A simplicial complex is topologically d-representable if it is isomorphic to the nerve of a good cover in R^d. We prove that it is algorithmically undecidable whether a given simplicial complex is topologically d-representable for any fixed d \geq 5. The result remains also valid if we replace good covers with acyclic covers or with covers by open d-balls. As an auxiliary result we prove that if a simplicial complex is PL embeddable into R^d, then it is topologically d-representable. We also supply this result with showing that if a "sufficiently fine" subdivision of a k-dimensional complex is d-representable and k \leq (2d-3)/3, then the complex is PL embeddable into R^d.Comment: 22 pages, 5 figures; result extended also to acyclic covers in version

Nuclear Repulsion Enables Division Autonomy in a Single Cytoplasm

SummaryBackgroundCurrent models of cell-cycle control, based on classic studies of fused cells, predict that nuclei in a shared cytoplasm respond to the same CDK activities to undergo synchronous cycling. However, synchrony is rarely observed in naturally occurring syncytia, such as the multinucleate fungus Ashbya gossypii. In this system, nuclei divide asynchronously, raising the question of how nuclear timing differences are maintained despite sharing a common milieu.ResultsWe observe that neighboring nuclei are highly variable in division-cycle duration and that neighbors repel one another to space apart and demarcate their own cytoplasmic territories. The size of these territories increases as a nucleus approaches mitosis and can influence cycling rates. This nonrandom nuclear spacing is regulated by microtubules and is required for nuclear asynchrony, as nuclei that transiently come in very close proximity will partially synchronize. Sister nuclei born of the same mitosis are generally not persistent neighbors over their lifetimes yet remarkably retain similar division cycle times. This indicates that nuclei carry a memory of their birth state that influences their division timing and supports that nuclei subdivide a common cytosol into functionally distinct yet mobile compartments.ConclusionsThese findings support that nuclei use cytoplasmic microtubules to establish “cells within cells.” Individual compartments appear to push against one another to compete for cytoplasmic territory and insulate the division cycle. This provides a mechanism by which syncytial nuclei can spatially organize cell-cycle signaling and suggests size control can act in a system without physical boundaries

A coupled terrestrial and aquatic biogeophysical model of the Upper Merrimack River watershed, New Hampshire, to inform ecosystem services evaluation and management under climate and land-cover change

Accurate quantification of ecosystem services (ES) at regional scales is increasingly important for making informed decisions in the face of environmental change. We linked terrestrial and aquatic ecosystem process models to simulate the spatial and temporal distribution of hydrological and water quality characteristics related to ecosystem services. The linked model integrates two existing models (a forest ecosystem model and a river network model) to establish consistent responses to changing drivers across climate, terrestrial, and aquatic domains. The linked model is spatially distributed, accounts for terrestrial–aquatic and upstream–downstream linkages, and operates on a daily time-step, all characteristics needed to understand regional responses. The model was applied to the diverse landscapes of the Upper Merrimack River watershed, New Hampshire, USA. Potential changes in future environmental functions were evaluated using statistically downscaled global climate model simulations (both a high and low emission scenario) coupled with scenarios of changing land cover (centralized vs. dispersed land development) for the time period of 1980–2099. Projections of climate, land cover, and water quality were translated into a suite of environmental indicators that represent conditions relevant to important ecosystem services and were designed to be readily understood by the public. Model projections show that climate will have a greater influence on future aquatic ecosystem services (flooding, drinking water, fish habitat, and nitrogen export) than plausible changes in land cover. Minimal changes in aquatic environmental indicators are predicted through 2050, after which the high emissions scenarios show intensifying impacts. The spatially distributed modeling approach indicates that heavily populated portions of the watershed will show the strongest responses. Management of land cover could attenuate some of the changes associated with climate change and should be considered in future planning for the region

Violations of locality and free choice are equivalent resources in Bell experiments

Bell inequalities rest on three fundamental assumptions: realism, locality, and free choice, which lead to nontrivial constraints on correlations in very simple experiments. If we retain realism, then violation of the inequalities implies that at least one of the remaining two assumptions must fail, which can have profound consequences for the causal explanation of the experiment. We investigate the extent to which a given assumption needs to be relaxed for the other to hold at all costs, based on the observation that a violation need not occur on every experimental trial, even when describing correlations violating Bell inequalities. How often this needs to be the case determines the degree of, respectively, locality or free choice in the observed experimental behavior. Despite their disparate character, we show that both assumptions are equally costly. Namely, the resources required to explain the experimental statistics (measured by the frequency of causal interventions of either sort) are exactly the same. Furthermore, we compute such defined measures of locality and free choice for any nonsignaling statistics in a Bell experiment with binary settings, showing that it is directly related to the amount of violation of the so-called Clauser–Horne–Shimony–Holt inequalities. This result is theory independent as it refers directly to the experimental statistics. Additionally, we show how the local fraction results for quantum-mechanical frameworks with infinite number of settings translate into analogous statements for the measure of free choice we introduce. Thus, concerning statistics, causal explanations resorting to either locality or free choice violations are fully interchangeable

How large are the level sets of the Takagi function?

Let T be Takagi's continuous but nowhere-differentiable function. This paper considers the size of the level sets of T both from a probabilistic point of view and from the perspective of Baire category. We first give more elementary proofs of three recently published results. The first, due to Z. Buczolich, states that almost all level sets (with respect to Lebesgue measure on the range of T) are finite. The second, due to J. Lagarias and Z. Maddock, states that the average number of points in a level set is infinite. The third result, also due to Lagarias and Maddock, states that the average number of local level sets contained in a level set is 3/2. In the second part of the paper it is shown that, in contrast to the above results, the set of ordinates y with uncountably infinite level sets is residual, and a fairly explicit description of this set is given. The paper also gives a negative answer to a question of Lagarias and Maddock by showing that most level sets (in the sense of Baire category) contain infinitely many local level sets, and that a continuum of level sets even contain uncountably many local level sets. Finally, several of the main results are extended to a version of T with arbitrary signs in the summands.Comment: Added a new Section 5 with generalization of the main results; some new and corrected proofs of the old material; 29 pages, 3 figure

Bounding Helly numbers via Betti numbers

We show that very weak topological assumptions are enough to ensure the existence of a Helly-type theorem. More precisely, we show that for any non-negative integers $b$ and $d$ there exists an integer $h(b,d)$ such that the following holds. If $\mathcal F$ is a finite family of subsets of $\mathbb R^d$ such that $\tilde\beta_i\left(\bigcap\mathcal G\right) \le b$ for any $\mathcal G \subsetneq \mathcal F$ and every $0 \le i \le \lceil d/2 \rceil-1$ then $\mathcal F$ has Helly number at most $h(b,d)$. Here $\tilde\beta_i$ denotes the reduced $\mathbb Z_2$-Betti numbers (with singular homology). These topological conditions are sharp: not controlling any of these $\lceil d/2 \rceil$ first Betti numbers allow for families with unbounded Helly number. Our proofs combine homological non-embeddability results with a Ramsey-based approach to build, given an arbitrary simplicial complex $K$, some well-behaved chain map $C_*(K) \to C_*(\mathbb R^d)$.Comment: 29 pages, 8 figure

Deliberative multiattribute valuation of ecosystem services across a range of regional land-use, socioeconomic, and climate scenarios for the upper Merrimack River watershed, New Hampshire, USA

We evaluate the relative desirability of alternative futures for the upper Merrimack River watershed in New Hampshire, USA based on the value of ecosystem services at the end of the 21st century as gauged by its present-day inhabitants. This evaluation is accomplished by integrating land-use and socioeconomic scenarios, downscaled climate projections, biogeophysical simulation models, and the results of a citizen-stakeholder deliberative multicriteria evaluation. We find that although there are some trade-offs between alternative plausible futures, for the most part, it can be expected that future inhabitants of the watershed will be most satisfied if land-use planning in the intervening years prioritizes water supply and flood protection as well as maintenance of existing farmland and forest cover. With respect to climate change, it is expected that future watershed inhabitants will be more negatively affected by the projected loss of snow cover than the anticipated increase in hot summer days. More important than the specific results for the upper Merrimack River watershed, this integrative assessment demonstrates the complex yet ultimately informative potential to link stakeholder engagement with scenario generation, ecosystem models, and multiattribute evaluation for informing regional-scale planning and decision making