Correlation of cGAS, STING, INF-α and INF-β gene expression with Zika virus kinetics in primary culture of microglia and neurons from BALB/c mice

Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, BrasilPattern recognition receptors participate in the innate immune response. Among PRRs, the cGAS/STING pathway is known to detect cytosolic DNA and cyclic dinucleotides, but it’s also important in RNA virus infection. We aimed to evaluate the gene expression of some important genes of cGAS/STING pathway and to correlate this expression with Zika virus kinetics in mice microglia and neurons. Cells were infected by MOI = 1.0. Indirect immunofluorescence, plaque titration of supernatant, extraction, and quantification of total intracellular RNA, RT-qPCR and Western blotting were performed. Plaque titration profile in microglia and neurons was similar, including higher titers of plaque forming units at 24, 48, 72 and 96 hpi, respectively. ZIKV kinetics evaluated by RT-qPCR was similar in both cells, with highest viral titers at 48, 72, 24 and 96 hpi, respectively. Expression profile of cGAS, STING, INF-α and INF-β was quite different between the cells, including gene suppression, as observed for cGAS in neurons. Our results showed a differentiated expression profile of cGAS/STING pathway genes in mice microglia and neurons, which can be explained by the different mechanisms that ZIKV uses to bypass the immune response of these cells. Furthermore, each cell type responds differently to combat the viral infection

Oral manifestations of Wolf-Hirschhorn syndrome: genotype-phenotype correlation analysis

Background: Wolf-Hirschhorn syndrome (WHS) is a rare disease caused by deletion in the distal moiety of the short arm of chromosome 4. The objectives of this study were to report the most representative oral findings of WHS, relate them with other clinical characteristics of the disease, and establish possible phenotype-genotype correlation. Methods: The study was conducted at 6 reference centers distributed throughout Spain during 2018–2019. The study group consisted of 31 patients with WHS who underwent a standardized oral examination. Due to behavioral reasons, imaging studies were performed on only 11 of the children 6 years of age or older. All participants had previously undergone a specific medical examination for WHS, during which anatomical, functional, epilepsy-related, and genetic variables were recorded. Results: The most prevalent oral manifestations were delayed tooth eruption (74.1%), bruxism (64.5%), dental agenesis (63.6%), micrognathia (60.0%), oligodontia (45.5%), and downturned corners of the mouth (32.3%). We detected strong correlation between psychomotor delay and oligodontia (p = 0.008; Cramér’s V coefficient, 0.75). The size of the deletion was correlated in a statistically significant manner with the presence of oligodontia (p = 0.009 ; point-biserial correlation coefficient, 0.75). Conclusion: Certain oral manifestations prevalent in WHS can form part of the syndrome’s phenotypic variability. A number of the characteristics of WHS, such as psychomotor delay and epilepsy, are correlated with oral findings such as oligodontia and bruxism. Although most genotype-phenotype correlations are currently unknown, most of them seem to be associated with larger deletions, suggesting that some oral-facial candidate genes might be outside the critical WHS region, indicating that WHS is a contiguous gene syndrome

The Role of Climate, Marine Influence and Sedimentation Rates in Late-Holocene Estuarine Evolution (SW Portugal)

This is the Accepted Manuscript of the published document. This version of the article has been updated to include the author's revisions after peer review, prior to typesetting for the journal. The Final Published PDF is available online at: https://doi.org/10.1177/0959683618824768[Abstract] Estuaries are sensitive to changes in global to regional sea level, to climate-driven variation in rainfall and to fluvial discharge. In this study, we use source and environmentally sensitive proxies together with radiocarbon dating to examine a 7-m-thick sedimentary record from the Sado estuary accumulated throughout the last 3.6 kyr. The lithofacies, geochemistry and diatom assemblages in the sediments accumulated between 3570 and 3240 cal. BP indicate a mixture between terrestrial and marine sources. The relative contribution of each source varied through time as sedimentation progressed in a low intertidal to high subtidal and low-energy accreting tidal flat. The sedimentation proceeded under a general pattern of drier and higher aridity conditions, punctuated by century-long changes of the rainfall regime that mirror an increase in storminess that affected SW Portugal and Europe. The sediment sequence contains evidence of two periods characterized by downstream displacement of the estuarine/freshwater transitional boundary, dated to 3570–3400 cal. BP and 3300–3240 cal. BP. These are intercalated by one episode where marine influence shifted upstream. All sedimentation episodes developed under high terrestrial sediment delivery to this transitional region, leading to exceptionally high sedimentation rates, independently of the relative expression of terrestrial/marine influences in sediment facies. Our data show that these disturbances are mainly climate-driven and related to variations in rainfall and only secondarily with regional sea-level oscillations. From 3240 cal. BP onwards, an abrupt change in sediment facies is noted, in which the silting estuarine bottom reaches mean sea level and continued accreting until present under prevailing freshwater conditions, the tidal flat changing to an alluvial plain. The environmental modification is accompanied by a pronounced change in sedimentation rate that decreased by two orders of magnitude, reflecting the loss of accommodation space rather than the influence of climate or regional sea-level drivers.This work was developed in the scope of a BD grant funded by FCT (SFRH/BD/110270/2015) and under projects CoChange (HAR2014-51830-P) and COASTTRAN (HAR2011-29907-C03-00) financed by the Spanish Ministry of Science and Innovation and project Back to Sado (PTDC/HIS-ARQ/121592/2010) funded by FCT. The authors would also like to acknowledge the support of the Instituto Dom Luiz–IDL (UID/GEO/50019/2013)Portugal. Fundação para a Ciência e a Tecnologia; SFRH/BD/110270/2015Portugal. Fundação para a Ciência e a Tecnologia; PTDC/HIS-ARQ/121592/2010Portugal. Fundação para a Ciência e a Tecnologia; UID/GEO/50019/201

Controle de erosão hídrica para os solos de textura leve sob diferentes usos.

O Projeto IS_Agro foi proposto com um meio de avaliar a performance da agricultura brasileira a partir da estimativa e interpretação de diferentes indicadores agro-socioambientais que permitem a avaliação de políticas agrícolas na edificação de uma agricultura sustentável. O controle da erosão hídrica, é um desses indicadores

Levantamento de dados e mapeamento da erodibilidade do solo em escala nacional.

O objetivo do presente estudo foi determinar, em escala nacional, a erodibilidade do solo e elaborar um mapa do fator K para as principais classes de solo, considerando o primeiro nível

The role of climate, marine influence and sedimentation rates in late-Holocene estuarine evolution (SW Portugal)

Estuaries are sensitive to changes in global to regional sea level, to climate-driven variation in rainfall and to fluvial discharge. In this study, we use source and environmentally sensitive proxies together with radiocarbon dating to examine a 7-m-thick sedimentary record from the Sado estuary accumulated throughout the last 3.6 kyr. The lithofacies, geochemistry and diatom assemblages in the sediments accumulated between 3570 and 3240 cal. BP indicate a mixture between terrestrial and marine sources. The relative contribution of each source varied through time as sedimentation progressed in a low intertidal to high subtidal and low-energy accreting tidal flat. The sedimentation proceeded under a general pattern of drier and higher aridity conditions, punctuated by century-long changes of the rainfall regime that mirror an increase in storminess that affected SW Portugal and Europe. The sediment sequence contains evidence of two periods characterized by downstream displacement of the estuarine/freshwater transitional boundary, dated to 3570-3400 cal. BP and 3300-3240 cal. BP. These are intercalated by one episode where marine influence shifted upstream. All sedimentation episodes developed under high terrestrial sediment delivery to this transitional region, leading to exceptionally high sedimentation rates, independently of the relative expression of terrestrial/marine influences in sediment facies. Our data show that these disturbances are mainly climate-driven and related to variations in rainfall and only secondarily with regional sea-level oscillations. From 3240 cal. BP onwards, an abrupt change in sediment facies is noted, in which the silting estuarine bottom reaches mean sea level and continued accreting until present under prevailing freshwater conditions, the tidal flat changing to an alluvial plain. The environmental modification is accompanied by a pronounced change in sedimentation rate that decreased by two orders of magnitude, reflecting the loss of accommodation space rather than the influence of climate or regional sea-level drivers.FCT by Spanish Ministry of Science and Innovation [SFRH/BD/110270/2015, HAR2014-51830-P, HAR2011-29907-C03-00]FCTPortuguese Foundation for Science and Technology [PTDC/HISARQ/121592/2010]Instituto Dom Luiz-IDL [UID/GEO/50019/2013]info:eu-repo/semantics/publishedVersio

Limites e possibilidades da formação inicial para o desenvolvimento de práticas docentes autônomas

RESUMO Este artigo reúne bases empíricas de dois projetos de pesquisa mais amplos que focalizam as relações entre formação e profissão docente em cursos de Pedagogia e de Licenciatura em Ciências Biológicas. Objetivamos investigar os saberes mobilizados e⁄ou produzidos por professores especialistas e polivalentes para o desenvolvimento de práticas autônomas e sua relação com a formação inicial. Mobilizamos referenciais teóricos dos Saberes Docentes para a análise da autonomia docente sob um enfoque relacional em depoimentos de professores da educação básica. As informações sobre experiências formativas na graduação e na fase inicial da carreira do magistério foram adquiridas por meio de entrevistas e discussão coletiva e as análises buscaram problematizar relações entre formação, profissão e condicionantes que favorecem o desenvolvimento de práticas autônomas. Os resultados evidenciam fragilidades relativas à formação no que tange às práticas docentes, com destaque para: domínio de conteúdos e metodologias de ensino. Os depoimentos apontam, também, que determinados temas foram mal compreendidos ou despertaram pouco interesse na ocasião da graduação, mas passaram a fazer sentido ou a gerar inquietações após os embates com as exigências da escola. As interpretações dos depoimentos apontam para a necessidade de valorização da dimensão profissional da docência durante a formação inicial, como condição para o desenvolvimento de práticas autônomas