Gravity wave turbulence in a laboratory flume

We present an experimental study of the statistics of surface gravity wave turbulence in a flume of a horizontal size 12×6  m. For a wide range of amplitudes the wave energy spectrum was found to scale as Eω∼ω-ν in a frequency range of up to one decade. However, ν appears to be nonuniversal: it depends on the wave intensity and ranges from about 6 to 4. We discuss our results in the context of existing theories and argue that at low wave amplitudes the wave statistics is affected by the flume finite size, and at high amplitudes the wave breaking effect dominates

Formation-breakdown cycle of turbulent jets in a rotating fluid

Results of comprehensive particle image velocimetry measurements investigating the dynamics of turbulent jets in a rotating fluid are presented. It is observed that background system rotation induces a time-periodic formation–breakdown cycle of the jets. The flow dynamics associated with this process is studied in detail. It is found that the frequency of the cycle increases linearly with the background rotation rate. The data show that the onset of the breakdown phase and of the reformation phase of the cycle can be characterized in terms of a local Rossby number employing an internal velocity and a length scale of the jet. The critical values for this local Rossby number, for onset of breakdown and reformation, scale linearly with a global Rossby number based on the flow conditions at the source. The analysis of the experimental data suggests centrifugal instability as the potential origin of the formation–breakdown cycle

First hours of the GRB 030329 optical afterglow

We present the first results of the observations of the extremely bright optical afterglow of gamma-ray burst (GRB) 030329 with the 1.5m Russian-Turkish telescope RTT150 (TUBITAK National Observatory, Bakyrlytepe, Turkey). RTT150 was one of the first 1.5m-class telescopes pointed to the afterglow. Observations were started approximately 6 hours after the burst. During the first 5 hours of our observations the afterglow faded exactly as a power law with index -1.19+-0.01 in each of the BVRI Bessel filters. After that, in all BVRI filters simultaneously we observe a steepening of the power law light curve. The power law decay index smoothly approaches the value ~= -1.9, observed by other observatories later. This power law break occurs at t-t_0 =0.57 days and lasts for +-0.1 days. We observe no variability above the gradual fading with the upper limits 10--1% on time scales 0.1--1000s. Spectral flux distribution in four BVRI filters corresponds to the power law spectrum with spectral index \alpha=0.66+-0.01. The change of the power law decay index in the end of our observations can be interpreted as a signature of collimated ultrarelativistic jet. The afterglow flux distribution in radio, optical and x-rays is consistent with synchrotron spectrum. We continue our observations of this unique object with RTT150.Comment: Astronomy Letters, Vol. 29, No. 9, p. 573; 6 pages, 5 figures; pagination corrected; the original Russian version can be found at http://hea.iki.rssi.ru/~br/030329/pfh030329.pd

ENVIRONMENTALLY FRIENDLY METHOD OF GASEOUS FUEL COMBUSTION WITH THE USE OF QUASI-OPTICAL MICROWAVE

Subject of Research.The paper deals with the problem of developing low emission combustors operating on natural gas or LPG, to reduce emissions of nitrogen oxides NOx. The possibility of burning very lean fuel mixtures is studied. To initiate the ignition and combustion stabilization the discharge generated by the quasi-optical microwave is used. Main Results. Initiating ignition by streamer microwave discharge increases the rate of combustion and combustion efficiency about four times as compared with the conventional spark ignition. Streamer discharge ignition by very lean fuel-air mixture is demonstrated with the factor of oxiding agent excess greater than the limit of explosive range under normal conditions. According to indirect indicators, ignition by microwave discharge created by quasi-optical radiation is of non-thermal nature. Microwave discharge excites oxygen atoms, and intense ultra-violet radiation is generated as a result that causes formation of cold nonequilibrium plasma with avalanche growth of free electrons. Streamer discharge propagates at a speed of 5 km /s, so the initiation of the ignition occurs immediately throughout. The temperature of the fuel mixture at the point of ignition initiation does not exceed 400 К.There is no area with a temperature sufficient to initiate thermal Zeldovich mechanism of emission of nitrogen oxides. Combustion rate is high. As a result the Fenimore mechanism of "fast nitrogen oxides" has no chance to be progressing, and NOx emissions in appreciable quantities are excluded. Energy costs are comparable with spark ignition.Practical Relevance. The studied technology is designed for low emission internal combustion engines, power gas turbines, gas compressor units, fueled by natural gas

Inactivation of the transforming growth factor β type II receptor in human small cell lung cancer cell lines

Transforming growth factor β (TGF-β) exerts a growth inhibitory effect on many cell types through binding to two types of receptors, the type I and II receptors. Resistance to TGF-β due to lack of type II receptor (RII) has been described in some cancer types including small cell lung cancer (SCLC). The purpose of this study was to examine the cause of absent RII expression in SCLC cell lines. Northern blot analysis showed that RII RNA expression was very weak in 16 of 21 cell lines. To investigate if the absence of RII transcript was due to mutations, we screened the poly-A tract for mutations, but no mutations were detected. Additional screening for mutations of the RII gene revealed a GG to TT base substitution in one cell line, which did not express RII. This mutation generates a stop codon resulting in predicted synthesis of a truncated RII of 219 amino acids. The nature of the mutation, which has not previously been observed in RII, has been linked to exposure to benzo[a]-pyrene, a component of cigarette smoke. Since RII has been mapped to chromosome 3p22 and nearby loci are often hypermethylated in SCLC, it was examined whether the lack of RII expression was due to hypermethylation. Southern blot analysis of the RII promoter did not show altered methylation patterns. The restriction endonuclease pattern of the RII gene was altered in two SCLC cell lines when digested with Sma 1. However, treatment with 5-aza-2′-deoxycytidine did not induce expression of RII mRNA. Our results indicate that in SCLC lack of RII mRNA is not commonly due to mutations and inactivation of RII transcription was not due to hypermethylation of the RII promoter or gene. Thus, these data show that in most cases of the SCLC cell lines, the RII gene and promoter is intact in spite of absent RII expression. However, the nature of the mutation found could suggest that it was caused by cigarette smoking. © 1999 Cancer Research Campaig

Resveratrol inhibits benzo[a]pyrene–DNA adduct formation in human bronchial epithelial cells

Resveratrol ( trans-3,4’,5-trihydroxystilbene), a phytoalexin present in various plants and foods, has in several in vitro and in vivo studies demonstrated cancer chemopreventive and chemotherapeutic potential. We investigated the in vitro effect of resveratrol on benzo[ a] pyrene ( B[ a] P)-induced DNA adducts in human bronchial epithelial cells. This was compared to the effect of resveratrol on the expression of the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1 and the formation of B[ a] P metabolites. Exposure of BEAS-2B and BEP2D cells to B[ a] P and increasing concentrations of resveratrol resulted in a dose- and time-dependent inhibition of DNA adduct formation quantified by P-32-postlabelling. Supporting this result, resveratrol was shown to inhibit CYP1A1 and CYP1B1 gene expression, as measured by real-time reverse transcriptase - polymerase chain reaction. Also, a significant correlation was found between the number of DNA adducts and the mRNA levels of these genes. Using HPLC analysis, a concomitant decrease in the formation of B[ a]P-derived metabolic products was detected. In conclusion, these data lend support to a chemopreventive role of resveratrol in polycyclic aromatic hydrocarbon-induced carcinogenesis

Benzo[a]pyrene, Aflatoxine B1 and Acetaldehyde Mutational Patterns in TP53 Gene Using a Functional Assay: Relevance to Human Cancer Aetiology

Mutations in the TP53 gene are the most common alterations in human tumours. TP53 mutational patterns have sometimes been linked to carcinogen exposure. In hepatocellular carcinoma, a specific G>T transversion on codon 249 is classically described as a fingerprint of aflatoxin B1 exposure. Likewise G>T transversions in codons 157 and 158 have been related to tobacco exposure in human lung cancers. However, controversies remain about the interpretation of TP53 mutational pattern in tumours as the fingerprint of genotoxin exposure. By using a functional assay, the Functional Analysis of Separated Alleles in Yeast (FASAY), the present study depicts the mutational pattern of TP53 in normal human fibroblasts after in vitro exposure to well-known carcinogens: benzo[a]pyrene, aflatoxin B1 and acetaldehyde. These in vitro patterns of mutations were then compared to those found in human tumours by using the IARC database of TP53 mutations. The results show that the TP53 mutational patterns found in human tumours can be only partly ascribed to genotoxin exposure. A complex interplay between the functional impact of the mutations on p53 phenotype and the cancer natural history may affect these patterns. However, our results strongly support that genotoxins exposure plays a major role in the aetiology of the considered cancers

Somersault of Paramecium in extremely confined environments

We investigate various swimming modes of Paramecium in geometric confinements and a non-swimming self-bending behavior like a somersault, which is quite different from the previously reported behaviors. We observe that Paramecia execute directional sinusoidal trajectories in thick fluid films, whereas Paramecia meander around a localized region and execute frequent turns due to collisions with adjacent walls in thin fluid films. When Paramecia are further constrained in rectangular channels narrower than the length of the cell body, a fraction of meandering Paramecia buckle their body by pushing on the channel walls. The bucking (self-bending) of the cell body allows the Paramecium to reorient its anterior end and explore a completely new direction in extremely confined spaces. Using force deflection method, we quantify the Young’s modulus of the cell and estimate the swimming and bending powers exerted by Paramecium. The analysis shows that Paramecia can utilize a fraction of its swimming power to execute the self-bending maneuver within the confined channel and no extra power may be required for this new kind of self-bending behavior. This investigation sheds light on how micro-organisms can use the flexibility of the body to actively navigate within confined spaces