Endomembrane reorganization induced by heavy metals

Plant cells maintain plasmatic concentrations of essential heavy metal ions, such as iron, zinc, and copper, within the optimal functional range. To do so, several molecular mechanisms have to be committed to maintain concentrations of non-essential heavy metals and metalloids, such as cadmium, mercury and arsenic below their toxicity threshold levels. Compartmentalization is central to heavy metals homeostasis and secretory compartments, finely interconnected by traffic mechanisms, are determinant. Endomembrane reorganization can have unexpected effects on heavy metals tolerance altering in a complex way membrane permeability, storage, and detoxification ability beyond gene\u2019s expression regulation. The full understanding of endomembrane role is propaedeutic to the comprehension of translocation and hyper-accumulation mechanisms and their applicative employment. It is evident that further studies on dynamic localization of these and many more proteins may significantly contribute to the understanding of heavy metals tolerance mechanisms. The aim of this review is to provide an overview about the endomembrane alterations involved in heavy metals compartmentalization and tolerance in plants

Flexibility of Gender Stereotypes: Italian Study on Comparative Gender-consistent and Gender-inconsistent Information

The topic of this study is flexibility in gender stereotyping linked to attribution of toys, socio-cognitive traits, and occupations in 160 Italian children aged 6 to 12 years. We used the Gender Toys Choice, the Gender Traits Choice, and the Gender Jobs Choice, a selected set of colored cards containing masculine and feminine stimuli to assign to a male or female or both male and female silhouette (the flexible-choice technique). In order to verify the change of flexibility in gender stereotyping, we made use of four cartoon stories with male and female characters with typical or atypical traits and performing gender-consistent or gender-inconsistent activities. Results indicated that the exposure to cartoon stories with gender-inconsistent information rather than cartoon stories with gender-consistent information increased flexibility in gender stereotyping, showing age differences in favor of children aged 11-12. Implications in relation to the developmental-constructivist approach were noted

Slipping friction of an optically and magnetically manipulated microsphere rolling at a glass-water interface

The motion of submerged magnetic microspheres rolling at a glass-water interface has been studied using magnetic rotation and optical tweezers combined with bright-field microscopy particle tracking techniques. Individual microspheres of varying surface roughness were magnetically rotated both in and out of an optical trap to induce rolling, along either plain glass cover slides or glass cover slides functionalized with polyethylene glycol. It has been observed that the manipulated microspheres exhibited nonlinear dynamic rolling-while-slipping motion characterized by two motional regimes: At low rotational frequencies, the speed of microspheres free-rolling along the surface increased proportionately with magnetic rotation rate; however, a further increase in the rotation frequency beyond a certain threshold revealed a sharp transition to a motion in which the microspheres slipped with respect to the external magnetic field resulting in decreased rolling speeds. The effects of surface-microsphere interactions on the position of this threshold frequency are posed and investigated. Similar experiments with microspheres rolling while slipping in an optical trap showed congruent results.Comment: submitted to Journal of Applied Physics, 11 figure

Analysis of the Radish Seed Germination and Growth by Radiofrequency Stresses

In this work we report a study on the behavior of radish seed (Raphanus sativum L.) germination and growth under radiofrequency stresses. Groups of uniform seeds were irradiated at five duration time values of 60, 210, 375, 470 and 830 h at medium frequency of 1 Mhz, (MF/1), (MF/2), (MF/3), (MF/4) and (MF/5), respectively; at very high frequency of 100 Mhz, (VHF/1), (VHF/2) f(VHF/3), (VHF/4) and (VHF/5), respectively; at ultra high frequency of 900Mhz, (UHF/1), (UHF/2), (UHF/3), (UHF/4) and (UHF/5), respectively. The exhibited magnetic field for the three frequencies was of about 240 nT and the associate electric field inside the samples was less than 71 V/m, owing to the electric permittivity exposed sample. Another group of uniform radish seeds, irradiated by static magnetic (SM) field of 80 mT for the same time duration, was used as comparison, whereas untreated ones were used as control. The results showed that all physical stresses induced by magnetic fields did not have effect on seed germination as well as on cell elongation growth of the radish hypocotyls. On the contrary, a stimulating effect was observed on root growth

Effects of physical stresses on radish seed germination and growth

In this work we explore the consequences on variable magnetic fields applied to radish seeds (Raphanus sativum L.) on germination and seedling growth. Three, different alternate magnetic fields have been tested having a high intensity, 40 mT, at very low frequency, 0.2 Hz (B0.2), a low intensity, 577 µT, at low frequency, 370 kHz (B370), and a very low intensity, about 250 nT, at ultra high frequency, 900 MHz (RF900). These last generate electromagnetic waves which are nowadays very used for mobile communications. Groups of uniform radish seeds were exposed to alternate magnetic fields at three duration time values: i) 120 h (BO.2/1), 260 h (B0.2/2) and 360 h (B0.2/3) at high intensity and very low frequency, 0.2 Hz; ii) 120 h (B370/1), 260 h (B370/2), 360 h (B370/3) at low intensity and low frequency, 370 kHz; iii) 120 h (RF/1), 260 h (RF/2), 360 h (RF/3) at very low intensity and ultra high frequency, 900 MHz. Furthermore, another group of seeds was treated by laser beams of 248 nm wavelength whose magnetic field is coupled to an electric field. These two fields, by the Planck theory, origin the quantum energy, expresses by photons. Therefore, in the laser-matter interaction photons are absorbed dependent on laser shots. Again, we performed experiments at three duration time values that applied: 30 kshots (KrF/1), 80 kshots (KrF/2) and 135 kshots (KrF/3) at 40 mJcm2/ fluence. The magnetic field and electric field presented by the laser beam are approximatively 250 kVm/ and 850 µT, respectively. All treatments were performed at room temperature. Both untreated seeds and each group of seeds exposed to the physical stresses were transferred in Petri dishes and followed for their germination and seedling growth up to 96 h. The results showed that all physical stresses induced by alternate magnetic fields, radiofrequency and laser radiations did not have effect on seed germination as well as on cell elongation growth of the radish hypocotyls in comparison to control seeds. On the contrary, all physical stresses showed a significate stimulation on root growth

Actin and microtubules differently contribute to vacuolar targeting specificity during the export from the er

Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation. Considerable advances have been made in recent years in the comprehension of the relationship between the trans-Golgi network/early endosome (TGN/EE) and cytoskeletons, but studies have mainly focused on the transport to and from the plasma membrane. We address here the relationship of the cytoskeleton with different endoplasmic reticulum (ER) export mechanisms toward vacuoles. These emergent features of the plant endomembrane traffic are explored with an in vivo approach, providing clues on the traffic regulation at different levels beyond known proteins’ functions and interactions. We show how traffic of vacuolar markers, characterized by different vacuolar sorting determinants, diverges at the export from the ER, clearly involving different components of the cytoskeleton

Cardiometabolic Index (CMI) and Visceral Adiposity Index (VAI) Highlight a Higher Risk of Metabolic Syndrome in Women with Severe Obesity

Recent evidence shows that simple and inexpensive anthropometric measurements can be used to identify, at an early stage, women with obesity at increased risk of developing metabolic syndrome (MetS). Thus, the aim of this study was to compare the accuracy of five different indexes of adiposity and/or body composition in identifying MetS in a group of 876 women (mean age ± SD: 52.1 ± 13.8 years; body mass index (BMI): 43.6 ± 6.1 kg m−2). The following indexes were determined for each subject: waist-to-hip ratio (WHR), waist-to-height ratio (WtHR), body mass fat index (BMFI), visceral adiposity index (VAI), and cardiometabolic index (CMI). Overall, the presence of MetS was detected in 544 patients (62%). Pearson correlation coefficients were calculated to evaluate the relationships between body composition indexes and metabolic characteristics of the women. Receiver operating characteristic (ROC) analysis was used to determine the best predictor for each adiposity index among metabolic risk factors. The ROC analysis showed VAI (AUC = 0.84) and CMI (AUC = 0.86) showed the best performance in predicting MetS. Differences were found between the ROC area of CMI and VAI with all other indexes (p 0.92 for WHR, >0.76 for WtHR, >30.1 kg m−1 for BMFI, >1.94 for VAI, and >0.84 for CMI. In addition, VAI and CMI were the most sensitive and specific indexes compared with other indexes. In conclusion, VAI and CMI represent the most useful and reliable indexes to be used for detecting MetS in women suffering from obesity in clinical practice

Tomato oil encapsulation by α-, β-, and γ-Cyclodextrins: A comparative study on the formation of supramolecular structures, antioxidant activity, and carotenoid stability

Cyclodextrins (CDs) are oligosaccharides, comprising 6 (α), 7 (β), or 8 (γ) glucose residues, used to prepare oil-in-water emulsions and improve oil stability towards degradation. In this research, the aptitude of α-, β-, and γ-CDs to form complexes with a supercritical CO2 extracted lycopene-rich tomato oil (TO) was comparatively assessed. TO/CD emulsions and the resulting freeze-dried powders were characterized by microscopy, Fourier transform infrared-attenuated total reflection (FTIR-ATR), and differential scanning calorimetry (DSC), as well as for their antioxidant activity. Furthermore, carotenoid stability was monitored for 90 days at 25 and 4 ◦C. Confocal and SEM microscopy revealed morphological differences among samples. α- and β-CDs spontaneously associated into microcrystals assembling in thin spherical shells (cyclodextrinosomes, Ø ≈ 27 µm) at the oil/water interface. Much smaller (Ø ≈ 9 µm) aggregates were occasionally observed with γ-CDs, but most TO droplets appeared “naked”. FTIR and DSC spectra indicated that most CDs did not participate in TO complex formation, nevertheless structurally different interfacial complexes were formed. The trolox equivalent antioxidant capacity (TEAC) activity of emulsions and powders highlighted better performances of α- and β-CDs as hydrophobic antioxidants-dispersing agents across aqueous media. Regardless of CDs type, low temperature slowed down carotenoid degradation in all samples, except all-[E]-lycopene, which does not appear efficiently protected by any CD type in the long storage period

Bioactive composition and sensory evaluation of innovative spaghetti supplemented with free or α-cyclodextrin chlatrated pumpkin oil extracted by supercritical CO2

The feasibility of producing durum wheat pasta enriched with a lipophilic phytocomplex, extracted using supercritical carbon dioxide (SC-CO2), from ripe pumpkin, as free oil or as ready-to-mix oil/α-cyclodextrins (α-CDs)powder, was explored. Four types of pasta were prepared: (i)control spaghetti (S-CTRL); (ii)spaghetti supplemented with α-CDs (S-α-CD); (iii)spaghetti supplemented with pumpkin oil (S-Oil)and (iv)spaghetti supplemented with the pumpkin oil/α-CD powder (S-Oil/α-CD). The chemical, antioxidant, textural and sensory attributes of the different pasta were evaluated and compared. S-Oil and S-Oil/α-CD spaghetti were significantly enriched with phytosterols, squalene, carotenoids, tocochromanols and unsaturated fatty acids. Spaghetti containing α-CDs were slightly improved in terms of fiber content. Oil chlatration increased the stability of some bioactives during pasta production and ameliorated poor textural and sensory characteristics of the cooked spaghetti compared with S-Oil sample. S-Oil/α-CD spaghetti might be accepted by customers, if the potential health benefits were also explained