Characterization of Collagen from Different Discarded Fish Species of the West Coast of the Iberian Peninsula

13 páginas, 5 tablas, 3 figuras.-- This is an open Access article. Non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly attributed, cited, and is not altered, transformed, or built upon in any way, is permitted. The moral rights of the named author(s) have been asserted.Skin collagen of six discarded fish species was analyzed and compared. Acid soluble collagen (ASC) was extracted; a characteristic sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) profile for type I collagen was obtained, except for Chimaera mostrosa. Contents of collagen calculated from HPro (31.85% average) were higher than those determined from ASC extracts (17.75% average), with Galeus spp. being the species with the higher percentage. Amino acid analysis revealed the typical composition of collagen, with very few differences among species. Specific profiles were obtained after protease digestion. Denaturation temperature of ASC correlated well with imino and hydroxyproline contents. Results demonstrate the feasibility of using the obtained collagens in different industrial applicationsThis work was funded by European Union FEDER POCTEP Project 0330_IBEROMARE_1_P, European Union FEDER Atlantic Area Project MARMED-2011-1/164, Xunta de Galicia Project 10TAL011E, and a contract with the “Cooperativa de Armadores del Puerto de Vigo” and FROM (Ministery of Environment, Rural and Maritime Affairs, Spain)Peer reviewe

Valorization alternatives for a highly unused biomass (Small-spotted catshark discards and by-products) in the framework of LIFE iSEAS project

1 póster presentado en el Total Food Conference 2014 Science and technology for the economic and sustainable exploitation of agri-food chain wastes and co-products, Norwich, Uk, 11-13 November (2014)The authors thank the finnancial support received from the LIFE + Program of the European Union (LIFE Project‐ LIFE13 ENV/ES/000131N

Induction of Apoptosis and Cell Cycle Arrest in Human Colon Carcinoma Cells by Corema album Leaves

The leaves of Corema album (Ericaceae), an endemic shrub which grows in Atlantic coastal areas of the Iberian Peninsula, are rich in flavonoids and other secondary metabolites. Silica gel column chromatography of the ethyl acetate extract from dried leaves was performed and a flavonic active fraction was obtained. The cytotoxic activity of this fraction was assessed using the colon cancer cell lines HCT116 and HT29. After 48 hours of treatment, cell viability was determined with luminescence-based ATPLite assay, showing IC50 values of 7.2 ± 0.7 and 6.8 ± 1.2 µg/mL, respectively. The study by flow cytometry revealed that the cytotoxicity of this fraction was mediated, at least in part, by induction of apoptosis and G2/M cell cycle arrest. The active fraction was then subjected to Sephadex LH-20 chromatography and two flavonoids were separated and identified as the flavanone pinocembrin and 2’,4’-dihydroxychalcone after UV, MS and NMR analysis

Effect of soluble collagen hydrolysate from Prionace glauca skin in the expression of human fibroblast collagen

1 póster presentado al 5TH Trans-Atlantic Fisheries Technology Conference (45th) WEFTA MeetingSeafood discards and by-products represent a management and environmental problem for the fishery industry. In last European CFP (UE)1380/2013 regulation, stakeholders are encouraged to find alternative uses for these discards and subproducts different from direct human consumption. One potential for these materials is obtaining high value-added products such as proteins with technological properties (collagen and gelatins), peptides with functional properties (antimicrobial activities, antioxidant, antiproliferative and anti-hypertensive) or hemo-pigments (myoglobin).The authors are grateful to the “Programa Operativo FEDER, Cooperación Transfronteriza España-Portugal” for the financial support through the projet 0687_NOVOMAR_1P.Peer reviewe

Effects and Risk Assessment of the Polycyclic Musk Compounds Galaxolide (R) and Tonalide (R) on Marine Microalgae, Invertebrates, and Fish

The current research investigated the potential environmental risk of the polycyclic musk compounds, Galaxolide (R) (HHCB) and Tonalide (R) (AHTN), in the marine environments. These substances are lipophilic, bioaccumulated, and potentially biomagnified in aquatic organisms. To understand the toxicity of HHCB and AHTN, acute toxicity tests were performed by exposing marine microalgae (Phaeodactylum tricornutum, Tretraselmis chuii, and Isochrysis galbana), crustaceans (Artemia franciscana), echinoderms (Paracentrotus lividus), bivalves (Mytilus galloprovincialis), fish (Sparus aurata), and a candidate freshwater microalga (Raphidocelis subcapitata) to environmentally relevant concentrations (0.005-5 mu g/L) following standardized protocols (US EPA, Environment Canada and OECD). P. tricornutum and I. galbana were sensitive to both substances and for P. tricornutum exposed to HHCB and AHTN, the IC10 values (the inhibition concentration at which 10% microalgae growth inhibition was observed) were 0.127 and 0.002 mu g/L, respectively, while IC10 values calculated for I. galbana were 5.22 mu g/L (a little higher than the highest concentration) and 0.328 mu g/L, for HHCB and AHTN, respectively. Significant (p < 0.01) concentration dependent responses were measured in P. lividus and M. galloprovincialis larvae developments, as well as S. aurata mortality tested with HHCB. The effect of HHCB on P. lividus larvae development was the most sensitive endpoint recorded, producing an EC50 value (the effect concentration at which 50% effect was observed) of 4.063 mu g/L. Considering the risk quotients both substances seem to represent high environmental risk to P. tricornutum and M. galloprovincialis in marine environments

Asociación entre morfología del pie y rendimiento en gimnasia rítmica

[Abstract] The objectives of the study were to determine the influence of foot morphology on performance in Rhythmic Gymnastics and to analyze the influence of years of practice. The sample consisted of 48 gymnasts who had practiced federated gymnastics and competed during the last year. The results indicate that the gymnasts have predominantly a neutral foot and with a normal footprint, presenting enough asymmetry between feet, not significant, which may be a consequence of asymmetric work and should be corrected in training. Only the range of amplitude of the talocrural joint seems to be a characteristic of foot morphology that affects technical performance and seems more an innate characteristic. The practice of rhythmic gymnastics might not be as decisive a factor as could be supposed in the morphological modifications of the footprint.[Resumen] Los objetivos del estudio fueron determinar la influencia de la morfología del pie en el rendimiento en Gimnasia Rítmica y analizar la influencia de los años de práctica. La muestra estaba compuesta por 48 gimnastas que habían practicado gimnasia federada y competido durante el último año. Los resultados indican que las gimnastas tienen predominantemente un pie neutro y con huella normal, presentando bastante asimetría entre pies, no significativa, lo que puede ser consecuencia de un trabajo asimétrico y debería ser corregido en el entrenamiento. Sólo el rango de amplitud de la articulación talocrural parece ser una característica de la morfología del pie que incida sobre el rendimiento técnico y parece más una característica innata. La práctica de la Gimnasia Rítmica podría no ser un factor tan decisivo como podía suponerse en las modificaciones morfológicas de la huella plantar

Electrical characterization of single molecule and Langmuir-Blodgett monomolecular films of a pyridine-terminated oligo(phenylene-ethynylene) derivative

Monolayer Langmuir–Blodgett (LB) films of 1,4-bis(pyridin-4-ylethynyl)benzene (1) together with the “STM touch-to-contact” method have been used to study the nature of metal–monolayer–metal junctions in which the pyridyl group provides the contact at both molecule–surface interfaces. Surface pressure vs area per molecule isotherms and Brewster angle microscopy images indicate that 1 forms true monolayers at the air–water interface. LB films of 1 were fabricated by deposition of the Langmuir films onto solid supports resulting in monolayers with surface coverage of 0.98 × 10−9 mol·cm−2. The morphology of the LB films that incorporate compound 1 was studied using atomic force microscopy (AFM). AFM images indicate the formation of homogeneous, monomolecular films at a surface pressure of transference of 16 mN·m−1. The UV–vis spectra of the Langmuir and LB films reveal that 1 forms two dimensional J-aggregates. Scanning tunneling microscopy (STM), in particular the “STM touch-to-contact” method, was used to determine the electrical properties of LB films of 1. From these STM studies symmetrical I–V curves were obtained. A junction conductance of 5.17 × 10−5 G 0 results from the analysis of the pseudolinear (ohmic) region of the I–V curves. This value is higher than that of the conductance values of LB films of phenylene-ethynylene derivatives contacted by amines, thiols, carboxylate, trimethylsilylethynyl or acetylide groups. In addition, the single molecule I–V curve of 1 determined using the I(s) method is in good agreement with the I–V curve obtained for the LB film, and both curves fit well with the Simmons model. Together, these results not only indicate that the mechanism of transport through these metal–molecule–metal junctions is non-resonant tunneling, but that lateral interactions between molecules within the LB film do not strongly influence the molecule conductance. The results presented here complement earlier studies of single molecule conductance of 1 using STM-BJ methods, and support the growing evidence that the pyridyl group is an efficient and effective anchoring group in sandwiched metal–monolayer–metal junctions prepared under a number of different conditions.S. M., M. C. L, and P. C. are grateful for financial assistance from Ministerio de Economía y Competitividad from Spain in the framework of project CTQ2012-33198 and support from DGA and Fondos Feder for funding the Platon research group. S. M. also thanks his JIUZ-2013-CIE-05 grant. S. M and P. C. are grateful for the award of the CTQ2013-50187-EXP grant. P. J. L. thanks EPSRC for funding and also gratefully acknowledges support from the Australian Research Council (DP 140100855) and the award of the Future Fellowship (FT120100073). R. J. N. and S. J. H. thank EPSRC for funding (grant EP/H035184/1 and EP/K007785/1).Peer Reviewe

Overexpression of the ATPase Inhibitory Factor 1 Favors a Non-metastatic Phenotype in Breast Cancer

Partial suppression of mitochondrial oxidative phosphorylation and the concurrent activation of aerobic glycolysis is a hallmark of proliferating cancer cells. Overexpression of the ATPase inhibitory factor 1 (IF1), an in vivo inhibitor of the mitochondrial ATP synthase, is observed in most prevalent human carcinomas favoring metabolic rewiring to an enhanced glycolysis and cancer progression. Consistently, a high expression of IF1 in hepatocarcinomas and in carcinomas of the lung, bladder, and stomach and in gliomas is a biomarker of bad patient prognosis. In contrast to these findings, we have previously reported that a high expression level of IF1 in breast carcinomas is indicative of less chance to develop metastatic disease. This finding is especially relevant in the bad prognosis group of patients bearing triple-negative breast carcinomas. To investigate the molecular mechanisms that underlie the differential behavior of IF1 in breast cancer progression, we have developed the triple-negative BT549 breast cancer cell line that overexpresses IF1 stably. When compared to controls, IF1-cells partially shut down respiration and enhance aerobic glycolysis. Transcriptomic analysis suggested that migration and invasion were specifically inhibited in IF1-overexpressing breast cancer cells. Analysis of gene expression by qPCR and western blotting indicate that IF1 overexpression supports the maintenance of components of the extracellular matrix (ECM) and E-cadherin concurrently with the downregulation of components and signaling pathways involved in epithelial to mesenchymal transition. The overexpression of IF1 in breast cancer cells has no effect in the rates of cellular proliferation and in the cell death response to staurosporine and hydrogen peroxide. However, the overexpression of IF1 significantly diminishes the ability of the cells to grow in soft agar and to migrate and invade when compared to control cells. Overall, the results indicate that IF1 overexpression despite favoring a metabolic phenotype prone to cancer progression in the specific case of breast cancer cells also promotes the maintenance of the ECM impeding metastatic disease. These findings hence provide a mechanistic explanation to the better prognosis of breast cancer patients bearing tumors with high expression level of IF1.CN-T and IM-R were supported by pre-doctoral FPI-MEC and JAE-CSIC fellowships, respectively. This work was supported by grants from the Ministerio de Economía y Competitividad (SAF2013-41945-R; SAF2016-75916-R), Comunidad Madrid (S2011/BMD-2402), and Fundación Ramón Areces 2015, Spain.Peer reviewedPeer Reviewe

Structural control of the non-ionic surfactant alcohol ethoxylates (AEOs) on transport in natural soils

Surfactants, after use, enter the environment through diffuse and point sources such as irrigation with treated and non-treated waste water and urban and industrial wastewater discharges. For the group of non-ionic synthetic surfactant alcohol ethoxylates (AEOs), most of the available information is restricted to the levels and fate in aquatic systems, whereas current knowledge of their behavior in soils is very limited. Here we characterize the behavior of different homologs (C12-C18) and ethoxymers (E03, E06, and E08) of the AEOs through batch experiments and under unsaturated flow conditions during infiltration experiments. Experiments used two different agricultural soils from a region irrigated with reclaimed water (Guadalete River basin, SW Spain). In parallel, water flow and chemical transport were modelled using the HYDRUS-1D software package, calibrated using the infiltration experimental data. Estimates of water flow and reactive transport of all surfactants were in good agreement between infiltration experiments and simulations. The sorption process followed a Freundlich isotherm for most of the target compounds. A systematic comparison between sorption data obtained from batch and infiltration experiments revealed that the sorption coefficient (K-d) was generally lower in infiltration experiments, performed under environmental flow conditions, than in batch experiments in the absence of flow, whereas the exponent (beta) did not show significant differences. For the low clay and organic carbon content of the soils used, no clear dependence of K-d on them was observed. Our work thus highlights the need to use reactive transport parameterization inferred under realistic conditions to assess the risk associated with alcohol ethoxylates in subsurface environments. (C) 2020 The Authors. Published by Elsevier Ltd

Influencia de la formulación y de las variables de procesado sobre la estabilidad física de emulsiones de aceite esencial de tomillo

En la actualidad, los consumidores tienden a preferir productos que contengan compuestos naturales en lugar de aditivos sintéticos. Este hecho hace que en los últimos tiempos se haya realizado un gran esfuerzo en el estudio de formulaciones de emulsiones cuya fase orgánica está compuesta por aceites esenciales procedentes de plantas aromáticas. Estos aceites, como por ejemplo, el aceite esencial de tomillo (Thymus Vulgaris), constituido principalmente por timol, permiten diseñar productos más ecológicos y más seguros. En este estudio, se ha evaluado la estabilidad física de emulsiones concentradas de aceite de tomillo en función de la concentración de un tensioactivo de baja toxicidad y rápida biodegradación, Appyclean 6548. Dicho tensioactivo es no iónico del tipo alquil polipentósido, cuyo HLB está entre 9.0 y 9.5. En primer lugar, se obtuvo una emulsión primaria mediante el uso de un homogeneizador de alta velocidad rotor-stator, Silverson L5M. Posteriormente, se hizo pasar esta emulsión por un homogeneizador de alta presión por microcanales, Microfluidizer M110P. Finalmente, se estudió la estabilidad de las emulsiones resultantes mediante la combinación de técnicas como la difracción láser y la retrodispersión de luz en toda la longitud del recipiente que contenía la muestra.Nowadays, the consumers tend to prefer product containing natural components instead of synthetic additives. For this reason, recently the researchers have made a great effort to study formulation of emulsions whose organic phase containing essential oils from aromatic plants. These oils, such as, thyme essential oil (Thymus Vulgaris), consisting mainly of thymol, make possible to design more ecological and safer products. In this study, the physical stability of concentrated emulsions has been assessed as a function of a surfactant with low toxicity and rapid biodegradation, Appyclean 6548. This is a non-ionic surfactant, alkylpolypentoside, with HLB of 9.0-9.5. Firstly, a coarse emulsion was obtained by means of a rotor-stator homogenizer. Then, this emulsion was submitted at high pressure by a microchannel homogenizer. Finally, the stability of the resulting emulsions using multiple light scattering and laser diffraction.Plan Propio de la Universidad de Sevilla Proyecto: 2017/00000962Ministerio de Economía y Competitividad (España)/FEDER (UE) CTQ2015-70700-