Unsteady disturbances in a swept wing boundary layer due to plasma forcing

This work investigates the response of a transitional boundary layer to spanwise-invariant dielectric barrier discharge plasma actuator (PA) forcing on a 45 swept wing at a chord Reynolds number of 2:17 106. Two important parameters of the PA operation are scrutinized, namely, the forcing frequency and the streamwise location of forcing. An array of passive discrete roughness elements is installed near the leading edge to promote and condition a set of critical stationary crossflow (CF) instability modes. Numerical solutions of the boundary layer equations and linear stability theory are used in combination with the experimental pressure distribution to provide predictions of critical stationary and traveling CF instabilities. The laminar–turbulent transition front is visualized and quantified by means of infrared thermography. Measurements of velocity fields are performed using hotwire anemometry scans at specific chordwise locations. The results demonstrate the inherent introduction of unsteady velocity disturbances by the plasma forcing. It is shown that, depending on actuator frequency and location, these disturbances can evolve into typical CF instabilities. Positive traveling low-frequency type III modes are generally amplified by PA in all tested cases, while the occurrence of negative traveling high-frequency type I secondary modes is favored when PA is operating at high frequency and at relatively downstream locations, with respect to the leading edg

Assembly of the Auditory Circuitry by a Hox Genetic Network in the Mouse Brainstem

Rhombomeres (r) contribute to brainstem auditory nuclei during development. Hox genes are determinants of rhombomere-derived fate and neuronal connectivity. Little is known about the contribution of individual rhombomeres and their associated Hox codes to auditory sensorimotor circuitry. Here, we show that r4 contributes to functionally linked sensory and motor components, including the ventral nucleus of lateral lemniscus, posterior ventral cochlear nuclei (VCN), and motor olivocochlear neurons. Assembly of the r4-derived auditory components is involved in sound perception and depends on regulatory interactions between Hoxb1 and Hoxb2. Indeed, in Hoxb1 and Hoxb2 mutant mice the transmission of low-level auditory stimuli is lost, resulting in hearing impairments. On the other hand, Hoxa2 regulates the Rig1 axon guidance receptor and controls contralateral projections from the anterior VCN to the medial nucleus of the trapezoid body, a circuit involved in sound localization. Thus, individual rhombomeres and their associated Hox codes control the assembly of distinct functionally segregated sub-circuits in the developing auditory brainstem

Experimental base flow modification on a swept wing using plasma forcing

This work experimentally investigates plasma actuator (PA) forcing effects on the base flow and developing crossflow (CF) instabilities in a swept wing boundary layer. Spanwise-invariant plasma forcing near the leading edge is configured according to the base flow modification (BFM) strategy. A simplified predictive model is constructed by coupling an experimentally derived plasma body force and a linear stability theory and is used to infer the stability characteristics of the boundary layer subject to BFM. The base flow velocity is measured by stereo particle image velocimetry (PIV) at various PA operating conditions. Similarly, the developing CF instabilities, triggered through discrete roughness elements, are quantified by planar-PIV. The results demonstrate that a PA can reduce the boundary layer CF component, whereas the control authority shows a high dependence on the momentum coefficient. The dissimilar reduction between the streamline-aligned velocity and CF component leads to a local re-orientation of the base flow. Spanwise spectral analysis of the time-averaged flow indicates that stationary CF instabilities can be favorably manipulated whereas the BFM reduction effects depend on the corresponding initial amplitudes of stationary instabilities. An evident spanwise shift in the trajectory of stationary CF vortices is observed, which appears to result from the local alteration of the boundary layer stability due to the PA forcing. Despite the overall reduction in the amplitude of stationary CF instabilities, unsteady disturbances are found to be enhanced by the PA forcing. The current results shed light on the underlying principles of BFM-based PA operation in the context of laminar flow control

Acoustic wall treatments for wind tunnel aeroacoustic measurements

Sound absorbing porous materials are used to line a wind tunnel wall, in order to reduce reflections. However, the lining can have a detrimental effect on the acoustic measurements due to an increase in the noise radiated from the walls. In addition, the aerodynamic fidelity of the tunnel can be affected. In the present study, the influence of the porous materials on the boundary layer aerodynamic characteristics is assessed. The consequent aerodynamic noise scattering is also studied, and compared against the acoustic benefit from absorbing reflections in the test section. Geometric modelling is used to understand the influence of varying absorbing materials in reducing the acoustic interference caused by the reflections. The aerodynamic and acoustic results are related to the roughness, and to the viscous and inertial resistivities of the three porous materials studied. The material with highest roughness (polyester wool) is found to result in the strongest turbulent fluctuations in the boundary layer. However, it is the material with the thickest fibre diameter (PU foam), and consequent highest inertial resistivity, which generates the strongest surface noise scattering. Materials with high viscous resistivity, together with low inertial resistivity, are found to provide good sound absorbing capabilities. The results therefore indicate that the best choice of sound absorbing wall treatment for wind tunnel applications results from minimizing roughness and inertial resistivity, while maximizing viscous resistivity

Improvement of fatty acid profile and studio of rheological and technological characteristics in breads supplemented with flaxseed, soybean, and wheat bran flours

Functional breads constitute an interesting alternative as vehicle of new essential fatty acids sources. The aim of this study was to improve the fatty acids (FA) profile of bakery products, producing breads with low saturated fatty acid (SFA) content and with high polyunsaturated fatty acid (PUFA) content, through partial substitution of wheat flour by other ingredients (soy flour, flax flour, and wheat bran) and to analyze the effect of this change on the technological, rheological, and sensorial characteristics of breads. Flaxseed flour (FF), soybeans flour (SF), or wheat bran (WB) was used to replace 50, 100, and 150 g kg-1 of wheat flour (WF) in breads. FF or SF produced a decrease in monounsaturated and SFA and an increase of PUFA in these breads. Furthermore, breads replaced with FF presented considerable increase in the content of n3 FA, while, SF or WB contributed to rise of linoleic and oleic FA, respectively. The substitution percentage increase of FF, SF, or WB to formulation produced changes in the colour, rheological, textural, and technological characteristics of breads. This replacement resulted in improved lipid profile, being breads with 50 g kg-1 SF, the better acceptance, baking features, and enhanced fatty acid profile.Centro de Investigación y Desarrollo en Criotecnología de Alimento

Improvement of fatty acid profile and studio of rheological and technological characteristics in breads supplemented with flaxseed, soybean, and wheat bran flours

Functional breads constitute an interesting alternative as vehicle of new essential fatty acids sources. The aim of this study was to improve the fatty acids (FA) profile of bakery products, producing breads with low saturated fatty acid (SFA) content and with high polyunsaturated fatty acid (PUFA) content, through partial substitution of wheat flour by other ingredients (soy flour, flax flour, and wheat bran) and to analyze the effect of this change on the technological, rheological, and sensorial characteristics of breads. Flaxseed flour (FF), soybeans flour (SF), or wheat bran (WB) was used to replace 50, 100, and 150 g kg-1 of wheat flour (WF) in breads. FF or SF produced a decrease in monounsaturated and SFA and an increase of PUFA in these breads. Furthermore, breads replaced with FF presented considerable increase in the content of n3 FA, while, SF or WB contributed to rise of linoleic and oleic FA, respectively. The substitution percentage increase of FF, SF, or WB to formulation produced changes in the colour, rheological, textural, and technological characteristics of breads. This replacement resulted in improved lipid profile, being breads with 50 g kg-1 SF, the better acceptance, baking features, and enhanced fatty acid profile.Centro de Investigación y Desarrollo en Criotecnología de Alimento

Effect of Sugars on Gelation Kinetics of Gelatin Gels

We investigate the rheological behavior of aqueous solutions containing animal gelatin, sugars and polyols. The aim is to study how the gelation kinetics, transition temperatures and gel strengths of an aqueous gelatin solution can be affected by the progressive addition of co-solutes. Aqueous solutions with a fixed mass percentage of gelatin of 6.8 wt% were prepared at various concentrations of sugars and polyols. Through Dynamic Temperature Ramp tests, performed at various ramp rates, and Dynamic Time Sweep and Dynamic Frequency Sweep tests, carried out at different temperatures, it was possible both to evaluate the transition temperatures and to monitor the gelation kinetics of the samples. It was found that the contribution of co-solutes positively affects both the gelation process and the thermal stability of the aqueous gelatin solution by reducing the gelation time and improving the mechanical properties of the gel in terms of network elasticity

Involvement of the protein ras homolog enriched in the striatum, rhes, in dopaminergic neurons’ degeneration: Link to parkinson’s disease

Rhes is one of the most interesting genes regulated by thyroid hormones that, through the inhibition of the striatal cAMP/PKA pathway, acts as a modulator of dopamine neurotransmission. Rhes mRNA is expressed at high levels in the dorsal striatum, with a medial‐to‐lateral expression gradient reflecting that of both dopamine D2 and adenosine A2A receptors. Rhes transcript is also present in the hippocampus, cerebral cortex, olfactory tubercle and bulb, substantia nigra pars compacta (SNc) and ventral tegmental area of the rodent brain. In line with Rhes‐dependent regulation of dopaminergic transmission, data showed that lack of Rhes enhanced cocaine‐ and am-phetamine‐induced motor stimulation in mice. Previous studies showed that pharmacological de-pletion of dopamine significantly reduces Rhes mRNA levels in rodents, non‐human primates and Parkinson’s disease (PD) patients, suggesting a link between dopaminergic innervation and physiological Rhes mRNA expression. Rhes protein binds to and activates striatal mTORC1, and modulates L‐DOPA‐induced dyskinesia in PD rodent models. Finally, Rhes is involved in the survival of mouse midbrain dopaminergic neurons of SNc, thus pointing towards a Rhes‐dependent modulation of autophagy and mitophagy processes, and encouraging further investigations about mechanisms underlying dysfunctions of the nigrostriatal system

Redescription of Cardiosporidium cionae (Van Gaver and Stephan, 1907) (Apicomplexa: Piroplasmida), a plasmodial parassite of ascidian haemocytes

Cardiosporidium cionae (Apicomplexa), from the ascidian Ciona intestinalis L., is redescribed with novel ultrastructural, phylogenetic and prevalence data. Ultrastructural analysis of specimens of C. intestinalis collected from the Gulf of Naples showed sporonts and plasmodia of C. cionae within the host pericardial body. Several merogonic stages and free merozoites were found in the pericardial body, together with sexual stages. All stages showed typical apicomplexan cell organelles, i.e. apicoplasts, rhoptries and subpellicular microtubules. Merogonic stages of C. cionae were also produced inside haemocytes. A fragment of the rSSU gene of C. cionae was amplified by PCR using DNA extracted from the pericardial bodies. The amplified product showed closest affinity with other apicomplexan representatives and a 66 bp unique insertion, specific for C. cionae, at position 1644. Neighbour-joining phylogenetic analysis placed C. cionae in a clade with other piroplasm genera, including Cytauxzoon, Babesia and Theileria spp. The parasite was found in different populations of C. intestinalis with highest prevalence in October–November. Ultrastructural and DNA data showed that the organism, described in 1907 from the same host but not illustrated in detail, is a member of a novel marine apicomplexan radiation of tunicate parasites

Lattice Boltzmann very large eddy simulations of a turbulent flow over covered and uncovered cavities

Microphone measurements in a closed test section wind tunnel are affected by turbulent boundary layer (TBL) pressure fluctuations. These fluctuations are mitigated by placing the microphones at the bottom of cavities, usually covered with a thin, acoustically transparent material. Prior experiments showed that the cavity geometry affects the propagation of TBL pressure fluctuations toward the bottom. However, the relationship between the cavity geometry and the flowfield within the cavity is not well understood. Therefore, a very large-eddy simulation was performed using the lattice Boltzmann method. A cylindrical, a countersunk and a conical cavity are simulated with and without a fine wire-cloth cover, which is modeled as a porous medium governed by Darcy’s law. Adding a countersink to an uncovered cylindrical cavity is found to mitigate the transport of turbulent structures across the bottom by shifting the recirculation pattern away from the cavity bottom. Covering the cavities nearly eliminates this source of hydrodynamic pressure fluctuations. The eddies within the boundary layer, which convect over the cover, generate a primarily acoustic pressure field inside the cavities and thus suggesting that the pressure fluctuations within covered cavities can be modeled acoustically. As the cavity diameter increases compared to the eddies’ integral length scale, the amount of energy in the cut-off modes increases with respect to the cut-on modes. Since cut-off modes decay as they propagate into the cavity, more attenuation is seen. The results are in agreement with experimental evidenc