EFFECT OF NATURAL ANTIOXIDANT OF CAKE QUALITY DURING STORAGE

In this study, both the methanol extraction of sage leaves and clove buds were added as natural antioxidant at concentraction 100, 200 and 300 ppm from butter weight using in cake production. The cake produced was storage at room tempera-ture for 8 weeks. Butter stability was measured by Rancimat apparatus. Also, perox-ide value, acid value and thiobarbituric acid value were measured. Sensory evalua-tion was measured directly after baking (before storage) and at the end of storage pe-riod. Rancimat results showed that increasing natural antioxidant concentration led to longer butter stability and retarded rancidity than control. At the same time sage leaves extraction was much effectiveness for period long butter stability compared to clove buds extraction. Also the results revealed that the more concentration of natural antioxidants increased the peroxide value, acid value and thiobarbituric aicd value decreased compared to control. Sensory evaluation in zero time indicated im-provement in taste, flavor, texture and tenderness while color values were decreased with increasing sage and clove extraction compared to control. After 8 weeks of storage sensory evaluation scores were generally decreased

Food Technology Research Institute, Agricultural Research Center, Giza, Egypt

Day by day increase the importance of using the natural food additives. Hence in this study, the phenolic extracted from basil leaves, spearmint leaves and fennel seeds, as natural antioxidants, were added at concentrations 200, 400 and 600 ppm from fat weight using in biscuit production. Biscuit was stored at room temperature for 8 months. Induction period was measured by Rancimat apparatus. Total phenolic and phenolic acids were determined. Peroxide value, acid value, thiobarbituric acid and refractive index were measured during storage. Antimicrobial activity of phenolic extraction were examined after 8 months. Sensory evaluation was measured directly after baking. The results showed that total phenolic content in basil leaves, spearmint leaves and fen-nel seeds were 3.97, 1.91 and 1.53 mg/g as caffeic acid equivalents respectively. Five phenolic acids were found in both basil leaves and spearmint leaves, while four phenolic acids were found in fennel seeds as determined by using HPLC. Rancimat results showed that induction period for phenolic herbs could be ranked as follow: basil leaves > spearmint leaves > fennel seeds. The re-sults elucidated that the best concentration from natural additives as antioxidants activity were 400 and 600 ppm compared with BHT. The results revealed that with increasing the concentration of natural antioxidants increased, the more peroxide value, acid value, thiobarbituric acid and refractive index were decreased. Total bacterial count and (yeast & mold) count were decreased with increas-ing the concentration phenolic additives. Sensory evaluation indicated that addition of different anti-oxidants showed no significant differences be-tween control and biscuit sample

Preparation and Biological Evaluation of 99mtc-Sarafloxacin and 99mtc- Danofloxacin Complexes as a Model for Infection Imaging

Infection and inflammation remain a major cause of mortality and morbidity globally. This promotes research into better and more accurate diagnostic and therapeutic methods. This investigation focused on the labeling of sarafloxacin and danofloxacin for infection imaging. The radiolabeled antibiotic 99mTc-sarafloxacin and 99mTc-danofloxacin were assessed as an infection imaging agent in a mouse model. 99mTc-sarafloxacin and 99mTc-danofloxacin were obtained at pH 11 with a radiochemical yield of 96, 90%, respectively by adding 99mTc to 1 mg sarafloxacin or danofloxacin in the presence of 50 μg SnCl2.2H2O. Biodistribution studies in mice were carried out in experimentally induced infection in the left thigh using Staphylococcus aureus. Both thighs of the mice were dissected and counted, and the ratio of bacterial infected thigh/contralateral thigh was then evaluated. 99mTc-sarafloxacin and 99mTc-danofloxacin showed high uptake (T/NT=3.8±0.1 and 4.9±0.1, respectively) in the infectious lesion and abscess to normal muscle ratio indicating that 99mTcsarafloxacin and 99mTc-danofloxacin could be used for infection imaging

Preparation and Biological Evaluation of 99mtc-Sarafloxacin and 99mtc- Danofloxacin Complexes as a Model for Infection Imaging

Infection and inflammation remain a major cause of mortality and morbidity globally. This promotes research into better and more accurate diagnostic and therapeutic methods. This investigation focused on the labeling of sarafloxacin and danofloxacin for infection imaging. The radiolabeled antibiotic 99mTc-sarafloxacin and 99mTc-danofloxacinwere assessed as an infection imaging agent in a mouse model. 99mTc-sarafloxacin and 99mTc-danofloxacin were obtained at pH 11 with a radiochemical yield of 96, 90%, respectively by adding 99mTc to 1 mg sarafloxacin or danofloxacin in the presence of 50 μg SnCl2.2H2O. Biodistribution studies in mice were carried out in experimentally induced infection in the left thigh using Staphylococcus aureus. Both thighs of the mice were dissected and counted and the ratio of bacterial infected thigh/contralateral thigh was then evaluated. 99mTc-sarafloxacin and 99mTc-danofloxacin showed high uptake (T/NT=3.8±0.1 and 4.9±0.1, respectively) in the infectious lesion and abscess to normal muscle ratio indicating that 99mTcsarafloxacin and 99mTc-danofloxacin could be used for infection imaging.Key words: sarafloxacin/ danofloxacin/ Technetium-99m/ Infection/ inflammation/ Diagnosi

Spectroscopic, thermodynamic, kinetic studies and oxidase/antioxidant biomimetic catalytic activities of tris(3,5-dimethylpyrazolyl)borate Cu(II) complexes

A series of copper(II) complexes, viz. [TpMeMeCu(Cl)(H2O)] (1), [TpMeMeCu(OAc)(H2O)] (2), [TpMeMeCu(NO3)] (3) and [TpMeMeCu(ClO4)] (4) containing tris(3,5-dimethylpyrazolyl)borate (KTpMeMe), have been synthesized and fully characterized. The substitution reaction of 1 with thiourea was studied under pseudo-first-order conditions as a function of concentration, temperature and pressure in methanol and acetonitrile as solvents. Two reaction steps that both depended on the nucleophile concentration were observed for both solvents. Substitution of coordinated methanol is about 40 times faster than the substitution of chloride. In acetonitrile, the rate constant for the displacement of coordinated acetonitrile was more than 20 times faster than the substitution of chloride. The reported activation parameters indicate that both reaction steps follow a dissociative mechanism in both solvents. On going from methanol to acetonitrile, the rate constant for the displacement of the solvent becomes more than 200 times faster due to the more labile acetonitrile, but the substitution mechanism remained to have a dissociative character. The antioxidant activities of 1–4 were evaluated for superoxide dismutase (SOD), glutathione-s-transferase (GST0 and glutathione reduced (GSH-Rd) activity. 1 and 2 were found to show (p < 0.05) the highest antioxidant activity in comparison to 3 and 4, which can be ascribed to the geometric configuration as well as the nature of the co-ligand. 1 showed catechol oxidase activity with turnover numbers of 20 min−1 and a coordination affinity for 3,5-DTBC of K1, = 31 mM−1. K1 is rather large and seems to be typical for faster biomimetic models, and also for the enzyme itself (25 mM−1). The reaction rate depended linearly on the complex concentration, indicating a first-order dependence on the catalyst concentration

Tissue p53-induced glycolysis and apoptosis regulator (TIGAR) is associated with oxidative stress in benign and malignant colorectal lesions

Background: Colorectal cancer (CRC) is the fourth leading cause of cancer-mortality worldwide. Tissue p53-induced glycolysis and apoptosis regulator gene (TIGAR) has an important role in cellular glycolysis and acts as an oncogene.Objectives: We aimed to investigate the diagnostic utility of TIGAR in both CRC and benign bowel deceases.Methods: One-hundred-eighty tissue samples were recruited and classified into 3 groups: group (1) 60 CRC samples from the tumor mass of colorectal cancer patients, group (2), 60 non-neoplastic colorectal tissue samples and group (3), 60 benign bowel lesions samples (ulcerative-colitis, Chron’s disease, adenoma, and familial adenomatous polyposis). The expressions of tissue mRNA and protein levels of TIGAR were determined. Levels of malondialdehyde and reduced glutathione were also measured.Results: Our results showed upregulated expressions of TIGAR gene and protein levels in CRC tissues and benign colonic lesions compared to non-tumor tissues (p &lt; 0.0001). Their levels were higher in inflammatory bowel diseases compared to non-inflammatory benign lesions. There were significant relations among TIGAR expression, protein levels, TNM staging, and the presence of metastasis (p&lt;0.0001). ROC curve analysis showed that TIGAR mRNA expression and its protein can discriminate between CRC and benign lesions and between benign bowel disease and controls.Conclusions: To the best of our knowledge this is the first study to assess the level of TIGAR in different benign bowel diseases. TIGAR might be involved in the pathogenesis of benign and malignant bowel diseases and could be a potential biomarker for diagnosis

BSA Interaction, Molecular Docking, and Antibacterial Activity of Zinc(II) Complexes Containing the Sterically Demanding Biomimetic N3S2 Ligand: The Effect of Structure Flexibility

Two zinc(II) complexes, DBZ and DBZH4, that have (ZnN3S2) cores and differ in the bridging mode of the ligating backbone, effectively bind to BSA. The binding affinity varies as DBZ > DBZH4 and depends on the ligand structure. At low concentrations, both complexes exhibit dynamic quenching, whereas at higher concentrations they exhibit mixed (static and dynamic) quenching. The energy transfer mechanism from the BSA singlet excited state to DBZ and DBZH4, is highly likely according to steady-state fluorescence and time-correlated singlet photon counting. Molecular docking was used to support the mode of interaction of the complexes with BSA and showed that DBZ had more energy for binding. Furthermore, antibacterial testing revealed that both complexes were active but to a lesser extent than chloramphenicol. In comparison to DBZH4, DBZ has higher antibacterial activity, which is consistent with the binding constants, molecular docking, and particle size of adducts. These findings may have an impact on biomedicine

Callus induction and regeneration of Stevia rebaudiana Bertoni

Abstract: Stevia rebaudiana Bertoni is an important non-caloric sweetening herb.It has some kind of diterpenoidsteviol glycosides that had no negative effect on blood sugar level.In the present study, efficient plant regeneration via callus was established. Explants were cultured on MS supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) alone and the combination with 1.0 mg/L BA were used to initiate callus. Maximum frequency of callus induction (89%)were observed on MS supplemented with 2.0 mg/L2,4-D.Multiple shoots were obtained on MS medium containing 0.5 mg/L BA and 1.0 mg/LNAA from friable, granule and healthy calli which obtained after 45 days.Regeneration percentage, number of shootlets/ callus part, length of shootlets, number of nodes and number ofleaveswere 86 %, 13.2, 4.36 cm, 4.0 and 8.0, respectively