Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31

A purification procedure for a new kind of extradiol dioxygenase, termed chlorocatechol 2,3-dioxygenase, that converts 3-chlorocatechol productively was developed. Structural and kinetic properties of the enzyme, which is part of the degradative pathway used for growth of Pseudomonas putida GJ31 with chlorobenzene, were investigated. The enzyme has a subunit molecular mass of 33.4 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Estimation of the native Mr value under nondenaturating conditions by gel filtration gave a molecular mass of 135 ± 10 kDa, indicating a homotetrameric enzyme structure (4 × 33.4 kDa). The pI of the enzyme was estimated to be 7.1 ± 0.1. The N-terminal amino acid sequence (43 residues) of the enzyme was determined and exhibits 70 to 42% identity with other extradiol dioxygenases. Fe(II) seems to be a cofactor of the enzyme, as it is for other catechol 2,3-dioxygenases. In contrast to other extradiol dioxygenases, the enzyme exhibited great sensitivity to temperatures above 40°C. The reactivity of this enzyme toward various substituted catechols, especially 3-chlorocatechol, was different from that observed for other catechol 2,3-dioxygenases. Stoichiometric displacement of chloride occurred from 3-chlorocatechol, leading to the production of 2-hydroxymuconate.

Uniqueness properties of the Kerr metric

We obtain a geometrical condition on vacuum, stationary, asymptotically flat spacetimes which is necessary and sufficient for the spacetime to be locally isometric to Kerr. Namely, we prove a theorem stating that an asymptotically flat, stationary, vacuum spacetime such that the so-called Killing form is an eigenvector of the self-dual Weyl tensor must be locally isometric to Kerr. Asymptotic flatness is a fundamental hypothesis of the theorem, as we demonstrate by writing down the family of metrics obtained when this requirement is dropped. This result indicates why the Kerr metric plays such an important role in general relativity. It may also be of interest in order to extend the uniqueness theorems of black holes to the non-connected and to the non-analytic case.Comment: 30 pages, LaTeX, submitted to Classical and Quantum Gravit

A Proof of Uniqueness of the Taub-bolt Instanton

We show that the Riemannian Schwarzschild and the ``Taub-bolt'' instanton solutions are the only spaces (M,g) such that 1) M is a 4-dimensional, simply connected manifold with a Riemannian, Ricci-flat C^2-metric g which admits (at least) a 1-parameter group of isometries H without isolated fixed points on M. 2) The quotient (M L)/H (where L is the set of fixed points of H) is an asymptotically flat manifold, and the length of the Killing field corresponding to H tends to a constant at infinity.Comment: 20 pages. LaTeX. Substantially extended and correcte

A spacetime characterization of the Kerr metric

We obtain a characterization of the Kerr metric among stationary, asymptotically flat, vacuum spacetimes, which extends the characterization in terms of the Simon tensor (defined only in the manifold of trajectories) to the whole spacetime. More precisely, we define a three index tensor on any spacetime with a Killing field, which vanishes identically for Kerr and which coincides in the strictly stationary region with the Simon tensor when projected down into the manifold of trajectories. We prove that a stationary asymptotically flat vacuum spacetime with vanishing spacetime Simon tensor is locally isometric to Kerr. A geometrical interpretation of this characterization in terms of the Weyl tensor is also given. Namely, a stationary, asymptotically flat vacuum spacetime such that each principal null direction of the Killing form is a repeated principal null direction of the Weyl tensor is locally isometric to Kerr.Comment: 23 pages, No figures, LaTeX, to appear in Classical and Quantum Gravit

Uniqueness Theorem for Generalized Maxwell Electric and Magnetic Black Holes in Higher Dimensions

Based on the conformal energy theorem we prove the uniqueness theorem for static higher dimensional electrically and magnetically charged black holes being the solution of Einstein (n-2)-gauge forms equations of motion. Black hole spacetime contains an asymptotically flat spacelike hypersurface with compact interior and non-degenerate components of the event horizon.Comment: 7 pages, RevTex, to be published in Phys.Rev.D1

Uniqueness Theorem for Static Black Hole Solutions of sigma-models in Higher Dimensions

We prove the uniqueness theorem for self-gravitating non-linear sigma-models in higher dimensional spacetime. Applying the positive mass theorem we show that Schwarzschild-Tagherlini spacetime is the only maximally extended, static asymptotically flat solution with non-rotating regular event horizon with a constant mapping.Comment: 5 peges, Revtex, to be published in Class.Quantum Gra

Activity in human reward-sensitive brain areas is strongly context dependent.

Contains fulltext : 55984.pdf (Publisher’s version ) (Closed access)Functional neuroimaging research in humans has identified a number of brain areas that are activated by the delivery of primary and secondary reinforcers. The present study investigated how activity in these reward-sensitive regions is modulated by the context in which rewards and punishments are experienced. Fourteen healthy volunteers were scanned during the performance of a simple monetary gambling task that involved a "win" condition (in which the possible outcomes were a large monetary gain, a small gain, or no gain of money) and a "lose" condition (in which the possible outcomes were a large monetary loss, a small loss, or no loss of money). We observed reward-sensitive activity in a number of brain areas previously implicated in reward processing, including the striatum, prefrontal cortex, posterior cingulate, and inferior parietal lobule. Critically, activity in these reward-sensitive areas was highly sensitive to the range of possible outcomes from which an outcome was selected. In particular, these regions were activated to a comparable degree by the best outcomes in each condition-a large gain in the win condition and no loss of money in the lose condition-despite the large difference in the objective value of these outcomes. In addition, some reward-sensitive brain areas showed a binary instead of graded sensitivity to the magnitude of the outcomes from each distribution. These results provide important evidence regarding the way in which the brain scales the motivational value of events by the context in which these events occur

Stationary Black Holes: Uniqueness and Beyond

The spectrum of known black-hole solutions to the stationary Einstein equations has been steadily increasing, sometimes in unexpected ways. In particular, it has turned out that not all black-hole-equilibrium configurations are characterized by their mass, angular momentum and global charges. Moreover, the high degree of symmetry displayed by vacuum and electro-vacuum black-hole spacetimes ceases to exist in self-gravitating non-linear field theories. This text aims to review some developments in the subject and to discuss them in light of the uniqueness theorem for the Einstein-Maxwell system.Comment: Major update of the original version by Markus Heusler from 1998. Piotr T. Chru\'sciel and Jo\~ao Lopes Costa succeeded to this review's authorship. Significantly restructured and updated all sections; changes are too numerous to be usefully described here. The number of references increased from 186 to 32