Effect of sumac extract on serum oxidative status, RANKL/OPG system and alveolar bone loss in experimental periodontitis in rats

Objectives Sumac (Rhus coriaria L.) is widely used spice which has several properties such as antioxidant, anti-inflammatory and antimicrobial. The purpose of this animal study was to evaluate the effects of sumac extract on levels of receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG) expression, serum oxidative status, and alveolar bone loss in experimental periodontitis. Material and Methods Twenty-four Wistar rats were separated into three groups: non-ligated (NL, n=8), ligature only (LO, n=8), and ligature and treated with sumac extract (S, n=8) (20 mg/kg per day for 11 days). A 4/0 silk suture was placed around the mandibular right first molars subgingivally; after 11 days, the rats were sacrificed, and alveolar bone loss was histometrically measured. The detection of RANKL and OPG were immunohistochemically performed. Levels of serum total antioxidant status (TAS)/total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed. Results Alveolar bone loss was significantly greater in the LO group compared to the S and NL groups (

The influence of ?-Tocopherol on alveolar bone loss in diabetic rats with experimental periodontitis

Amaç: Birçok deneysel ve klinik çalışmada diyabetin periodonsiyum üzerinde yaptığı değişiklikler değerlendirilirken bunun yanında periodontitisin diyabet durumuna etkiside araştırılmıştır. Bu araştırmanın amacı, deneysel periodontitis ve streptozotosin (STZ) ile diyabet oluşturulan/oluşturulmayan sıçanlarda ?-tokoferol uygulamasının alveoler kemik kaybı üzerindeki etkilerini araştırmaktır. Gereç ve Yöntemler: Çalışmaya kırk adet erkek Sprague Dawley sıçana dahil edildi ve anestezi uygulandıktan sonra mandibular sağ birinci molar dişlere subgingival olarak 3/0 ipek sütur bağlandı. Bu hayvanlar, sağlıklı grup (Grup I) ve tek seferde 50 mg/kg STZ enjekte edilen diyabetik grup (Grup II) olmak üzere iki gruba ayrıldı. Bu gruplarda serum fizyolojik (Grup IA , Grup IIA) ve ?-tokoferol (40mg/kg/gün) enjekte edilen alt gruplara (Grup IB, Grup IIB) ayrıldı. Ligatür bağlandıktan 3 hafta sonra sıçanlar kurban edildi ve dokular histometrik ölçümler için nötral tamponlanmış %10luk formaldehit solüsyonunda sabitlendi. Alveoler kemik kaybı (AKK), görüntü analiz programı kullanılarak analiz edildi. İstatistiksel kıyaslamalar için tek-yönlü varyans analizi, kullanıldı p 0.05 anlamlılık düzeyi kabul edildi. Bulgular: Histometrik incelemeden sonra veriler değerlendirildi ve istatistiksel olarak gruplar arasında AKK düzeyleri farklı bulunmadı (p0.05). Sonuçlar: Verilerin değerlendirilmesi sonucunda ?-tokoferol uygulamasının deneysel periodontitisli STZ ile diyabet oluşturulmuş/oluşturulmamış sıçanlarda AKK üzerinde bir etki oluşturmadığı gösterildi.Objectives: A variety of clinical and experimental studies investigated the influence of diabetes on the periodontium, as well as the impact of periodontitis on diabetes status. The aim of this study was to investigate the effects of &amp;#945;-tocopherol on alveolar bone loss in rats with experimental periodontitis with/without streptozotocin (STZ)-induced diabetes. Materials and Methods: Forty male Sprague Dawley rats were included in the study and after anesthesia 3/0 silk sutures were placed at the subgingival level of the mandibular right first molars. These animals were divided into two groups: Healty group (Group I) and diabetic group by single injection of 50 mg/kg STZ (Group II). These groups divided into two subgroups; twenty rats were saline group (Group IA, Group IIA) and other twenty rats (Group IB, Group IIB) were &amp;#945;-tocopherol (40mg/kg/day) group. After 3 weeks, all rats were sacrified and the tissues were fixed using neutral buffered 10% formaldehyde solution for histometrical investigation. Alveolar bone loss (ABL), were analyzed by using image analysis program. Statistical comparisons were performed by using one-way analysis of variance with significance set at p>0.05. Results: Data were evaluated after the histometrical examination and there were no statistically significant differences in ABL between the study groups (p<0.05). Conclusions: Analysis of these data demonstrated that &amp;#945;-tocopherol treatment was not affected the ABL of rats with experimental periodontitis with/without STZ-induced diabetes

CLINICAL ANALYSIS OF DESQUAMATIVE GINGIVITIS RELATED ORAL MUCOCUTANEOUS DISEASES

Objectives: Several mucocutaneous diseases are responsible for desquamative gingivitis (DG) and diagnostic delays are common in these diseases due to non-specific clinical oral presentations. The present study aimed to analyze the clinical features of DG-related mucocutaneous diseases with oral manifestations and compare the findings with previous reports. Materials and Methods: Thirty newly diagnosed patients with oral mucocutaneous disease in the Dermatology and Venereology Department of Akdeniz University Medical School, between January 2018 and January 2020, and referred to the Periodontology Department for periodontal treatment were evaluated retrospectively. Patients demographic data (age and gender), medical comorbidities, primary lesion site (oral or skin), and clinical features of gingival and oral lesions (location and symptoms) were examined. Results: There were 18 oral lichen planus (OLP), 8 pemphigus vulgaris (PV), and 4 mucous membrane pemphigoid (MMP) patients with oral involvements. The mean age of the patients was 50.03 ± 9.71 years with a female predominance (male to female ratio 1:1.7). All patients suffered from chronic burning sensation and oral pain. DG was detected in all three mucocutaneous diseases but OLP was the main disease associated with DG (60%). However other oral mucosal lesions and extraoral involvements were more frequent in PV patients. Conclusions: According to these results, OLP, MMP, and PV are the mucocutaneous diseases responsible for DG, and OLP is the most common cause of DG. Clinicans should be familiar with the clinical presentations and signs (patient complaints) of DG-related mucocutaneous disease for early diagnosis, proper treatment, and quality of life of the patient

THE EFFECT OF DIODE LASER AS AN ADJUNCT TO PERIODONTAL TREATMENT ON CLINICAL PERIODONTAL PARAMETERS AND HALITOSIS: A RANDOMIZED CONTROLLED CLINICAL TRIAL

Aim: The aim of this study is to examine the clinical efficiency of diode laser periodontal pocket irradiation as an adjunct to conventional scaling and root planning (SRP) on periodontal parameters and halitosis. Material and Methods: In our randomized, controlled clinical trial, 40 patients with untreated chronic periodontitis were randomly separated into two group to receive SRP with laser (laser group n=20) or SRP solely (control group n=20). Plaque index (PI), gingival index (GI), probing pocket depth (PD), clinic attachment loss (CAL), bleeding on probing (BOP) and halitosis were recorded at baseline and 1st ,3rd and6th months after treatment by a periodontist. Results: Both treatment methods showed significant reductions in clinical parameters and halitosis levels compared to baseline. Conclusion: The present study indicates that compared to SRP solely, adjunctive applications of a 940-nm diode laser with SRP showed lower bleeding on probing and halitosis levels

Effect of sumac extract on serum oxidative status, RANKL/OPG system and alveolar bone loss in experimental periodontitis in rats

Objectives Sumac (Rhus coriaria L.) is widely used spice which has several properties such as antioxidant, anti-inflammatory and antimicrobial. The purpose of this animal study was to evaluate the effects of sumac extract on levels of receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG) expression, serum oxidative status, and alveolar bone loss in experimental periodontitis. Material and Methods Twenty-four Wistar rats were separated into three groups: non-ligated (NL, n=8), ligature only (LO, n=8), and ligature and treated with sumac extract (S, n=8) (20 mg/kg per day for 11 days). A 4/0 silk suture was placed around the mandibular right first molars subgingivally; after 11 days, the rats were sacrificed, and alveolar bone loss was histometrically measured. The detection of RANKL and OPG were immunohistochemically performed. Levels of serum total antioxidant status (TAS)/total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed. Results Alveolar bone loss was significantly greater in the LO group compared to the S and NL groups (p0.05). Conclusions The present study showed that systemic administration of sumac extract may reduce alveolar bone loss by affecting RANKL/OPG balance, TOS and OSI levels in periodontal disease in rats

The Effectiveness of Crataegus orientalis M Bieber. (Hawthorn) Extract Administration in Preventing Alveolar Bone Loss in Rats with Experimental Periodontitis.

The purpose of this animal study was to evaluate the effects of hawthorn (Crataeus orientalis M Bieber.) extract on serum oxidative status and alveolar bone loss in experimental periodontitis. Twenty-seven Wistar rats were assigned to one of the following groups: non- ligated+placebo (saline) (NL, n = 9), ligature only+placebo (saline) (LO, n = 9), and ligature and treated with hawthorn extract in saline (H, n = 9) (100 mg/kg orogastrically, once a day for 11 days). Periodontitis was induced by submerging a 4/0 silk ligature in the sulcus of the mandibular right first molars of rats, and the animals were sacrificed after 11 days. Micro-CT examinations were performed for linear and volumetric parameter assessment of alveolar bone. Periodontal tissues were histopathologically examined to assess the differences among the study groups. Levels of serum total antioxidant status (TAS)/total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed. Alveolar bone loss was significantly reduced by hawthorn administration compared to LO group (p<0.05). The number of inflammatory cells and osteoclasts in the LO group was significantly higher than that of the NL and H groups (p< 0.05). The number of osteoblasts in the LO and H groups was significantly higher than that of the NL group (p<0.05). TOS and OSI levels were significantly reduced in H group compared to LO group (P <0.05) and TAS levels were similar in H and NL group (p< 0.05). Hawthorn extract showed inhibitory effect on periodontal inflammation and alveolar bone loss by regulating TAS, TOS and OSI levels in periodontal disease in rats when administered systemically

Evaluation of temperature rise following the application of diode and ErCr:Ysgg lasers: an ex vivo study

Purpose Erbium, chromium: yttrium, scandium, gallium, garnet (ErCr:Ysgg) lasers have been frequently used in oral surgical procedures and are almost seen as alternatives to diode lasers. The aim of this comparative study was to analyze in an animal model the thermal elevation induced by ErCr:Ysgg and diode lasers in soft tissue and bone. Materials and methods Thirty freshly dissected sheep mandibles containing bone and soft tissue were divided into 120 equal parts. Gallium-aluminum-arsenide (Ga-Al-As) diode laser (λ=940 nm) with 1, 2 and 5 W output powers and ErCr:Ysgg laser (λ=2780 nm) with 2.75, 4.5 and 6 W output powers were used on soft and bone tissues separately for 3 seconds with point application. Mean temperature values before and after application of the lasers were compared in soft tissue and bone. Results The minimum mean temperature value was observed with 2.75 W ErCr:Ysgg laser while irradiation with 5 W diode laser created the maximum values (p<0.05). Conclusion ErCr:Ysgg laser (λ=2780 nm) with 2.75 W power generates low levels of heat compared to diode lasers and may provide safer surgery in soft and bone tissues without destructive effects of temperature increase

A- Mean ABL in study groups (mm).

<p><b>B</b>- Alveolar bone volume in study groups (mm³). * Significant difference compared to NL group. † Significant difference compared to LO.</p

The histological representive images of each experimental group.

<p>A- The histological images of NL group showing normal periodontium (red arrows: erythrocytes). <b>B</b>- LO group showing intense inflammatory cells (green arrow: lymphocyte, black arrow: neutrophils) and dilated blood vessels <b>C-</b> The histological images of H group showing moderate inflammatory cell infiltrate in periodontal ligament (Yellow arrows show inflammatory cells). (ab: alveolar bone, pdl: periodontal ligament).</p

A- Linear measurements are taken of ABL in the interdental space from the CEJ to ABC.

<p>(Green line: CEJ, red line: ABC, yellow line: Distance (mm)). <b>B-</b> Axially, a rectangular-shaped region of interest that is 0.33 mm far from each direction of tooth crown. Vertically, a rectangular-shaped region of interest include coronal halves of the slices through mesial root apex to the CEJ. C- Volumetric measurement with 3-D generated ROI.</p