Test diagnostique non invasif de morphométrie automatisée de l'histologie des polypes coliques

Test diagnostique non invasif de morphométrie automatisée de l’histologie des polypes colique

Autophagy induced by Helicobacter pylori infection is necessary for gastric cancer stem cell emergence

Background: The main cause of gastric cancer is the infection by the bacterium Helicobacter pylori which induces a chronic inflammation and an epithelial-to-mesenchymal transition (EMT) leading to the emergence of cells with cancer stem cell (CSC) properties. However, the underlying mechanisms have not been fully characterized. Moreover, H. pylori modulates the host cell autophagic process, but a few studies have investigated the role of this process in tumoral transformation. The aim of this study was to determine whether H. pylori-induced autophagy has a role in CSC emergence. Methods: Autophagic flux in response to H. pylori infection was characterized in AGS cell line expressing the tandem-tagged mCherry-GFP-LC3 protein and using a ratiometric flow cytometry analysis. Then, AGS and MKN45 cell lines were treated with bafilomycin or chloroquine, two pharmaceutical well-known inhibitors of autophagy, and different EMT and CSC characteristics were analyzed. Results: First, a co-expression of the gastric CSC marker CD44 and the autophagic marker LC3 in mice and human stomach tissues infected with H. pylori was observed. Then, we demonstrated in vitro that H. pylori was able to activate the autophagy process with a reduced autophagic flux. Finally, infected cells were treated with autophagy inhibitors, which reduced (i) appearance of mesenchymal phenotypes and migration ability related to EMT and (ii) CD44 expression as well as tumorsphere formation capacities reflecting CSC properties. Conclusion: In conclusion, all these data show that H. pylori-induced autophagy is implicated in gastric CSC emergence and could represent an interesting therapeutic target.This work was supported by the French foundation Ligue contre le Cancer (Pyrénées Atlantiques)

Bibliothèques et sciences de l\u27information : quel dialogue ? - Programme

Face aux évolutions technologiques, scientifiques, économiques, sociales, culturelles et politiques de leur environnement, les bibliothèques en tant qu\u27organismes culturels et scientifiques doivent repenser leurs pratiques, leur positionnement économique, politique et institutionnel, et leur rôle social, culturel et scientifique. Dans ce contexte, qu\u27attendent les bibliothèques de la recherche ? Quels thématiques et projets de recherche répondraient à leurs besoins ? Les sciences de l\u27information peuvent-elles apporter des réponses aux enjeux actuels ? Pour répondre aux interrogations posées par ces nouveaux défis, le colloque croise les approches et expériences de bibliothécaires et chercheurs en sciences de l\u27information de nombreux pays ( France, Canada, Etats-Unis, Allemagne, Royaume-Uni...

Progress with the Prime Focus Spectrograph for the Subaru Telescope: a massively multiplexed optical and near-infrared fiber spectrograph

The Prime Focus Spectrograph (PFS) is an optical/near-infrared multi-fiber spectrograph with 2394 science fibers, which are distributed in 1.3 degree diameter field of view at Subaru 8.2-meter telescope. The simultaneous wide wavelength coverage from 0.38 um to 1.26 um, with the resolving power of 3000, strengthens its ability to target three main survey programs: cosmology, Galactic archaeology, and galaxy/AGN evolution. A medium resolution mode with resolving power of 5000 for 0.71 um to 0.89 um also will be available by simply exchanging dispersers. PFS takes the role for the spectroscopic part of the Subaru Measurement of Images and Redshifts project, while Hyper Suprime-Cam works on the imaging part. To transform the telescope plus WFC focal ratio, a 3-mm thick broad-band coated glass-molded microlens is glued to each fiber tip. A higher transmission fiber is selected for the longest part of cable system, while one with a better FRD performance is selected for the fiber-positioner and fiber-slit components, given the more frequent fiber movements and tightly curved structure. Each Fiber positioner consists of two stages of piezo-electric rotary motors. Its engineering model has been produced and tested. Fiber positioning will be performed iteratively by taking an image of artificially back-illuminated fibers with the Metrology camera located in the Cassegrain container. The camera is carefully designed so that fiber position measurements are unaffected by small amounts of high special-frequency inaccuracies in WFC lens surface shapes. Target light carried through the fiber system reaches one of four identical fast-Schmidt spectrograph modules, each with three arms. Prototype VPH gratings have been optically tested. CCD production is complete, with standard fully-depleted CCDs for red arms and more-challenging thinner fully-depleted CCDs with blue-optimized coating for blue arms.Comment: 14 pages, 12 figures, submitted to "Ground-based and Airborne Instrumentation for Astronomy V, Suzanne K. Ramsay, Ian S. McLean, Hideki Takami, Editors, Proc. SPIE 9147 (2014)

Backed pieces and their significance in the Later Stone Age of the Horn of Africa

International audienc

Le CFEE : profond ancrage de la coopération scientifique franco-éthiopienne

Le Centre Français des études éthiopiennes (CFEE) est l’un des 27 Instituts français de recherche à l’étranger (IFRE) placés sous la double tutelle du Ministère de l’Europe et des Affaires étrangères (MEAE) et du CNRS. Il a pour vocation de soutenir des recherches menées en Éthiopie et dans la Corne de l’Afrique en sciences humaines et sociales et en sciences de l’environnement. Il est établi à Addis-Abeba, dans le quartier de Siddist Kilo, à proximité du Musée National d’Éthiopie et de l’Uni..

Archaeological survey around Lake Beseka (Northern Main Ethiopian Rift)

Here we report the recent archaeological survey we have conducted around Lake Beseka (Northern Main Ethiopian Rift) between 18th -24th December 2018. The survey is initiated, in the framework of the doctoral research of the first author (BH), to retrace the previous Later StoneAge sites, notably FeJx4, and to help document new archaeological context in the surroundings.FeJx4 is one of the earliest Later Stone Age sites in Ethiopia and the Horn which was excavated under the direction of Desmond Clark and Steven Brandt in 1970s (University of California, Berkeley). This survey work has thus brought renewed data on the archaeological and geological contexts of Lake Beseka area, more significantly by relocating the geological fault where the proverbial FeJx4 was excavated. Particularly, we are lucky enough to find this area remained unflooded considering the magnitude of the lake expansion since 1970s. Furthermore, the exploration around eastern Lake Beseka and Fentale areas has provided first-hand contextual information about the possible traces of prehistoric human occupations in the areas