The meteorologically driven circulation in mid-Chesapeake Bay

Utilizing 20 days of sea level elevation and current records in mid-Chesapeake Bay, the frequency structure of the meteorological forcing is investigated. For periods longer than 8 days the residual sea level inside the Bay is coupled to the coastal elevation forced by the onshore-offshore component of the wind, while at the 3–8 day scale the system was driven by the local longitudinal wind component. It is suggested, however, that the partition of energy between the longitudinal and lateral wind components may determine whether the Bay responds locally to the wind or to the coastal elevations. The 2–2.5 day sea level oscillations could not be identified with a seiche in the Bay; their source is not clear, but the atmospheric pressure is a possibility.The vertical structure of the residual currents through two cross-sections shows that the layers in the upper 8 m are directly driven by the wind, while in the deeper layers the flow is opposite the wind as a result of the surface slope associated with it. The lag in this counter flow increases from the bottom up and is explained in terms of the phase lag of the surface slope response to the wind and the depth dependence of the current response to the wind

Sedimentological characteristics of ice-wedge polygon terrain in Adventdalen (Svalbard): environmental and climatic implications for the late Holocene

Ice wedges are widespread periglacial features in the landscape of Adventdalen, Svalbard. The networks of ice wedges have created areas with well-developed polygonal terrains in the lowest fluvial terraces in this valley. We have examined the sedimentological characteristics of the northern and southern banks of the Advent river for palaeoenvironmental purposes. The base of two sedimentary sections reported radiocarbon dates of 3.3 and 3.9 ka BP, respectively. The northern site is constituted by three very different lithostratigraphical units, which suggests that their formation should be related to different environmental and climate conditions. By contrast, the southern section shows a rather homogeneous composition, with no significant variations in grain size and organic matter content. In both cases the uppermost sediments are constituted by a thick aeolian deposit. According to our data, warmer climate conditions may have prevailed during the mid Holocene until 3.3 ka BP with widespread peat formation in the valley bottom. Subsequently, a period with alternating soil formation and aeolian sedimentation took place from 3 to 2.5 ka BP, probably due to increasing climatic severity. During the last millennium a long-term cooling trend has favoured aeolian deposition in the lowest part of Adventdalen.info:eu-repo/semantics/publishedVersio

Determination of parathion in biological fluids by means of direct Solid Phase Microextraction.

A new and simple procedure for the determination of parathion in human whole blood and urine using direct immersion (DI) solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) is presented. This technique was developed using only 100 ìL of sample, and ethion was used as internal standard (IS). A 65-ìm Carbowax/divinylbenzene (CW/DVB) SPME fibre was selected for sampling, and the main parameters affecting the SPME process such as extraction temperature, adsorption and desorption time, salt addition, agitation and pH effect were optimized to enhance the sensitivity of the method. This optimization was also performed to allow the qualitative determination of parathion’s main metabolite, paraoxon, in blood. The limits of detection and quantitation for parathion were 3 and 10 ng/mL for urine and 25 and 50 ng/mL for blood, respectively. For paraoxon, the limit of detection was 50 ng/mL in blood. The method showed linearity between the LOQ and 50 ìg/mL for both matrices, with correlation coefficients ranging from 0.9954 to 0.9999. Precision and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The mean absolute recoveries were 35.1% for urine and 6.7% for blood. Other parameters such as dilution of sample and stability were also validated. Its simplicity and the fact that only 100 ìL of sample is required to accomplish the analysis make this method useful in forensic toxicology laboratories to determine this compound in intoxications, and it can be considered an alternative to other methods normally used for the determination of this compound in biological media

Governance with complex structures: evidence from Western European countries

This paper investigates if the existence of complex structures plays an important role in corporate governance. It uses GMM estimation on a panel of Western European firms. We find that the presence of a second and third large shareholder has a significant positive effect on firm value. This study underlines the importance of the number of blockholders as a determinant of firm value, when taken as a moderator of the contestability effect. It shows that the legal context and company-specific characteristics play a crucial moderating role for contestability. In contrast to previous research, we find that contestability plays a less relevant role in family firms. We also find that this last result does not vary significantly with the identity of the remaining elements of the coalition. Also, our study suggests that contestability is less important in companies led by majority shareholders

Obtaining triplet-triplet absorption spectra and triplet lifetimes of long-lived molecules with a UV-Visible spectrophotometer

A simple method for obtaining triplet-triplet absorption (TTA) spectra and triplet state (T1) lifetimes of long-lived triplets is presented and demonstrated with the polycyclic aromatic hydrocarbon coronene (both normal and perdeuterated forms) in a polymer matrix at room temperature. The TTA spectra obtained with a camera flash and a spectrophotometer are noisier but otherwise identical to those obtained with a state-of-the-art flash photolysis apparatus. The triplet lifetimes obtained from transient absorption are identical to the phosphorescence lifetimes of the same samples.This article is based upon work from COST Action CA21126 - Carbon molecular nanostructures in space (NanoSpace), supported by COST (European Cooperation in Science and Technology), and by projects iBB (UIDB/04565/2020 and UIDP/04565/2020) and i4HB (LA/P/0140/2020), Fundação para a Ciência e a Tecnologia (FCT), Portugal

Identification of cell-surface mannans in a virulent Helicobacter pylori strain

With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: (image) In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host–microbial interactions and immunological modulation.This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) through project Pylori E&LPS POCI/QUI/56393/2004, PhD grant SFRH/BD/19929/2004, by the Natural Sciences and Engineering Research Council of Canada (NSERC), and by the European Network of Research Infrastructures within the 6th Framework Programme of the EC (Contract # RII3-026145, EU-NMR). The authors further thank Dr. Adrien Favier (RALF-NMR facility, Grenoble - France) for conducting NMR experiments

Opinião dos encarregados de educação relativamente à dinâmica das aulas de natação para bebés no distrito de Setúbal

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Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens

Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori clarithromycin resistance. In the retrospective study (n=30 patients), full agreement between PNA-FISH and PCR-sequencing was observed. Compared to the reference method (culture followed by Etest), the specificity and sensitivity of PNA-FISH were 90.9% (95% confidence interval [CI], 57.1% to 99.5%) and 84.2% (95% CI, 59.5% to 95.8%), respectively. In the prospective cohort (n=93 patients), 21 cases were positive by culture. For the patients harboring clarithromycin- resistant H. pylori, the method showed sensitivity of 80.0% (95% CI, 29.9% to 98.9%) and specificity of 93.8% (95% CI, 67.7% to 99.7%). These values likely represent underestimations, as some of the discrepant results corresponded to patients infected by more than one strain. PNA-FISH appears to be a simple, quick, and accurate method for detecting H. pylori clarithromycin resistance in paraffin-embedded biopsy specimens. It is also the only one of the methods assessed here that allows direct and specific visualization of this microorganism within the biopsy specimens, a characteristic that allowed the observation that cells of different H. pylori strains can subsist in very close proximity in the stomach. Copyright © 2013, American Society for Microbiology. All Rights Reserved.This work was supported by the Portuguese Institute Fundacao para a Ciencia e a Tecnologia (Ph.D. grant SFRH/BD/38124/2007 and project PIC/IC/82815/2007)