Low-Cost Motility Tracking System (LOCOMOTIS) for time-lapse microscopy applications and cell visualisation

This article has been made available through the Brunel Open Access Publishing Fund.Direct visualisation of cells for the purpose of studying their motility has typically required expensive microscopy equipment. However, recent advances in digital sensors mean that it is now possible to image cells for a fraction of the price of a standard microscope. Along with low-cost imaging there has also been a large increase in the availability of high quality, open-source analysis programs. In this study we describe the development and performance of an expandable cell motility system employing inexpensive, commercially available digital USB microscopes to image various cell types using time-lapse and perform tracking assays in proof-of-concept experiments. With this system we were able to measure and record three separate assays simultaneously on one personal computer using identical microscopes, and obtained tracking results comparable in quality to those from other studies that used standard, more expensive, equipment. The microscopes used in our system were capable of a maximum magnification of 413.6x. Although resolution was lower than that of a standard inverted microscope we found this difference to be indistinguishable at the magnification chosen for cell tracking experiments (206.8x). In preliminary cell culture experiments using our system, velocities (mean mm/min ± SE) of 0.81±0.01 (Biomphalaria glabrata hemocytes on uncoated plates), 1.17±0.004 (MDA-MB-231 breast cancer cells), 1.24±0.006 (SC5 mouse Sertoli cells) and 2.21±0.01 (B. glabrata hemocytes on Poly-L-Lysine coated plates), were measured and are consistent with previous reports. We believe that this system, coupled with open-source analysis software, demonstrates that higher throughput time-lapse imaging of cells for the purpose of studying motility can be an affordable option for all researchers. © 2014 Lynch et al

Three-axis adjustable loading structure

A three axis adjustable loading structure for testing the movable surfaces of aircraft by applying pressure, is described. The device has three electric drives where the wall angle, horizontal position, and vertical position of the test device can be rapidly and accurately positioned

High-tip-speed fiber composite compressor blades: Vibration and strength analysis

An analytical procedure is described which couples composite mechanics computer codes with NASTRAN. This procedure was used to perform a detailed analysis of a high-tip-speed fiber composite compressor fan blade. The results indicate that the various vibration modes of this blade are highly coupled. Mechanical load ply stresses are well below the corresponding room temperature strengths. Lamination residual stresses are likely to cause transply cracks and interply delamination. Transply cracks and relaxation of root fixity decrease the vibrational frequencies whereas centrifugal stiffening increases them. Comparisons of results for various parameters are presented in tabular and graphical form

Analysis of stresses at the bore of a drilled ball operating in a high-speed bearing

Three-dimensional stress distributions were calculated for both a regular drilled ball with a stiffening web. The balls were 20.6 mm (0.8125 in.) in diameter and had a 12.6 mm (0.496 in.) diameter concentric hole. The stiffening web was 1.5 mm (0.06 in.) thick. The calculations showed that a large reversing tangential stress at the hole bore was reduced by one-half by the addition of the web

Aerosol studies in mid-latitude coastal environments in Australia

The results of the evaluation of several inversion procedures that were used to select one which provides the most accurate atmospheric extinction profiles for small aerosol extinction coefficients (that often predominate in the maritime airmass) are presented. Height profiles of atmospheric extinction calculated by a two component atmospheric solution to the LIDAR equation will be compared with corresponding in-situ extinction profiles based on the size distribution profiles obtained in Western Australia. Values of the aerosol backscatter to extinction ratio obtained from multi-angle LIDAR measurements will be used in this solution

Magnetic-Island Contraction and Particle Acceleration in Simulated Eruptive Solar Flares

The mechanism that accelerates particles to the energies required to produce the observed high-energy impulsive emission in solar flares is not well understood. Drake et al. (2006) proposed a mechanism for accelerating electrons in contracting magnetic islands formed by kinetic reconnection in multi-layered current sheets. We apply these ideas to sunward-moving flux ropes (2.5D magnetic islands) formed during fast reconnection in a simulated eruptive flare. A simple analytic model is used to calculate the energy gain of particles orbiting the field lines of the contracting magnetic islands in our ultrahigh-resolution 2.5D numerical simulation. We find that the estimated energy gains in a single island range up to a factor of five. This is higher than that found by Drake et al. for islands in the terrestrial magnetosphere and at the heliopause, due to strong plasma compression that occurs at the flare current sheet. In order to increase their energy by two orders of magnitude and plausibly account for the observed high-energy flare emission, the electrons must visit multiple contracting islands. This mechanism should produce sporadic emission because island formation is intermittent. Moreover, a large number of particles could be accelerated in each magnetohydrodynamic-scale island, which may explain the inferred rates of energetic-electron production in flares. We conclude that island contraction in the flare current sheet is a promising candidate for electron acceleration in solar eruptions.Comment: Accepted for publication in The Astrophysical Journal (2016

Extracellular Phosphodiesterase from the Growth Medium of the Myxomycete Physarum flavicomum

The plasmodium of the myxomycete Physarum flavicomum secretes cyclic AMP phosphodiesterase into the medium. The extracellular enzyme had a pH optimum between 7 and 8 and a Km of about 500 μM cyclic AMP and was inhibited by theophylline, caffeine and 3-isobutyl-l-methyxanthine (MIX). A marked decrease of enzyme activity was noted in the presence of EDTA, suggesting the requirement of Mg+ + by the enzyme. Addition of Mg+ + and Ca + + stimulated the enzyme while Zn+ + , Co+ + , Pb+ + , Mn+ + , Fe + + + , Ni+ + , and Cu + + all inhibited phosphodiesterase activity. An interesting feature of this extracellular phosphodiesterase was its ability to retain full catalytic activity after prolonged exposure to elevated temperature

Analysis of the JPlan Exercise as an Experimental Learning tool

The purpose of this study is to develop an effective educational tool for the Combat Logistics course offered at the Air Force Institute of Technology. The current tool being used, the JPLAN Exercise, was identified as outdated in several areas. The research focused upon four investigative questions what makes an effective educational tool, should the existing exercise be revised or replaced, which logistics principles are essential for incorporation into the educational tool, and is the updated tool significantly different from the original. To answer these questions, we conducted an extensive literature review that focused on two areas. The first area was concerned with accepted educational methods. The second area was concerned with logistics lessons learned from major US conflicts since World War I, as well as current military logistics issues. This information was used to develop a revised JPLAN Exercise. This revised exercise was given to students along with the original exercise. We surveyed the students about differences in value, currency, and realism between the two exercises, as well as their perceived self-efficacy following exposure to each. Recommendations were to begin using the revised JPLAN Exercise in future course offerings, as well as to perform further research

Low-energy interband absorption in bcc Fe and hcp Co

We have examined the electronic structure of bcc Fe and single-crystal hcp Co by using optical absorptivity and thermoreflectance techniques for 0.2≤hν≤5 eV. The optical conductivities σ were calculated by Kramers-Kronig analyses. A prominent structure was observed in σ for Fe at 2.37 eV and a shoulder was observed near 0.8 eV; the latter structure was the dominant feature in the thermoreflectance spectrum. These were discussed in terms of minority-spin band interband absorption and spin-flip interband transitions. The anisotropic optical conductivities of hcp Co were discussed in terms of recent energy-band calculations

Extended radio emission associated with a breakout eruption from the back side of the Sun

Context. Coronal mass ejections (CMEs) on the Sun are the largest explosions in the Solar System that can drive powerful plasma shocks. The eruptions, shocks, and other processes associated to CMEs are efficient particle accelerators and the accelerated electrons in particular can produce radio bursts through the plasma emission mechanism. Aims. Coronal mass ejections and associated radio bursts have been well studied in cases where the CME originates close to the solar limb or within the frontside disc. Here, we study the radio emission associated with a CME eruption on the back side of the Sun on 22 July 2012. Methods. Using radio imaging from the Nancay Radioheliograph, spectroscopic data from the Nancay Decametric Array, and extreme-ultraviolet observations from the Solar Dynamics Observatory and Solar Terrestrial Relations Observatory spacecraft, we determine the nature of the observed radio emission as well as the location and propagation of the CME. Results. We show that the observed low-intensity radio emission corresponds to a type II radio burst or a short-duration type IV radio burst associated with a CME eruption due to breakout reconnection on the back side of the Sun, as suggested by the pre-eruptive magnetic field configuration. The radio emission consists of a large, extended structure, initially located ahead of the CME, that corresponds to various electron acceleration locations. Conclusions. The observations presented here are consistent with the breakout model of CME eruptions. The extended radio emission coincides with the location of the current sheet and quasi-separatrix boundary of the CME flux and the overlying helmet streamer and also with that of a large shock expected to form ahead of the CME in this configuration.Peer reviewe