Effect of Aspirin on Cell Growth of Human MG-63 Osteosarcoma Line

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in bone tissue repair treatment for their pharmacological action. The objective of this study was to determine the effect of aspirin, on osteoblast growth, using MG63 cell line as osteoblast model. MTT spectrophotometry results showed that 20, 100, and 1000 μM aspirin doses have an inhibitory effect on growth. Cell cycle analysis revealed that aspirin doses of 100 and 1000 μM arrest the cell cycle in phase GO/G1. Parallel apoptosis/necrosis studies showed no changes in comparison to control cells after treatment with 1 or 10 μM aspirin but a significantly increased percentage of cells in apoptosis at doses of 20, 100, and 1000 μM. We highlight that treatment of osteoblast-like cells with 1000 μM aspirin increased not only the percentage of cells in apoptosis but also the percentage of necrotic cells, which was not observed in aspirin treatments at lower doses

Impact of parental involvement on the communication skills of children with autism

In pursuit of every individual’s access to education regardless of one’s statusin life, the Philippines establishedspecial education(SPED) centers to caterto children with special needs.Children with autism are the expectedbeneficiaries of these centers. This research assessed the impact of parentalinvolvement on the communication skills of children with autism at a SPEDcenter in Cebu City, Cebu, Philippines. Their teachers, regardingtheircommunication skills, assessed thirty children with autism. At the sametime, parents were asked to rate their extent of involvement in their child’sschool activities using survey questionnaires. Different statistical tools wereused to treat the data gathered, such as the weighted mean, standarddeviation, andPearsonproduct moment correlation coefficient. Resultsrevealed that parents were involved in the school activities of their children.However, the children had a moderate manifestation of communicationskills. It was found that there is a moderate positive significant relationshipbetween the parents’involvement and the communication skills of thechildren. Thus, teachers are encouraged to design programs for the childrenthat would involvethe parents in enhancing the communication skills ofchildren with autism

An RNA Polymerase III General Transcription Factor Engages in Cell Type-Specific Chromatin Looping

Transcription factors (TFs) bind DNA in a sequence-specific manner and are generally cell type-specific factors and/or developmental master regulators. In contrast, general TFs (GTFs) are part of very large protein complexes and serve for RNA polymerases’ recruitment to promoter sequences, generally in a cell type-independent manner. Whereas, several TFs have been proven to serve as anchors for the 3D genome organization, the role of GTFs in genome architecture have not been carefully explored. Here, we used ChIP-seq and Hi-C data to depict the role of TFIIIC, one of the RNA polymerase III GTFs, in 3D genome organization. We find that TFIIIC genome occupancy mainly occurs at specific regions, which largely correspond to Alu elements; other characteristic classes of repetitive elements (REs) such as MIR, FLAM-C and ALR/alpha are also found depending on the cell’s developmental origin. The analysis also shows that TFIIIC-enriched regions are involved in cell type-specific DNA looping, which does not depend on colocalization with the master architectural protein CTCF. This work extends previous knowledge on the role of TFIIIC as a bona fide genome organizer whose action participates in cell type-dependent 3D genome looping via binding to REs

Cooling Strategies for Greenhouses in Summer: Control of Fogging by Pulse Width Modulation

The possibilities for improving the control of greenhouse fogging systems, were studied by comparing several combinations of ventilation cooling techniques, shade screening and low-pressure fogging. The study was divided into three parts: experiments, modelling and simulations. In the first part of the paper, ten combinations of five cooling techniques were tested during the summers of 2002 and 2003 in a 132m2 greenhouse with a steel structure and a single-layer methacrylate cover located in Madrid, Spain. An analysis of variance of the climatic parameters was carried out to determine which combinations produced significant differences in inside temperature or relative humidity. Comparing the values for the inside to outside temperature difference, the combination of a shade screen and above-screen fogging achieved a difference in temperature almost the same as that for under-screen fogging, but the relative humidity was significantly lower. In the second part of the study a dynamic model was developed (2002) and validated (2003). The mean absolute error obtained for inside temperature was similar in the fit and the validation and it was less than 1.5 1C in both cases. The model was used to simulate the inside air temperature for a fog system working without shading, and above and under a shade screen. Control algorithms were developed for reducing system water consumption. In the three cases a simple on/off control with a fixed fogging cycle was compared with a pulse width modulation (PWM) strategy, in which the duration of the fogging pulse was increased as a function of inside temperature. The strategies with PWM applied to the fog system were able to reduce water consumption by 8–15% with respect to the strategies with a fixed fogging cycle

Study of idfferential protein expression in healthy human skeletal muscles using electrophoresis bidimentional

Comunicaciones a congreso

Epidemiología de las fracturas de la extremidad proximal del fémur en asturias

Hemos realizado un estudio epidemiológico de las fracturas de fémur proximal en personas mayores de 50 años, atendidas en nuestro Hospital durante los años 1990-92. Se valoraron un total de 355 casos. En el período estudiado se observó que las fracturas más numerosas fueron la cervicales, no encontrando diferencias significativas en los ingresos según el período estacional. La edad media de presentación fue de 81 años. La tasa de incidencia fue muy semejante a las otras provincias españolas y menor que la encontrada en los países del Norte de Europa, Inglaterra y Estados Unidos.We have performed an epidemiologic study on the incidence of proximal femoral fractures in patients older than 50 years, all reviewed in our Hospital from 1990 to 1992. We included 355 patients. Femoral neek fractures were the most frequent lesions. No significant differences in the hospital admissions were found during all the year's seasons. The mean age of presentation was 81 years. The incidence rate was similar to the rest of Spain, and less than in North Europe, England and United States

Fungos associados a sementes de quinoa (Chenopodium quinoa wield).

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Universal restrictions to the conversion of heat into work derived from the analysis of the Nernst theorem as a uniform limit

We revisit the relationship between the Nernst theorem and the Kelvin-Planck statement of the second law. We propose that the exchange of entropy uniformly vanishes as the temperature goes to zero. The analysis of this assumption shows that is equivalent to the fact that the compensation of a Carnot engine scales with the absorbed heat so that the Nernst theorem should be embedded in the statement of the second law. ----- Se analiza la relaci{\'o}n entre el teorema de Nernst y el enunciado de Kelvin-Planck del segundo principio de la termodin{\'a}mica. Se{\~n}alamos el hecho de que el cambio de entrop{\'\i}a tiende uniformemente a cero cuando la temperatura tiende a cero. El an{\'a}lisis de esta hip{\'o}tesis muestra que es equivalente al hecho de que la compensaci{\'o}n de una m{\'a}quina de Carnot escala con el calor absorbido del foco caliente, de forma que el teorema de Nernst puede derivarse del enunciado del segundo principio.Comment: 8pp, 4 ff. Original in english. Also available translation into spanish. Twocolumn format. RevTe

Más allá de las dimensiones del cuidado. Sistematización de la experiencia de enseñanza aprendizaje sobre el cuidado

The systematization of the teaching experience in human care is part of the project called Human Care in Nursing Education, Research, Care, and Administration. Its objective is to encourage the sharing of experiences in teaching and learning about Human Care, in order to make known the reach of the interaction and to recognize the knowledge that has been promoted or where a synergy has been generated from practice, collective and personal experience. The design of the study is qualitative; using the Participative Action Research and Learning by Experience methods. In this study, we describe what we do, feel and live, documenting the participants' opinion regarding, what is care? From the interpretation of opinion emerges a conceptual synergy unique, its own, and novel regarding the definition of care given by the respondents in a collective teaching session. Caring is to give love and support to a person in order to minimize the anguish and enter into a stage of tranquility, providing a feeling of security and confidence at the moment. (Affection 1): Caring is direct or indirect attention that is given to the human being founded on holism, observation, communication and feelings in order to strengthen the weaknesses and team work and, in this way, achieves what has been planned. (Affection 5) and among others, human care is the key to caring with human quality founded in affection, confidence and personal presence with the objective that the person achieves the greatest satisfaction in the moment and occasion of his/her health status. (Affection 24).Esta sistematización de experiencia sobre la enseñanza del Cuidado Humano se inscribe en el proyecto titulado Cuidado Humano en Educación, Investigación, Administración y Cuidado en Enfermería, cuya finalidad es favorecer el intercambio de experiencias en la enseñanza y el aprendizaje sobre el Cuidado Humano; concienciar los alcances de la interacción y reconocer el conocimiento que se ha potenciado o dónde se ha generado una sinergia desde la práctica, la experiencia colectiva y la experiencia personal. Materiales y Métodos: El diseño del estudio es cualitativo; utilizando el método Investigación Acción Participativa y el Aprendizaje por Experiencia. En el mismo se describe lo que hacemos, sentimos y vivimos, documentando la opinión de los participantes sobre ¿Qué es el cuidado? De la interpretación de las opiniones, emerge una sinergia conceptual, única, propia y novedosa sobre la definición de cuidado dada por los informantes, en una sesión colectiva de enseñanza. Los resultados son los conceptos de cuidado que emergen de forma empírica y significativa: entre otros, Cuidado es brindar amor y apoyo a la persona para que minimice la angustia y entre en un estadio de tranquilidad, proporcionándole en el momento, un sentimiento de seguridad y confianza. (Afecto 1); y entre otros, el cuidado humano es la clave de la atención, con calidad humana, fundamentada en el afecto, la confianza y con la presencia del personal, con el fin de que la persona alcance la mayor satisfacción, en el momento y en la ocasión, de su estado de salud. (Afecto 24). En conclusión, la sistematización de la práctica pedagógica sobre el cuidado humano proporciona una de las vías para hacer más significativo entre las enfermeras la contribución del cuidado en la vida de los cuidados y de los cuidadores como principio universal, conjuntamente, con el amor, en la vida de los humanos y de la humanidad