530 research outputs found

    Let’s talk about sex: female therapists’ experience of working with male clients who are sexually attracted to them

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    Sexual attraction in psychotherapy is an under researched area of psychotherapeutic practice. Literature suggests that the direction of the work with sexual attraction is determined by the therapist’s emotional reaction to it which can either halt or spur its exploration with the client and occasionally cause serious ethical misconduct such as sexual acting out and premature therapeutic termination. Given the suggestion that our socio-cultural context is particularly prohibitive of the exploration of the male client’s erotic feelings towards his female therapist, this study focused on female therapists, exploring the nature of their experience working with male clients who are sexually attracted to them and the extent to which they used the experience in their work. The study used Hollway and Jefferson’s (2008) hybrid method ‘Free association narrative interview’. This involved multiple unstructured interviews with five female participants. Follow up interviews provided opportunities for building trust, consideration of non-conscious communication and permitted collaborative meaning-making with participants as co-researchers, considered particularly important given the ‘taboo’ status of the topic. Each participant’s data was initially analysed on its own merit after which data was compared across participants. The results of the study included participants’ emphasis on the uniqueness of working with each different client, the importance of confidentiality, trust and supervision in the work and the link between issues of sexual attraction and our identity. Participants’ experience differed depending on whether they felt reciprocally towards their clients or not. On occasions when therapists were not attracted towards their clients, they expressed a sense of vulnerability, discomfort with power inequality and a sense of being at fault. Conversely, when therapists felt sexually attracted to their clients, they experienced a sense of the splitting of the roles of being a woman and a therapist, as well as the experience of love and mutuality in relation to clients. In terms of the therapists’ use of the experience in their work, the ability to do so was reported within both categories of responses, albeit it seemed that this was easier done in the context of a therapeutic relationship which was described as ‘mutual and loving’. The implications of these results are manifold. In practical terms, my research suggests a need for therapists to consider the safety of the location at which they work. In relation to therapy training, the findings suggest that working with sexual dynamics is not something which can be learnt and mastered but instead needs to be explored reflectively and reflexively. From the clinical point of view, this research suggests that sexual dynamics can be experienced as merging the gap between ‘personal’ and ‘professional’ parts of therapists’ identity because of which the role of personal therapy and clinical supervision are highlighted as particularly important. Finally, my study considered the impact of power inequality on the therapists’ sense of authority from the clinical perspective as well as the context of wider societal dynamics and offered links with previously published research, suggesting future areas for research and clinical practice

    Endocrine Disruption by Heavy Metals on Steroidogenesis in Model Systems

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    In this study human adrenocortical carcinoma cell line NCI-H295R was used as an in vitro biological model to study the effect of heavy metals on steroidogenesis. The cell cultures were exposed to different concentrations of cadmium (1.90; 3.90; 7.80; 15.60; 31.20; 62.50 μM of CdCl2), mercury (1.0; 5.0; 25; 50; 100 μM of HgCl2), nickel (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250; 500 μM of NiCl2) and compared to control. Cell viability was measured by the metabolic activity (MTT) assay for estimation of mitochondria structural integrity. Quantification of sexual steroid production directly from aliquots of the medium was performed by enzyme linked immunosorbent assay (ELISA). Cadmium decreased the release of progesterone and testosterone already at a very low concentration (1.90 μM) of CdCl2, while the cell viability remained relatively high (> 75%) up to 7.80 μM of CdCl2 and significantly (P<0.01) decreased at 15.60 μM and higher concentrations of CdCl2. Concentration-dependent depression in testosterone production was detected particularly for higher concentration of HgCl2. Progesterone production was also decreased, but at the lower concentrations (1.0 and 5.0 μM) of HgCl2 this decline was lower compared to depression of testosterone. The cell viability significantly decreased at 25 μM and higher concentration of HgCl2. Results of the our study indicate dose dependent decreases in both sexual steroid hormones by NCI-H295R cell culture following a 48 h in vitro NiCl2 exposure. The lowest concentration of progesterone was significantly (P<0.01) detected in groups with the higher doses (≥ 500 μM) of NiCl2, which elicited significant cytotoxic effect. The testosterone production was decreased as well, but this decline was more pronounced compared to depression of progesterone. These results suggest that heavy metals have detrimental effects on steroid hormone synthesis even at very low concentrations and consecutively on reproductive physiology

    Comparison of semen characteristics and histological structure of the testis from transgenic and non-transgenic rabbits

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    [EN] The aim of this study was to compare semen characteristics including sperm quantity, quality, and abnormalities, as well as histological structure of the testis of three-year old transgenic (human clotting factor, hFVIII, gene) and nontransgenic rabbits. For the experiment, 10 transgenic rabbits of F2 and F3 generations and 10 randomly selected non-transgenic males of the same breed and age were used as controls. All males were housed in individual cages, under a the same environmental conditions: photoperiod (14L:10D), temperature (18-20°C), and humidity (65-70%). Semen samples, collected once a week for 20 wk from each control and transgenic male, were analyzed by computer assited semen analysis within a few minutes following natural ejaculation into an artificial vagina. Concentration of spermatozoa was higher in the transgenic than in the non-transgenic group (P<0.001; 316.6±148.8 and 126.7±64.4¿106/ mL, respectively). Significant differences (P<0.1) between transgenic and non-transgenic males were observed also in spermatozoa motility (63.08 vs. 32.60%). Significantly higher (P<0.05) relative volume (8.08±2.89%) and diameter of testicular lumen (36.89±23.11 ¿m) were found in the transgenic animals compared to control animals (16.69±4.70%, 53.89±25.42 ¿m). Our results show that spermatozoa parameters and histological structure of the testis can be used for the characterization of male reproductive traits of older transgenic rabbits.This work was supported by the grant No: 2003 SP51/028 09 00/028 09 03 coordinated by the Slovak Academy of Science and by the grant of Ministry of Agriculture Slovak Republic (RÚVV 07-012). All experiments were approved according to ethical permission No. SK P 28004. We are grateful to Dr. Shoubadeep Roychoudhury for the English correction.Lukac, N.; Massanyi, P.; Flesarova, S.; Danko, J.; Makarevich, A.; Chrenek, P. (2009). Comparison of semen characteristics and histological structure of the testis from transgenic and non-transgenic rabbits. World Rabbit Science. 17(4):221-226. https://doi.org/10.4995/wrs.2009.64722122617

    Defects in High Entropy Alloy HfNbTaTiZr Prepared by High Pressure Torsion

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    High entropy alloy HfNbTaTiZr was successfully processed by severe plastic deformation using high pressure torsion (HPT) and ultrafine grained microstructure was achieved. The microstructure of HPT-deformed HfNbTaTiZr alloy was characterized by X-ray diffraction and compared with conventionally cast ingots. The lattice defects introduced by HPT processing were characterized by positron annihilation spectroscopy. The X-ray diffraction profiles of HTP-deformed samples were extremely broadened due to small sizes of coherently diffracting domains and a high microstrain introduced by severe plastic deformation.11Ysciescopu
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