Efficacy of electrical Russian current stimulation after end-to-side neurorrhaphy of the common fibular nerve: electroneuromyography and muscle strength analysis

Peripheral nerve injury leads to a high functional loss of muscle tissue. Thus, many studies have investigated surgical techniques, such as neurorraphies, and therapeutic resources, such as electrical stimulation, to improve the functionality of reinnervated muscle after peripheral injury. This study aims to investigate the effects of electrical stimulation with Russian Current (2,500Hz, 4ms, 10:20 sec contraction/relaxation, modulated at 10Hz and 100Hz) in the functional recovery after section and end-to-side neurorrhaphy of the peroneal nerve distal stump common to the lateral face of the tibial nerve in rats. In this study, 25 male Wistar rats with 80 days of life were used, provided by the Universidade Sagrado Coração (USC), Bauru, SP, Brazil. The animals were randomly divided into five groups: Initial Control Group (ICG), Final Control Group (FCG), Untreated Experimental Group (UEG), Endto-Side Neurorrhaphy with Russian Stimulation Group (ENRSG), and Denervated Control Group (DCG). The Russian Current was started 5 days after neurorrhaphy and applied to the cranial tibial muscle of the ENRSG, 3 times a week, totaling 36 sessions. We observed that the electrical stimulation with Russian Current (ENRSG) was effective to increase amplitude (mV) and to decrease the latency (ms) of the reinnervated muscle, besides increasing the muscle strength when compared with the denervated control group. Therefore, we concluded that the average frequency electrical stimulation (Russian current) was efficient in the functional recovery of the cranial tibial muscle after the end-lateral neurorrhaphy of the common fibular nerve.Lesões de nervos periféricos levam a perda funcional elevada no tecido muscular. Assim, muitas pesquisas têm investigado técnicas cirúrgicas, como neurorrafias, e recursos terapêuticos, como eletroestimulação, para melhorar a funcionalidade de um músculo reinervado após lesão periférica. Este estudo tem como objetivo investigar os efeitos da eletroestimulação com corrente russa (2.500Hz, 4ms, 10 seg. de contração por 20 seg. de relaxamento, modulação de 10Hz e 100 Hz) na recuperação funcional após secção e neurorrafia término-lateral do coto distal do nervo fibular comum à face lateral do nervo tibial em ratos. Foram utilizados 25 ratos Wistar, machos, com 80 dias de vida, fornecidos pelo Biotério Central da Universidade Sagrado Coração (Bauru, SP, Brasil). Os animais foram divididos aleatoriamente em cinco grupos: grupo-controle Inicial (GCI), grupo-controle final (GCF), grupo experimental não tratado (GENT), grupo neurorrafia término-lateral com estimulação russa (GNTLER) e grupocontrole desnervado (GCD). A corrente russa foi iniciada cinco dias após neurorrafia e aplicada no músculo tibial cranial do GNTLER, 3 vezes por semana, totalizando 36 sessões. A estimulação elétrica foi eficaz para aumentar a amplitude e diminuir a latência do músculo reinervado, além de aumentar a força muscular em comparação ao GCD. Diante disso, conclui-se que a eletroestimulação de média frequência (corrente russa) foi eficiente na recuperação funcional do músculo tibial cranial após neurorrafia término-lateral do nervo fibular comum.Las lesiones de los nervios periféricos ocasionan una elevada pérdida funcional en el tejido muscular. De esta manera, en muchos estudios se han investigado técnicas quirúrgicas, como neurorrafias, y recursos terapéuticos, como la electroestimulación, para mejorar la funcionalidad del músculo reinervado tras una lesión periférica. El presente estudio tiene como objetivo investigar los efectos de la electroestimulación con corrente rusa (2.500Hz, 4ms, 10 seg. de contracción por 20 seg. de relajación, modulación de 10Hz y 100Hz) en la recuperación funcional tras la sección y neurorrafia términolateral del muñón distal del nervio fibular común en la parte lateral del nervio tibial en ratas. Se utilizaron 25 ratas Wistar, machos, con 80 días de vida, proporcionadas por el Biotério Central de la Universidade do Sagrado Coração (Bauru, SP, Brasil). Se dividieron aleatoriamente los animales en cinco grupos: grupo de control inicial (GCI), grupo de control final (GCF), grupo experimental no tratado (GENT), grupo de neurorrafia término-lateral con estimulación rusa (GNTLER) y grupo de control denervado (GCD). La corriente rusa se inició cinco días tras la neurorrafia, siendo que la aplicó al músculo tibial craneal del GNTLER 3 veces a la semana, con un total de 36 sesiones. La estimulación eléctrica se mostró efectiva para aumentar la amplitud y disminuir la latencia del músculo reinervado, además de aumentar la fuerza muscular en comparación con el GCD. Por lo tanto, se concluye que la estimulación eléctrica de frecuencia media (corriente rusa) fue eficaz en la recuperación funcional del músculo tibial craneal tras la neurorrafia término-lateral del nervio fibular común

Using Chatbot Technologies to Support Argumentation

Chatbots are extensively used in modern times and are exhibiting increasingly intelligent behaviors. However, being relatively new technologies, there are significant demands for further advancement. Numerous possibilities for research exist to refine these technologies, including integration with other technologies, especially in the field of artificial intelligence (AI), which has received much attention and development. This study aims to explore the ability of chatbot technologies to classify arguments according to the reasoning patterns used to create them. As argumentation is a significant aspect of human intelligence, categorizing arguments according to various argumentation schemes (reasoning patterns) is a crucial step towards developing sophisticated human-computer interaction interfaces. This will enable agents (chatbots) to engage in more sophisticated interactions, such as argumentation processes

Introducing Bode: A Fine-Tuned Large Language Model for Portuguese Prompt-Based Task

Large Language Models (LLMs) are increasingly bringing advances to Natural Language Processing. However, low-resource languages, those lacking extensive prominence in datasets for various NLP tasks, or where existing datasets are not as substantial, such as Portuguese, already obtain several benefits from LLMs, but not to the same extent. LLMs trained on multilingual datasets normally struggle to respond to prompts in Portuguese satisfactorily, presenting, for example, code switching in their responses. This work proposes a fine-tuned LLaMA 2-based model for Portuguese prompts named Bode in two versions: 7B and 13B. We evaluate the performance of this model in classification tasks using the zero-shot approach with in-context learning, and compare it with other LLMs. Our main contribution is to bring an LLM with satisfactory results in the Portuguese language, as well as to provide a model that is free for research or commercial purposes.Comment: 10 pages, 3 figure

A simplified curcumin targets the membrane of Bacillus subtilis

Abstract Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti‐inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram‐positive bacterium Bacillus subtilis. The anti‐B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP‐dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β‐diketone moiety, showed antibacterial action against gram‐positive and gram‐negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra‐cellular localization. Finally, we show here experimental evidence for the requirement of the β‐diketone group of curcumin for its interaction with FtsZ

Os e-sports e os Jogos Olímpicos: Perspectivas de um debate em andamento

This literature review paper aims to understand the different perspectives on the inclusion or not of eSports in the Olympic Games programme. ESports (electronic sports) popularity among world’s youth population grows daily. The eSports phenomenon became a new form of entertainment with its own financial means and cultural practices, very close related to streaming technology, and ultimately bringing new questions and challenges for the so-called ‘traditional sports’ field. The audience success sparked the gaming industry with a considerable economic growth, transforming a backyard practice in the beginning of the century into a worldwide recognized activity; a megaevent in terms of infrastructure and media coverage. Therefore, in a short period of time the debate about eSports become recognized as ‘sport’ as well as to be include into the Olympic Games programme emerged. In recent years, some decisions heated this debate. For example, at the occasion of the 6th Summit of the International Olympic Committee (IOC), held at Lausanne (Switzerland) in 2017, eSports were placed on the agenda about the future of the Olympic Movement. As a result of the Summit discussions, the IOC concluded that (1) eSports have been demonstrating a sharp growth with the youth of several countries and could become a platform for engagement between this young population and the Olympic Movement; (2) eSports can be considered a physical activity and the players training intensity could be compared to ‘traditional sports’ athletes; (3) to be recognized as sport by the IOC, the contents of eSports must not violate the Olympic Values; and (4) in order to the IOC grant recognition, an international eSports federation must be created to ensure the compliance of the rules and regulations of the Olympic Movement (IOC, 2017). Moreover, another important milestone was the announcement of the Intel World Open, an eSports event organized by Intel in partnership with the IOC that is going to be held in July 2020 and it will be a pre-event for the Tokyo Olympic Games. In this sense, this paper has an exploratory approach and the Content Analysis was the interpretational method used. The main results indicated three categories of understanding about the eSports and the Olympics relation: (1) the eSports does not have the necessary features to be considered ‘sport’, then could not be included in Olympic programme; (2) the eSports activity is comparable to ‘traditional sports’, therefore could be part of the Olympic programme; and (3) the eSports could have its own ‘Olympic event’, a parallel competition such as Paralympics. In this sense, the relationship between the eSports and the Olympic Movement is undeniable and despite the different arguments analysed in paper, there are other perspectives remaining to be considered. Thus, a second phase of this study is currently under development and it includes the eSports and Olympics protagonists point of view about the research topic

Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation

Lactococcus lactis is a gram positive facultative anaerobe widely used in the dairy industry and human health. L. lactis subsp. lactis NCDO 2118 is a strain that exhibits anti-inflammatory and immunomodulatory properties. In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-aerated (30°C and 37°C) conditions, respectively. Our label-free proteomic analysis quantified a total of 1,239 proteins amongst which 161 proteins were statistically differentially expressed. Main differences were observed in cellular metabolism, stress response, transcription and proteins associated to cell wall. In addition, we identified six strain-specific proteins of NCDO 2118. Altogether, the results obtained in our study will help to improve the understanding about the factors related to both physiology and adaptive processes of L. lactis NCDO 2118 under changing environmental conditions.Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Castro Oliveira, Leticia. Universidade Federal Do Triangulo Mineiro; BrasilFil: Castro Soares, Siomar. Universidade Federal Do Triangulo Mineiro; BrasilFil: Severiano Sousa, Cassiana. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Campos Tavares, Guilherme. No especifíca;Fil: Perdigão Resende, Cristiana. No especifíca;Fil: Pereira, Felipe Luis. No especifíca;Fil: Ghosh, Preetam. Virginia Commonwealth University; Estados UnidosFil: Figueiredo, Henrique. No especifíca;Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasi