Análisis de la musicalización en la publicidad televisiva de Entel Perú en el año 2016

La siguiente investigación pretende analizar la música en la publicidad televisiva de Entel Perú en el año 2016. Para ello, se clasificó y explicó los distintos tipos de música que existen en la publicidad y se identificó los elementos musicales que mayormente son utilizados. Asimismo, la investigación ha determinado la importancia que los entrevistados especialistas le otorgan a la música como estrategia publicitaria. Se analizaron diez spots lanzados en el 2016, los cuales se encuentran en el canal de YouTube de Entel Perú (escenario de la investigación).Los objetivos mencionados pudieron ser concretados gracias a la metodología cualitativa hermenéutica. Además, los siguientes instrumentos: Ficha de Análisis y Entrevista, permitieron el correcto desarrollo de los mismos y del análisis de la investigación en su totalidad. Finalmente, la buena ejecución de dichos instrumentos contribuyó en la elaboración de las conclusiones y recomendaciones

Utility of CYP2D6 copy number variants as prognostic biomarker in localized anal squamous cell carcinoma

CYP2D6; Anal squamous cell carcinoma; Cell cycleCYP2D6; Carcinoma de células escamosas anales; Ciclo celularCYP2D6; Carcinoma anal de cèl·lules escamoses; Cicle cel·lularBackground: Anal squamous cell carcinoma (ASCC) is an infrequent tumor whose treatment has not changed since the 1970s. The aim of this study is the identification of biomarkers allowing personalized treatments and improvement of therapeutic outcomes. Methods: Forty-six paraffin tumor samples from ASCC patients were analyzed by whole-exome sequencing. Copy number variants (CNVs) were identified and their relation to disease-free survival (DFS) was studied and validated in an independent retrospective cohort of 101 ASCC patients from the Multidisciplinary Spanish Digestive Cancer Group (GEMCAD). GEMCAD cohort proteomics allowed assessing the biological features of these tumors. Results: On the discovery cohort, the median age was 61 years old, 50% were males, stages I/II/III: 3 (7%)/16 (35%)/27 (58%), respectively, median DFS was 33 months, and overall survival was 45 months. Twenty-nine genes whose duplication was related to DFS were identified. The most representative was duplications of the CYP2D locus, including CYP2D6, CYP2D7P, and CYP2D8P genes. Patients with CYP2D6 CNV had worse DFS at 5 years than those with two CYP2D6 copies (21% vs. 84%; p < .0002, hazard ratio [HR], 5.8; 95% confidence interval [CI], 2.7-24.9). In the GEMCAD validation cohort, patients with CYP2D6 CNV also had worse DFS at 5 years (56% vs. 87%; p = .02, HR = 3.6; 95% CI, 1.1-5.7). Mitochondria and mitochondrial cell-cycle proteins were overexpressed in patients with CYP2D6 CNV. Conclusions: Tumor CYP2D6 CNV identified patients with a significantly worse DFS at 5 years among localized ASCC patients treated with 5-fluorouracil, mitomycin C, and radiotherapy. Proteomics pointed out mitochondria and mitochondrial cell-cycle genes as possible therapeutic targets for these high-risk patients. Plain language summary: Anal squamous cell carcinoma is an infrequent tumor whose treatment has not been changed since the 1970s. However, disease-free survival in late staged tumors is between 40% and 70%. The presence of an alteration in the number of copies of CYP2D6 gene is a biomarker of worse disease-free survival. The analysis of the proteins in these high-risk patients pointed out mitochondria and mitochondrial cell-cycle genes as possible therapeutic targets. Therefore, the determination of the number of copies of CYP2D6 allows the identification of anal squamous carcinoma patients with a high-risk of relapse that could be redirected to a clinical trial. Additionally, this study may be useful to suggest new treatment strategies to increase current therapy efficacy

Manual de procedimientos administrativos y financieros de la empresa de servicios de asesoría integral Zuorpace Cía. Ltda de la ciudad de Quito, provincia de Pichincha

Diseñar un manual de procedimientos administrativos y financieros de la empresa de Servicios de Asesoría Integral ZUORPACE Cía. Ltda., mediante investigación científica y de campo para mejorar los procedimientos dentro de la empresa.El presente trabajo de grado tiene como objetivo principal, desarrollar un manual de procedimientos administrativos y financieros, para la empresa de Asesoría Integral ZUORPACE Cía. Ltda., el mismo que será de suma importancia para mejorar la calidad en el desarrollo de las actividades cotidianas propias del giro del negocio. Mediante un diagnóstico situacional aplicado a la empresa, se logró recopilar información valiosa para conocer a profundidad la necesidad de dicho manual, mismo que está respaldado por las bases teóricas obtenidas de fuentes bibliográficas, linkográficas, entre otros, que garantizan el desarrollo de un trabajo eficiente y profesional. Con toda la información recopilada se procedió a realizar la propuesta misma que contiene aspectos administrativos de la empresa como es la filosofía, normatividad y perfiles profesionales; manuales de procedimientos con las actividades consideradas las más importantes y por último un manual financiero con dinámicas de cuentas, modelos de análisis e indicadores financieros. Para conocer cuáles son los impactos que generará la implementación de dicho trabajo se ha realizado un análisis detallado en cuanto al ámbito social, educativo, económico y ambiental considerado como los más relevantes

Identificación de factores familiares que determinan la desición de compra de productos lácteos en el cantón Salcedo.

The present reseach has for aim identify the familiar factors which determine the decision to purchase lacteal products in the salcedo town, into the problem that was identified was the ignorance of familiar factors in the population, it is inquired the theoretical bases that support the information, about the familiar factors that affect in the decision to purchase lacteals products customers, obtaining relevant information about consumer behavior, the purchase decision and the process that consumers perform at the moment to decide what they will acquire. The analysis was based on quantitative research, for the statistical study was used the SPSS program, to do the lifting of the information was modidied a survey an invetigation of the authors Bravo, Fraj & Martínez, to the same extracted the questions that helped identify the familiar factors of purchase decision. The results obtained from the research tried to find the fundamental reasons that the consumer Salcedence pursues to buy the family consumption products, It has been made a relationship between the questions through dynamic tables for general a reliability in the information that was obtained with the survey application, through analysis, it was possible to determine which are the most relevant factors that the population considers when decide their purchase.La presente investigación tiene por objetivo identificar cuáles son los factores familiares que determinan la decisión de compra de los clientes de productos lácteos en el cantón Salcedo, dentro del problema que se identifico fue el desconocimiento de factores familiares en la población, se indago las bases teóricas que sustentan la información, acerca de los factores familiares que inciden en la decisión de compra de los clientes de productos lácteos, obteniendo información relevante sobre el comportamiento del consumidor, la decisión de compra y el proceso que los consumidores ejecutan al momento de decidir lo que van a adquirir. El análisis se sustentó en una investigación cuantitativa, para el estudio estadístico se utilizó el programa SPSS, para realizar el levantamiento de la información se modificó una encuesta de una investigación de los autores Bravo, Fraj & Martínez, a la misma se extrajo las preguntas que ayudaron a identificar los factores familiares de decisión de compra. Los resultados obtenidos de la presente investigación pretendían encontrar las razones fundamentales que el consumidor Salcedence persigue para comprar los productos de consumo familiar, se realizó una relación entre las preguntas a través de tablas dinámicas para general una fiabilidad en la información que se obtuvo con la aplicación de la encuesta, mediante análisis se logró determinar cuáles son los factores más relevantes que la población considera al decidir su compra.Universidad Técnica de Cotopax

Aplicación de la sección 28 beneficios a los empleados de la normas internacionales de información financiera para la pequeña y mediana empresa (NIIF para Pymes), en la empresa DHL Nicaragua S.A en el periodo finalizado

La presente investigación de seminario de graduación se elaboró con el propósito de evaluar los criterios contables apegados a la normativa NIIF para Pymes sección 28 “beneficios a los empleados” y que estos procesos cumplan con la ley de regulación vigente de Nicaragua, anexo a lo planteado en el trabajo del marco legal es imprescindible tomar en consideración los beneficios que los trabajadores tienen por naturaleza de ley y que estas están soportadas en: Ley Nº 822 “Concertación tributaria”, Ley Nº 185 “Código del trabajo” y la Ley Nº 539 “Seguridad social”, para informarnos acerca de las contraprestaciones legales y procesarlos contablemente. El método utilizado fue el método investigativo con enfoque de estudio de la sección 28 “beneficios a los empleados” y el giro de la entidad, se aplicó un caso práctico a DHL NICARAGUA S, A, con el fin de obtener mejores resultados aplicando las leyes de seguridad social muy importantes dentro de las obligaciones e intereses de cualquier compañía de pérdida de las cuentas por pagar las cuales juegan un rol muy importante de cualquier compañía y trabajadores a cambio de sus servicios. Se concluyó que la importancia que tiene la sección 28 “beneficios a los empleados”, ya que se determinó y hacemos notar que al aplicar correctamente tanto leyes como normas, nos convierte a nosotros como responsables de realizar los procesos correctos en beneficio del empleador y colaborador, para el empleador es importante que se cumplan en su operatividad administrativa las regulaciones, leyes, controles internos, puntos de auditoria que puedan ser evaluadas por parte de los entes reguladores gubernamentales esos son puntos muy importantes que toda empresa debe tomar en consideración para su operatividad económica

Transcriptome analysis of Neofusicoccum luteum during avocado branch and fruit infection

The most important aerial diseases affecting avocado orchards on the Andalusian coast (Spain) are caused by species of fungi belonging to the Botryosphaeriaceae family, one of the most common being Neofusicoccum luteum. The symptoms produced by this fungus are branch dieback and fruit rot. Currently, it is essential to develop strategies to control this disease. In this line, knowledge of the infection mechanisms of the pathogen is considered an essential objective. Therefore, a transcriptomic study was carried out by RNAseq of N. luteum growing on avocado branch and fruit in comparison with its in vitro growth in PDA medium. Transcriptome analysis revealed a total of 903 and 1271 genes significantly downregulated (- 2 > fold change > 2) during growth on branch and fruit compared to growth on PDA, respectively. Among the genes overexpressed in the N. luteum/branch/fruit interaction, genes related to mycotoxin production, wall degradation, detoxification of harmful compounds, protein degradation and candidate effector proteins were identified, three of which showed 100% probability (Effector P3) and apoplastic localization. The analysis of N. luteum transcriptome during the infection process will be a very useful tool to better understand the biology and virulence of this emerging pathogen and will help in the development of strategies for its controlUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Evaluation of the Performance of the IDvet IFN-Gamma Test for Diagnosis of Bovine Tuberculosis in Spain

In Spain, the national bovine tuberculosis (bTB) eradication program is based on yearly skin testing of every ≥6 weeks old animal using the single or comparative tuberculin test and parallel use of the interferon-gamma (IFN-γ) assay as an ancillary diagnostic test in infected herds. There are several versions of the latter. Recently, a new commercial IDvet IFN-γ assay has been authorized for use in the program, but there is limited scientific evidence about its performance in different epidemiological settings. Therefore, two studies to evaluate the performance of the IDvet assay were conducted. In study 1, a concordance analysis between the new IDvet and the Bovigam IFN-γ assay in use in Spain for over 10 years was conducted. In study 2, results from the IDvet assay when applied in tandem with a single intradermal tuberculin (SIT) test were used to evaluate the concordance between both tests and to estimate their sensitivity (Se) and specificity (Sp) using a Bayesian latent-class model. Field data from cattle herds located in Madrid and Castilla y Leon (Spain) were collected. For study 1, herd selection was based on a high expected prevalence of reactors to the IFN-γ assay, while herds were selected at random to estimate Se and Sp of the new IDvet assay in study 2. Agreement between the results obtained with both kits for IFN-γ assay was poor (Kappa = 0.20), and a receiver operating characteristic (ROC) analysis indicated a low Se of the new IDvet relative to the Bovigam in a heavily bTB infected population. The Bayesian latent-class analysis estimated the Se of the IDvet assay to be 36.7% [95% probability posterior interval (PPI) 14.7–78.8%] with estimated Sp close to 100% when the cut-off recommended by the manufacturer (35) was applied. At the alternative cut-off values of 16 and 4, the estimated Se of the IDvet assay increased to 49.0% (PPI: 24.8–94.1%) and 56.0% (PPI: 30.8–96.3%), respectively, while maintaining a high specificity. The results suggest that the new IDvet assay may have lower sensitivity than the Bovigam for diagnosis of bTB in cattle herds in Spain, and that adjusting its cut-off might be considered

Anti-CXCR4 Antibody Combined With Activated and Expanded Natural Killer Cells for Sarcoma Immunotherapy

Sarcoma is one of the most severe forms of pediatric cancer and current therapies -chemotherapy and surgery- fail to eradicate the disease in half of patients. Preclinical studies combining new therapeutic approaches can be useful to design better therapies. On one hand, it is known that CXCR4 expression is implicated in rhabdomyosarcoma progression, so we analyzed relapses and chemotherapy-resistant rhabdomyosarcoma tumors from pediatric patients and found that they had particularly high levels of CXCR4 expression. Moreover, in assays in vitro, anti-CXCR4 blocking antibody (MDX1338) efficiently reduced migration and invasion of alveolar rhabdomyosarcoma RH30 cells. On the other hand, activated and expanded natural killer (NKAE) cell therapy showed high cytotoxicity against sarcoma cells in vitro and completely inhibited RH30 tumor implantation in vivo. Only the combination of MDX1338 and NKAE treatments completely suppressed metastasis in mice. In this study, we propose a novel therapeutic approach based on anti-CXCR4 blocking antibody in combination with NKAE cell therapy to prevent rhabdomyosarcoma tumor implantation and lung metastasis. These results provide the first evidence for the efficacy of this combined immunotherapy for preventing sarcoma disease dissemination.This work was supported in part by the National Health Service of Spain, Instituto de Salud Carlos III (ISCIII), FONDOS FEDER grant (FIS) PI15/00973; Asociacion Espanola Contra el Cancer to AP-M; CRIS Foundation to Beat Cancer grant to JV, LF, and AE; and Patients' Support Associations Fundacion Mari Paz Jimenez Casado and La Sonrisa de Alex to MV and the research project

Effects of physical exercise on physical function in older adults in residential care: a systematic review and network meta-analysis of randomised controlled trials

Background: Physical exercise is effective at attenuating ageing-related physical decline in general, but evidence of its benefits for older adults in residential care, who often have functional dependency, multimorbidity, and polypharmacy, is inconclusive. We aimed to establish the effects of exercise interventions on the physical function of this population. Methods: For this systematic review and network meta-analysis, we searched PubMed, Web of Science, Cochrane Library, Rehabilitation & Sports Medicine Source, and SPORTDiscus to identify randomised controlled trials assessing the effects of exercise interventions (vs usual care) on physical function (ie, functional independence, physical performance, and other related measures, such as muscle strength, balance, or flexibility) in adults aged 60 years or older living in residential care. Relevant studies published in English or Spanish up to Jan 12, 2023, were included in the systematic review. The quality of studies was assessed using the Tool for the Assessment of Study Quality and Reporting in Exercise (TESTEX) score. A network meta-analysis was performed for physical function-related outcomes reported in at least ten studies, with subanalyses for specific intervention (ie, exercise type, training volume, and study duration) and participant (eg, having cognitive impairment or dementia, pre-frail or frail status, and being functionally dependent) characteristics. The study protocol was registered on PROSPERO (CRD42021247809). Findings: 147 studies (11 609 participants, with mean ages ranging from 67 years [SD 9] to 92 years [2]) were included in the systematic review, and were rated as having overall good quality (median TESTEX score 9 [range 3–14]). In the meta-analysis (including 105 studies, n=7759 participants), exercise interventions were associated with significantly improved overall physical function, with a standardised mean difference [SMD] of 0·13 (95% credible interval [CrI] 0·04–0·21), which was confirmed in all analysed subpopulations. The strongest association was observed with 110–225 min per week of exercise, and the greatest improvements were observed with 170 min per week (SMD 0·36 [95% CrI 0·20–0·52]). No significant differences were found between exercise types. Subanalyses showed significant improvements for almost all analysed physical function-related outcomes (Barthel index, five-times sit-to-stand test, 30-s sit-to-stand test, knee extension, hand grip strength, bicep curl strength, Short Physical Performance Battery, 6-min walking test, walking speed, Berg balance scale, and sit-and-reach test). Large heterogeneity was found between and within studies in terms of population and intervention characteristics. Interpretation: Exercise interventions are associated with improved physical function in older adults in residential care, and should, therefore, be routinely promoted in long-term care facilities. Funding: None. Translation: For the Spanish translation of the abstract see Supplementary Materials section.10 página

Client applications and Server Side docker for management of RNASeq and/or VariantSeq workflows and pipelines of the GPRO Suite

The GPRO suite is an in-progress bioinformatic project for -omic data analyses. As part of the continued growth of this project, we introduce a client side & server side solution for comparative transcriptomics and analysis of variants. The client side consists of two Java applications called "RNASeq" and "VariantSeq" to manage workflows for RNA-seq and Variant-seq analysis, respectively, based on the most common command line interface tools for each topic. Both applications are coupled with a Linux server infrastructure (named GPRO Server Side) that hosts all dependencies of each application (scripts, databases, and command line interface tools). Implementation of the server side requires a Linux operating system, PHP, SQL, Python, bash scripting, and third-party software. The GPRO Server Side can be deployed via a Docker container that can be installed in the user's PC using any operating system or on remote servers as a cloud solution. The two applications are available as desktop and cloud applications and provide two execution modes: a Step-by-Step mode enables each step of a workflow to be executed independently and a Pipeline mode allows all steps to be run sequentially. The two applications also feature an experimental support system called GENIE that consists of a virtual chatbot/assistant and a pipeline jobs panel coupled with an expert system. The chatbot can troubleshoot issues with the usage of each tool, the pipeline job panel provides information about the status of each task executed in the GPRO Server Side, and the expert provides the user with a potential recommendation to identify or fix failed analyses. The two applications and the GPRO Server Side combine the user-friendliness and security of client software with the efficiency of front-end & back-end solutions to manage command line interface software for RNA-seq and variant-seq analysis via interface environments