Keeping a foot in both camps: Sustainability, city branding and boundary spanners

This study critically examines sustainable development (SD) within the contemporary practices of city branding, a prominent business philosophy that underpins market-led development strategies of urban areas. In pursuing uniqueness, different cities often seem to hint at the very same themes of differentiation, and this reflects the tendency to embrace pre-given sets of place-development discourses. This work casts a critical perspective on SD as one of the global passe-partout themes that has become particularly prominent in contemporary city-brand management practices. In particular, the theory-practice gap in city branding for SD is emphasized and interpreted through the lens of glocalization theories. This viewpoint identifies responsibilized boundary spanners as agents located between the global and local levels that act as mediators in multi-stakeholder networks, ultimately fostering capacities to implement collective actions in city-branding practices

Fabrication and First Full Characterisation of Timing Properties of 3D Diamond Detectors

Tracking detectors at future high luminosity hadron colliders are expected to be able to stand unprecedented levels of radiation as well as to efficiently reconstruct a huge number of tracks and primary vertices. To face the challenges posed by the radiation damage, new extremely radiation hard materials and sensor designs will be needed, while the track and vertex reconstruction problem can be significantly mitigated by the introduction of detectors with excellent timing capabilities. Indeed, the time coordinate provides extremely powerful information to disentangle overlapping tracks and hits in the harsh hadronic collision environment. Diamond 3D pixel sensors optimised for timing applications provide an appealing solution to the above problems as the 3D geometry enhances the already outstanding radiation hardness and allows to exploit the excellent timing properties of diamond. We report here the first full timing characterisation of 3D diamond sensors fabricated by electrode laser graphitisation in Florence. Results from a 270MeV pion beam test of a first prototype and from tests with a β source on a recently fabricated 55×55μm2 pitch sensor are discussed. First results on sensor simulation are also presented

“N-alkyl diketopyrrolopyrrole-based fluorophores for luminescent solar concentrators: effect of the alkyl chain on dye efficiency”

We report on the preparation of luminescent solar concentrators (LSCs) made of poly(methyl methacrylate) (PMMA) thin films doped with six new diketopyrrolopyrrole (DPP) fluorophores obtained in good yields by using simple N-alkylation and direct C-H arylation synthetic strategies. Spectroscopic investigations in solution and in PMMA thin films combined with photocurrent measurements revealed that the branched alkyl chains were efficient in preventing DPP segregation from the PMMA matrix thanks to their higher steric hindrance. The aromatic substituent was found to expand DPP conjugation but favoured DPP adverse aggregation, thus affecting fluorescence emission and photocurrents of PMMA films. The worthwhile combination of the appropriate alkyl chain and aromatic moieties assured to achieve optical efficiency of 6.8% that was comparable to that of PMMA LSCs of the state-of-the art (7.2%)

Anomalies in Dopamine Transporter Expression and Primary Cilium Distribution in the Dorsal Striatum of a Mouse Model of Niemann-Pick C1 Disease

The Niemann-Pick type C1 (NPC1) is a rare genetic disease characterized by the accumulation of endocytosed cholesterol and other lipids in the endosome/lysosome compartments. In the brain, the accumulation/mislocalization of unesterified cholesterol, gangliosides and sphingolipids is responsible for the appearance of neuropathological hallmarks, and progressive neurological decline in patients. The imbalance of unesterified cholesterol and other lipids, including GM2 and GM3 gangliosides, alters a number of signaling mechanisms impacting on the overall homeostasis of neurons. In particular, lipid depletion experiments have shown that lipid rafts regulate the cell surface expression of dopamine transporter (DAT) and modulate its activity. Dysregulated dopamine transporter’s function results in imbalanced dopamine levels at synapses and severely affects dopamine-induced locomotor responses and dopamine receptor-mediated synaptic signaling. Recent studies begin to correlate dopaminergic stimulation with the length and function of the primary cilium, a non-motile organelle that coordinates numerous signaling pathways. In particular, the absence of dopaminergic D2 receptor stimulation induces the elongation of dorso-striatal neuron’s primary cilia. This study has used a mouse model of the NPC1 disease to correlate cholesterol dyshomeostasis with dorso-striatal anomalies in terms of DAT expression and primary cilium (PC) length and morphology. We found that juvenile Npc1nmf164 mice display a reduction of dorso-striatal DAT expression, with associated alterations of PC number, length-frequency distribution, and tortuosity

The emerging role of cancer nanotechnology in the panorama of sarcoma

In the field of nanomedicine a multitude of nanovectors have been developed for cancer application. In this regard, a less exploited target is represented by connective tissue. Sarcoma lesions encompass a wide range of rare entities of mesenchymal origin affecting connective tissues. The extraordinary diversity and rarity of these mesenchymal tumors is reflected in their classification, grading and management which are still challenging. Although they include more than 70 histologic subtypes, the first line-treatment for advanced and metastatic sarcoma has remained unchanged in the last fifty years, excluding specific histotypes in which targeted therapy has emerged. The role of chemotherapy has not been completely elucidated and the outcomes are still very limited. At the beginning of the century, nano-sized particles clinically approved for other solid lesions were tested in these neoplasms but the results were anecdotal and the clinical benefit was not substantial. Recently, a new nanosystem formulation NBTXR3 for the treatment of sarcoma has landed in a phase 2-3 trial. The preliminary results are encouraging and could open new avenues for research in nanotechnology. This review provides an update on the recent advancements in the field of nanomedicine for sarcoma. In this regard, preclinical evidence especially focusing on the development of smart materials and drug delivery systems will be summarized. Moreover, the sarcoma patient management exploiting nanotechnology products will be summed up. Finally, an overlook on future perspectives will be provided

Fabrication and Characterisation of 3D Diamond Pixel Detectors With Timing Capabilities

Diamond sensors provide a promising radiation hard solution to the challenges posed by the future experiments at hadron machines. A 3D geometry with thin columnar resistive electrodes orthogonal to the diamond surface, obtained by laser nanofabrication, is expected to provide significantly better time resolution with respect to the extensively studied planar diamond sensors. We report on the development, production, and characterisation of innovative 3D diamond sensors achieving 30% improvement in both space and time resolution with respect to sensors from the previous generation. This is the first complete characterisation of the time resolution of 3D diamond sensors and combines results from tests with laser, beta rays and high energy particle beams. Plans and strategies for further improvement in the fabrication technology and readout systems are also discussed

Qualitative assessment of contrast-enhanced ultrasound in differentiating clear cell renal cell carcinoma and oncocytoma

Background: We aimed to assess whether clear cell renal cell carcinoma (ccRCC) can be differentiated from renal oncocytoma (RO) on a contrast-enhanced ultrasound (CEUS). Methods: Between January 2021 and October 2022, we retrospectively queried and analyzed our prospectively maintained dataset. Renal mass features were scrutinized with conventional ultrasound imaging (CUS) and CEUS. All lesions were confirmed by histopathologic diagnoses after nephron-sparing surgery (NSS). A multivariable analysis was performed to identify the potential predictors of ccRCC. The area under the curve (AUC) was depicted in order to assess the diagnostic accuracy of the multivariable model. Results: A total of 126 renal masses, including 103 (81.7%) ccRCC and 23 (18.3%) RO, matched our inclusion criteria. Among these two groups, we found significant differences in terms of enhancement (homogeneous vs. heterogeneous) (p < 0.001), wash-in (fast vs. synchronous/slow) (p = 0.004), wash-out (fast vs. synchronous/slow) (p = 0.001), and rim-like enhancement (p < 0.001). On the multivariate logistic regression, heterogeneous enhancement (OR: 19.37; p = <0.001) and rim-like enhancement (OR: 3.73; p = 0.049) were independent predictors of ccRCC. Finally, these two variables had an AUC of 82.5% and 75.3%, respectively. Conclusions: Diagnostic imaging for presurgical planning is crucial in the choice of either conservative or radical management. CEUS, with its unique features, revealed its usefulness in differentiating ccRCC from RO

Timing Performances and Radiation Hardness of 3D Diamond Detectors

n/

Renal progenitor cells revert LPS-induced endothelial-to-mesenchymal transition by secreting CXCL6, SAA4, and BPIFA2 antiseptic peptides

Endothelial dysfunction is a hallmark of LPS-induced acute kidney injury (AKI). Endothelial cells (ECs) acquired a fibroblast-like phenotype and contributed to myofibroblast generation through the endothelial-to-mesenchymal transition (EndMT) process. Of note, human adult renal stem/progenitor cells (ARPCs) enhance the tubular regenerative mechanism during AKI but little is known about their effects on ECs. Following LPS exposure, ECs proliferated, decreased EC markers CD31 and vascular endothelial cadherin, and up-regulated myofibroblast markers, collagen I, and vimentin. The coculture with ARPCs normalized the EC proliferation rate and abrogated the LPS-induced EndMT. The gene expression analysis showed that most of the genes modulated in LPS-stimulated ARPCs belong to cell activation and defense response pathways. We showed that the ARPC-specific antifibrotic effect is exerted by the secretion of CXCL6, SAA4, and BPIFA2 produced after the anaphylatoxin stimulation. Next, we investigated the molecular signaling that underlies the ARPC protective mechanism and found that renal progenitors diverge from differentiated tubular cells and ECs in myeloid differentiation primary response 88-independent pathway activation. Finally, in a swine model of LPS-induced AKI, we observed that activated ARPCs secreted CXCL6, SAA4, and BPIFA2 as a defense response. These data open new perspectives on the treatment of both sepsis- and endotoxemia-induced AKI, suggesting an underestimated role of ARPCs in preventing endothelial dysfunction and novel strategies to protect the endothelial compartment and promote kidney repair.-Sallustio, F., Stasi, A., Curci, C., Divella, C., Picerno, A., Franzin, R., De Palma, G., Rutigliano, M., Lucarelli, G., Battaglia, M., Staffieri, F., Crovace, A., Pertosa, G. B., Castellano, G., Gallone, A., Gesualdo, L. Renal progenitor cells revert LPS-induced endothelial-to-mesenchymal transition by secreting CXCL6, SAA4, and BPIFA2 antiseptic peptides