Analyzing the Catalytic Role of Asp97 in the Methionine Aminopeptidase from \u3cem\u3eEscherichia coli\u3c/em\u3e

An active site aspartate residue, Asp97, in the methionine aminopeptidase (MetAPs) from Escherichia coli (EcMetAP-I) was mutated to alanine, glutamate, and asparagine. Asp97 is the lone carboxylate residue bound to the crystallographically determined second metal-binding site in EcMetAP-I. These mutant EcMetAP-I enzymes have been kinetically and spectroscopically characterized. Inductively coupled plasma–atomic emission spectroscopy analysis revealed that 1.0 ± 0.1 equivalents of cobalt were associated with each of the Asp97-mutated EcMetAP-Is. The effect on activity after altering Asp97 to alanine, glutamate or asparagine is, in general, due to a ∼ 9000-fold decrease in kca towards Met-Gly-Met-Met as compared to the wild-type enzyme. The Co(II) d–d spectra for wild-type, D97E and D97A EcMetAP-I exhibited very little difference in form, in each case, between the monocobalt(II) and dicobalt(II) EcMetAP-I, and only a doubling of intensity was observed upon addition of a second Co(II) ion. In contrast, the electronic absorption spectra of [Co_(D97N EcMetAP-I)] and [CoCo(D97N EcMetAP-I)] were distinct, as were the EPR spectra. On the basis of the observed molar absorptivities, the Co(II) ions binding to the D97E, D97A and D97N EcMetAP-I active sites are pentacoordinate. Combination of these data suggests that mutating the only nonbridging ligand in the second divalent metal-binding site in MetAPs to an alanine, which effectively removes the ability of the enzyme to form a dinuclear site, provides a MetAP enzyme that retains catalytic activity, albeit at extremely low levels. Although mononuclear MetAPs are active, the physiologically relevant form of the enzyme is probably dinuclear, given that the majority of the data reported to date are consistent with weak cooperative binding

Operator Product Expansion in the Production and Decay of the X(3872)

The X(3872) seems to be a weakly-bound hadronic molecule whose constituents are two charm mesons. Its binding energy is much smaller than all the other energy scales in QCD. This separation of scales can be exploited through factorization formulas for production and decay rates of the X. In a low-energy effective field theory for the constituents of the X, the factorization formulas can be derived using the operator product expansion. The derivations are carried out explicitly for the simplest effective theory in which the constituents interact through a contact interaction that produces a large scattering length. The long-distance factors in the operator product expansions for various observables are calculated nonperturbatively in the interaction strength of the contact interaction. After renormalization of the coupling constant, all remaining ultraviolet divergences can be absorbed into the short-distance factors in the operator product expansions.Comment: 30 pages, 5 figure

Single deep ultraviolet light emission from boron nitride nanotube film

Light in deep ultraviolet DUV region has a wide range of applications and the demand for finding DUV light emitting materials at nanoscale is increasingly urgent as they are vital for building miniaturized optic and optoelectronic devices. We discover that boron nitride nanotubes BNNTs with a well-crystallized cylindrical multiwall structure and diameters smaller than 10 nm can have single DUV emission at 225 nm 5.51 eV. The measured BNNTs are grown on substrate in the form of a thin film. This study suggests that BNNTs may work as nanosized DUV light sources for various applications. © 20

On the low frequency acoustic properties of novel multifunctional honeycomb sandwich panels with micro-perforated faceplates

This paper explores further possibilities of structurally-efficient honeycomb sandwich panels by replacing one of the faceplates with the perforated faceplate from the viewpoint of sound absorption coefficient (SAC) as well as sound transmission loss (STL). An analytical model is presented to calculate both the STL and SAC, with the displacements of the two faceplates assumed identical at frequencies below the faceplate resonance frequency. Influences of core configuration are investigated by comparing different honeycomb core designs. Finite element (FE) models are subsequently developed to validate the proposed analytical model, with agreement achieved. Subsequently, parametric surveys, including the influences of perforation ratio, pore size and core configuration on STL and SAC, are conducted based on the analytical model. Unlike classical honeycomb sandwich panels which are poor sound absorbers, honeycomb sandwiches with perforated faceplates lead to high SAC at low frequencies, which in turn brings about increment in the low frequency STL. Moreover, sandwich panels with triangular cores are found to have the lowest peak frequency in the STL and SAC curves compared with the other kinds of sandwich panels having the same effective mass and perforations

Bandwidth and Electron Correlation-Tuned Superconductivity in Rb0.8_{0.8}Fe2_{2}(Se1−z_{1-z}Sz_z)2_2

We present a systematic angle-resolved photoemission spectroscopy study of the substitution-dependence of the electronic structure of Rb$_{0.8}$Fe$_{2}$(Se$_{1-z}$S$_z$)$_2$ (z = 0, 0.5, 1), where superconductivity is continuously suppressed into a metallic phase. Going from the non-superconducting Rb$_{0.8}$Fe$_{2}$(Se$_{1-z}$S$_z$)$_2$ to superconducting Rb$_{0.8}$Fe$_{2}$Se$_2$, we observe little change of the Fermi surface topology, but a reduction of the overall bandwidth by a factor of 2 as well as an increase of the orbital-dependent renormalization in the $d_{xy}$ orbital. Hence for these heavily electron-doped iron chalcogenides, we have identified electron correlation as explicitly manifested in the quasiparticle bandwidth to be the important tuning parameter for superconductivity, and that moderate correlation is essential to achieving high $T_C$

Small perforations in corrugated sandwich panel significantly enhance low frequency sound absorption and transmission loss

Numerical and experimental investigations are performed to evaluate the low frequency sound absorption coefficient (SAC) and sound transmission loss (STL) of corrugated sandwich panels with different perforation configurations, including perforations in one of the face plates, in the corrugated core, and in both the face plate and the corrugated core. Finite element (FE) models are constructed with considerations of acoustic-structure interactions and viscous and thermal energy dissipations inside the perforations. The validity of FE calculations is checked against experimental measurements with the tested samples provided by additive manufacturing. Compared with the classical corrugated sandwich without perforation, the corrugated sandwich with perforated pores in one of its face plate not only exhibits a higher SAC at low frequencies but also a better STL as a consequence of the enlarged SAC. The influences of perforation diameter and perforation ratio on the vibroacoustic performance of the sandwich are also explored. For a corrugated sandwich with uniform perforations, the acoustical resonance frequencies and bandwidth in its SAC and STL curves decrease with increasing pore diameter and decreasing perforation ratio. Non-uniform perforation diameters and perforation ratios result in larger bandwidth and lower acoustical resonance frequencies relative to the case of uniform perforations. The proposed perforated sandwich panels with corrugated cores are attractive ultralightweight structures for multifunctional applications such as simultaneous load-bearing, energy absorption, sound proofing and sound absorption

Absorption Cross Sections of NH_3, NH_2D, NHD_2, and ND_3 in the Spectral Range 140-220 nm and Implications for Planetary Isotopic Fractionation

Cross sections for photoabsorption of NH_3, NH_2D, NHD_2, and ND_3 in the spectral region 140-220 nm were determined at ~298 K using synchrotron radiation. Absorption spectra of NH_2D and NHD_2 were deduced from spectra of mixtures of NH_3 and ND_3, of which the equilibrium concentrations for all four isotopologues obey statistical distributions. Cross sections of NH_2D, NHD_2, and ND_3 are new. Oscillator strengths, an integration of absorption cross sections over the spectral lines, for both A ← X and B ← X systems of NH_3 agree satisfactorily with previous reports; values for NH_2D, NHD_2, and ND_3 agree with quantum chemical predictions. The photolysis of NH_3 provides a major source of reactive hydrogen in the lower stratosphere and upper troposphere of giant planets such as Jupiter. Incorporating the measured photoabsorption cross sections of NH_3 and NH_2D into the Caltech/JPL photochemical diffusive model for the atmosphere of Jupiter, we find that the photolysis efficiency of NH_2D is lower than that of NH_3 by as much as 30%. The D/H ratio in NH_2D/NH_3 for tracing the microphysics in the troposphere of Jupiter is also discussed