Determination of Soybean Oil, Protein and Amino Acid Residues in Soybean Seeds by High Resolution Nuclear Magnetic Resonance (NMRS) and Near Infrared (NIRS)

A detailed account is presented of our high resolution nuclear magnetic resonance (HR-NMR) and near infrared (NIR) calibration models, methodologies and validation procedures, together with a large number of composition analyses for soybean seeds. NIR calibrations were developed based on both HR-NMR and analytical chemistry reference data for oil and twelve amino acid residues in mature soybeans and soybean embryos. This is our first report of HR-NMR determinations of amino acid profiles of proteins from whole soybean seeds, without protein extraction from the seed. It was found that the best results for both oil and protein calibrations were obtained with a Partial Least Squares Regression (PLS-1) analysis of our extensive NIR spectral data, acquired with either a DA7000 Dual Diode Array (Si and InGaAs detectors) instrument or with several Fourier Transform NIR (FT-NIR) spectrometers equipped with an integrating sphere/InGaAs detector accessory. In order to extend the bulk soybean samples calibration models to the analysis of single soybean seeds, we have analized in detail the component NIR spectra of all major soybean constituents through spectral deconvolutions for bulk, single and powdered soybean seeds. Baseline variations and light scattering effects in the NIR spectra were corrected, respectively, by calculating the first-order derivatives of the spectra and the Multiplicative Scattering Correction (MSC). The single soybean seed NIR spectra are broadly similar to those of bulk whole soybeans, with the exception of minor peaks in single soybean NIR spectra in the region from 950 to 1,000 nm. Based on previous experience with bulk soybean NIR calibrations, the PLS-1 calibration model was selected for protein, oil and moisture calibrations that we developed for single soybean seed analysis. In order to improve the reliability and robustness of our calibrations with the PLS-1 model we employed standard samples with a wide range of soybean constituent compositions: from 34% to 55% for protein, from 11% to 22% for oil and from 2% to 16% for moisture. Such calibrations are characterized by low standard errors and high degrees of correlation for all major soybean constituents. Morever, we obtained highly resolved NIR chemical images for selected regions of mature soybean embryos that allow for the quantitation of oil and protein components. Recent developments in high-resolution FT-NIR microspectroscopy extend the NIR sensitivity range to the picogram level, with submicron spatial resolution in the component distribution throughout intact soybean seeds and embryos. Such developments are potentially important for biotechnology applications that require rapid and ultra- sensitive analyses, such as those concerned with high-content microarrays in Genomics and Proteomics research. Other important applications of FT-NIR microspectroscopy are envisaged in biomedical research aimed at cancer prevention, the early detection of tumors by NIR-fluorescence, and identification of single cancer cells, or single virus particles in vivo by super-resolution microscopy/ microspectroscopy

LANDSAT-4/5 image data quality analysis

A LANDSAT Thematic Mapper (TM) quality evaluation study was conducted to identify geometric and radiometric sensor errors in the post-launch environment. The study began with the launch of LANDSAT-4. Several error conditions were found, including band-to-band misregistration and detector-to detector radiometric calibration errors. Similar analysis was made for the LANDSAT-5 Thematic Mapper and compared with results for LANDSAT-4. Remaining band-to-band misregistration was found to be within tolerances and detector-to-detector calibration errors were not severe. More coherent noise signals were observed in TM-5 than in TM-4, although the amplitude was generally less. The scan direction differences observed in TM-4 were still evident in TM-5. The largest effect was in Band 4 where nearly a one digital count difference was observed. Resolution estimation was carried out using roads in TM-5 for the primary focal plane bands rather than field edges as in TM-4. Estimates using roads gave better resolution. Thermal IR band calibration studies were conducted and new nonlinear calibration procedures were defined for TM-5. The overall conclusion is that there are no first order errors in TM-5 and any remaining problems are second or third order

Broad application of a simple and affordable protocol for isolating plant RNA

BACKGROUND: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. FINDINGS: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. CONCLUSIONS: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide

Power scaling rules for charmonia production and HQEFT

We discuss the power scaling rules along the lines of a complete Heavy Quark Effective Field Theory (HQEFT) for the description of heavy quarkonium production through a color-octet mechanism. To this end, we firstly derive a tree-level heavy quark effective Lagrangian keeping both particle-antiparticle mixed sectors allowing for heavy quark-antiquark pair annihilation and creation, but describing only low-energy modes around the heavy quark mass. Then we show the consistency of using HQEFT fields in constructing four-fermion local operators a la NRQCD, to be identified with standard color-octet matrix elements. We analyze some numerical values extracted from charmonia production by different authors and their hierarchy in the light of HQEFT.Comment: LaTeX, 19 pages, 3 EPS figure

Experimental observation of fractional topological phases with photonic qudits

Geometrical and topological phases play a fundamental role in quantum theory. Geometric phases have been proposed as a tool for implementing unitary gates for quantum computation. A fractional topological phase has been recently discovered for bipartite systems. The dimension of the Hilbert space determines the topological phase of entangled qudits under local unitary operations. Here we investigate fractional topological phases acquired by photonic entangled qudits. Photon pairs prepared as spatial qudits are operated inside a Sagnac interferometer and the two-photon interference pattern reveals the topological phase as fringes shifts when local operations are performed. Dimensions $d = 2, 3$ and $4$ were tested, showing the expected theoretical values.Comment: 6 pages, 4 figure

Static properties of the dissipative random quantum Ising ferromagnetic chain

We study the zero temperature static properties of dissipative ensembles of quantum Ising spins arranged on periodic one dimensional finite clusters and on an infinite chain. The spins interact ferro-magnetically with nearest-neighbour pure and random couplings. They are subject to a transverse field and coupled to an Ohmic bath of quantum harmonic oscillators. We analyze the coupled system using Monte Carlo simulations of the classical two-dimensional counterpart model. The coupling to the bath enhances the extent of the ordered phase, as found in mean-field spin-glasses. In the case of finite clusters we show that a generalization of the Caldeira-Leggett localization transition exists. In the case of the infinite random chain we study the effect of dissipation on the transition and the Griffiths phase.Comment: 21 pages, 10 figure

Influence of the Ni-Co/Al-Mg catalyst loading in the continuous aqueous phase reforming of the bio-oil aqueous fraction

The effect of catalyst loading in the Aqueous Phase Reforming (APR) of bio-oil aqueous fraction has been studied with a Ni-Co/Al-Mg coprecipitated catalyst. Because of the high content of water in the bio-oil aqueous fraction, APR could be a useful process to convert this fraction into valuable products. Experiments of APR with continuous feeding of aqueous solution of acetol, butanol and acetic acid as the only compound, together with a simulated and a real aqueous fraction of bio-oil, were carried out. Liquid products in the liquid effluent of the APR model compounds were quantified and the reaction pathways were revised. The increase of catalyst loading produced an increase of gas production and a gas with higher alkanes content. Acetol was the compound with the highest reactivity while the conversion of acetic acid was very low. The presence of acetic acid in the feed caused catalyst deactivation

Depositional fluxes and concentrations of \u3csup\u3e7\u3c/sup\u3eBe and \u3csup\u3e210\u3c/sup\u3ePb in bulk precipitation and aerosols at the interface of Atlantic and Mediterranean coasts in Spain

Bulk depositional fluxes of 7Be and 210Pb in precipitation measured over a period of 16 months (April 2009–July 2010) in Huelva, Spain varied between 5.6 and 186 Bq m−2 month−1 (annual mean: 834 Bq m−2 year−1) and 0.8 and 8.1 Bq m−2 month−1 (annual mean: 59 Bq m−2 year−1), respectively, with the lowest depositional fluxes occurring during dry summer months. Quantitative evaluation of the precipitation-normalized seasonal depositional fluxes of 7Be and 210Pb indicates that the enrichment factor in winter is \u3c 1.0 while in 2010 spring, it is significantly higher than 1, possibly indicating input of air from the stratosphere-troposphere exchange (for 7Be). The specific activities of 7Be and 210Pb varied from 0.03 to 7.42 Bq L−1 (mean = 2.5 Bq L−1) and 0.005 to 1.07 BqL−1 (mean = 0.23 Bq L−1), respectively, with the highest values corresponding to the spring season. The spatial and temporal variations of 7Be and 210Pb in aerosols from three stations are evaluated and compared to their monthly depositional fluxes. The mean depositional velocity of aerosols using 7Be and 210Pb are similar, ∼0.5 cm s−1 and are compared to other published values. This is the first time the fractional amounts of 7Be and 210Pb in monthly bulk precipitation are compared to the fractional amount of precipitation and provides insight on how the amount of precipitation plays a key role on the scavenging of these nuclides. The importance of dry fallout is evaluated for the study site which has direct implications for other areas in the Mediterranean Climate Zone

European wildcat populations are subdivided into five main biogeographic groups: consequences of Pleistocene climate changes or recent anthropogenic fragmentation?

Extant populations of the European wildcat are fragmented across the continent, the likely consequence of recent extirpations due to habitat loss and over-hunting. However, their underlying phylogeographic history has never been reconstructed. For testing the hypothesis that the European wildcat survived the Ice Age fragmented in Mediterranean refuges, we assayed the genetic variation at 31 microsatellites in 668 presumptive European wildcats sampled in 15 European countries. Moreover, to evaluate the extent of subspecies/population divergence and identify eventual wild × domestic cat hybrids, we genotyped 26 African wildcats from Sardinia and North Africa and 294 random-bred domestic cats. Results of multivariate analyses and Bayesian clustering confirmed that the European wild and the domestic cats (plus the African wildcats) belong to two well-differentiated clusters (average Ф ST = 0.159, r st = 0.392, P > 0.001; Analysis of molecular variance [AMOVA]). We identified from c. 5% to 10% cryptic hybrids in southern and central European populations. In contrast, wild-living cats in Hungary and Scotland showed deep signatures of genetic admixture and introgression with domestic cats. The European wildcats are subdivided into five main genetic clusters (average Ф ST = 0.103, r st = 0.143, P > 0.001; AMOVA) corresponding to five biogeographic groups, respectively, distributed in the Iberian Peninsula, central Europe, central Germany, Italian Peninsula and the island of Sicily, and in north-eastern Italy and northern Balkan regions (Dinaric Alps). Approximate Bayesian Computation simulations supported late Pleistocene-early Holocene population splittings (from c. 60 k to 10 k years ago), contemporary to the last Ice Age climatic changes. These results provide evidences for wildcat Mediterranean refuges in southwestern Europe, but the evolution history of eastern wildcat populations remains to be clarified. Historical genetic subdivisions suggest conservation strategies aimed at enhancing gene flow through the restoration of ecological corridors within each biogeographic units. Concomitantly, the risk of hybridization with free-ranging domestic cats along corridor edges should be carefully monitored