Abundance and distribution of California sea lions (Zalophus californianus) in central and northern California during 1998 and summer 1999

The abundance and distribution of California sea lions (Zalophus californianus) in central and northern California was studied to allow future evaluation of their impact on salmonids, the ecosystem, and f isheries. Abundance at-sea was estimated by using the strip transect method from a fixed-wing aircraft with a belly viewing port. Abundance on land was estimated from 126-mm-format aerial photographs of animals at haulouts between Point Conception and the California−Oregon border. The sum of these two estimates represented total abundance for central and northern California. Both types of survey were conducted in May−June 1998, September 1998, December 1998, and July 1999. A haulout survey was conducted in July 1998. The greatest number of sea lions occurred near Monterey Bay and San Francisco Bay for all surveys. Abundance was high in central and northern California in 1998 when warm water from the 1997−98 El Niño affected the region and was low in July 1999 when cold water La Niña conditions were prevalent. At-sea abundance estimates in central and northern California ranged from 12,232 to 40,161 animals, and haulout abundance was 13,559 to 36,576 animals. Total abundance of California sea lions in central and northern California was estimated as 64,916 in May−June 1998, 75,673 in September 1998, 56,775 in December 1998, and 25,791 in July 1999. The proportion of total abundance to animals hauled-out for the four complete surveys ranged from 1.77 to 2.13, and the mean of 1.89 was used to estimate a total abundance of 49,697 for July 1998. This multiplier may be applicable in the future to estimate total abundance of California sea lions off central and northern California if only the abundance of animals at haulout sites is known

Organic Cation Transporter 3: A Cellular Mechanism Underlying Rapid, Non-Genomic Glucocorticoid Regulation of Monoaminergic Neurotransmission, Physiology, and Behavior

Corticosteroid hormones act at intracellular glucocorticoid receptors (GR) and mineralocorticoid receptors (MR) to alter gene expression, leading to diverse physiological and behavioral responses. In addition to these classical genomic effects, corticosteroid hormones also exert rapid actions on physiology and behavior through a variety of non-genomic mechanisms, some of which involve GR or MR, and others of which are independent of these receptors. One such GR-independent mechanism involves corticosteroid-induced inhibition of monoamine transport mediated by “uptake2” transporters, including organic cation transporter 3 (OCT3), a low-affinity, high-capacity transporter for norepinephrine, epinephrine, dopamine, serotonin and histamine. Corticosterone directly and acutely inhibits OCT3-mediated transport. This review describes the studies that initially characterized uptake2 processes in peripheral tissues, and outlines studies that demonstrated OCT3 expression and corticosterone-sensitive monoamine transport in the brain. Evidence is presented supporting the hypothesis that corticosterone can exert rapid, GR-independent actions on neuronal physiology and behavior by inhibiting OCT3-mediated monoamine clearance. Implications of this mechanism for glucocorticoid-monoamine interactions in the context-dependent regulation of behavior are discussed

Mechanics of liquid helium in a partially filled rotating dewar in low gravity with application to Gravity Probe-B

The Gravity Probe-B spacecraft is composed largely of a liquid helium dewar containing an experiment package. It is shown that an unsymmetric liquid helium distribution in the dewar can cause unacceptably high forces, gravitational and gravity gradient forces, at the experiment location. It is further shown that for the planned spacecraft configuration and operational parameters, it is very likely that the liquid helium distribution in the dewar will be unsymmetric. The required symmetry can be attained by using higher operational spacecraft rotation rates

Identification of the YfgF MASE1 domain as a modulator of bacterial responses to aspartate

Complex 3'-5'-cyclic diguanylic acid (c-di-GMP) responsive regulatory networks that are modulated by the action of multiple diguanylate cyclases (DGC; GGDEF domain proteins) and phosphodiesterases (PDE; EAL domain proteins) have evolved in many bacteria. YfgF proteins possess a membrane-anchoring domain (MASE1), a catalytically inactive GGDEF domain and a catalytically active EAL domain. Here, sustained expression of the Salmonella enterica spp. Enterica ser. Enteritidis YfgF protein is shown to mediate inhibition of the formation of the aspartate chemotactic ring on motility agar under aerobic conditions. This phenomenon was c-di-GMP-independent because it occurred in a Salmonella strain that lacked the ability to synthesize c-di-GMP and also when PDE activity was abolished by site-directed mutagenesis of the EAL domain. YfgF-mediated inhibition of aspartate chemotactic ring formation was impaired in the altered redox environment generated by exogenous p-benzoquinone. This ability of YfgF to inhibit the response to aspartate required a motif, (213)Lys-Lys-Glu(215), in the predicted cytoplasmic loop between trans-membrane regions 5 and 6 of the MASE1 domain. Thus, for the first time the function of a MASE1 domain as a redox-responsive regulator of bacterial responses to aspartate has been shown

Space shuttle main engine high pressure fuel pump aft platform seal cavity flow analysis

A general purpose, three-dimensional computational fluid dynamics code named PHOENICS, developed by CHAM Inc., is used to model the flow in the aft-platform seal cavity in the high pressure fuel pump of the space shuttle main engine. The model is used to predict the temperatures, velocities, and pressures in the cavity for six different sets of boundary conditions. The results are presented as input for further analysis of two known problems in the region, specifically: erratic pressures and temperatures in the adjacent coolant liner cavity and cracks in the blade shanks near the outer diameter of the aft-platform seal

Photometry of cometary nuclei: Rotation rates, colours and a comparison with Kuiper Belt Objects

We present time-series data on Jupiter Family Comets (JFCs) 17P/Holmes, 47P/Ashbrook-Jackson and 137P/Shoemaker-Levy 2. In addition we also present results from `snap-shot' observations of comets 43P/Wolf-Harrington, 44P/Reinmuth 2, 103P/Hartley 2 and 104P/Kowal 2 taken during the same run. The comets were at heliocentric distances of between 3 and 7 AU at this time. We present measurements of size and activity levels for the snap-shot targets. The time-series data allow us to constrain rotation periods and shapes, and thus bulk densities. We also measure colour indices (V-R) and (R-I) and reliable radii for these comets. We compare all of our findings to date with similar results for other comets and Kuiper Belt Objects (KBOs). We find that the rotational properties of nuclei and KBOs are very similar, that there is evidence for a cut-off in bulk densities at ~ 0.6 g cm^{-3} in both populations, and the colours of the two populations show similar correlations. For JFCs there is no observational evidence for the optical colours being dependant on either position in the orbit or on orbital parameters.Comment: 15 pages, 19 figures, accepted for publication in MNRA

Shuttle Ground Operations Efficiencies/Technologies Study (SGOE/T). Volume 5: Technical Information Sheets (TIS)

The Technology Information Sheet was assembled in database format during Phase I. This document was designed to provide a repository for information pertaining to 144 Operations and Maintenance Instructions (OMI) controlled operations in the Orbiter Processing Facility (OPF), Vehicle Assembly Building (VAB), and PAD. It provides a way to accumulate information about required crew sizes, operations task time duration (serial and/or parallel), special Ground Support Equipment (GSE). required, and identification of a potential application of existing technology or the need for the development of a new technolgoy item