The development of a spatial decision support system to optimise agricultural resource use in the Western Cape

This paper describes the development of a decision support model for regional agricultural resource utilisation. The analysis was generated in a spatial context and the optimisation technique was interactive with a geographical information system (GIS). Economic and operational research methodologies were linked to the GIS in the process of determining the appropriate resource uses for the region. The optimisation technique was applied for the Western Cape Province for eight crops. The results of this research are discussed in this paper, with specific reference to its application value for the public sector and agri-business.Farm Management, Resource /Energy Economics and Policy,

Primary health care in the South African context – medical students\' perspectives

Background Both the South African Department of Health and the University of Cape Town (UCT) have committed to the primary health care (PHC) approach, which is best captured in the Declaration of Alma Ata. If medical students are to be trained in the PHC approach, it is important that they not only have a good understanding of this approach, but are also aware of the social, economic and political context that they will be working in when they have qualified, so that they can develop realistic expectations of their careers as doctors. From research that was conducted at UCT, this article covers UCT medical students' views of health care in South Africa, including their perceptions of the applicability and implementation of the PHC approach for South Africa, and their perceptions of how the South African government features in issues of health and the PHC approach. Methods Mixed-methods were used, but this article will focus on the qualitative data gathered. One hundred and seventeen medical students (years one to four) were purposively selected to be involved in focus groups and interviews. These focus groups were conducted between February 2004 and March 2005. Results Students acknowledged that the state of health care in South Africa needs to change and showed an awareness of the role that South Africa's history of apartheid has played in the state of health care in these areas and the existence of inequity. They however did not agree on the applicability of the PHC approach to the South African situation. The PHC approach is seen not to be working in South Africa because of various obstacles to its implementation and success, such as disorganisation within the health system, and a lack of infrastructure, finances and resources. There seemed to be a general understanding amongst the students that they will have been trained in the PHC approach but then will be working within a system that has possibly not undergone similar changes. Students agreed on the important role of government in PHC, some maintaining that the government should be at the forefront of its implementation, but were generally dissatisfied with the role the South African government is currently playing in health care. Conclusion It is encouraging that students are generally aware of the reality of health care in South Africa and of the fact that more change needs to take place. However, it may be that many students who have a limited understanding of the impact that apartheid had on health care provision in South Africa, and this could then impact on students' perceptions of the applicability of the current PHC approach for South Africa. Students' views that the PHC approach has not been fully implemented in South Africa are a concern, as it is these types of views that are likely to cause students to lose confidence in the PHC approach, and will most likely widen the disjuncture between theory of the approach and the reality of its implementation. Regarding the political dynamics of the PHC approach, students do need to be aware of political factors that can impact on the success of this approach. Discussion around and research into the social, economic and political context of health care and medical education has particular relevance for South Africa, and it is vital that students' views on these issues are acknowledged so that areas for change can be identified and addressed. South African Family Practice Vol. 49 (10) 2007: pp. 6-1

Primary health care in the South African context - medical students' perspectives

Background Both the South African Department of Health and the University of Cape Town (UCT) have committed to the primary health care (PHC) approach, which is best captured in the Declaration of Alma Ata. If medical students are to be trained in the PHC approach, it is important that they not only have a good understanding of this approach, but are also aware of the social, economic and political context that they will be working in when they have qualified, so that they can develop realistic expectations of their careers as doctors. From research that was conducted at UCT, this article covers UCT medical students' views of health care in South Africa, including their perceptions of the applicability and implementation of the PHC approach for South Africa, and their perceptions of how the South African government features in issues of health and the PHC approach. Methods Mixed-methods were used, but this article will focus on the qualitative data gathered. One hundred and seventeen medical students (years one to four) were purposively selected to be involved in focus groups and interviews. These focus groups were conducted between February 2004 and March 2005. Results Students acknowledged that the state of health care in South Africa needs to change and showed an awareness of the role that South Africa's history of apartheid has played in the state of health care in these areas and the existence of inequity. They however did not agree on the applicability of the PHC approach to the South African situation. The PHC approach is seen not to be working in South Africa because of various obstacles to its implementation and success, such as disorganisation within the health system, and a lack of infrastructure, finances and resources. There seemed to be a general understanding amongst the students that they will have been trained in the PHC approach but then will be working within a system that has possibly not undergone similar changes. Students agreed on the important role of government in PHC, some maintaining that the government should be at the forefront of its implementation, but were generally dissatisfied with the role the South African government is currently playing in health care. Conclusion It is encouraging that students are generally aware of the reality of health care in South Africa and of the fact that more change needs to take place. However, it may be that many students who have a limited understanding of the impact that apartheid had on health care provision in South Africa, and this could then impact on students' perceptions of the applicability of the current PHC approach for South Africa. Students' views that the PHC approach has not been fully implemented in South Africa are a concern, as it is these types of views that are likely to cause students to lose confidence in the PHC approach, and will most likely widen the disjuncture between theory of the approach and the reality of its implementation. Regarding the political dynamics of the PHC approach, students do need to be aware of political factors that can impact on the success of this approach. Discussion around and research into the social, economic and political context of health care and medical education has particular relevance for South Africa, and it is vital that students' views on these issues are acknowledged so that areas for change can be identified and addressed

Medical students' attitudes towards the primary healthcare approach - what are they and how do they change?

Background: The context of the research presented in this article is the new MBChB curriculum at the University of Cape Town (UCT) that has been in operation since 2002. This new curriculum is primary health care (PHC) driven and puts emphasis on the integration of biological and psychosocial elements. The context of curriculum reform at UCT can be placed within the broader South African context, in which the South African Department of Health has made a commitment to the PHC approach. The aim of this research was to provide an understanding of medical students’ attitudes towards the PHC approach. The findings presented in this article form part of a broader set of findings for a PhD research study aimed at qualitatively exploring medical students’ attitudes towards and perceptions of PHC. Methods: A qualitative approach was used and focus groups and interviews were conducted with second-, third- and fourth year medical students at UCT. A total of 82 students were purposively selected to participate in the research. A content analytic approach was used to analyse the focus group and interview data. Results: The students generally had a positive attitude towards the PHC approach and were positive about UCT’s decision to promote this approach. Some, however, were concerned about the international relevance and status of their degree, and concerns were also raised about the contrast between the theory and reality of the approach, with many labelling PHC as idealistic. The students’ responses indicated that their attitudes towards the PHC approach were open to change during the course of their academic career and were influenced by a range of factors. Some of these factors are related to the medical school environment, such as the PHC approach itself, how PHC is taught, and the views of other students and staff at UCT. Other factors that were not related to the university included personality, the students’ background and exposure to health facilities, and clinical exposure outside UCT. Conclusion: These findings raise the question of whether students are able to think and feel positively about the PHC approach, but not actually internalise the philosophy of the approach. The students’ struggle with the incongruence between what is perceived as the idealistic theory of PHC and the reality of health care in South Africa is also an issue that needs to be acknowledged. These issues have international relevance, and are particularly significant in South Africa, where a commitment has been made by the South African Department of Health to the PHC approach and where doctors are set to play a vital role in its implementation and success

Validation of the CoaguChek XS international normalised ratio point-of-care analyser in patients at Charlotte Maxeke Johannesburg Academic Hospital, South Africa

Background. Measurement of the international normalised ratio (INR) is essential in the management of patients on long-term warfarin therapy. The CoaguChek XS portable coagulometer is a point-of-care test for INR measurement. It offers the advantage of improved patient accessibility, particularly in peripheral clinics.Objectives. To evaluate the clinical utility of the CoaguChek XS for monitoring of patients on standard warfarin therapy (INR 2 - 3) as well as those with mechanical heart valve replacements (INR 2.5 - 3.5).Methods. We compared the performance of the CoaguChek XS device with that of the STAGO laboratory analyser with regard to accuracy and precision in 304 patients referred for routine testing.Results. The mean INR value of the CoaguChek XS of 2.75 (standard deviation (SD) 1.18) was comparable to that of the STAGO (2.65 (SD 1.04)). The Bland Altman difference plot revealed good agreement. Bias between the two methods was small, and the imprecision was within acceptable limits. Within the target range (2.0 - 3.5), 93.9% of the CoaguChek XS INR readings were within 0.5 units of the standard laboratory method result. There was, however, an increase in the variability of the differences between the two test methods when the INR was >3.6.Conclusion. The CoaguChek XS point-of-care device can be used to provide accurate and precise INR measurements over a wide range for monitoring of valvular and non-valvular patients on long-term warfarin therapy

Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ

AIM To identify and characterize the effect of phosphorylation on the subcellular localization of Ankrd54. METHODS HEK293T cells were treated with calyculin A, staurosporin or phorbol 12-myristate 13-acetate (PMA). Cells were transfected with eGFP-tagged Ankrd54 with or without Lyn tyrosine kinase (wild-type, Y397F mutant, or Y508F mutant). The subcellular localization was assessed by immunofluorescence imaging of cells, immunoblotting of subcellular fractionations. The phosphorylation of Ankrd54 was monitored using Phos-tagTM gel retardation. Phosphorylated peptides were analysed by multiple-reaction-monitoring (MRM) proteomic analysis. RESULTS Activation of PKC kinases using PMA promoted nuclear export of Ankrd54 and correlated with increased Ankrd54 phosphorylation, assayed using Phos-tagTM gel retardation. Co-expression of an active form of the PKCδ isoform specifically promoted both phosphorylation and cytoplasmic localization of Ankrd54, while PKCδ, Akt and PKA did not. Alanine mutation of several serine residues in the amino-terminal region of Ankrd54 (Ser14, Ser17, Ser18, Ser19) reduced both PMA induced cytoplasmic localization and phosphorylation of Ankrd54. Using MRM proteomic analysis, phosphorylation of the Ser18 residue of Ankrd54 was readily detectable in response to PMA stimulation. PMA stimulation of cells co-expressing Ankrd54 and Lyn tyrosine kinase displayed increased co-immunoprecipitation and enhanced co-localization in the cytoplasm. CONCLUSION We identify phosphorylation by PKCδ as a major regulator of nuclear-cytoplasmic shuttling of Ankrd54, and its interaction with the tyrosine kinase Lyn

Slant cues are processed with different latencies for the online control of movement

For the online control of movement, it is important to respond fast. The extent to which cues are effective in guiding our actions might therefore depend on how quickly they provide new information. We compared the latency to alter a movement when monocular and binocular cues indicated that the surface slant had changed. We found that subjects adjusted their movement in response to three types of information: information about the new slant from the monocular image, information about the new slant from binocular disparity, and information about the change in slant from the change in the monocular image. Responses to changes in the monocular image were approximately 40 ms faster than responses to a new slant estimate from binocular disparity and about 90 ms faster than responses to a new slant estimate from the monocular image. Considering these delays, adjustments of ongoing movements to changes in slant will usually be initiated by changes in the monocular image. The response will later be refined on the basis of combined binocular and monocular estimates of slant. © ARVO

Perbanyakan Mikro Colocasia Esculenta (L.) Schott Var. Antiquorum melalui Penggunaan IAA

Media perbanyakan in vitrosangat diperlukan untuk meningkatkan kemampuan multipikasi tunas maupun kualitas bibit. Media Murashige Shoog (MS)dapatditambahkanzat pengatur tumbuh sebagai pemacu pertumbuhan dalam kultur in vitro.Penelitian ini bertujuan untuk mendapatkan konsentrasi IAA terbaik bagi pertumbuhan talas jepang dalam kulturin vitro. Perlakuan terdiri dari 4 taraf konsentrasi IAA yaitu I0 (0 mg/L), I1 (0,5 mg/L), I2 (1 mg/L), I3 (1,5 mg/L) yang diulang sebanyak 5 kali dalam rancangan acak kelompok. Hasil penelitian ini menunjukan bahwa pemberian IAA berpengaruh nyata terhadap saat muncul tunas, jumlah tunas, tinggi tunas, jumlah daun dan jumlah akar satoimo. Konsentrasi IAA 0,5 mg/L merupakan konsentrasi terbaik untuk pertumbuhan akar dan saat muncul tunas talas satoimo, sedangkan konsentrasi IAA 1 mg/L merupakan konsentrasi terbaik untuk jumlah tunas, tinggi tunas dan jumlah daun satoimo. Kata kunci: kultur in vitro, talas jepang, IAA, perbanyaka

The Occurrence and Infectivity of Arbuscular Mycorrhizal Fungi in Inoculated and Uninoculated Rhizosphere Soils of Two-year-old Commercial Grapevines

Arbuscular mycorrhizal (AM) fungal populations present in the rhizosphere of vine roots in the vineyards of a commercial farm in the Stellenbosch Region were investigated using microscopic analyses. AM root colonisation levels of between 70% and 90% were found in both grapevine roots that were previously artificially inoculated with commercial AM inocula, and in uninoculated (control) grapevine roots. The AM fungal isolates in the rhizosphere soil, identified using morphological criteria, belonged to the genera Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora.  The majority of species found was not present in the commercial inocula and was either indigenous to the vineyard or originated from the nursery where the vines were obtained. Isolates of Glomus and Acaulospora appeared to be the most abundant. The AM fungal species occurred at a soil phosphorus (P) concentration of up to 80 mg/kg P and a soil pH (KCl) that ranged between 5.63 and 6.10. Total spore counts ranged between 1 000 and 3 779 spores/100 g dry soil. In accordance with literature, lower spore concentrations were recorded for the heavier soil types with no cover crop system, compared with the sandy soil type on which cover crops were sown annually

Prevalence of musculoskeletal injuries among adolescent squash players in the Western Cape

Objective. To determine the prevalence of musculoskeletal injuries among adolescent squash players in the Western Cape. Design. A retrospective descriptive survey was conducted during the first week of May 2006. Three schools were randomly selected from a list of the top 10 schools in the Western Cape high school squash league of 2005, with 106 squash players aged 13 - 18 years participating in the study. Setting. Injury data were collected for 106 players at three schools randomly selected from a list of the top 10 schools in the Western Cape high school squash league of 2005. Interventions. An adapted structured self-administered questionnaire based on a previously validated musculoskeletal injury questionnaire was used to collect the data. Main outcome measures. The main variables investigated were prevalence, mechanism and injury site of musculoskeletal squash injuries. Results. Twenty-nine per cent of the players (N = 31) reported that they had sustained a squash injury in the 4 weeks prior to data collection. A total of 48 injuries were reported by the injured players. The most common injuries included those of the thigh (19%), shoulder (13%) and lower back (13%). Forty-two per cent of players reported no specific mechanism of injury, but experienced pain not associated with a traumatic injury only while playing squash. Conclusion. A relatively high prevalence of squash injuries was found. This preliminary study serves as a baseline for future research. Areas for further investigation were identified and this could lead to the implementation of preventive programmes and education to prevent injuries among adolescent squash players. South African Journal of Sports Medicine Vol. 19 (1) 2007: pp. 3-