20 research outputs found
Molecular identification of zoonotic and livestock-specific Giardia-species in faecal samples of calves in Southern Germany
What is adaptive clothing and how can it make life easier for people with a disability?
Have you ever tried to do up a zip or button a shirt one-handed? Put on a pair of jeans while seated? Do you know someone with Autism Spectrum Disorder, who canât stand the feeling of certain fabrics against their skin? If your feet are different sizes, or you only have one foot, how do you buy shoes? Advances in âadaptive clothingâ aim to address these problems. Adaptive clothes are specially designed for people with a disability. This can mean providing one-handed zippers on shoes, replacing buttons with magnetic closures or designing clothing and footwear so you can get dressed while in a seated position. The key to effective adaptive clothing is catering for the vast array of needs different consumers have, while maintaining style and fashionability. Recently, fashion brands have begun to provide on-trend clothing with new styles, combining fashion and technology for people with a variety of disabilities
Molecular identification of zoonotic and livestock-specific Giardia-species in faecal samples of calves in Southern Germany
Background: Giardia-infection in cattle is often subclinical or asymptomatic, but it can also cause diarrhoea. The livestock-specific species Giardia bovis is the most frequently observed in cattle, however, the two zoonotic species Giardia duodenalis and Giardia enterica have also been found. Therefore calves are thought to be of public health significance. The aim of this study was to obtain current data about the frequency of the different Giardia-species in calves in Southern Germany. Findings: Faecal samples of calves (diarrhoeic and healthy) in Southern Germany, diagnosed Giardia-positive by microscopy, were characterised by multi-locus PCR and sequencing. Of 152 microscopically Giardia-positive samples 110 (72.4%) were positive by PCR and successfully sequenced. G. bovis (Assemblage E) was detected in 101/110 (91.8%) PCR-positive samples, whilst G. duodenalis (Assemblage A) was detected in 8/110 (7.3%) samples and a mixed infection with G. duodenalis and G. bovis (Assemblage A+E) was identified in 1/110 (0.9%) samples. The sub-genotypes A1, E2 and E3 were identified with the beta-giardin and the glutamate dehydrogenase genes. In the majority of diarrhoeic faecal samples a co-infection with Cryptosporidium spp. or Eimeria spp. was present, however, there were some in which G. bovis was the only protozoan pathogen found. Conclusions: The results suggest that there is potentially a risk for animal handlers as calves in Southern Germany are, at a low percentage, infected with the zoonotic species G. duodenalis. In addition, it was found that G. bovis was the only pathogen identified in some samples of diarrhoeic calves, indicating that this parasite may be a contributing factor to diarrhoea in calves
Further validation of the Health Promoting Activities Scale with mothers of typically developing children
Background/aim: The Health Promoting Activities Scale (HPAS) measures the frequency that mothers participate in selfâselected leisure activities that promote health and wellbeing. The scale was originally validated on mothers of schoolâaged children with disabilities, and the current article extends this research using a comparative sample of mothers of typically developing schoolâaged children.
Method: Australian mothers (N = 263) completed a questionnaire containing the HPAS, a measure of depression, anxiety and stress (DASSâ21) and questions concerning their weight, height, sleep quality and demographics. Statistical analysis assessed the underlying structure, internal consistency and construct validity of the HPAS. Inferential statistics were utilised to investigate the construct validity.
Results: Exploratory factor analysis supported the unidimensionality of the HPAS. It showed good internal consistency (Cronbach's alpha = 0.78). Significantly lower HPAS scores were recorded for women who were obese; had elevated levels of depression, anxiety and stress; had poor quality sleep or had heavy caring commitments. The mean HPAS score in this sample (M = 32.2) was significantly higher than was previously reported for women of children with a disability (M = 21.6: P < 0.001).
Conclusions: Further psychometric evaluation of the HPAS continues to support the HPAS as a sound instrument that measures the frequency that women participate in meaningful occupation that is associated with differences in mental health and wellbeing and other health indicators
COVID-19 has changed the future of retail: thereâs plenty more automation in store
Australian supermarket giant Woolworths has announced its single biggest investment in logistics infrastructure, spending A$780 million to replace up to 1,300 workers with robots.It plans to build one semi-automated and one fully automated distribution centre in south-west Sydney. About 650 jobs will be created at the new centres, to open in 2024. Three existing centres (two in Sydney, one in Melbourne) will close as a result
Investigation of the self reported health and health related behaviours of Victorian mothers of school aged children
Adjustment of mothers of children with obstetrical brachial plexus injuries: testing a risk and resistance model
Objective: To investigate direct and moderating effects of risk and resistance factors on the adjustment of mothers of children with obstetrical brachial plexus injuries (OBPI). Participants: Fifty-three mothers of children with OBPI recruited from an OBPI clinic in an Australian pediatric hospital. Measures: OBPI Severity Scale, Parents of Children With Disabilities Inventory, Family APGAR, Short Form Social Support Questionnaire-6, Life Orientation Test-Revised, Perceived Control of Internal States Scale, General Health Questionnaire-12. Results: The factors explained 30% of maternal adjustment variation. Resistance factors explained a unique 18%. Optimism was the only significant moderator. Conclusions: Findings confirm the importance of risk and resistance factors in maternal adjustment and enhance understanding by identifying optimism as a moderator of the risk/adjustment relationship. Interventions promoting optimism may facilitate maternal adjustment
Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia
In Western Australia a number of indigenous Trypanosoma spp. infect susceptible native marsupials, such as the woylie (Bettongia penicillata), brushtail possum (Trichosurus vulpecula), and chuditch (Dasyurus geoffroii). Two genotypes of Trypanosoma copemani (identified as G1 and G2) have been found in the woylie, and G2 has been implicated in the decline of this host species, making its presence of particular interest. Here we used targeted amplicon next generation sequencing (NGS) of the Trypanosoma 18S rDNA loci on 70 Trypanosoma-positive marsupial blood samples, to identify T. copemani genotypes and multiple Trypanosoma infections (polyparasitism) in woylies and cohabiting species in Western Australia. Polyparasitism with Trypanosoma spp. was found in 50% of the wildlife sampled, and within species diversity was high, with 85 zero-radius operational taxonomic units (ZOTUs) identified in nine putative parasite species. Trypanosoma copemani was assigned 17 ZOTUs and was identified in 80% of samples. The most abundant ZOTU isolated (63%) differed slightly from the published genotype of G1, and G2 was the second most abundant ZOTU (14%). Trypanosome diversity was significantly greater in woylies than in brushtail possums, and parasite community composition also differed significantly between these host species. One novel Trypanosoma spp. genotype (Trypanosoma sp. ANU2) was found in 20% of samples. A species of Crithidia was detected in a woylie, and two avian trypanosomes (Trypanosoma avium and Trypanosoma sp. AAT) were identified in woylies for the first time. Keywords: Trypanosoma spp., Bettongia penicillata, Trichosurus vulpecula, Host-parasite relationship, 18S rDNA targeted amplicon sequencin
Increased Trypanosoma spp. richness and prevalence of haemoparasite co-infection following translocation
Abstract Background Understanding how fauna translocation and antiparasitic drug treatment impact parasite community structure within a host is vital for optimising translocation outcomes. Trypanosoma spp. and piroplasms (Babesia and Theileria spp.) are known to infect Australian marsupials, including the woylie (Bettongia penicillata). However relatively little is known about these haemoparasites, or how they respond to management practices such as translocation. We monitored haemoparasites infecting woylies for up to 12Â months during two fauna translocations to supplement existing woylie populations in three different sites (Dryandra, Walcott and Warrup East) within south-western Australia between 2014 and 2016, with the aim of investigating (i) how haemoparasite prevalence, Trypanosoma spp. richness and Trypanosoma spp. community composition varied over time and between different sites following translocation; and (ii) whether ivermectin treatment indirectly impacts haemoparasite prevalence. Using molecular methods, 1211 blood samples were screened for the presence of trypanosomes, and a subset of these samples (nâ=â264) were also tested for piroplasms. Results Trypanosomes and piroplasms were identified in 55% and 94% of blood samples, respectively. We identified five Trypanosoma species, two Theileria species, a single species of Babesia and a novel Bodo species. Trypanosoma spp. richness and the prevalence of haemoparasite co-infection increased after translocation. Prior to translocation, Trypanosoma spp. community composition differed significantly between translocated and resident woylies within Walcott and Warrup East, but not Dryandra. Six months later, there was a significant difference between translocated and resident woylies within Dryandra, but not Walcott or Warrup East. The response of haemoparasites to translocation was highly site-specific, with predominant changes to the haemoparasite community in translocated woylies occurring within the first few months following translocation. Ivermectin treatment had no significant effect on haemoparasite prevalence. Conclusions This study contributes to our understanding of haemoparasite dynamics in woylies following translocation. The highly site-specific and rapid response of haemoparasites to translocation highlights the need to better understand what drives these effects. Given that haemoparasite prevalence and composition of translocated and resident animals changed significantly following translocation, we propose that parasite monitoring should form an essential component of translocation protocols, and such protocols should endeavour to monitor translocated hosts and cohabiting species