Transcriptomic fingerprinting of Pseudomonas putida under alternative physiological regimes

Pseudomonas putida KT2440 is a metabolically versatile soil bacterium useful both as a model biodegradative organism and as a host of catalytic activities of biotechnological interest. In this report, we present the high-resolution transcriptome of P. putida cultured on different carbon sources as revealed by deep sequencing of the corresponding RNA pools. Examination of the data from growth on substrates that are processed through distinct pathways (glucose, fructose, succinate and glycerol) revealed that > 20% of the P. putida genome is differentially expressed depending on the ensuing physiological regime. Changes affected not only metabolic genes but also a suite of global regulators e.g. the rpoS sigma subunit of RNAP, various cold-shock proteins and the three HU histone-like proteins. Specifically, the genes encoding HU subunit variants hupA, hupB and hupN drastically altered their expression levels (and thus their ability to form heterodimeric combinations) under the diverse growth conditions. Furthermore, we found that two small RNAs, crcZ and crcY, known to inhibit the Crc protein that mediates catabolite repression in P. putida, were both down-regulated by glucose. The raw transcriptomic data generated in this work is made available to the community through the Gene Expression Omnibus database.This work was supported by the BIO and FEDER CONSOLIDER-INGENIO programs of the Spanish Ministry of Economy and Competitiveness, the MICROME, ST-FLOW and ARISYS Contracts of the EU, the ERANET Program and funding from the Autonomous Community of Madrid (PROMPT).Peer reviewe

Logros y retos de la política europea de cooperación internacional para el desarollo

Si bien la Unión Europea (UE), debido a su importante contribución a la cooperación internacional para el desarrollo es considerada la principal potencia solidaria del planeta, aún enfrenta importantes retos en la materia. Un ejemplo de ello es la incapacidad europea de instrumentar estrategias suficientemente coherentes respecto a otras acciones de influencia exterior, tales como la ayuda, en compatibilidad con la seguridad, el comercio y la migración. El artículo argumenta que, con todo y sus avances, la UE debe conseguir mejores niveles de coordinación en temas clave de su acción exterior, si lo que desea es constituirse como un actor consolidado y eficaz de cooperación para el desarrollo.Palavras-chave: cooperação internacional para o desenvolvimento, União Européia, coordenação

Logros y retos de la política europea de cooperación internacional para el desarollo

Si bien la Unión Europea (UE), debido a su importante contribución a la cooperación internacional para el desarrollo es considerada la principal potencia solidaria del planeta, aún enfrenta importantes retos en la materia. Un ejemplo de ello es la incapacidad europea de instrumentar estrategias suficientemente coherentes respecto a otras acciones de influencia exterior, tales como la ayuda, en compatibilidad con la seguridad, el comercio y la migración. El artículo argumenta que, con todo y sus avances, la UE debe conseguir mejores niveles de coordinación en temas clave de su acción exterior, si lo que desea es constituirse como un actor consolidado y eficaz de cooperación para el desarrollo.Palavras-chave: cooperação internacional para o desenvolvimento, União Européia, coordenação

Proyecto Arquitectónico Energéticamente Eficiente Mediante Gramáticas de Formas y Aprendizaje por Refuerzo

En este trabajo presentamos un sistema para la generación de esquemas de viviendas unifamiliares energéticamente eficientes. Los esquemas se sintetizan mediante la ejecución de gramáticas de formas simples, entrenadas por medio de técnicas de aprendizaje por refuerzo, teniendo en cuenta criterios tanto de habitabilidad como de eficiencia energética. Los resultados obtenidos son analizados y validadosUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Influencia de la geometría en los máximos de las tensiones en ajustes por interferencia con agujeros ranurados

Memoria D. Samuel Sol rzano Barruso” (USAL, Grant FS/21-2012 and Grant 463AC06).Elastic stress distributions in interference fits match the values predicted by pressure cylinders theory only if the two joined parts have the same length. In fact, a heavy stress concentration in the radial component appears at the edges of the contact zone being more intense in the case of the hub. Many methods have been proposed for relieving the stress peaks. In order to investigate how the stress concentrations are reduced, in this paper, different stress analysis in interference fits with grooved hubs were carried out using the finite element method.The authors wish to acknowledge the financial support provided by the Spanish Institution “Memoria D. Samuel Sol rzano Barruso” (USAL, Grant FS/21-2012 and Grant 463AC06)

Aproximación teórica a las tecnologías digitales de servicios públicos en territorios fronterizos y periféricos

[ES] El taller sobre Tecnologías Disruptivas de la Información y la Comunicación para la Innovación y la Transformación Digital, organizado en el ámbito del proyecto DISRUPTIVE (disruptive.usal.es) y celebrado el 12 de septiembre de 2022 en Valladolid, tiene como objetivo debatir sobre los problemas, retos y beneficios del uso de tecnologías digitales disruptivas, a saber, Internet de las Cosas, Big data, computación en la nube, sistemas multiagente, aprendizaje automático, realidad virtual y aumentada y robótica colaborativa, para apoyar la transformación digital en curso en la sociedad. El programa del taller incluyó 6 papers técnicos aceptados, 2 charlas de invitados y una sesión de networking. Este volumen contiene 6 de las ponencias presentadas en el taller sobre Tecnologías Disruptivas de la Información y la Comunicación para la Innovación y la Transformación Digital. Este taller fue organizado por ICE (Instituto para la Competitividad Empresarial de Castilla y León), UVa (Universidad de Valladolid) y apoyado principalmente por el Fondo Europeo de Desarrollo Regional (FEDER) a través del Programa Interreg España-Portugal V-A (POCTEP) bajo la subvención 0677_DISRUPTIVE_2_E (Dinamización de los Digital Innovation Hubs dentro de la región PocTep para el impulso de las TIC disruptivas y de última generación a través de la cooperación en la región transfronteriza).[EN] The workshop on Disruptive Information and Communication Technologies for Innovation and Digital transformation, organized under the scope of the DISRUPTIVE project (disruptive.usal.es) and held on September 12, 2022 in Valladolid, aims to discuss problems, challenges and benefits of using disruptive digital technologies, namely Internet of Things, Big data, cloud computing, multi-agent systems, machine learning, virtual and augmented reality, and collaborative robotics, to support the on-going digital transformation in society. The main topics included: Intelligent Manufacturing Systems; Industry 4.0 and digital transformation; Internet of Things; Cyber-security; Collaborative and intelligent robotics; Multi-Agent Systems; Industrial Cyber-Physical Systems; Virtualization and digital twins; Predictive maintenance; Virtual and augmented reality, Big Data and advanced data analytics; Edge and cloud Computing; Digital Transformation. The workshop program included 6 accepted technical papers, 2 invited talks and a networking session. This volume contains 6 of the papers presented at the Workshop on Disruptive Information and Communication Technologies for Innovation and Digital Transformation. This workshop was organized by ICE (Institute for Business Competitiveness of Castilla y León), UVa (University of Valladolid) and mainly supported by the European Regional Development Fund (ERDF) through the Interreg Spain-Portugal V-A Program (POCTEP) under grant 0677_DISRUPTIVE_2_E (Intensifying the activity of Digital Innovation Hubs within the PocTep region to boost the development of disruptive and last generation ICTs through cross-border cooperation)

Prognostic implications of troponin T variations in inherited cardiomyopathies using systems biology.

The cardiac troponin T variations have often been used as an example of the application of clinical genotyping for prognostication and risk stratification measures for the management of patients with a family history of sudden cardiac death or familial cardiomyopathy. Given the disparity in patient outcomes and therapy options, we investigated the impact of variations on the intermolecular interactions across the thin filament complex as an example of an unbiased systems biology method to better define clinical prognosis to aid future management options. We present a novel unbiased dynamic model to define and analyse the functional, structural and physico-chemical consequences of genetic variations among the troponins. This was subsequently integrated with clinical data from accessible global multi-centre systematic reviews of familial cardiomyopathy cases from 106 articles of the literature: 136 disease-causing variations pertaining to 981 global clinical cases. Troponin T variations showed distinct pathogenic hotspots for dilated and hypertrophic cardiomyopathies; considering the causes of cardiovascular death separately, there was a worse survival in terms of sudden cardiac death for patients with a variation at regions 90-129 and 130-179 when compared to amino acids 1-89 and 200-288. Our data support variations among 90-130 as being a hotspot for sudden cardiac death and the region 131-179 for heart failure death/transplantation outcomes wherein the most common phenotype was dilated cardiomyopathy. Survival analysis into regions of high risk (regions 90-129 and 130-180) and low risk (regions 1-89 and 200-288) was significant for sudden cardiac death (p = 0.011) and for heart failure death/transplant (p = 0.028). Our integrative genomic, structural, model from genotype to clinical data integration has implications for enhancing clinical genomics methodologies to improve risk stratification

Characterization of the distribution of water vapour for DInSAR studies on the volcanic island of Tenerife, Canary Islands

Measurements of ground displacement have been carried out on the entire active volcanic island of Tenerife, Canary Islands, by means of classical and advanced DInSAR techniques. The main limiting factor on the accuracy of DInSAR measurements is the distribution of the water vapour in the lower troposphere. Hence, it is yet necessary to perform a detailed spatial and temporal characterization of water vapour to understand, and to be able to carry out a direct computation of, the effect of the tropospheric delay on DInSAR results. In this sense, satellite and balloon data have been analysed to infer the variability in the distribution of water vapour and hence, the robustness of DInSAR results on the island of Tenerife.Peer reviewe

Physicochemical characterization of Crithmum maritimum L. and Daucus carota subsp. gummifer (Syme) Hook.fil. and their antimicrobial activity against apple tree and grapevine phytopathogens

Crithmum maritimum and Daucus carota subsp. gummifer are two species of the Apiaceae family that share multiple characteristics: both are halophitic, live on cliffs in the same geographic habitats, and are edible. While C. maritimum is rich in essential oils and flavonoids, D. carota is rich in terpenes and a gum producer. In the work presented herein, the biomass of these two wild plants and the bioactive compounds present in their extracts have been studied by elemental and thermal analysis, infrared spectroscopy, and gas chromatography-mass spectroscopy. To explore their bioactivities, both their hydroalcoholic extracts and their major constituents (apiole in C. maritimum and geranyl acetate in D. carota), either alone or in combination with chitosan oligomers, were assayed in vitro against bacterial and fungal pathogens that affect apple trees (Malus domestica) and grapevine (Vitis vinifera). Remarkable inhibition was observed against Erwinia amylovora, the causal agent of fire blight in apple; Xylophilus ampelinus [syn. Erwinia vitivora], the causal agent of bacterial blight of grapevine; and Diplodia seriata, a virulent pathogen of grapevines that also causes canker, leaf spot and fruit rot of apple. In view of their effectiveness against these three phytopathogens, a potential application of these two medicinal plants in organic farming may be envisaged

Self-consistent treatment of thermal effects in neutron-star post-mergers: observational implications for third-generation gravitational-wave detectors

We assess the impact of accurate, self-consistent modelling of thermal effects in neutron-star merger remnants in the context of third-generation gravitational-wave detectors. This is done through the usage, in Bayesian model selection experiments, of numerical-relativity simulations of binary neutron star (BNS) mergers modelled through: a) nuclear, finite-temperature (or ``tabulated'') equations of state (EoSs), and b) their simplifed piecewise (or ``hybrid'') representation. These cover four different EoSs, namely SLy4, DD2, HShen and LS220. Our analyses make direct use of the Newman-Penrose scalar $\psi_4$ outputted by numerical simulations. Considering a detector network formed by three Cosmic Explorers, we show that differences in the gravitational-wave emission predicted by the two models are detectable with a natural logarithmic Bayes Factor $\log{\cal{B}}\geq 5$ at average distances of $d_L \simeq 50$Mpc, reaching $d_L \simeq 100$Mpc for source inclinations $\iota \leq 0.8$, regardless of the EoS. This impact is most pronounced for the HShen EoS. For low inclinations, only the DD2 EoS prevents the detectability of such modelling differences at $d_L \simeq 150$Mpc. Our results suggest that the usage a self-consistent treatment of thermal effects is crucial for third-generation gravitational wave detectors.Comment: 9 pages, 3 Figure