943 research outputs found
Nurse-patient consultations : a way to navigate in the uncharted waters of a spinal cord injury?
Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly
Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required
Revisiting Ruddick: Feminism, pacifism and non-violence
This article explores feminist contentions over pacifism and non-violence in the contextof the Greenham Common Peace Camp in the 1980s and later developments offeminist Just War Theory. We argue that Sara Ruddick’s work puts feminist pacifism, its radical feminist critics and feminist just war theory equally into question. Although Ruddick does not resolve the contestations within feminism over peace, violence and the questions of war, she offers a productive way of holding the tension between them. In our judgment, her work is helpful not only for developing a feminist political response to the threats and temptations of violent strategies but also for thinking through the question of the relation between violence and politics as such
Transition probabilities for general birth-death processes with applications in ecology, genetics, and evolution
A birth-death process is a continuous-time Markov chain that counts the
number of particles in a system over time. In the general process with
current particles, a new particle is born with instantaneous rate
and a particle dies with instantaneous rate . Currently no robust and
efficient method exists to evaluate the finite-time transition probabilities in
a general birth-death process with arbitrary birth and death rates. In this
paper, we first revisit the theory of continued fractions to obtain expressions
for the Laplace transforms of these transition probabilities and make explicit
an important derivation connecting transition probabilities and continued
fractions. We then develop an efficient algorithm for computing these
probabilities that analyzes the error associated with approximations in the
method. We demonstrate that this error-controlled method agrees with known
solutions and outperforms previous approaches to computing these probabilities.
Finally, we apply our novel method to several important problems in ecology,
evolution, and genetics
Exact and quasiexact solvability of second-order superintegrable quantum systems: I. Euclidean space preliminaries
We show that second-order superintegrable systems in two-dimensional and three-dimensional Euclidean space generate both exactly solvable (ES) and quasiexactly solvable (QES) problems in quantum mechanics via separation of variables, and demonstrate the increased insight into the structure of such problems provided by superintegrability. A principal advantage of our analysis using nondegenerate superintegrable systems is that they are multiseparable. Most past separation of variables treatments of QES problems via partial differential equations have only incorporated separability, not multiseparability. Also, we propose another definition of ES and QES. The quantum mechanical problem is called ES if the solution of Schrödinger equation can be expressed in terms of hypergeometric functions mFn and is QES if the Schrödinger equation admits polynomial solutions with coefficients necessarily satisfying a three-term or higher order of recurrence relations. In three dimensions we give an example of a system that is QES in one set of separable coordinates, but is not ES in any other separable coordinates. This example encompasses Ushveridze's tenth-order polynomial QES problem in one set of separable coordinates and also leads to a fourth-order polynomial QES problem in another separable coordinate set
Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis
We previously reported that Parkinson's disease (PD) kinase LRRK2 phosphorylates a subset of Rab GTPases on a conserved residue in their switch-II domains (Steger et al., 2016) (PMID: 26824392). Here, we systematically analyzed the Rab protein family and found 14 of them (Rab3A/B/C/D, Rab5A/B/C, Rab8A/B, Rab10, Rab12, Rab29, Rab35 and Rab43) to be specifically phosphorylated by LRRK2, with evidence for endogenous phosphorylation for ten of them (Rab3A/B/C/D, Rab8A/B, Rab10, Rab12, Rab35 and Rab43). Affinity enrichment mass spectrometry revealed that the primary ciliogenesis regulator, RILPL1 specifically interacts with the LRRK2-phosphorylated forms of Rab8A and Rab10, whereas RILPL2 binds to phosphorylated Rab8A, Rab10, and Rab12. Induction of primary cilia formation by serum starvation led to a two-fold reduction in ciliogenesis in fibroblasts derived from pathogenic LRRK2-R1441G knock-in mice. These results implicate LRRK2 in primary ciliogenesis and suggest that Rab-mediated protein transport and/or signaling defects at cilia may contribute to LRRK2-dependent pathologies
Three-potential formalism for the three-body scattering problem with attractive Coulomb interactions
A three-body scattering process in the presence of Coulomb interaction can be
decomposed formally into a two-body single channel, a two-body multichannel and
a genuine three-body scattering. The corresponding integral equations are
coupled Lippmann-Schwinger and Faddeev-Merkuriev integral equations. We solve
them by applying the Coulomb-Sturmian separable expansion method. We present
elastic scattering and reaction cross sections of the system both below
and above the threshold. We found excellent agreements with previous
calculations in most cases.Comment: 12 pages, 3 figure
Recovery phase of magnetic storms induced by different interplanetary drivers
Statistical analysis of Dst behaviour during recovery phase of magnetic
storms induced by different types of interplanetary drivers is made on the
basis of OMNI data in period 1976-2000. We study storms induced by ICMEs
(including magnetic clouds (MC) and Ejecta) and both types of compressed
regions: corotating interaction regions (CIR) and Sheaths. The shortest,
moderate and longest durations of recovery phase are observed in ICME-, CIR-,
and Sheath-induced storms, respectively. Recovery phases of strong ( nT) magnetic storms are well approximated by hyperbolic functions
with constant times for all types of drivers
while for moderate ( nT) storms profile can not
be approximated by hyperbolic function with constant because
hyperbolic time increases with increasing time of recovery phase.
Relation between duration and value for storms induced by ICME and
Sheath has 2 parts: and duration correlate at small durations while
they anticorrelate at large durations.Comment: 18 pages, 4 figures, 2 tables, submitted to JGR special issue
"Response of Geospace to High-Speed Streams
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