Transcranial direct current stimulation in psychiatric disorders

The interest in non-invasive brain stimulation techniques is increasing in recent years. Among these techniques, transcranial direct current stimulation (tDCS) has been the subject of great interest among researchers because of its easiness to use, low cost, benign profile of side effects and encouraging results of research in the field. This interest has generated several studies and randomized clinical trials, particularly in psychiatry. In this review, we provide a summary of the development of the technique and its mechanism of action as well as a review of the methodological aspects of randomized clinical trials in psychiatry, including studies in affective disorders, schizophrenia, obsessive compulsive disorder, child psychiatry and substance use disorder. Finally, we provide an overview of tDCS use in cognitive enhancement as well as a discussion regarding its clinical use and regulatory and ethical issues. Although many promising results regarding tDCS efficacy were described, the total number of studies is still low, highlighting the need of further studies aiming to replicate these findings in larger samples as to provide a definite picture regarding tDCS efficacy in psychiatry

Brazilian Cerrado Antioxidant Sources: Cytotoxicity And Phototoxicity In Vitro [fontes De Antioxidantes Do Cerrado Brasileiro: Citotoxicidade E Fototoxicidade In Vitro]

Annona crassiflora (araticum), Eugenia dysenterica (cagaita), and Caryocar brasiliense (pequi) are tropical fruits of the second biggest Brazilian biome: The cerrado. Nowadays, the cerrado faces two different realities: 1) the great possibility of food production since it is considered as the biggest storehouse of the world; and 2) the rich biodiversity that has been newly discovered and known. Previous studies showed that certain cerrado fruits demonstrate high content of total phenols and excellent antioxidant activity in in vitro models. Moreover, using fingerprinting analysis, important bioactive molecules were identified as probably responsible for their antioxidant activity. In this study, the cytotoxicity and phototocixity of ethanolic extracts from cerrado fruits were evaluated using the in vitro Neutral Red Uptake (NRU). Regarding cytotoxicity, the extracts of araticum peel and cagaita seed did not shown any cytotoxic potential up to 300 μg.mL-1. Ethanolic extracts of araticum seed and pequi peel presented low cytotoxic potential and, according to linear regressions, the estimated LD50 were de 831.6 and 2840.7 mg.kg-1, respectively. In the evaluated conditions, only the araticum peel extract presented a phototoxic potential. This is the first attempt to screen the toxicity of cerrado fruits with high antioxidant activity.303814821Almeida, S.P., (1998) Cerrado: Espécies Vegetais Úteis, , Planaltina: Empresa Brasileira de Pesquisa Agropecuária - EMBRAPABorenfreund, E., Puerner, J.A., Toxicity determined in vitro by morphological alterations and neutral red absorption (1985) Toxicology Letters, 24 (2-3), pp. 119-124(2000) Standard Operating Procedure, , CENTRE FOR DOCUMENTATION ANDEVALUATION OFALTERNATIVES TO ANIMAL EXPERIMENTS - ZEBETEUROPEAN CENTRE FOR THE VALIDATION OFALTERNATIVE METHODS - ECVAMTHE EUROPEAN COSMETICS TOILETRYANDPERFUMERYASSOCIATION - COLIPA, In vitro 3T3 NRUphototoxicity test (OJ L 136 2000), Annex V Testing MethodsCorrêa, P., (1984) Dicionário Das Plantas Úteis Do Brasil E Das Exóticas Cultivadas, , Rio de Janeiro: Imprensa NacionalCosta, T.R., Antifungal activity of volatile constituents of Eugenia dysenterica leaf oil (2000) Journal of Ethonopharmacology, 72 (1-2), pp. 111-117Ekwall, B., Screening of toxic compounds in mammalian cell cultures (1983) Annals of the New York Academy of Sciences, 407, pp. 64-77(2002) OPPTS Harmonized Test Guidelines, , ENVIRONMENTAL PROTECTION AGENCY- EPA, Guideline OPPTS 870.1100: Acute oral toxicity, EPA 712-c-02-190Gennari, A., Strategies to replace in vivo acute systemic toxicity testing. The report and recommendations of ECVAM Workshop 50 (2004) Alternative to Laboratory Animals: ATLA, 32 (4), pp. 437-459(2005) Occupational Safety and Health Administrations: A Guide to the GHS of Classification and Labeling of Chemicals, , GLOBALLYHARMONIZEDSYSTEM - GHSGrisham, J.W., Smith, G.J., Predictive and mechanistic evaluation to toxic responses in mammalian cell culture systems (1984) Pharmacological Reviews, 36 (SUPPL. 2), pp. 151S-171SHalle, W., The registry of cytotocixity: Toxicity testing in cell cultures to predict acute toxicity (LD50) and to reduce testing in animals (2003) Alternative to Laboratory Animals: ATLA, 31 (2), pp. 89-198Halliwell, B., Gutteridge, J.M.C., (1999) Free Radicals in Biology and Medicine, , 3rd ed. New York: Oxford University PressHolzhütter, H.G., A general measure of in vitro phototoxicity derived from pairs of dose-response curves and its use for predicting the in vivo phototoxicity of chemicals (1997) Alternative to Laboratory Animals: ATLA, 25 (4), pp. 445-462Jayaprakasha, G.K., Rao, L.J., Phenolic constituents from lichen Parmotrema stuppeum (Nyl.). Hale and their antioxidant activity (2000) Zeitschrift Für Naturforschung. C, Journal of Bioscience, 55 (11-12), pp. 1018-1022Londershausen, M., Molecular mode of action of annonins (1991) Pesticide Science, 33 (4), pp. 427-438Lorenzi, H., (1992) Árvores Brasileiras: Manual De Identificação E Cultivo De Plantas Arbóreas Nativas Do Brasil, 2. , 2. ed. Nova Odessa: PlantarumEvaluation of In Vitro Cytotoxicity Test Methods (2001) Guidance Document on Using in Vitro Data to Estimate in Vivo Starting Doses for Acute Toxicity, , NATIONAL INSTITUTE OFHEALTH- NIH, NIHPublication No. 01-4500(2006) Draft Background Review Document: In Vitro Acute Toxicity Test Methods, , NATIONAL TOXICOLOGYPROGRAM INTERAGENCYCENTER FOR THE EVALUATION OFALTERNATIVE TOXICOLOGICAL METHODS - NICEATM(2003) Test Method Protocol: The BALB/c 3T3 Neutral Red Uptake Cytotoxicity Test: A Test for Basal Cytotoxicity Phase III, , NATIONAL TOXICOLOGYPROGRAM INTERAGENCYCENTER FOR THE EVALUATION OFALTERNATIVE TOXICOLOGICAL METHODS - NICEATM(2001) OECD Guidelines for the Testing of Chemicals: Acute Oral Toxicity - Up-and-down Procedure, , ORGANISATION FOR ECONOMIC CO-OPERATION ANDDEVELOPMENT - OECD, Guideline 425Peters, B., Holzhütter, H.G., In vitro phototoxicity testing: Development and validation of a new concentration response analysis software and biostatistical analyses related to the use of various prediction models (2002) Alternatives to Laboratory Animals: ATLA, 30 (4), pp. 415-432Rodrigues, V.E.G., Carvalho, D.A., (2001) Plantas Medicinais No Domínio Dos Cerrados, , Lavras: Editora da Universidade Federal de LavrasRoesler, R., Antioxidant activity of A. crassiflora: Characterization of major components by electrospray ionization mass spectrometry (2007) Food Chemistry, 104 (3), pp. 1048-1054Roesler, R., Atividade antioxidante de frutas do cerrado (2007) Revista Ciência E Tecnologia De Alimentos, 27 (1), pp. 53-60Roesler, R., Evaluation of the antioxidant properties of the Brazilian cerrado fruit Annona crassiflora (Araticum) (2006) Journal of Food Science, 71 (2), pp. 102-107Septímio, L.R., (1994) A Fitoterapia Baseada Em Ervas Medicinais Do Cerrado, , Brasília, DF: Secretaria de Intercâmbio e Projetos Especiais - SIPEShahidi, F., (1996) Natural Antioxidants: An Overview, , Illinois: AOCS PressSilva, J.A., (1994) Frutas Nativas Dos Cerrados, , Planaltina: Empresa Brasileira de Pesquisa Agropecuária - EMBRAPASilva, S., Tassara, H., (2001) Frutas No Brasil, , 5 ed. São Paulo: Empresa das ArtesSpielmann, H., The international EU/COLIPA in vitro phototoxicity validation study: Results of phase II (blind trial). Part 1: The 3T3 NRUphototoxicity test (1998) Toxicology in Vitro, 12 (3), pp. 305-327Weinberg, M.L., Inhibition of drug-induced contractions of guinea-pig ileum by Annona crassiflora seed extract (1993) The Journal of Pharmacy and Pharmacology, 45 (1), pp. 70-7

The influence of type I diabetes mellitus in periodontal disease induced changes of the gingival epithelium and connective tissue

Periodontal disease constitutes the most frequent chronic diseases in human dentition. Bacterial plaque is the main etiologic agent, although it is the host immune response that causes periodontal tissue destruction. Diabetes is considered an important risk factor, not only for the onset but also for progression of the disease. The aim of this study was to analyze structural changes in the rat gingival epithelium and connective tissue in response to the experimental periodontal disease induced by the ligature technique, under the influence of diabetes. The results showed that experimental periodontal disease is characterized by marked inflammation, affecting both the epithelial and connective tissues, causing degeneration of the dermal papilla, increase in the number of inflammatory cells, destruction of reticulin fibers, and accumulation of dense collagen fibers (fibrosis). These changes were worsened by diabetes, apparently by hampering the inflammatory response and affecting tissue repair of the affected tissues. (c) 2008 Elsevier Ltd. All rights reserved.40428329

Differential diagnosis of Brazilian strains of Citrus tristeza virus by epitope mapping of coat protein using monoclonal antibodies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Citrus tristeza virus (CTV) is one of the most important citrus pathogen, and among Brazilian CTV strains, the genotype Capao Bonito (CB) is the most harmful. Therefore, the coat protein (CP) gene were cloned and expressed as recombinant protein and used to develop four specific monoclonal antibodies (MAbs). Our previously data had showed these MAbs could recognize different strains of CTV and the present goal is to identify the epitopes of the recombinant CP by ELISA screening of overlapping recombinant peptides and to determine the binding specificity of CTV isolates in light of their antigenic domains onto CB strains. Three MAbs, 30.G.02,37.G.11 and 39.07 recognized linear and no identical epitopes, but the fourth MAb, IC.04-12, probably had a conformational epitope, since it could not be identified by ELISA screening. Our previous data revealed MAb IC.04-12 do not recognize CP under denaturing conditions, but can identify weak CTV strains in ELISA involving crop samples. MAb 30.G.02 recognized an extremely conserved sequence and can be classified as "universal" antibody, and, interestingly, the epitope turned out by MAb 39.07 corresponded to severe CTV isolates. So, these MAbs can be applied in a differential screening by ELISA. (C) 2009 Elsevier B.V. All rights reserved.14511825FINEPFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

The influence of type I diabetes mellitus on the expression and activity of gelatinases (matrix metalloproteinases-2 and-9) in induced periodontal disease

Background and Objective: Periodontal disease corresponds to a group of lesions that affect the tooth-supporting tissues present in the dental follicle. Although bacterial plaque is important, the immune response also contributes to the destruction of periodontal tissues. Diabetes mellitus is closely associated with the development, progression and severity of periodontal disease because it not only affects extracellular matrix organization but also the tissue response to inflammation. The objective of the present investigation was to study the influence of diabetes on experimental periodontal disease by evaluating the degradation of extracellular matrix through the analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression and activity, using immunofluorescence, zymography and real-time reverse transcription-polymerase chain reaction. Material and Methods: Wistar rats were divided into normal and diabetic groups and evaluated 0, 15 and 30 d after the induction of periodontal disease by ligature. Results: MMP-2 and -9 were detected in epithelial cells, in the blood vessel endothelium and in connective tissue cells. The same profile of enzymatic expression of MMP-2 and -9 was observed in normal and diabetic animals, with a peak in activity at day 15 of inflammation. However, in diabetic animals, MMP-2 gelatinolytic activity was reduced after the inflammatory stimulus, whereas that of MMP-9 was increased. MMP-2 gene expression decreased with inflammation in both normal groups and groups with diabetes. In contrast, MMP-9 expression increased in normal animals and decreased in diabetic animals after inflammation. Conclusion: The results suggest the involvement of MMP-2 and -9 in the dynamics of periodontal disease and that variation in their expression levels results in differences in tissue organization and wound healing in normal and diabetic animals.431485

The Influence Of Type I Diabetes Mellitus On The Expression And Activity Of Gelatinases (matrix Metalloproteinases-2 And -9) In Induced Periodontal Disease

Background and Objective: Periodontal disease corresponds to a group of lesions that affect the tooth-supporting tissues present in the dental follicle. Although bacterial plaque is important, the immune response also contributes to the destruction of periodontal tissues. Diabetes mellitus is closely associated with the development, progression and severity of periodontal disease because it not only affects extracellular matrix organization but also the tissue response to inflammation. The objective of the present investigation was to study the influence of diabetes on experimental periodontal disease by evaluating the degradation of extracellular matrix through the analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression and activity, using immunofluorescence, zymography and real-time reverse transcription-polymerase chain reaction. Material and Methods: Wistar rats were divided into normal and diabetic groups and evaluated 0, 15 and 30 d after the induction of periodontal disease by ligature. Results: MMP-2 and -9 were detected in epithelial cells, in the blood vessel endothelium and in connective tissue cells. The same profile of enzymatic expression of MMP-2 and -9 was observed in normal and diabetic animals, with a peak in activity at day 15 of inflammation. However, in diabetic animals, MMP-2 gelatinolytic activity was reduced after the inflammatory stimulus, whereas that of MMP-9 was increased. MMP-2 gene expression decreased with inflammation in both normal groups and groups with diabetes. In contrast, MMP-9 expression increased in normal animals and decreased in diabetic animals after inflammation. Conclusion: The results suggest the involvement of MMP-2 and -9 in the dynamics of periodontal disease and that variation in their expression levels results in differences in tissue organization and wound healing in normal and diabetic animals. © 2007 Blackwell Munksgaard.4314854Lindhe, J., (1999) Tratado de Periodontologia Clínica e Implantologia Oral, 3rd Edn., pp. 46-67. , Rio de Janeiro: Rio de JaneiroGarlet, G.P., Avila-Campos, M.J., Milanezi, C.M., Ferreira, B.R., Silva, J.S., Actinobacillus actinomycetemcomitans-induced periodontal disease in mice: Patterns of cytokine, chemokine and chemokine receptor expression and leukocyte migration (2005) Microbes Infect, 7, pp. 738-747Potempa, J., Banbula, A., Travis, J., Role of bacterial proteinases in matrix destruction and modulation of host responses (2000) Periodontol 2000, 24, pp. 153-192Page, R.C., Schroeder, H.E., Pathogenesis of inflammatory periodontal disease. A summary of current work (1976) Lab Invest, 34, pp. 223-228Listgarten, M.A., Pathogenesis of periodontitis (1986) J Clin Periodontol, 13, pp. 418-425Seguier, S., Godeau, G., Brousse, N., Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: A comparative and quantitative study by immunohistochemistry and automated image analysis (2000) J Periodontol, 71, pp. 1079-1085Seguier, S., Gogly, B., Bodineau, A., Godeau, G., Brousse, N., Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue? (2001) J Periodontol, 72, pp. 1398-1406Liu, R.K., Cao, C.F., Meng, H.X., Gao, Y., Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis (2001) J Periodontol, 72, pp. 1545-1553Ejeil, A.-L., Gaultier, F., Igondjo-Tchen, S., Are cytokines linked to collagen breakdown during periodontal disease progression? (2003) J Periodontol, 74, pp. 196-201Györfi, A., Fazekas, Á., Fehér, E., Ender, F., Rosivall, L., Effects of streptozotocin-induced diabetes on neurogenic inflammation of gingivomucosal tissue in rat (1996) J Periodont Res, 31, pp. 249-255Birkedal-Hansen, H., Moore, W.G.I., Bodden, M.K., Matrix metalloproteinases: A review (1993) Crit Rev Oral Biol Med, 4, pp. 197-250Sternlicht, M.D., Werb, Z., How matrix metalloproteinases regulate cell behavior (2001) Annu Rev Cell Dev Biol, 17, pp. 463-516Bok, R.A., Hansell, E.J., Nguyen, T.P., Greeberg, N.M., McKerrow, J.H., Shuman, M.A., Patterns of protease production during prostate cancer progression: Proteomic evidence for cascades in a transgenic model (2003) Prostate Cancer Prostatic Dis, 6, pp. 272-280Björklund, M., Koivunen, E., Gelatinase-mediated migration and invasion of cancer cells (2005) Biochim Biophys Acta, 1755, pp. 37-69Wiseman, B.S., Sternlicht, M.D., Lund, L.R., Site-specific inductive and inhibitory activities of MMP-2 and MMP-3 orchestrate mammary gland branching morphogenesis (2003) J Cell Biol, 162, pp. 1123-1133De Vicente, J.C., Fresno, M.F., Villalain, L., Vega, J.A., Hernandez-Vallejo, G., Expression and clinical significance of matrix metalloproteinase-2 and matrix metalloproteinase-9 in oral squamous cell carcinoma (2005) Oral Oncol, 41, pp. 283-293Kato, K., Hara, A., Kuno, T., Matrix metalloproteinases 2 and 9 in oral squamous cell carcinomas: Manifestation and local localization of their activity (2005) J Cancer Res Clin Oncol, 131, pp. 340-346Patel, B.P., Shah, P.M., Rawal., U.M., Activation of MMP-2 and MMP-9 in patients with oral squamous cell carcinoma (2005) J Surg Oncol, 90, pp. 81-88Souza, A.P., Trevilatto, P.C., Scarel-Caminaga, R.M., Brito Jr., R.B., Barros, S.P., Line, S.R.P., Analysis of the MMP-9 (C-1562T) and TIMP-2 (G-418C) gene promoter polymorphisms in patients with chronic periodontitis (2005) J Clin Periodontol, 32, pp. 207-211Xue, M., Thompson, P.J., Clifton-Bligh, R., Fulcher, G., Gallery, E.D.M., Jackson, C., Leukocyte matrix metalloproteinase-9 is elevated and contributes to lymphocyte activation in type i diabetes (2005) Int J Biochem Cell Biol, 37, pp. 2406-2416Bender, I.B., Bender, A.B., Diabetes mellitus and the dental pulp (2003) J Endod, 29, pp. 383-389Robbins, S.L., Doenças Sistêmicas, T.N., Diabetes Mellitus (1989) Patologia Estrutural e Funcional, pp. 238-250. , In: Cotran, R.S., Kumar, V.Y., Collins, T. 3rd ednConget, I., Diagnosis, classification and pathogenesis of diabetes mellitus (2002) Rev Esp Cardiol, 55, pp. 528-538Wada, L.Y., (2002) Contagem de Carboidratos: Mais Fácil Que Contar Até 3, 1st Edn., pp. 3-25. , São Paulo: São PauloMaxwell, P.R., Timms, P.M., Chandran, S., Gordon, D., Peripheral blood level alterations of TIMP-1, MMP-2 and MMP-9 in patients with type i diabetes (2001) Diabet Med, 18, pp. 777-780Kadoglou, N.P., Daskalopoulou, S.S., Perrea, D., Liapis, C.D., Matrix metalloproteinases and diabetic vascular complications (2005) Angiology, 56, pp. 173-189Diabetes and periodontal disease (2000) J Periodontol, 71, pp. 664-678. , Position PaperPersson, R.E., Hollender, L.G., MacEntee, M.I., Wyatt, C.C.L., Kiyak, H.A., Persson, G.R., Assessment of periodontal conditions and systemic disease in older subjects - Focus on diabetes mellitus (2003) J Clin Periodontol, 30, pp. 207-213Silva, J.A.F., (2006), Análise da influência do diabetes tipo I induzido na progressão da doença periodontal experimental em ratos. Campinas, SP: State University of Campinas, DissertationCarson, K.A., (1979) Cytochemical and Cytopathological Studies of Nerve, Vascular and Salivary Gland Tissues in An Hereditary Mouse Model for Diabetes Mellitus, , Chapel Hill, NC: University of North Carolina, DissertationJohnson, I.H., Effects of local irritation and dextran sulphate administration on the periodontium of the rat (1975) J Periodont Res, 10, pp. 332-345Bradford, M.M., A rapid and sensitive method of quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254Ponte, E., Tabaj, D., Maglione, M., Melato, M., Diabetes mellitus and oral disease (2001) Acta Diabetol, 38, pp. 57-62Southerland, J.H., Taylor, G.W., Moss, K., Beck, J.D., Offenbacher, S., Commonality in chronic inflammation diseases: Periodontitis, diabetes, and coronary artery disease (2006) Periodontol 2000, 40, pp. 130-143Taylor, G.W., Bidirectional interrelationships between diabetes and periodontal diseases: An epidemiologic perspective (2001) Ann Periodontol, 6, pp. 99-112Holzhausen, M., Garcia, D.F., Pepato, M.T., Marcantonio Jr., E., The influence of short-term diabetes mellitus and insulin therapy on alveolar bone loss in rats (2004) J Periodont Res, 39, pp. 188-193Makela, M., Sato, T., Uitto, V., Larjava, H., Matrix metalloproteinases (MMP-2 and MMP-9) of the oral cavity: Cellular origin and relationship to periodontal status (1994) J Dent Res, 73, pp. 1397-1406Aiba, T., Akeno, N., Kawane, T., Okamoto, H., Horiuchi, N., Matrix metalloproteinases-1 and -8 and TIMP-1 mRNA levels in normal and diseased human gingivae (1996) Eur J Oral Sci, 104, pp. 562-569Garlet, G.P., Cardoso, C.R., Silva, T.A., Cytokine pattern determines the progression of experimental periodontal disease induced by Actinobacillus actinomycetemcomitans through the modulation of MMPs, RANKL, and their physiological inhibitors (2006) Oral Microbiol Immunol, 21, pp. 12-20Ingman, T., Tervahartala, T., Ding, Y., Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients (1996) J Clin Periodontol, 23, pp. 1127-1132Zhou, J., Windsor, J., Porphyromonas gingivalis affects host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases (2006) J Periodont Res, 41, pp. 47-54Saito, T., Shimazaki, Y., Kiyohaba, Y., The severity of periodontal disease is associated with the development of glucose intolerance in non-diabetics: The Hisama study (2004) J Dent Res, 83, pp. 485-490Bartold, P.M., Narayanan, A.S., Molecular and cell biology of healthy and diseased periodontal tissues (2006) Periodontol 2000, 40, pp. 29-4

A experiência do núcleo de estudos de meios de solução de conflictos (NEMESC)

This paper presents the 5 years' experience of the alternative dispute resolution group (NEMESC) established at the University of Sao Paulo Law School. The nemesc begun as a studying and reading group interested to debate and to research about alternative dispute resolution issues, and now a course emerged from this studying group at the law school. This paper considers the activities and practices developed by the group, the methodological choices and students' involvement during the 5 years of the nemesc experience to show some positive results. The paper goal is contributing to the debates about alternative dispute resolution and to the exchange of views and experiences about adr at the law school environment.Fil: Salles, Carlos Alberto de. Universidad de San Pablo. Facultad de Derecho. Cátedra Derecho Procesal. San Pablo, BrasilFil: Gabbay, Daniela M. Universidad de San Pablo. Facultad de Derecho. San Pablo, BrasilFil: Silva, Erica B. Universidad de San Pablo. Facultad de Derecho. San Pablo, BrasilFil: Tartuce, Fernanda. Universidad de San Pablo. Facultad de Derecho. San Pablo, BrasilFil: Guerrero, Luis Fernando. Universidad de San Pablo. Facultad de Derecho. San Pablo, BrasilFil: Garcia Lopes Lorencini, Marco Antônio. Universidad de San Pablo. Facultad de Derecho. Cátedra Derecho Civil. San Pablo, BrasilFil: Garcia Lopes Lorencini, Marco Antônio. Universidad de San Pablo. Facultad de Derecho. Cátedra Derecho Procesal Civil. San Pablo, BrasilFe de erratas publicada en Academia, año 10, nro. 20, 2012. -- "Por razones relacionadas con los plazos de las publicaciones ajenas a los editores, en Academia. Revista sobre enseñanza del Derecho, año 9, nro. 18, 2011, pp. 33-57, fue publicado el trabajo de Carlos Alberto de Salles, Daniela M. Gabbay, Erica B. Silva, Fernanda Tartuce, Luis Fernando Guerrero y Marco Antônio G. L. Lorencini, "A experiência do núcleo de estudos de meios de solução de conflitos (NEMESC)" sin referencia a su publicación en: Salles, Carlos Alberto de et al., "A experiencia do núcleo de estudos de meios de solução de conflitos (NEMESC)", en Rev. Direito GV, junio de 2010, vol. 6, nro. 1, pp. 67-94, ISSN 1808-2432, que puede ser consultada en https://www.scielo.br/cgi-bin/wxis.exe/iah/ ". -- El artículo presenta la experiencia desarrollada por el núcleo de estudios de medios de solución de conflictos (NEMESC) en el ámbito de la Facultad de Derecho de la Universidad de San Pablo. Las actividades, que cumplieron cinco años en 2009, tuvieron inicio informalmente a través de un grupo de estudio con alumnos de grado y posgrado y hoy se consagraron como disciplinas de extensión de la Facultad de Derecho. Este artículo considera las actividades y prácticas desarrolladas por el grupo, las elecciones metodológicas y el compromiso de los alumnos durante estos cinco años de experiencia del NEMESC, a fin de contribuir en el debate sobre el tema y sobre el cambio de experiencias en medios de solución de conflictos en el ámbito de la Facultad de Derecho.\