Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells

AbstractHere we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies

In vitro and in vivo anti-Trypanosoma cruzi activity of new arylamine Mannich base-type derivatives

Chagas disease is a neglected tropical disease with 6-7 million people infected worldwide and there is no effective treatment. Therefore, there is an urgent need to continue researching in order to discover novel therapeutic alternatives. We present a series of arylaminoketone derivatives as means of identifying new drugs to treat Chagas disease in the acute phase with greater activity, less toxicity and with a larger spectrum of action than that corresponding to the reference drug benznidazole. Indexes of high selectivity found in vitro formed the basis for later in vivo assays in BALB/c mice. Murine model results show that compounds 3, 4, 7 and 10 induced a remarkable decrease in parasitemia levels in acute phase and the parasitemia reactivation following immunosuppression, and curative rates were higher than with benznidazole. These high anti-parasitic activities encourage us to propose these compounds as promising molecules for developing an easy to synthesize anti-Chagas agent

On the binary nature of 1RXS J162848.1-415241

We present spectroscopy of the optical counterpart to 1RXS J162848.1-41524, also known as the microquasar candidate MCQC J162847-4152. All the data indicate that this X-ray source is not a microquasar, and that it is a single-lined chromospherically active binary system with a likely orbital period of 4.9 days. Our analysis supports a K3IV spectral classification for the star, which is dominant at optical wavelengths. The unseen binary component is most likely a late-type (K7-M) dwarf or a white dwarf. Using the high resolution spectra we have measured the K3 star's rotational broadening to be vsini = 43 +/- 3 km/s and determined a lower limit to the binary mass ratio of q(=M2/M1)>2.0. The high rotational broadening together with the strong CaII H & K / Halpha emission and high-amplitude photometric variations indicate that the evolved star is very chromospherically active and responsible for the X-ray/radio emission.Comment: 15 pages, 5 figures, accepted for publication in Ap

Yam improvement for income and food security in West Africa: Effectiveness of a multi-disciplinary and multi-institutional team-work

The overall goal of the five year project “Yam Improvement for Income and Food Security in West Africa” (YIIFSWA) funded by the Bill and Melinda Gates Foundation and led by the International Institute of Tropical Agriculture, Ibadan, Nigeria was to: (a) increase yam productivity by 40% for 2,00,000 smallholder yam farmers in Ghana and Nigeria and (b) deliver key global good research products that will contribute to the 10-year overall vision to sustainably double incomes from yams for 3 million smallholder yam farming families and contribute to ensuring food security for producers and consumers. Implemented by scientists of twenty partner specialized organizations, comprising research institutes, Universities, governmental and non-governmental organizations, YIIFSWA has impacted yam value chain stakeholders through research and development interventions. The significant contributions made in the project during the past 18 months’ period are discussed in this paper. These include: a baseline survey conducted in key yam growing areas in Ghana and Nigeria, training of yam producers on adapted yam minisett technique and production of seed yam, undertaking in depth value chain assessments, developing the capacity of Farmers Organizations (FOs) by linking them to service providers (SPs), participatory evaluation of new yam genotypes, successful development of yam virus diseases diagnostics and development of novel techniques for high ratio yam propagation such as aeroponics and bioreactors

Changes in plasma fatty acid composition are associated with improvements in obesity and related metabolic disorders: A therapeutic approach to overweight adolescents

Background & aims: In recent years, obesity has reached alarming levels among children and adolescents. The study of plasma fatty acid (FA) composition, as a reflection of diet, and its associations with other parameters, that are closely linked to obesity and the cardiometabolic profile, may be useful for setting nutritional goals for obesity treatment and prevention. This study explored the relationship between plasma FA levels and body fat and cardiometabolic risk markers, in overweight adolescents. Methods: A multidisciplinary weight loss program was followed by 127 overweight and obese adolescents aged 12-17 years old. Plasma FA composition, anthropometric indicators of adiposity and biochemical parameters were analyzed at baseline, two months (the end of the intensive intervention phase) and six months (the end of the extensive phase). Results: While saturated fatty acid (SFA) and n-6 polyunsaturated fatty acid (PUFA) levels decreased significantly during the intervention, monounsaturated fatty acid (MUFA) and n-3 PUFA showed the opposite trend. The decrease in SFA C14:0 was associated with a reduction in total and LDL cholesterol, apolipoprotein B and insulin. The increase in MUFAs, especially C18:1n-9, was related to a reduction in weight, fat mass, fat mass index and glucose. Regarding PUFAs, changes in the n-3 series were not associated with any of the parameters studied, whereas the reduction in n-6 PUFA5 was directly related to weight, fat mass, total and HDL cholesterol, apolipoprotein Al, glucose and insulin, and inversely associated with diastolic blood pressure. The adolescents with greater weight loss presented significant changes in MUFAs, n-6 PUFA5 and C14:0. Conclusions: Modifications in plasma FA composition were associated with adiposity reduction and cardiometabolic profile improvement in an anti-obesity program aimed at adolescents. The changes observed in FA composition were related to the success of the treatment, since the individuals most affected by these variations were those who presented the greatest weight loss

Hidrogenionic potential (pH) of the attractant, trap density and control threshold for Ceratitis capitata (Diptera: tephritidae) on Hamlin oranges in São Paulo central region, Brazil

This study evaluated the effect of initial pH values of 4.5, 6.5 and 8.5 of the attractant (protein bait) Milhocina® and borax (sodium borate) in the feld, on the capture of fruit flies in McPhail traps, using 1, 2, 4 and 8 traps per hectare, in order to estimate control thresholds in a Hamlin orange grove in the central region of the state of São Paulo. The most abundant fruit fly species was Ceratitis capitata, comprising almost 99% of the fruit flies captured, of which 80% were females. The largest captures of C. capitata were found in traps baited with Milhocina® and borax at pH 8.5. Captures per trap for the four densities were similar, indicating that the population can be estimated with one trap per hectare in areas with high populations. It was found positive relationships between captures of C. capitata and the number of Hamlin oranges damaged, 2 and 3 weeks after capture. It was obtained equations that correlate captures and damage levels which can be used to estimate control thresholds. The average loss caused in Hamlin orange fruits by C. capitata was 2.5 tons per hectare or 7.5% of production.Esta pesquisa teve como objetivos: avaliar o efeito do pH inicial, 4.5; 6.5 e 8.5, do atrativo proteico Milhocina® e bórax (tetraborato de sódio) na captura de moscas-das-frutas em armadilhas McPhail; estudar densidades de armadilhas, 1; 2; 4 e 8 por hectare, para estimar níveis de controle em laranja cv. Hamlin, na região central de São Paulo. A espécie predominante, com 99% das moscas-das-frutas capturadas, foi Ceratitis capitata, sendo 80% de fêmeas. As maiores capturas de C. capitata ocorreram nas armadilhas com Milhocina® e bórax em pH 8.5. As capturas, nas 4 densidades, foram semelhantes, indicando que a população pode ser estimada com uma armadilha por hectare em áreas de altas populações. Houve relações positivas entre capturas de C. capitata e o número de frutos danificados, 2 e 3 semanas após a captura. Assim, foram obtidas equações que relacionam a captura e o dano, possibilitando estimar níveis de controle desse inseto. As perdas médias causadas por C. capitata em laranja cv. Hamlin chegaram a 2,5 toneladas de frutos por hectare ou 7,5% da produção.info:eu-repo/semantics/publishedVersio

Dark Matter and Higgs Sector

The inert doublet model is an extension of the Standard Model of Elementary Particles that is defined by the only addition of a second Higgs doublet without couplings to quarks or leptons. This minimal framework has been studied for many reasons. In particular, it has been suggested that the new degrees of freedom contained in this doublet can account for the Dark Matter of the Universe.Comment: 6 pages, 3 figures,To appear in the Proceedings of the sixth International Workshop on the Dark Side of the Universe (DSU2010) Leon, Guanajuato, Mexico 1-6 June 201