Emergency evacuation system for all

Projecte del programa Erasmus EPSTreball desenvolupat dins el marc del programa 'European Project Semester'.A good emergency evacuation plan for buildings is a key factor for saving lives in the case of an emergency. Therefore, the purpose of the project “Emergency Evacuation System for All” is ensuring the appliance of evacuation methodology, being as efficient and effective as possible, in order to maintain a maximum amount of safety for able and disabled people. Major outcome of the project is a complete emergency evacuation system, taking all people into account for buildings with a special focus on public buildings such as university buildings. The project contains a general evacuation plan for all people, an applied evacuation plan for the main building at the Escola Politècnica Superior d'Enginyeria de Vilanova i la Geltrú, a new technical evacuation solution for buildings, a report on existing technical solutions, a training plan for emergency helpers and a business plan for La Càtedra d’Accessibilitat, a non porfit organization with the main goal to improve society standarts concering persons with disabilities

Control del presupuesto y su incidencia en la rentabilidad del Consorcio Educativo Mentes Brillantes SAC, Trujillo 2019

El trabajo de investigación que se presenta tiene como objetivo principal determinar si un adecuado control del presupuesto mejora la rentabilidad del Consorcio Educativo Mentes Brillantes SAC, en el periodo 2019. Ubicada en la ciudad de Trujillo. Para su desarrollo hemos empleado la herramienta del control del presupuesto con la técnica de entrevista, cuestionarios, y análisis documental, para identificar los ingresos, costos y gastos de las operaciones realizadas por el Consorcio Educativo Mentes Brillantes SAC y en el cual se ha podido evidenciar que tienen bastantes deficiencias, uno de ellos es el no llevar un control de presupuesto por lo que ha generado una considerable pérdida en el ejercicio 2019, a tal punto que los ingresos no cubren los costos y gastos viéndose en la obligación de solicitar préstamo al gerente para cubrir el déficit. Para finalizar podemos decir que los indicadores determinaron que los resultados obtenidos del Consorcio Educativo Mentes Brillantes SAC, no son rentables porque no se cumple con el compromiso de ejecución, no se tiene un control, y ningún buen manejo del presupuesto, si se continúa así el consorcio corre el riesgo de permanencia en el mercado

Identification and quantification of fumonisin-producing Fusarium species in grain and soil samples from Egypt and the Philippines

Fumonisins are considered among the important mycotoxins associated with human esophageal cancer and livestock diseases. These mycotoxins are mainly produced by Fusarium verticillioides in tropical and subtropical regions such as the Philippines and Egypt and humid temperate regions of the world. The classical taxonomy of fumonisin-producing fungi is challenging, and species-specific PCR reactions are commonly used to clearly identify species within these complexes. The aim of this study was to isolate, identify and quantify fumonisin-producing species in maize, wheat and soil samples from Egypt and the Philippines, and to test Eppendorf-Agar as a long term preservation method. We isolated 44 single spore isolates (39 from Egypt and five from the Philippines) from the collected samples (25 isolates from maize, five from wheat and 14 from soil). In addition, we quantified the content of fumonisin-producing fungi DNA from 15 maize samples and six wheat samples from Egypt, and from six maize samples from the Philippines. morphological and microscopic identification indicated that 21 isolates from Egypt and five from the Philippines were F. verticillioides, one isolate was F. proliferatum and two isolates were F. nygamai. Molecular identification indicated that all these isolates belonged to F. verticillioides. Most were from maize, four were from soil and only one was from wheat. Other Fusarium species isolated included F. oxysporum and F. solani. No F. graminearum isolates were found. The quantitative PCR (qPCR) results obtained using the Taqfum-2f, Vpgen-3R primer pair and the FUMp probe for quantification of fumonisin-producing Fusarium species showed that fumonisin-producing Fusarium isolates were present in four maize samples from the Philippines and eight maize samples from Egypt. The Fusarium DNA levels from fumonisin-producing isolates were in the range of 13 × 10-3 to 61 × 10-1 ng ng-1 total DNA in positive samples, except in one maize sample from the Philippines with high concentration of >0.5 ng ng-1 total DNA. This indicates that >50 % of all DNA was Fusarium DNA. No fumonisin-producing Fusarium DNA was detected in the wheat samples and in the remaining maize samples. These results showed that PCR-techniques based on qPCR can be used to identify fumonisin-producing Fusarium species and quantify risks of mycotoxin contaminated grains

Geographic Distribution of Avirulence Genes of the Rice Blast Fungus Magnaporthe oryzae in the Philippines

A total of 131 contemporary and 33 reference isolates representing a number of multi-locusgenotypes of Magnaporthe oryzae were subjected to a PCR test to detect the presence/absence ofavirulence (Avr) genes. Results revealed that the more frequently occurring genes were Avr-Pik(81.50%), Avr-Pita (64.16%) and Avr-Pii (47.98%), whereas the less frequently occurring genes wereAvr-Pizt (19.08%) and Avr-Pia (5.20%). It was also laid out that the presence of Avr genes in M. oryzaeis strongly associated with agroecosystems where the complementary resistant (R) genes exist.No significant association, however, was noted on the functional Avr genes and the major geographiclocations. Furthermore, it was identified that the upland varieties locally known as “Milagrosa” and“Waray” contained all the R genes complementary to the Avr genes tested.<br /

Manejo sanitario de frutales : mosca de la fruta

Las Moscas de los Frutos pertenecen a la clase insceta, orden disptera y familia tephritidae, dentro de la cual se han descripto en el mundo más de 4000 especies. Los géneros más importantes por su carácter de plagas cuarentenarias son: Ceratitis, Anastrepha, Rhagoletis, Bractocera y Toxothripana. Para Argentina han sido citadas Anastrepha fraterculus para la zona norte y Ceratitis capitata en casi la totalidad del territorioEEA San JuanFil: Gómez, María Pía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Gómez, María Pía. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jofré Barud, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: López, María Liza. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: López, María Liza. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sosa, María Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina

Identification and quantification of fumonisin-producing Fusarium species in grain and soil samples from Egypt and the Philippines

Fumonisins are considered among the important mycotoxins associated with human esophageal cancer and livestock diseases. These mycotoxins are mainly produced by Fusarium verticillioides in tropical and subtropical regions such as the Philippines and Egypt and humid temperate regions of the world. The classical taxonomy of fumonisin-producing fungi is challenging, and species-specific PCR reactions are commonly used to clearly identify species within these complexes. The aim of this study was to isolate, identify and quantify fumonisin-producing species in maize, wheat and soil samples from Egypt and the Philippines, and to test Eppendorf-Agar as a long term preservation method. We isolated 44 single spore isolates (39 from Egypt and five from the Philippines) from the collected samples (25 isolates from maize, five from wheat and 14 from soil). In addition, we quantified the content of fumonisin-producing fungi DNA from 15 maize samples and six wheat samples from Egypt, and from six maize samples from the Philippines. morphological and microscopic identification indicated that 21 isolates from Egypt and five from the Philippines were F. verticillioides, one isolate was F. proliferatum and two isolates were F nygamai. Molecular identification indicated that all these isolates belonged to F. verticillioides. Most were from maize, four were from soil and only one was from wheat. Other Fusarium species isolated included F. oxysporum and F. solani. No F. graminearum isolates were found. The quantitative PCR (qPCR) results obtained using the Taqfum-2f, Vpgen-3R primer pair and the FUMp probe for quantification of fumonisin-producing Fusarium species showed that fumonisin-producing Fusarium isolates were present in four maize samples from the Philippines and eight maize samples from Egypt. The Fusarium DNA levels from fumonisin-producing isolates were in the range of 13 x 10(-3) to 61 x 10(-1) ng ng(-1) total DNA in positive samples, except in one maize sample from the Philippines with high concentration of >0.5 ng ng(-1) total DNA. This indicates that >50 % of all DNA was Fusarium DNA. No fumonisin-producing Fusarium DNA was detected in the wheat samples and in the remaining maize samples. These results showed that PCR-techniques based on qPCR can be used to identify fumonisin-producing Fusarium species and quantify risks of mycotoxin contaminated grains

Essential Oil of Azorella cryptantha Collected in Two Different Locations from San Juan Province, Argentina: Chemical Variability and Anti-Insect and Antimicrobial Activities

Essential oils of two populations of Azorella cryptantha (Clos) Reiche, a native species from San Juan province, were obtained by hydrodistillation in a Clevenger-type apparatus and characterized by GC/FID and GC/MS. The compounds identified were 92.3% and 88.7% for A. cryptantha from Bauchaceta and A. cryptantha from Agua Negra respectively. The essential oil composition was similar for both populations with differences in the main components, and in the identity of minor components. The main components of A. cryptantha-BAU essential oil were sabinene (4), betha pinene (5) and delta-cadinol (38) while α-thujene (1), alpha pinene (2), bethe pinene (5) and, delta cadinene (29) were the main monoterpene hydrocarbons in A. cryptantha-AN essential oil wih the addition of 3.0% of oxygenated monoterpenes. Repellent activity on T. infestans nymphs was 100% and 92% for A. cryptantha-AN and A. cryptantha-BAU essential oils respectively. Regarding the toxic effects on C. capitata, the oils were very active with LD50 values of less than 11 µg/fly. Microsporum gypseum, Trichophyton rubrum and T. mentagrophytes and bacteria LM1-E. coli, LM2-E. coli and PI-Yersinia enterocolitica were more sensitive toward the species collected in Agua Negra (MIC equal 125μg/ml). This is the first report on the anti-insect and antimicrobial properties of A. cryptantha essential oil and its composition.Fil: Lopez, Sandra. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Aragon, Liliana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Ariza Espinar, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Tapia, Aníbal Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario; ArgentinaFil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Feresin, Gabriela Egly. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: López, María Liza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Efficacy of lisdexamfetamine dimesylate throughout the day in children and adolescents with attention-deficit/hyperactivity disorder:results from a randomized, controlled trial

Lisdexamfetamine dimesylate (LDX) is a long-acting, prodrug stimulant therapy for patients with attention-deficit/hyperactivity disorder (ADHD). This randomized placebo-controlled trial of an optimized daily dose of LDX (30, 50 or 70 mg) was conducted in children and adolescents (aged 6–17 years) with ADHD. To evaluate the efficacy of LDX throughout the day, symptoms and behaviors of ADHD were evaluated using an abbreviated version of the Conners’ Parent Rating Scale-Revised (CPRS-R) at 1000, 1400 and 1800 hours following early morning dosing (0700 hours). Osmotic-release oral system methylphenidate (OROS-MPH) was included as a reference treatment, but the study was not designed to support a statistical comparison between LDX and OROS-MPH. The full analysis set comprised 317 patients (LDX, n = 104; placebo, n = 106; OROS-MPH, n = 107). At baseline, CPRS-R total scores were similar across treatment groups. At endpoint, differences (active treatment − placebo) in least squares (LS) mean change from baseline CPRS-R total scores were statistically significant (P < 0.001) throughout the day for LDX (effect sizes: 1000 hours, 1.42; 1400 hours, 1.41; 1800 hours, 1.30) and OROS-MPH (effect sizes: 1000 hours, 1.04; 1400 hours, 0.98; 1800 hours, 0.92). Differences in LS mean change from baseline to endpoint were statistically significant (P < 0.001) for both active treatments in all four subscales of the CPRS-R (ADHD index, oppositional, hyperactivity and cognitive). In conclusion, improvements relative to placebo in ADHD-related symptoms and behaviors in children and adolescents receiving a single morning dose of LDX or OROS-MPH were maintained throughout the day and were ongoing at the last measurement in the evening (1800 hours)

Sequence specificity of single-stranded DNA-binding proteins: a novel DNA microarray approach

We have developed a novel DNA microarray-based approach for identification of the sequence-specificity of single-stranded nucleic-acid-binding proteins (SNABPs). For verification, we have shown that the major cold shock protein (CspB) from Bacillus subtilis binds with high affinity to pyrimidine-rich sequences, with a binding preference for the consensus sequence, 5′-GTCTTTG/T-3′. The sequence was modelled onto the known structure of CspB and a cytosine-binding pocket was identified, which explains the strong preference for a cytosine base at position 3. This microarray method offers a rapid high-throughput approach for determining the specificity and strength of ss DNA–protein interactions. Further screening of this newly emerging family of transcription factors will help provide an insight into their cellular function

Molecular Quantification and Genetic Diversity of Toxigenic Fusarium Species in Northern Europe as Compared to Those in Southern Europe

Fusarium species produce important mycotoxins, such as deoxynivalenol (DON), nivalenol (NIV) and T-2/HT-2-toxins in cereals. The highest DON and T-2/HT-2 toxin levels in northern Europe have been found in oats. About 12%&ndash;24% of Finnish oat samples in 2012 contained &gt;1.75 mg&middot;kg&minus;1 of DON, which belongs to type B trichothecenes. Fusarium graminearum is the most important DON producer in northern Europe and Asia and it has been displacing the closely related F. culmorum in northern Europe. The 3ADON chemotype of F. graminearum is dominant in most northern areas, while the 15ADON chemotype of F. graminearum is predominating in Central and southern Europe. We suggest that the northern population of F. graminearum may be more specialized to oats than the southern population. Only low levels of F. culmorum DNA were found in a few oat samples and no correlation was found between F. culmorum DNA and DON levels. DNA levels of F. graminearum were in all cases in agreement with DON levels in 2011 and 2012, when DON was measured by gas chromatography-mass spectrometry (GC-MS). When the RIDA&reg; QUICK SCAN kit results (DON) were compared to DNA levels of F. graminearum, the variation was much higher. The homogenization of the oats flour by grinding oats with 1 mm sieve seems to be connected to this variation. There was a significant correlation between the combined T-2 and HT-2 and the combined DNA levels of F. langsethiae and F. sporotrichioides in Finland in 2010&ndash;2012. Keywords: Fusarium; mycotoxins; diversity; Europe; qPCR <! [endif] --