Towards an incremental deployment of ERN protocols: a proposal for an E2E-ERN hybrid protocol

We propose an architecture based on a hybrid E2E-ERN approach to allow incremental deployment of ERN (Explicit Rate Notification) protocols in heterogeneous networks. The proposed IP-ERN architecture combines E2E (End-to-End)and ERN protocols and uses the minimum between both congestion windows to perform. Without introducing complex operation, the resulting E2E-ERN protocol provides inter and intra protocol fairness and benefits from all ERN protocol advantages when possible. We detail the principle of this novel IP-ERN architecture and show that this architecture is highly adaptive to the network dynamic and is compliant with IPv4, IPv6 as well as IP-in-IP tunneling solutions

SatERN: a PEP-less solution for satellite communications

In networks with very large delay like satellite IPbased networks, standard TCP is unable to correctly grab the available resources. To overcome this problem, Performance Enhancing Proxies (PEPs), which break the end-to-end connection and simulate a receiver close enough to the sender, can be placed before the links with large delay. Although splitting PEPs does not modify the transport protocol at the end nodes, they prevent the use of security protocols such as IPsec. In this paper, we propose solutions to replace the use of PEPs named SatERN. This proposal, based on Explicit Rate Notification (ERN) protocols over IP, does not split connections and is compliant with IP-in-IP tunneling solutions. Finally, we show that the SatERN solution achieves high satellite link utilization and fairness of the satellite traffic

Agile low phase noise radio-frequency sine wave generator applied to experiments on ultracold atoms

We report on the frequency performance of a low cost (~500$) radio-frequency sine wave generator, using direct digital synthesis (DDS) and a field-programmable gate array (FPGA). The output frequency of the device may be changed dynamically to any arbitrary value ranging from DC to 10 MHz without any phase slip. Sampling effects are substantially reduced by a high sample rate, up to 1 MHz, and by a large memory length, more than 2.10^5 samples. By using a low noise external oscillator to clock the DDS, we demonstrate a phase noise as low as that of the master clock, that is at the level of -113 dB.rad^2/Hz at 1 Hz from the carrier for an output frequency of 3.75 MHz. The device is successfully used to confine an ultracold atomic cloud of rubidium 87 in a RF-based trap, and there is no extra heating from the RF source.Comment: 10 pages, 6 figure

Conservation of alternative polyadenylation patterns in mammalian genes

BACKGROUND: Alternative polyadenylation is a widespread mechanism contributing to transcript diversity in eukaryotes. Over half of mammalian genes are alternatively polyadenylated. Our understanding of poly(A) site evolution is limited by the lack of a reliable identification of conserved, equivalent poly(A) sites among species. We introduce here a working definition of conserved poly(A) sites as sites that are both (i) properly aligned in human and mouse orthologous 3' untranslated regions (UTRs) and (ii) supported by EST or cDNA data in both species. RESULTS: We identified about 4800 such conserved poly(A) sites covering one third of the orthologous gene set studied. Characteristics of conserved poly(A) sites such as processing efficiency and tissue-specificity were analyzed. Conserved sites show a higher processing efficiency but no difference in tissular distribution when compared to non-conserved sites. In general, alternative poly(A) sites are species-specific and involve minor, non-conserved sites that are unlikely to play essential roles. However, there are about 500 genes with conserved tandem poly(A) sites. A significant fraction of these conserved tandems display a conserved arrangement of major/minor sites in their 3' UTR, suggesting that these alternative 3' ends may be under selection. CONCLUSION: This analysis allows us to identify potential functional alternative poly(A) sites and provides clues on the selective mechanisms at play in the appearance of multiple poly(A) sites and their maintenance in the 3' UTRs of genes

Differential Repression of Alternative Transcripts: A Screen for miRNA Targets

Alternative polyadenylation sites produce transcript isoforms with 3′ untranslated regions (UTRs) of different lengths. If a microRNA (miRNA) target is present in the UTR, then only those target-containing isoforms should be sensitive to control by a cognate miRNA. We carried out a systematic examination of 3′ UTRs containing multiple poly(A) sites and putative miRNA targets. Based on expressed sequence tag (EST) counts and EST library information, we observed that levels of isoforms containing targets for miR-1 or miR-124, two miRNAs causing downregulation of transcript levels, were reduced in tissues expressing the corresponding miRNA. This analysis was repeated for all conserved 7-mers in 3′ UTRs, resulting in a selection of 312 motifs. We show that this set is significantly enriched in known miRNA targets and mRNA-destabilizing elements, which validates our initial hypothesis. We scanned the human genome for possible cognate miRNAs and identified phylogenetically conserved precursors matching our motifs. This analysis can help identify target-miRNA couples that went undetected in previous screens, but it may also reveal targets for other types of regulatory factors

Mytillocactus (cactaecea) : botanical, agronomic, physichemical and chemical characteristics of fruits

Au Mexique, la production de fruits de Myrtillocactus est importante, pourtant ceux-ci sont souvent sous-utilisés. Bien qu'ils soient essentiellement exploités pour leur potentiel de colorants alimentaires, les fruits mériteraient davantage d'attention quant à leurs autres propriétés pour l'alimentation, mais les informations sur leur composition physico-chimique sont rares. Afin de disposer d'éléments de base pour le développement de la culture de Myrtillocactus, nous avons étudié les informations accessibles à ce jour. Description botanique. Quatre espèces de Myrtillocactus ont été identifiées et rapportées dans la littérature. Elles diffèrent par la forme, la couleur, et d'autres caractéristiques phénotypiques. Au Mexique, l'espèce prédominante est M. geometrizans, mais M. schenckii se développe également abondamment dans toutes les terres arides et semi-arides du pays. Les analyses cytologiques effectuées sur M. geometrizans ont montré que la plante était diploïde (2n = 22). Aspects agronomiques. Le genre Myrtillocactus appartient à la famille des cactacées. En raison de son métabolisme crassulacéen acide, la plante peut se développer dans les montagnes arides et semi-arides du Mexique. Les espèces de Myrtillocactus sont propagées asexuellement par explants ou par clonage. En conditions contrôlées, la micropropagation in vitro d'explants apicaux et basaux donne de hauts rendements. Pour la culture, une attention particulière doit être apportée aux températures hivernales minimales. Description des fruits et caractérisation biochimique. Le fruit comestible est globulaire, avec un diamètre atteignant 1,5 cm. La pulpe est colloïdale, sa couleur va du rougeoyant au rouge bleuâtre. La caractérisation chimique a principalement porté sur la composition en bétalaines, dont les composés prédominants sont la bétanine et les bétaxanthines. La teneur en bétalaines serait de 2,3 mg ·100 g -1 de pulpe. Le colorant semble être plus stable que celui des betteraves rouges. Consommation humaine et importance commerciale. Pendant la saison de production (juin à septembre), les fruits sont trouvés sur tous les marchés de leur lieu de production. Ils sont mangés frais ou transformés. La commercialisation du fruit est limitée principalement aux zones de production rurales dans certains états du Mexique. Conclusions. Notre synthèse a établi que les informations publiées sur Myrtillocactus étaient rares et incomplètes. La plante est sous utilisée, en dépit de ses propriétés alimentaires et de son potentiel commercial. Comme les espèces de Myrtillocactus s'adaptent facilement dans des conditions de grande sécheresse, elles mériteraient de faire l'objet de beaucoup plus de recherche (Résumé d'auteur

The transmembrane protein bacterioopsin affects the polarity of the hydrophobic core of the host lipid bilayer

AbstractInfluence of the transmembrane protein bacterioopsin (the retinal-free form of bacteriorhodopsin) on the polarity of egg-phosphatidylcholine bilayers was studied by means of a steady-state and time-resolved fluorescence approach exploiting the solvatochromic properties of the 2-anthroyl fluorophore. Introduced in phosphatidylcholine molecules in the form of 8-(2-anthroyl)octanoic acid, this fluorophore probed the hydrocarbon core of the lipid bilayer. As previously shown (E. Pérochon et al., Biochemistry 31 (1992) 7672–7682), water molecules were detected in this region of the terminal part of the lipid acyl chains. Their number was considerably reduced upon addition of bacterioopsin to the lipids. This was assessed by a blue shift in the fluorescence emission spectra of the probe and a marked decrease in the fractional population of fluorophores interacting with water, to the benefit of those experiencing a hydrophobic environment. In agreement with current theories, this decrease in the hydration of the bilayer may be linked to an increase in the acyl chain order and a decrease in the lateral diffusion coefficient of lipids near the protein. The data obtained at high protein concentration accounts for a protein/lipid interface which is much less hydrated than the hydrophobic core of a protein-free lipid bilayer

Integration of nanostructured planar diffractive lenses dedicated to near infrared detection for CMOS image sensors

This paper deals with the integration of metallic and dielectric nanostructured planar lenses into a pixel from a silicon based CMOS image sensor, for a monochromatic application at 1.064 μm. The first is a Plasmonic Lens, based on the phase delay through nanoslits, which has been found to be hardly compatible with current CMOS technology and exhibits a notable metallic absorption. The second is a dielectric Phase-Fresnel Lens integrated at the top of a pixel, it exhibits an Optical Efficiency (OE) improved by a few percent and an angle of view of 50°. The third one is a metallic diffractive lens integrated inside a pixel, which shows a better OE and an angle of view of 24°. The last two lenses exhibit a compatibility with a spectral band close to 1.064 μm

Chikungunya: risks for travellers

RATIONALE FOR REVIEW Chikungunya outbreaks continue to occur, with changing epidemiology. Awareness about chikungunya is low both among the at-risk travellers and healthcare professionals, which can result in underdiagnosis and underreporting. This review aims to improve awareness among healthcare professionals regarding the risks of chikungunya for travellers. KEY FINDINGS Chikungunya virus transmission to humans occurs mainly via daytime-active mosquitoes, Aedes aegypti and Aedes albopictus. The areas where these mosquitoes live is continuously expanding, partly due to climate changes. Chikungunya is characterized by an acute onset of fever with joint pain. These symptoms generally resolve within 1-3 weeks, but at least one-third of the patients suffer from debilitating rheumatologic symptoms for months to years. Large outbreaks in changing regions of the world since the turn of the 21st century (e.g. Caribbean, La Réunion; currently Brazil, India) have resulted in growing numbers of travellers importing chikungunya, mainly to Europe and North America. Viremic travellers with chikungunya infection have seeded chikungunya clusters (France, United States of America) and outbreaks (Italy in 2007 and 2017) in non-endemic countries where Ae. albopictus mosquitoes are present. Community preventive measures are important to prevent disease transmission by mosquitoes. Individual preventive options are limited to personal protection measures against mosquito bites, particularly the daytime-active mosquitos that transmit the chikungunya virus. Candidate vaccines are on the horizon and regulatory authorities will need to assess environmental and host risk factors for persistent sequelae, such as obesity, age (over 40 years) and history of arthritis or inflammatory rheumatologic disease to determine which populations should be targeted for these chikungunya vaccines. CONCLUSIONS/RECOMMENDATIONS Travellers planning to visit destinations with active CHIKV circulation should be advised about the risk for chikungunya, prevention strategies, the disease manifestations, possible chronic rheumatologic sequelae and, if symptomatic, seek medical evaluation and report potential exposures