Draft Genome Sequence Of Burkholderia Andropogonis Type Strain Icmp2807, Isolated From Sorghum Bicolor.

Here, we report the draft genome sequence of Burkholderia andropogonis ICMP2807, a phytopathogenic bacterium isolated from Sorghum bicolor plants in the United States.

Polypodium leucotomos targets multiple aspects of oral carcinogenesis and it is a potential antitumor phytotherapy against tongue cancer growth

Peer reviewe

Heparanase 1 Upregulation Promotes Tumor Progression and Is a Predictor of Low Survival for Oral Cancer

Background: Oral cavity cancer is still an important public health problem throughout the world. Oral squamous cell carcinomas (OSCCs) can be quite aggressive and metastatic, with a low survival rate and poor prognosis. However, this is usually related to the clinical stage and histological grade, and molecular prognostic markers for clinical practice are yet to be defined. Heparanase (HPSE1) is an endoglycosidase associated with extracellular matrix remodeling, and although involved in several malignancies, the clinical implications of HPSE1 expression in OSCCs are still unknown.Methods: We sought to investigate HPSE1 expression in a series of primary OSCCs and further explore whether its overexpression plays a relevant role in OSCC tumorigenesis. mRNA and protein expression analyses were performed in OSCC tissue samples and cell lines. A loss-of-function strategy using shRNA and a gain-of-function strategy using an ORF vector targeting HPSE1 were employed to investigate the endogenous modulation of HPSE1 and its effects on proliferation, apoptosis, adhesion, epithelial-mesenchymal transition (EMT), angiogenesis, migration, and invasion of oral cancer in vitro.Results: We demonstrated that HPSE1 is frequently upregulated in OSCC samples and cell lines and is an unfavorable prognostic indicator of disease-specific survival when combined with advanced pT stages. Moreover, abrogation of HPSE1 in OSCC cells significantly promoted apoptosis and inhibited proliferation, migration, invasion, and epithelial-mesenchymal transition by significantly decreasing the expression of N-cadherin and vimentin. Furthermore, a conditioned medium of HPSE1-downregulated cells resulted in reduced vascular endothelial growth.Conclusion: Our results confirm the overexpression of HPSE1 in OSCCs, suggest that HPSE1 expression correlates with disease progression as it is associated with several important biological processes for oral tumorigenesis, and can be managed as a prognostic marker for patients with OSCC.Peer reviewe

POSSIBILIDADES DE CONSTRUÇÃO DE UM ESPAÇO PEDAGÓGICO E CULTURAL EMANCIPATÓRIO EM BIBLIOTECAS COMUNITÁRIAS NO CAMPO

“Literatura e Expressão cultural na Educação do Campo: Poesia, música, história e literatura camponesa – Afagos em tempos de pandemia” este podcast é produto do projeto Formação de educadores do campo: possibilidades de construção de um espaço pedagógico e cultural emancipatório em bibliotecas comunitárias no campo, vinculado ao Programa LICENCIAR da Pró – reitoria de Graduação e Educação profissional da Universidade Federal do Paraná, tendo com parceria o laboratório de estudos em Ludicidade, tecnologia e Comunicação (LUTECOM) da UFPR Litoral

Polypodium leucotomos targets multiple aspects of oral carcinogenesis and it is a potential antitumor phytotherapy against tongue cancer growth

Introduction: Oral cancer refers to malignant tumors, of which 90% are squamous cell carcinomas (OSCCs). These malignancies exhibit rapid progression, poor prognosis, and often mutilating therapeutical approaches. The determination of a prophylactic and/or therapeutic antitumor role of the polyphenolic extract Polypodium leucotomos(PL) would be relevant in developing new tools for prevention and treatment.Methods: We aimed to determine the antitumor effect of PL by treating OSCC cell lines with PL metabolites and evaluating its action during OSCC progression in vivo.Results: PL treatment successfully impaired cell cycling and proliferation, migration, and invasion, enhanced apoptosis, and modulated macrophage polarization associated with the tumoral immune-inflammatory response of tongue cancer cell lines (TSCC). PL treatment significantly decreased the expression of MMP1 (p < 0.01) and MMP2 (p < 0.001), and increased the expression of TIMP1 (p < 0.001) and TIMP2 (p < 0.0001) in these cells. The mesenchymal-epithelial transition phenotype was promoted in cells treated with PL, through upregulation of E-CAD (p < 0.001) and reduction of N-CAD (p < 0.05). PL restrained OSCC progression in vivo by inhibiting tumor volume growth and decreasing the number of severe dysplasia lesions and squamous cell carcinomas. Ki-67 was significantly higher expressed in tongue tissues of animals not treated with PL(p < 0.05), and a notable reduction in Bcl2 (p < 0.05) and Pcna (p < 0.05) cell proliferation-associated genes was found in dysplastic lesions and TSCCs of PL-treated mice. Finally, N-cad(Cdh2), Vim, and Twist were significantly reduced in tongue tissues treated with PL.Conclusion: PL significantly decreased OSCC carcinogenic processes in vitro and inhibited tumor progression in vivo. PL also appears to contribute to the modulation of immune-inflammatory oral tumor-associated responses. Taken together, these results suggest that PL plays an important antitumor role in processes associated with oral carcinogenesis and may be a potential phytotherapeutic target for the prevention and/or adjuvant treatment of TSCC

Educomunicação e suas áreas de intervenção: Novos paradigmas para o diálogo intercultural

oai:omp.abpeducom.org.br:publicationFormat/1O material aqui divulgado representa, em essência, a contribuição do VII Encontro Brasileiro de Educomunicação ao V Global MIL Week, da UNESCO, ocorrido na ECA/USP, entre 3 e 5 de novembro de 2016. Estamos diante de um conjunto de 104 papers executivos, com uma média de entre 7 e 10 páginas, cada um. Com este rico e abundante material, chegamos ao sétimo e-book publicado pela ABPEducom, em seus seis primeiros anos de existência. A especificidade desta obra é a de trazer as “Áreas de Intervenção” do campo da Educomunicação, colocando-as a serviço de uma meta essencial ao agir educomunicativo: o diálogo intercultural, trabalhado na linha do tema geral do evento internacional: Media and Information Literacy: New Paradigms for Intercultural Dialogue

Biochemical, genetic and pathogenic characterization of Burkholderia andropogonis and taxonomic reassessment of the species

Orientador: Suzete Aparecida Lanza DestéfanoTese (doutorado) - Universidade Estadual de Campinas, Instituto de BiologiaResumo: Burkholderia andropogonis, inicialmente descrita por Smith (1911) como Bacterium andropogonis, agente causal de doença em sorgo (Sorghum bicolor), causa sintomas como manchas e listras foliares em uma ampla gama de hospedeiros com extensa distribuição geográfica. Essa espécie bacteriana possui características únicas ausentes na maioria dos patógenos de planta, como a presença de um único flagelo polar do tipo embainhado e produção de metabólitos secundários do tipo rizobiotoxina. O objetivo desse estudo foi caracterizar a diversidade bioquímica, genética e patogênica de 42 linhagens de B. andropogonis e reavaliar a relação filogenética dessa espécie bacteriana dentro do gênero Burkholderia. Características bioquímicas e fisiológicas diferenciais não foram observadas entre as linhagens isoladas de diferentes hospedeiros e regiões geográficas, assim como não foi verificada especialização patogênica nos testes de patogenicidade em diferentes plantas hospedeiras. Na caracterização molecular, a técnica de rep-PCR mostrou-se um método sensível para medir a variação genética dentro desta espécie bacteriana, uma vez que a análise combinada utilizando REP-, ERIC- e BOX-PCR permitiu a separação das linhagens em grupos de acordo com seus hospedeiros específicos e/ou localização geográfica. Nas análises filogenéticas do gene RNA ribossomal 16S e de multilocus (MLSA) utilizando cinco genes housekeeping (gyrB, recA, lepA, atpD e gltB), B. andropogonis ficou separada das demais espécies do gênero, com baixas porcentagens de similaridade entre as sequências dos genes. O sequenciamento do genoma da linhagem Tipo de B. andropogonis também foi realizado nesse estudo e uma análise de multilocus utilizando 30 diferentes genes conservados mais uma vez mostrou que B. andropogonis permaneceu separada das demais espécies do gênero Burkholderia. Além disso, a identidade média de nucleotídeos (ANI), a frequência de tetranucleotídeos (TETRA) e o porcentual de proteínas conservadas (POCP) foram calculados e B. andropogonis apresentou valores mais baixos nas comparações aos pares com 12 diferentes espécies do gênero Burkholderia, reforçando a distância observada nas análises filogenéticas. Nossos resultados claramente colocaram B. andropogonis em um grupo distinto que pode representar um novo gêneroAbstract: Burkholderia andropogonis initially described by Smith (1911) as the causal agent of leaf stripe disease on sorghum (Sorghum bicolor), affects a wide range of host plants economically important with an extensive geographical distribution. This bacterial species has unique features absent in most plant pathogens such as a single polar sheathed flagellum and rhizobitoxine production. The aim of this study was to assess the genetic, biochemical and pathogenic diversity among Burkholderia andropogonis strains and to establish the phylogenetic relationship of this bacterial species within Burkholderia genus. Differential biochemical and physiological features were not observed among strains isolated from different host and different geographical origins, as well as host specialization was not observed in the pathogenicity tests on distinct plant species. In the molecular characterization the rep-PCR technique was a sensitive method to measure the genetic variation within this bacterial species once the combined analysis using REP, ERIC and BOX-PCR allowed separation of strains into clusters according to specific hosts and/or geographical location. . The phylogenetic analysis using 16S rRNA gene and multilocus sequence analysis (MLSA) using five housekeeping genes (gyrB, recA, lepA, atpD e gltB) allowed clear separation of B. andropogonis from the other species of the genus, with low percentages of similarity among the genes sequences. After the genome sequencing of B. andropogonis type strain a multilocus analysis using 30 different conserved genes was also performed and again, B. andropogonis remained separated from the species of the Burkholderia genus. In addition, the average nucleotide identity (ANI), tetranucleotide frequency signature (TETRA), and percentage of conserved proteins (POCP) analyses were also carried out, and B. andropogonis species showed lower values when compared to the other Burkholderia species, reinforcing the distance observed in the molecular analyses. Our findings clearly indicates that B. andropogonis belongs to a distinct group and may represent a new genusDoutoradoGenetica de MicroorganismosDoutora em Genética e Biologia Molecular2011/12222-2FAPES