Connectivity and seasonality cause rapid taxonomic and functional trait succession within an invertebrate community after stream restoration

General colonization concepts consent that a slow process of microhabitat formation and subsequent niche realization occurs during early stages after new habitat is released. Subsequently, only few species are able to colonize new habitat in the early onset of succession, while species richness increases steadily over time. Although most colonization studies have been performed in terrestrial ecosystems, running water ecosystems are equally or even more prone to colonization after disturbance due to their dynamic nature. We question how invertebrate succession patterns reconcile with general colonization concepts. With this study we provide insight into the colonization process in newly created lowland stream trajectories and answer how within-stream bio- and functional diversity develops over time. Our results show a rapid influx of species, with a wide range of functional traits, during the first season after water flow commenced. During more than two years of regular monitoring, immigration rates were highest in autumn, marking the effects of seasonality on invertebrate dispersal. Biodiversity increased while abundance peaks of species alternated between seasons. Moreover, also days since start of the experiment explains a considerable part of the variability for taxa as well as traits. However, the relative trait composition remained similar throughout the entire monitoring period and only few specific traits had significantly higher proportions during specific seasons. This indicates that first phase colonization in freshwater streams can be a very rapid process that results in a high biodiversity and a large variety of species functional characteristics from the early onset of succession, contradicting general terrestrial colonization theory.</p

Connectivity and seasonality cause rapid taxonomic and functional trait succession within an invertebrate community after stream restoration

General colonization concepts consent that a slow process of microhabitat formation and subsequent niche realization occurs during early stages after new habitat is released. Subsequently, only few species are able to colonize new habitat in the early onset of succession, while species richness increases steadily over time. Although most colonization studies have been performed in terrestrial ecosystems, running water ecosystems are equally or even more prone to colonization after disturbance due to their dynamic nature. We question how invertebrate succession patterns reconcile with general colonization concepts. With this study we provide insight into the colonization process in newly created lowland stream trajectories and answer how within-stream bio- and functional diversity develops over time. Our results show a rapid influx of species, with a wide range of functional traits, during the first season after water flow commenced. During more than two years of regular monitoring, immigration rates were highest in autumn, marking the effects of seasonality on invertebrate dispersal. Biodiversity increased while abundance peaks of species alternated between seasons. Moreover, also days since start of the experiment explains a considerable part of the variability for taxa as well as traits. However, the relative trait composition remained similar throughout the entire monitoring period and only few specific traits had significantly higher proportions during specific seasons. This indicates that first phase colonization in freshwater streams can be a very rapid process that results in a high biodiversity and a large variety of species functional characteristics from the early onset of succession, contradicting general terrestrial colonization theory.</p

Meettechnieken voor de Kennisimpuls Nutriëntenmaatregelen

Deze notitie is opgesteld als voorbereiding op het uitwerken van de meetstrategie in de pilotgebieden voor de Kennisimpuls Nutriëntenmaatregelen . Het doel van dit rapport is om een overzicht te geven van de meettechnieken tools die inzetbaar zijn voor het onderzoek naar de bronnen en routes van nutriënten. We richten ons daarbij op meettechnieken voor nutriënten in oppervlaktewater en in bodemvocht en grondwater

Total plasma proANP increases with atrial dilatation in horses

Equine atrial natriuretic peptide (ANP) plasma concentrations are correlated with left atrial size. However, species-specific assays are lacking and the results from human assays are poorly reproducible. A new methodology called processing independent analysis (PIA) that measures the total proANP product in plasma has proven to be successful in human medicine, but has never been used in horses. The aims were to establish an equine proANP reference interval by measurement of the total proANP product using PIA and to examine the proANP concentrations in horses with atrial dilatation. Sample stability was studied by comparison of storage at -80 degrees C and -20 degrees C. Plasma samples were obtained from 23 healthy horses, 12 horses with moderate or severe valvular regurgitation without atrial dilatation and 42 horses with valvular regurgitation and atrial dilatation. The proANP concentration was significantly (P<0.001) higher in horses with atrial dilatation (761.4 (442.1-1859.1) pmol/l) than in healthy horses (491.6 (429.5-765.9) pmol/l; P<0.001) or horses with cardiac disease but without atrial dilatation (544.4 (457.0-677.6) pmol/l). A cut-off value (573.8 pmol/l) for detection of atrial dilatation was calculated. Sample storage at -80 degrees C did not differ from sample storage at -20 degrees C. The measurement of total proANP in plasma detects atrial dilatation in horses and may be useful for clinical evaluation in equine medicine

Analytical validation of a high-sensitivity cardiac troponin T assay in horses

Although cardiac troponin T (cTnT) assays have been used to detect myocardial damage in horses, a cTnT assay has not been analytically validated, to our knowledge. The aims of this study were to estimate the precision of a high-sensitivity cTnT assay in horses and determine the effect of hemolysis on the measured cTnT concentration. Serum samples from horses were mixed in 3 different pools. Pool 1 consisted of samples from 3 healthy horses, pool 2 from 6 horses with heart failure or atypical myopathy, and pool 3 from 10 horses with atypical myopathy. The within- and between-run coefficients of variation were determined for each pool. Pools 2 and 3 were diluted to estimate linearity. To study the influence of sample hemolysis, serum was collected from 4 horses with a high cTnT concentration, in which hemolysis was mechanically induced. In addition, ethylenediamine tetra-acetic acid blood tubes were collected from 3 other horses, from which hemolysate was prepared and added to plasma at different concentrations. The within- and between-run coefficients of variation of all pools were <10%, and a good linearity was found. Three out of 4 hemolyzed serum samples had a decreased serum cTnT concentration. Plasma samples with a high hemolysis index showed a negative interference, resulting in a lower cTnT concentration. Results of the high-sensitivity cTnT assay were highly reproducible. Because samples from horses with musculoskeletal damage were included, further studies should test the possible cross-reactivity between troponin T of musculoskeletal and cardiac origin before the assay can be used in equine clinical practice

Changes in abundance (log (# individuals)) per taxonomical macroinvertebrate class over time (# days since start).

<p>Insects form the largest class of colonist from the early onset of succession, maintaining equal levels of large abundance over time.</p

Morphological, physical and chemical characteristics of three re-connected stream trajectories in the Leuvenumse stream, the Netherlands.

<p>These parameters were measured 18 times from September 2014 until November 2016 within each trajectory, adjacent to the streambed surface where the invertebrate samples were taken.</p

Changes in functional trait composition (relative trait presence) over time of six different trait categories.

<p>Linear regressions of functional traits over time were calculated to test if specific functional traits were of increasing or decreasing importance during succession. Only taxa presence/absence data was considered in this case, multiplied with specific trait values and divided by the total number of species present per moment. Regression lines and coefficient are only shown for significant regressions. A: Feeding group, significant regressions: shredder, active filter feeder, passive filter feeder, B: Locomotion type, sig. regr.: burrowing, C: Dispersal mode, sig. regr.: aerial passive, aerial active, D: Rarity, sig. regr: semi-rare, E: Reproductive cycle, sig. regr: semivoltine, univoltine, F: r-K selection strategy, no sig. regr.</p