4,930 research outputs found
High energy proton radiation damage to (AlGa)As-G aAs solar cells
Twelve 2 + 2 sq cm (AlGa)As-GaAs solar cells were fabricated and were subjected to 15.4 and 40 MeV of proton irradiation. The results showed that the GaAs cells degrade considerably less than do conventional and developmental K7 silicon cells. The detailed characteristics of the GaAs and silicon cells, both before and after irradiation, are described. Further optimization of the GaAs cells seems feasible, and areas for future work are suggested
Pyrophosphate-Dependent ATP Formation from Acetyl Coenzyme A in Syntrophus aciditrophicus, a New Twist on ATP Formation.
UnlabelledSyntrophus aciditrophicus is a model syntrophic bacterium that degrades key intermediates in anaerobic decomposition, such as benzoate, cyclohexane-1-carboxylate, and certain fatty acids, to acetate when grown with hydrogen-/formate-consuming microorganisms. ATP formation coupled to acetate production is the main source for energy conservation by S. aciditrophicus However, the absence of homologs for phosphate acetyltransferase and acetate kinase in the genome of S. aciditrophicus leaves it unclear as to how ATP is formed, as most fermentative bacteria rely on these two enzymes to synthesize ATP from acetyl coenzyme A (CoA) and phosphate. Here, we combine transcriptomic, proteomic, metabolite, and enzymatic approaches to show that S. aciditrophicus uses AMP-forming, acetyl-CoA synthetase (Acs1) for ATP synthesis from acetyl-CoA. acs1 mRNA and Acs1 were abundant in transcriptomes and proteomes, respectively, of S. aciditrophicus grown in pure culture and coculture. Cell extracts of S. aciditrophicus had low or undetectable acetate kinase and phosphate acetyltransferase activities but had high acetyl-CoA synthetase activity under all growth conditions tested. Both Acs1 purified from S. aciditrophicus and recombinantly produced Acs1 catalyzed ATP and acetate formation from acetyl-CoA, AMP, and pyrophosphate. High pyrophosphate levels and a high AMP-to-ATP ratio (5.9 ± 1.4) in S. aciditrophicus cells support the operation of Acs1 in the acetate-forming direction. Thus, S. aciditrophicus has a unique approach to conserve energy involving pyrophosphate, AMP, acetyl-CoA, and an AMP-forming, acetyl-CoA synthetase.ImportanceBacteria use two enzymes, phosphate acetyltransferase and acetate kinase, to make ATP from acetyl-CoA, while acetate-forming archaea use a single enzyme, an ADP-forming, acetyl-CoA synthetase, to synthesize ATP and acetate from acetyl-CoA. Syntrophus aciditrophicus apparently relies on a different approach to conserve energy during acetyl-CoA metabolism, as its genome does not have homologs to the genes for phosphate acetyltransferase and acetate kinase. Here, we show that S. aciditrophicus uses an alternative approach, an AMP-forming, acetyl-CoA synthetase, to make ATP from acetyl-CoA. AMP-forming, acetyl-CoA synthetases were previously thought to function only in the activation of acetate to acetyl-CoA
Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs). CXLs afford consolidated advantages not previously available in a simple cross-linker, including (1) their small size and cationic nature at physiological pH, resulting in good water solubility and cell-permeability, (2) an alkyne group for bio-orthogonal conjugation to affinity tags via the click reaction for enrichment of cross-linked peptides, (3) a nucleophilic displacement reaction involving the 1,2,3-triazole ring formed in the click reaction, yielding a lock-mass reporter ion for only clicked peptides, and (4) higher charge states of cross-linked peptides in the gas-phase for augmented electron transfer dissociation (ETD) yields. Ubiquitin, a lysine-abundant protein, is used as a model system to demonstrate structural studies using CXLs. To validate the sensitivity of our approach, biotin-azide labeling and subsequent enrichment of cross-linked peptides are performed for cross-linked ubiquitin digests mixed with yeast cell lysates. Cross-linked peptides are detected and identified by collision induced dissociation (CID) and ETD with linear quadrupole ion trap (LTQ)-Fourier transform ion cyclotron resonance (FTICR) and LTQ-Orbitrap mass spectrometers. The application of CXLs to more complex systems (e.g., in vivo cross-linking) is illustrated by Western blot detection of Cul1 complexes including known binders, Cand1 and Skp2, in HEK 293 cells, confirming good water solubility and cell-permeability
Rigorous Real-Time Feynman Path Integral for Vector Potentials
we will show the existence and uniqueness of a real-time, time-sliced Feynman
path integral for quantum systems with vector potential. Our formulation of the
path integral will be derived on the transition probability amplitude via
improper Riemann integrals. Our formulation will hold for vector potential
Hamiltonian for which its potential and vector potential each carries at most a
finite number of singularities and discontinuities
Strain Hardening of Polymer Glasses: Entanglements, Energetics, and Plasticity
Simulations are used to examine the microscopic origins of strain hardening
in polymer glasses. While stress-strain curves for a wide range of temperature
can be fit to the functional form predicted by entropic network models, many
other results are fundamentally inconsistent with the physical picture
underlying these models. Stresses are too large to be entropic and have the
wrong trend with temperature. The most dramatic hardening at large strains
reflects increases in energy as chains are pulled taut between entanglements
rather than a change in entropy. A weak entropic stress is only observed in
shape recovery of deformed samples when heated above the glass transition.
While short chains do not form an entangled network, they exhibit partial shape
recovery, orientation, and strain hardening. Stresses for all chain lengths
collapse when plotted against a microscopic measure of chain stretching rather
than the macroscopic stretch. The thermal contribution to the stress is
directly proportional to the rate of plasticity as measured by breaking and
reforming of interchain bonds. These observations suggest that the correct
microscopic theory of strain hardening should be based on glassy state physics
rather than rubber elasticity.Comment: 15 pages, 12 figures: significant revision
A rigorous real time Feynman Path Integral and Propagator
We will derive a rigorous real time propagator for the Non-relativistic
Quantum Mechanic transition probability amplitude and for the
Non-relativistic wave function. The propagator will be explicitly given in
terms of the time evolution operator. The derivation will be for all
self-adjoint nonvector potential Hamiltonians. For systems with potential that
carries at most a finite number of singularity and discontinuities, we will
show that our propagator can be written in the form of a rigorous real time,
time sliced Feynman path integral via improper Riemann integrals. We will also
derive the Feynman path integral in Nonstandard Analysis Formulation. Finally,
we will compute the propagator for the harmonic oscillator using the
Nonstandard Analysis Feynman path integral formuluation; we will compute the
propagator without using any knowledge of classical properties of the harmonic
oscillator
Understanding Shoot and Root Development
Shoot and root development of the grass tiller is presented as a series of events on the tiller axis. Leaf, tiller bud, true stem, and root development are successive events in the life cycle of a single phytomer and the tiller is a co-ordinated series of phytomers, successive phytomers being progressively more advanced than the previous phytomer. In reviewing the individual growth processes of leaf, tiller, true stem, and root formation, fundamental determinants of light and nutrient capture are examined and examples presented to illustrate the link between component processes, plant morphogenesis, and plant performance. An example of the application of this understanding in plant improvement is given
LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA.
The DExD/H box RNA helicases retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation associated gene-5 (mda-5) sense viral RNA in the cytoplasm of infected cells and activate signal transduction pathways that trigger the production of type I interferons (IFNs). Laboratory of genetics and physiology 2 (LGP2) is thought to influence IFN production by regulating the activity of RIG-I and mda-5, although its mechanism of action is not known and its function is controversial. Here we show that expression of LGP2 potentiates IFN induction by polyinosinic-polycytidylic acid [poly(I:C)], commonly used as a synthetic mimic of viral dsRNA, and that this is particularly significant at limited levels of the inducer. The observed enhancement is mediated through co-operation with mda-5, which depends upon LGP2 for maximal activation in response to poly(I:C). This co-operation is dependent upon dsRNA binding by LGP2, and the presence of helicase domain IV, both of which are required for LGP2 to interact with mda-5. In contrast, although RIG-I can also be activated by poly(I:C), LGP2 does not have the ability to enhance IFN induction by RIG-I, and instead acts as an inhibitor of RIG-I-dependent poly(I:C) signaling. Thus the level of LGP2 expression is a critical factor in determining the cellular sensitivity to induction by dsRNA, and this may be important for rapid activation of the IFN response at early times post-infection when the levels of inducer are low
Village Baseline Study: Site Analysis Report for Xai Xai, Zongoene Village, Bairro 1, Mozambique
This is the report of the village baseline study of Zongoene Village, Bairro 1, in the CCAFS benchmark site of Xai Xai, Mozambique in May 2013 to complement an earlier household baseline survey done in the same village. Zongoene village is located by the coast at the mouth of the Limpopo River where there are extensive wetlands along with forests and sand dunes. The village is divided into five sections: Bairro 1, Bairro 2, Bairro 3, Bairro 4 and Bairro 5. Zongoene is the result of a government resettlement program that brought people together to ease service provision. Nonetheless, the level and quality of services remain very low with roads in bad condition, local schools poorly built and lacking water, one hospital with insufficient staff and resources, and nascent electrical availability. The concentrated and expanding population is also increasing pressures on the environment as demand for food and farmland grows
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