1,166 research outputs found
Flavonoids and ω3-polyunsaturated fatty acid supplementation in renal transplant recipients: new arguments from COVID-19
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Multimodal Treatment Eliminates Cancer Stem Cells and Leads to Long-Term Survival in Primary Human Pancreatic Cancer Tissue Xenografts.
Copyright: 2013 Hermann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.PURPOSE: In spite of intense research efforts, pancreatic ductal adenocarcinoma remains one of the most deadly malignancies in the world. We and others have previously identified a subpopulation of pancreatic cancer stem cells within the tumor as a critical therapeutic target and additionally shown that the tumor stroma represents not only a restrictive barrier for successful drug delivery, but also serves as a paracrine niche for cancer stem cells. Therefore, we embarked on a large-scale investigation on the effects of combining chemotherapy, hedgehog pathway inhibition, and mTOR inhibition in a preclinical mouse model of pancreatic cancer. EXPERIMENTAL DESIGN: Prospective and randomized testing in a set of almost 200 subcutaneous and orthotopic implanted whole-tissue primary human tumor xenografts. RESULTS: The combined targeting of highly chemoresistant cancer stem cells as well as their more differentiated progenies, together with abrogation of the tumor microenvironment by targeting the stroma and enhancing tissue penetration of the chemotherapeutic agent translated into significantly prolonged survival in preclinical models of human pancreatic cancer. Most pronounced therapeutic effects were observed in gemcitabine-resistant patient-derived tumors. Intriguingly, the proposed triple therapy approach could be further enhanced by using a PEGylated formulation of gemcitabine, which significantly increased its bioavailability and tissue penetration, resulting in a further improved overall outcome. CONCLUSIONS: This multimodal therapeutic strategy should be further explored in the clinical setting as its success may eventually improve the poor prognosis of patients with pancreatic ductal adenocarcinoma
Many-core applications to online track reconstruction in HEP experiments
Interest in parallel architectures applied to real time selections is growing
in High Energy Physics (HEP) experiments. In this paper we describe performance
measurements of Graphic Processing Units (GPUs) and Intel Many Integrated Core
architecture (MIC) when applied to a typical HEP online task: the selection of
events based on the trajectories of charged particles. We use as benchmark a
scaled-up version of the algorithm used at CDF experiment at Tevatron for
online track reconstruction - the SVT algorithm - as a realistic test-case for
low-latency trigger systems using new computing architectures for LHC
experiment. We examine the complexity/performance trade-off in porting existing
serial algorithms to many-core devices. Measurements of both data processing
and data transfer latency are shown, considering different I/O strategies
to/from the parallel devices.Comment: Proceedings for the 20th International Conference on Computing in
High Energy and Nuclear Physics (CHEP); missing acks adde
GPU-based Real-time Triggering in the NA62 Experiment
Over the last few years the GPGPU (General-Purpose computing on Graphics
Processing Units) paradigm represented a remarkable development in the world of
computing. Computing for High-Energy Physics is no exception: several works
have demonstrated the effectiveness of the integration of GPU-based systems in
high level trigger of different experiments. On the other hand the use of GPUs
in the low level trigger systems, characterized by stringent real-time
constraints, such as tight time budget and high throughput, poses several
challenges. In this paper we focus on the low level trigger in the CERN NA62
experiment, investigating the use of real-time computing on GPUs in this
synchronous system. Our approach aimed at harvesting the GPU computing power to
build in real-time refined physics-related trigger primitives for the RICH
detector, as the the knowledge of Cerenkov rings parameters allows to build
stringent conditions for data selection at trigger level. Latencies of all
components of the trigger chain have been analyzed, pointing out that
networking is the most critical one. To keep the latency of data transfer task
under control, we devised NaNet, an FPGA-based PCIe Network Interface Card
(NIC) with GPUDirect capabilities. For the processing task, we developed
specific multiple ring trigger algorithms to leverage the parallel architecture
of GPUs and increase the processing throughput to keep up with the high event
rate. Results obtained during the first months of 2016 NA62 run are presented
and discussed
Toll-like receptor kinetics in septic shock patients: a preliminary study.
The aim of this study is to evaluate some inflammatory parameter changes in septic shock patients and their possible correlation with clinical outcome, in particular when continuous veno-venous hemofiltration (CVVH) treatment is required. Considering the objective difficulty in enrolling this kind of patient, a preliminary study was initiated on seventeen septic shock patients admitted to a medical and surgical ICU. The mRNA expression of Toll-like receptor (TLR)-1, TLR-2, TLR-4, TLR-5, TLR-9, TNFalpha, IL-8 and IL-1beta was assessed, the plasmatic concentrations of IL-18, IL-2, IL-10 and TNFalpha were measured on the day of sepsis diagnosis and after 72 h. In those patients who developed acute renal failure unresponsive to medical treatment and who underwent CVVH treatment the same parameters were measured every 24 h during CVVH and after completion of the treatment. On sepsis diagnosis, gene expression of TLRs was up-regulated compared to the housekeeping gene in all the patients. After 72 h, in 35% of the patients a down-regulation of these genes was found compared to day 1, but it was not associated with a reduction of cytokine serum levels or improved clinical signs, better outcome or reduced mortality. After high volume hemofiltration treatment, cytokine serum levels and TLR expression were not significantly modified. In conclusion, considering the not numerous number of cases, from our preliminary study, we cannot certainly correlate TLR over-expression in septic shock patients with severity or outcome scores
NaNet: a Low-Latency, Real-Time, Multi-Standard Network Interface Card with GPUDirect Features
While the GPGPU paradigm is widely recognized as an effective approach to
high performance computing, its adoption in low-latency, real-time systems is
still in its early stages.
Although GPUs typically show deterministic behaviour in terms of latency in
executing computational kernels as soon as data is available in their internal
memories, assessment of real-time features of a standard GPGPU system needs
careful characterization of all subsystems along data stream path.
The networking subsystem results in being the most critical one in terms of
absolute value and fluctuations of its response latency.
Our envisioned solution to this issue is NaNet, a FPGA-based PCIe Network
Interface Card (NIC) design featuring a configurable and extensible set of
network channels with direct access through GPUDirect to NVIDIA Fermi/Kepler
GPU memories.
NaNet design currently supports both standard - GbE (1000BASE-T) and 10GbE
(10Base-R) - and custom - 34~Gbps APElink and 2.5~Gbps deterministic latency
KM3link - channels, but its modularity allows for a straightforward inclusion
of other link technologies.
To avoid host OS intervention on data stream and remove a possible source of
jitter, the design includes a network/transport layer offload module with
cycle-accurate, upper-bound latency, supporting UDP, KM3link Time Division
Multiplexing and APElink protocols.
After NaNet architecture description and its latency/bandwidth
characterization for all supported links, two real world use cases will be
presented: the GPU-based low level trigger for the RICH detector in the NA62
experiment at CERN and the on-/off-shore data link for KM3 underwater neutrino
telescope
Progress and status of APEmille
We report on the progress and status of the APEmille project: a SIMD parallel
computer with a peak performance in the TeraFlops range which is now in an
advanced development phase. We discuss the hardware and software architecture,
and present some performance estimates for Lattice Gauge Theory (LGT)
applications.Comment: Talk presented at LATTICE97, 3 pages, Late
apeNEXT: A multi-TFlops Computer for Simulations in Lattice Gauge Theory
We present the APE (Array Processor Experiment) project for the development
of dedicated parallel computers for numerical simulations in lattice gauge
theories. While APEmille is a production machine in today's physics simulations
at various sites in Europe, a new machine, apeNEXT, is currently being
developed to provide multi-Tflops computing performance. Like previous APE
machines, the new supercomputer is largely custom designed and specifically
optimized for simulations of Lattice QCD.Comment: Poster at the XXIII Physics in Collisions Conference (PIC03),
Zeuthen, Germany, June 2003, 3 pages, Latex. PSN FRAP15. Replaced for adding
forgotten autho
A simple method for generating full length cDNA from low abundance partial genomic clones
BACKGROUND: PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon boundaries. For these situations, the generation of full-length complementary DNAs from two partial genomic clones becomes necessary for the family of low abundance genes. RESULTS: The first approach we used for the isolation of full-length cDNA from two known genomic clones of Hox genes was based on fusion PCR. Here we describe a simple and efficient method of amplification for homeobox D13 (HOXD13) full length cDNA from two partial genomic clones. Specific 5' and 3' untranslated region (UTR) primer pairs and website program (primer3_www.cgv0.2) were key steps involved in this process. CONCLUSIONS: We have devised a simple, rapid and easy method for generating cDNA clone from genomic sequences. The full length HOXD13 clone (1.1 kb) generated with this technique was confirmed by sequence analysis. This simple approach can be utilized to generate full-length cDNA clones from available partial genomic sequences
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