15 research outputs found
Trace metal applications in atmospheric and watershed dynamics: Case studies of mercury deposition in New England and bedrock groundwater-surface water mixing
The studies presented in this dissertation focus on the environmental chemistry of two trace metals, mercury (Hg) and strontium (Sr). Both are naturally occurring and exist in the environment at trace levels.
Chapters II-IV of this dissertation focus on understanding the atmospheric chemistry of Hg and the wet and dry deposition of this toxic element. Chapter II presents results from Hg wet deposition measurements and ambient reactive gaseous Hg (RGM) measurements collected at Thompson Farm located in Durham, NH over a 3 year time period. The duration of this study allowed for seasonal and inter-annual comparisons. Seasonally, Hg wet deposition was greatest in the summer and spring and lowest in the winter and fall. Evidence of ineffective scavenging of RGM is provided due to the less frequent depletion of RGM during winter precipitation events in comparison with other seasons. RGM dry deposition estimates based on real time concentration measurements are greatest during the winter and spring. Ratios of the seasonal Hg wet deposition to RGM dry deposition vary greatly from 1.6 to 80.
A comparison between Hg wet deposition at Thompson Farm and a marine site, Appledore Island, is included in Chapter III. There were no significant differences in event concentration or deposition between the two sites, however, the sample collection efficiency varied greatly between the sites and may effect the results. Additionally, major ion concentrations were measured at the Appledore Island site and compared to the Hg concentrations. The analytical results coupled with air mass back trajectories suggest that the greatest amount of Hg wet deposition occurs when polluted continental air mixes with marine air.
A new filter extraction method for determining the environmentally mobile Hg concentration in bulk aerosol filters is presented in Chapter IV. This method is applied during a 2 week intensive sampling campaign at Appledore Island during summer 2009.
Chapter V explores the use of Sr isotope ratios to determine groundwater inputs to the Lamprey River. The groundwater and surface waters in the watershed exhibit large differences in 87Sr/86Sr indicating this geochemical indicator could be a useful tool in hydrogeologic studies of the watershed
Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-β-xylanase identified in a microbial consortium
Biotechnologies that aim to produce renewable fuels, chemicals, and bioproducts from residual ligno(hemi)cellulosic biomass mostly rely on enzymatic depolymerization of plant cell walls (PCW). This process requires an arsenal of diverse enzymes, including xylanases, which synergistically act on the hemicellulose, reducing the long and complex xylan chains to oligomers and simple sugars. Thus, xylanases play a crucial role in PCW depolymerization. Until recently, the largest xylanase family, glycoside hydrolase family 11 (GH11) has been exclusively represented by endo-catalytic β-1,4- and β-1,3-xylanases. Analysis of a metatranscriptome library from a microbial lignocellulose community resulted in the identification of an unusual exo-acting GH11 β-1,4-xylanase (MetXyn11). Detailed characterization has been performed on recombinant MetXyn11 including determination of its low-resolution small angle Xray scattering (SAXS) molecular envelope in solution. Our results reveal that MetXyn11 is a monomeric globular enzyme that liberates xylobiose from heteroxylans as the only product. MetXyn11 has an optimal activity in a pH range from 6 to 9 and an optimal temperature of 50 oC. The enzyme maintained above 65% of its original activity in the pH range 5 to 6 after being incubated for 72 h at 50 oC. Addition of the enzyme to a commercial enzymatic cocktail (CelicCtec3) promoted a significant increase of enzymatic hydrolysis yields of hydrothermally pretreated sugarcane bagasse (16% after 24 h of hydrolysis)
Association of water arsenic with incident diabetes in U.S. adults: The Multi-Ethnic Study of Atherosclerosis and The Strong Heart Study
Objective: We examined the association of arsenic in federally regulated community water systems (CWS) and unregulated private wells with type 2 diabetes (T2D) incidence in the Strong Heart Family Study (SHFS), a prospective study of American Indian communities, and the Multi-Ethnic Study of Atherosclerosis (MESA), a prospective study of racially/ethnically diverse urban U.S. communities.Research Design and Methods: We evaluated N=1,791 participants from SHFS and N=5,777 participants from MESA with water arsenic estimates available and free of T2D at baseline (2001-2003 and 2000-2002, respectively). Participants were followed for incident T2D until 2010 (SHFS) or 2019 (MESA). We used Cox proportional hazards mixed-effects models to account for clustering by family and residential zip code, with adjustment for sex, baseline age, body mass index (BMI), smoking status, and education.Results: T2D incidence was 24.4 cases per 1,000 person-years (mean follow-up 5.6 years) in SHFS and 11.2 per 1,000 person-years (mean follow-up 6.0 years) in MESA. In a meta-analysis across SHFS and MESA, the hazard ratio (95% confidence interval) per doubling in CWS arsenic was 1.10 (95%CI 1.02, 1.18). The corresponding hazard ratio was 1.09 (0.95, 1.26) in SHFS and 1.10 (1.01, 1.20) in MESA. The corresponding hazard ratio (95%CI) for arsenic in private wells and incident T2D in SHFS was 1.05 (0.95, 1.16). We observed statistical interaction and larger magnitude hazard ratios for participants with BMI Conclusions: Low to moderate water arsenic levels (<10 µg/L) were associated with T2D incidence in the SHFS and MESA.</p
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H2AX Prevents DNA Breaks from Progressing to Chromosome Breaks and Translocations
Histone H2AX promotes DNA double-strand break (DSB) repair and immunoglobulin heavy chain (IgH) class switch recombination (CSR) in B-lymphocytes. CSR requires activation-induced cytidine deaminase (AID) and involves joining of DSB intermediates by end joining. We find that AID-dependent IgH locus chromosome breaks occur at high frequency in primary H2AX-deficient B cells activated for CSR and that a substantial proportion of these breaks participate in chromosomal translocations. Moreover, activated B cells deficient for ATM, 53BP1, or MDC1, which interact with H2AX during the DSB response, show similarly increased IgH locus breaks and translocations. Thus, our findings implicate a general role for these factors in promoting end joining and thereby preventing DSBs from progressing into chromosomal breaks and translocations. As cellular p53 status does not markedly influence the frequency of such events, our results also have implications for how p53 and the DSB response machinery cooperate to suppress generation of lymphomas with oncogenic translocations
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Arsenic in private well water and birth outcomes in the United States.
BackgroundPrenatal exposure to drinking water with arsenic concentrations >50 μg/L is associated with adverse birth outcomes, with inconclusive evidence for concentrations ≤50 μg/L. In a collaborative effort by public health experts, hydrologists, and geologists, we used published machine learning model estimates to characterize arsenic concentrations in private wells-federally unregulated for drinking water contaminants-and evaluated associations with birth outcomes throughout the conterminous U.S.MethodsUsing several machine learning models, including boosted regression trees (BRT) and random forest classification (RFC), developed from measured groundwater arsenic concentrations of ∼20,000 private wells, we characterized the probability that arsenic concentrations occurred within specific ranges in groundwater. Probabilistic model estimates and private well usage data were linked by county to all live birth certificates from 2016 (n = 3.6 million). We evaluated associations with gestational age and term birth weight using mixed-effects models, adjusted for potential confounders and incorporated random intercepts for spatial clustering.ResultsWe generally observed inverse associations with term birth weight. For instance, when using BRT estimates, a 10-percentage point increase in the probability that private well arsenic concentrations exceeded 5 μg/L was associated with a -1.83 g (95% CI: -3.30, -0.38) lower term birth weight after adjusting for covariates. Similarly, a 10-percentage point increase in the probability that private well arsenic concentrations exceeded 10 μg/L was associated with a -2.79 g (95% CI: -4.99, -0.58) lower term birth weight. Associations with gestational age were null.ConclusionIn this largest epidemiologic study of arsenic and birth outcomes to date, we did not observe associations of modeled arsenic estimates in private wells with gestational age and found modest inverse associations with term birth weight. Study limitations may have obscured true associations, including measurement error stemming from a lack of individual-level information on primary water sources, water arsenic concentrations, and water consumption patterns
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High-throughput small molecule screening reveals Nrf2-dependent and -independent pathways of cellular stress resistance
Aging is the dominant risk factor for most chronic diseases. Development of antiaging interventions offers the promise of preventing many such illnesses simultaneously. Cellular stress resistance is an evolutionarily conserved feature of longevity. Here, we identify compounds that induced resistance to the superoxide generator paraquat (PQ), the heavy metal cadmium (Cd), and the DNA alkylator methyl methanesulfonate (MMS). Some rescue compounds conferred resistance to a single stressor, while others provoked multiplex resistance. Induction of stress resistance in fibroblasts was predictive of longevity extension in a published large-scale longevity screen in
, although not in testing performed in worms and flies with a more restricted set of compounds. Transcriptomic analysis and genetic studies implicated Nrf2/SKN-1 signaling in stress resistance provided by two protective compounds, cardamonin and AEG 3482. Small molecules identified in this work may represent attractive tools to elucidate mechanisms of stress resistance in mammalian cells