Nucleation in dilute 3He-4He liquid mixtures at low temperatures

We present a study of phase separation from supersaturated 3He-4He liquid mixtures at low temperatures addressing both the degree of critical supersaturation Dx and the thermal-to-quantum crossover temperature T* for the nucleation process. Two different nucleation seeds are investigated, namely 3He droplets and 4He vortex lines with cores filled with 3He. We have found that the experimental T* is reproduced when we consider that nucleation proceeds from 3He droplets, whereas Dx is reproduced when we consider 4He vortex lines filled with 3He. However, neither nucleation configuration is able to simultaneously reproduce the current experimental information on Dx and T*.Comment: To appear in J. of Low Temp. Physic

Cost-effective use of liquid nitrogen in cryogenic wind tunnels, phase 2

Cryogenic seal tests were performed and Rulon A was selected for the subject nutating positive displacement expander. A four-chamber expander was designed and fabricated. A nitrogen reliquefier flow system was also designed and constructed for testing the cold expander. Initial tests were unsatisfactory because of high internal friction attributed to nutating Rulon inlet and outlet valve plates. Replacement of the nutating valves with cam-actuated poppet valves improved performance. However, no net nitrogen reliquefaction was achieved due to high internal friction. Computer software was developed for accurate calculation of nitrogen reliquefaction from a system such as that proposed. These calculations indicated that practical reliquefaction rates of 15 to 19 percent could be obtained. Due to mechanical problems, the nutating expander did not demonstrate its feasibility nor that of the system. It was concluded that redesign and testing of a smaller nutating expander was required to prove concept feasibility

Architecture and engineering of a supramolecular functional material by manipulating the nano-structure of fiber network

Three-dimensional fiber networks were created from an organogel system consisting mainly of elongated fibrils by using a nonionic surfactant as an additive. The presence of the surfactant molecules manipulates the network structure by enhancing the mismatch nucleation on the growing fiber tips. Both the fiber network structure and the rheological properties of the material can be finely tuned by changing the surfactant concentration, which provides a robust approach to the engineering of supramolecular soft functional materials.<br /

A Test of Predator Avoidance by Larvae of the Blue Ridge Two-lined Salamander (Eurycea wilderae) in Appalachian Streams

Larval Blue Ridge Two-lined Salamanders (Eurycea wilderae) are significant components of Appalachian streams, reaching densities up to 100/m2. Not surprisingly, these salamanders fall prey to many types of predator. In order to test the hypothesis that larval E. wilderae actively avoid predators, we paired them against a variety of predators of this species. Predators included Banded Sculpins (Cottus carolinae), Chattahoochee Crayfish (Cambarus howardi), and Spring Salamanders (Gyrinophilus porphyriticus), both adult and larval. We placed larvae in a 1-m metal trough filled with water with a predator placed in a cage at one end. A control treatment consisted of an empty cage. For each trial, we placed a larval E. wilderae. After the larva stopped in one place for 10 min, we measured the distance between the larva and the cage. We ran 18‒20 replicates for each of the five treatments. A Kruskal-Wallis test showed no significant difference between any of the treatments in mean distance. Disagreement between our results and those of other workers suggests the possibility of interspecific or interpopulation variation in anti-predator behavior. In addition, because of their high densities and wide variety of predators, larval E. wilderae may not suffer sufficient predatory pressure from a particular species of predator to evolve appropriate behavioral responses

Superoxide reductase as a unique defense system against superoxide stress in the microaerophile Treponema pallidum.

International audienceAerobic life requires the presence of antioxidant enzymes, such as superoxide dismutase, catalase, and peroxidase to eliminate deleterious oxygen derivatives. Treponema pallidum, a microaerophilic bacterium responsible for venereal syphilis, is an interesting organism because it lacks all of the above-mentioned enzymes, as deduced from its recently sequenced genome. In this paper, we describe a gene in T. pallidum with sequence homologies to a new class of antioxidant systems, named superoxide reductases, recently isolated from sulfate-reducing bacteria (Lombard, M., Fontecave, M., Touati, D., and Nivière, V. (2000) J. Biol. Chem. 275, 115-121). We report that (i) expression of the T. pallidum gene fully restored to a superoxide dismutase-deficient Escherichia coli mutant the ability to grow under aerobic conditions; (ii) the corresponding protein displays a strong superoxide reductase activity; and (iii) the T. pallidum protein contains only one mononuclear nonheme ferrous center, able to reduce superoxide selectively and efficiently, whereas previously characterized superoxide reductase from Desulfoarculus baarsii contains an additional rubredoxin-like ferric center. These results suggest that T. pallidum antioxidant defenses rely on a new class of superoxide reductase and raise the question of the importance of superoxide reductases in mechanisms for detoxifying superoxide radicals

Events, processes, and the time of a killing

The paper proposes a novel solution to the problem of the time of a killing (ToK), which persistently besets theories of act-individuation. The solution proposed claims to expose a crucial wrong-headed assumption in the debate, according to which ToK is essentially a problem of locating some event that corresponds to the killing. The alternative proposal put forward here turns on recognizing a separate category of dynamic occurents, viz. processes. The paper does not aim to mount a comprehensive defense of process ontology, relying instead on extant defenses. The primary aim is rather to put process ontology to work in diagnosing the current state of play over ToK, and indeed in solving it

Reaction of the desulfoferrodoxin from Desulfoarculus baarsii with superoxide anion. Evidence for a superoxide reductase activity.

International audienceDesulfoferrodoxin is a small protein found in sulfate-reducing bacteria that contains two independent mononuclear iron centers, one ferric and one ferrous. Expression of desulfoferrodoxin from Desulfoarculus baarsii has been reported to functionally complement a superoxide dismutase deficient Escherichia coli strain. To elucidate by which mechanism desulfoferrodoxin could substitute for superoxide dismutase in E. coli, we have purified the recombinant protein and studied its reactivity toward O-(2). Desulfoferrodoxin exhibited only a weak superoxide dismutase activity (20 units mg(-1)) that could hardly account for its antioxidant properties. UV-visible and electron paramagnetic resonance spectroscopy studies revealed that the ferrous center of desulfoferrodoxin could specifically and efficiently reduce O-(2), with a rate constant of 6-7 x 10(8) M(-1) s(-1). In addition, we showed that membrane and cytoplasmic E. coli protein extracts, using NADH and NADPH as electron donors, could reduce the O-(2) oxidized form of desulfoferrodoxin. Taken together, these results strongly suggest that desulfoferrodoxin behaves as a superoxide reductase enzyme and thus provide new insights into the biological mechanisms designed for protection from oxidative stresses