Transdisciplinary participatory-action-research from questions to actionable knowledge for sustainable viticulture development

Viticulture negatively impacts the environment, biodiversity, and human health; however, despite the widely acknowledged challenges that this intensive agricultural activity poses to sustainable development, measures to reduce its invasiveness are constantly being deferred or rebuffed. Constraints to change are linked to vine cultivation methods, the impacts of climate change on vine resilience and disease sensitivity, and socio-economic models, as well as growing criticisms from society. Research and training have thus far failed to provide solutions or mobilise stakeholders on a large scale. Such resistance to sustainable practices development calls into question the effectiveness of knowledge production systems and relations between scientists, winegrowers, and society: Have scientific disciplines overly isolated themselves from each other and from the wider society to the point of losing the capacity to incorporate alternative forms of knowledge and reasoning and achieve collaborative action? Herein, we describe our findings from a participatory action research project that began in Westhalten, France, in 2013 and ultimately spread to Switzerland and Germany over the next 6 years. We show that participatory action research can mobilise long-term collaborations between winegrowers, NGOs, advisers, elected officials, members of civil society, and researchers, despite differing visions of viticulture and the environment. The epistemological framework of this research promotes consensus-building by valuing complexity and dissensus in knowledge and reasoning such that all actors are involved in experimentation and the production of results. From these findings, consensus statements were collectively elaborated in qualitative and quantitative registers. Once acknowledged by the scientific community, these consensus statements became shareable knowledge. We propose that this renewed interdisciplinarity associating the human and social sciences with agronomic and biological sciences in collaboration with stakeholders produces actionable knowledge that mobilises and engages winegrowers to conceive and implement sustainable viticulture on a transnational scale

Remediation of sediment and water contaminated by copper in small-scaled constructed wetlands: effect of bioaugmentation and phytoextraction

International audienceThe use of plants and microorganisms to mitigate sediment contaminated by copper was studied in microcosms that mimic the functioning of a stormwater basin (SWB) connected to vineyard watershed. The impact of phytoremediation and bioaugmentation with siderophore-producing bacteria on the fate of Cu was studied in two contrasted (batch vs. semi-continuous) hydraulic regimes. The fate of copper was characterised following its discharge at the outlet of the microcosms, its pore water concentration in the sediment, the assessment of its bioaccessible fraction in the rhizosphere and the measurement of its content in plant tissues. Physico-chemical (pH, redox potential) and biological parameters (total heterotrophic bacteria) were also monitored. As expected, the results showed a clear impact of the hydraulic regime on the redox potential and thus on the pore water concentration of Cu. Copper in pore water was also dependent on the frequency of Cu-polluted water discharges. Repeated bioaugmentation increased the total heterotrophic microflora as well as the Cu bioaccessibility in the rhizosphere and increased the amount of Cu extracted by Phragmites australis by a factor of ~2. Sugar beet pulp, used as a filter to avoid copper flushing, retained 20%of outcoming Cu and led to an overall retention of Cu higher than 94 % when arranged at the outlet of microcosms. Bioaugmentation clearly improved the phytoextraction rate of Cu in a small-scaled SWB designed to mimic the functioning of a full-size SWB connected to vineyard watershed

Bioremediation of copper-contaminated soils by bacteria

Although copper (Cu) is an essential micronutrient for all living organisms, it can be toxic at low concentrations. Its beneficial effects are therefore only observed for a narrow range of concentrations. Anthropogenic activities such as fungicide spraying and mining have resulted in the Cu contamination of environmental compartments (soil, water and sediment) at levels sometimes exceeding the toxicity threshold. This review focuses on the bioremediation of copper-contaminated soils. The mechanisms by which microorganisms, and in particular bacteria, can mobilize or immobilize Cu in soils are described and the corresponding bioremediation strategies—of varying levels of maturity—are addressed: (i) bioleaching as a process for the ex situ recovery of Cu from Cu-bearing solids, (ii) bioimmobilization to limit the in situ leaching of Cu into groundwater and (iii) bioaugmentation-assisted phytoextraction as an innovative process for in situ enhancement of Cu removal from soil. For each application, the specific conditions required to achieve the desired effect and the practical methods for control of the microbial processes were specified

As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe.

The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.journal articleresearch support, non-u.s. gov't1995 Julimporte

Responses to climatic and pathogen threats differ in biodynamic and conventional vines

Viticulture is of high socio-economic importance; however, its prevalent practices severely impact the environment and human health, and criticisms from society are raising. Vine managements systems are further challenged by climatic changes. Of the 8 million hectares grown worldwide, conventional and organic practices cover 90% and 9% of acreage, respectively. Biodynamic cultivation accounts for 1%. Although economic success combined with low environmental impact is widely claimed by biodynamic winegrowers from California, to South Africa, and France, this practice is still controversial in viticulture and scientific communities. To rethink the situation, we encouraged stakeholders to confront conventional and biodynamic paradigms in a Participative-Action-Research. Co-designed questions were followed up by holistic comparison of conventional and biodynamic vineyard managements. Here we show that the amplitude of plant responses to climatic threats was higher in biodynamic than conventional management. The same stood true for seasonal trends and pathogens attacks. This was associated with higher expression of silencing and immunity genes, and higher anti-oxidative and antifungal secondary metabolite levels. This suggests that sustainability of biodynamic practices probably relies on fine molecular regulations. Such knowledge should contribute to resolving disagreements between stakeholders and help designing the awaited sustainable viticulture at large