6,235 research outputs found
Genome-wide tests for introgression between cactophilic Drosophila implicate a role of inversions during speciation
K.L. was funded by a junior research fellowship from the National Environmental Research Council, UK (NE/I020288/1, NBAF659).Models of speciation-with-gene-flow have shown that the reduction in recombination between alternative chromosome arrangements can facilitate the fixation of locally adaptive genes in the face of gene flow and contribute to speciation. However, it has proven frustratingly difficult to show empirically that inversions have reduced gene flow and arose during or shortly after the onset of species divergence rather than represent ancestral polymorphisms. Here, we present an analysis of whole genome data from a pair of cactophilic fruit flies, Drosophila mojavensis and D. arizonae, which are reproductively isolated in the wild and differ by several large inversions on three chromosomes. We found an increase in divergence at rearranged compared to colinear chromosomes. Using the density of divergent sites in short sequence blocks we fit a series of explicit models of species divergence in which gene flow is restricted to an initial period after divergence and may differ between colinear and rearranged parts of the genome. These analyses show that D. mojavensis and D. arizonae have experienced postdivergence gene flow that ceased around 270 KY ago and was significantly reduced in chromosomes with fixed inversions. Moreover, we show that these inversions most likely originated around the time of species divergence which is compatible with theoretical models that posit a role of inversions in speciation with gene flow.Publisher PDFPeer reviewe
Non-Oberbeck-Boussinesq effects in two-dimensional Rayleigh-Benard convection in glycerol
We numerically analyze Non-Oberbeck-Boussinesq (NOB) effects in
two-dimensional Rayleigh-Benard flow in glycerol, which shows a dramatic change
in the viscosity with temperature. The results are presented both as functions
of the Rayleigh number (Ra) up to (for fixed temperature difference
between the top and bottom plates) and as functions of
"non-Oberbeck-Boussinesqness'' or "NOBness'' () up to 50 K (for fixed
Ra). For this large NOBness the center temperature is more than 5 K
larger than the arithmetic mean temperature between top and bottom plate
and only weakly depends on Ra. To physically account for the NOB deviations of
the Nusselt numbers from its Oberbeck-Boussinesq values, we apply the
decomposition of into the product of two effects, namely
first the change in the sum of the top and bottom thermal BL thicknesses, and
second the shift of the center temperature as compared to . While
for water the origin of the deviation is totally dominated by the second
effect (cf. Ahlers et al., J. Fluid Mech. 569, pp. 409 (2006)) for glycerol the
first effect is dominating, in spite of the large increase of as compared
to .Comment: 6 pages, 7 figure
Response maxima in time-modulated turbulence: Direct Numerical Simulations
The response of turbulent flow to time-modulated forcing is studied by direct
numerical simulations of the Navier-Stokes equations. The large-scale forcing
is modulated via periodic energy input variations at frequency . The
response is maximal for frequencies in the range of the inverse of the large
eddy turnover time, confirming the mean-field predictions of von der Heydt,
Grossmann and Lohse (Phys. Rev. E 67, 046308 (2003)). In accordance with the
theory the response maximum shows only a small dependence on the Reynolds
number and is also quite insensitive to the particular flow-quantity that is
monitored, e.g., kinetic energy, dissipation-rate, or Taylor-Reynolds number.
At sufficiently high frequencies the amplitude of the kinetic energy response
decreases as . For frequencies beyond the range of maximal response,
a significant change in phase-shift relative to the time-modulated forcing is
observed.Comment: submitted to Europhysics Letters (EPL), 8 pages, 8 Postscript
figures, uses epl.cl
Periodically driven Taylor-Couette turbulence
We study periodically driven Taylor-Couette turbulence, i.e. the flow
confined between two concentric, independently rotating cylinders. Here, the
inner cylinder is driven sinusoidally while the outer cylinder is kept at rest
(time-averaged Reynolds number is ). Using particle image
velocimetry (PIV), we measure the velocity over a wide range of modulation
periods, corresponding to a change in Womersley number in the range . To understand how the flow responds to a given modulation, we
calculate the phase delay and amplitude response of the azimuthal velocity.
In agreement with earlier theoretical and numerical work, we find that for
large modulation periods the system follows the given modulation of the
driving, i.e. the system behaves quasi-stationary. For smaller modulation
periods, the flow cannot follow the modulation, and the flow velocity responds
with a phase delay and a smaller amplitude response to the given modulation. If
we compare our results with numerical and theoretical results for the laminar
case, we find that the scalings of the phase delay and the amplitude response
are similar. However, the local response in the bulk of the flow is independent
of the distance to the modulated boundary. Apparently, the turbulent mixing is
strong enough to prevent the flow from having radius-dependent responses to the
given modulation.Comment: 12 pages, 6 figure
Clinical and functional characterisation of a novel TNFRSF1A c.605T > A/V173D cleavage site mutation associated with tumour necrosis factor receptor-associated periodic fever syndrome (TRAPS), cardiovascular complications and excellent response to etanercept treatment.
Objectives: To study the clinical outcome, treatment
response, T-cell subsets and functional consequences of a
novel tumour necrosis factor (TNF) receptor type 1
(TNFRSF1A) mutation affecting the receptor
cleavage site.
Methods: Patients with symptoms suggestive of tumour
necrosis factor receptor-associated periodic syndrome
(TRAPS) and 22 healthy controls (HC) were screened for
mutations in the TNFRSF1A gene. Soluble TNFRSF1A and
inflammatory cytokines were measured by ELISAs.
TNFRSF1A shedding was examined by stimulation of
peripheral blood mononuclear cells (PBMCs) with phorbol
12-myristate 13-acetate followed by flow cytometric
analysis (FACS). Apoptosis of PBMCs was studied by
stimulation with TNFa in the presence of cycloheximide
and annexin V staining. T cell phenotypes were monitored
by FACS.
Results: TNFRSF1A sequencing disclosed a novel V173D/
p.Val202Asp substitution encoded by exon 6 in one
family, the c.194–14G.A splice variant in another and
the R92Q/p.Arg121Gln substitution in two families.
Cardiovascular complications (lethal heart attack and
peripheral arterial thrombosis) developed in two V173D
patients. Subsequent etanercept treatment of the V173D
carriers was highly effective over an 18-month follow-up
period. Serum TNFRSF1A levels did not differ between
TRAPS patients and HC, while TNFRSF1A cleavage from
monocytes was significantly reduced in V173D and R92Q
patients. TNFa-induced apoptosis of PBMCs and T-cell
senescence were comparable between V173D patients
and HC.
Conclusions: The TNFRSF1A V173D cleavage site
mutation may be associated with an increased risk for
cardiovascular complications and shows a strong
response to etanercept. T-cell senescence does not seem
to have a pathogenetic role in affected patients
Concurrent TNFRSF1A R92Q and pyrin E230K mutations in a child with multiple sclerosis
We report a 16-year-old female patient with a severe course of multiple sclerosis and concomitant symptoms suggestive of a hereditary autoinflammatory disease. Genetic analyses revealed that she inherited a TNFRSF1A R92Q mutation from her mother and a pyrin E230K mutation from her father. To our knowledge, this is the first report of a patient with severe childhood multiple sclerosis and mutations in two genes which predispose to hereditary autoinflammatory disorders. We speculate that these mutations contribute to early multiple sclerosis manifestation and enhance the inflammatory damage inflicted by the autoimmune response
Hysteretic clustering in granular gas
Granular material is vibro-fluidized in N=2 and N=3 connected compartments,
respectively. For sufficiently strong shaking the granular gas is
equi-partitioned, but if the shaking intensity is lowered, the gas clusters in
one compartment. The phase transition towards the clustered state is of 2nd
order for N=2 and of 1st order for N=3. In particular, the latter is
hysteretic. The experimental findings are accounted for within a dynamical
model that exactly has the above properties
Archeological Survey Investigations at Martin Creek Lake State Park, Rusk County, Texas
An intensive cultural resources survey was conducted at Martin Creek Lake State Park by the Center for Archaeological Studies and Archeological & Environmental Consultants, LLC in June 2010 under contract with Texas Parks and Wildlife Department. Although previous investigations had been carried out in the park, no comprehensive survey had been completed that would allow TPWD and park management personnel to appropriately manage the park’s cultural resources in accordance with state law. During the 2010 survey, the park was stratified into areas with high, medium, and low probability of containing archaeological sites. A total of 423 shovel tests were excavated in these areas; four new sites were documented and nine previously reported sites were revisited and assessed. These 13 sites reflect historic era occupations in the park dating from the mid-1800s to the mid-1900s and prehistoric occupations that include Late Archaic, Woodland, and Caddo components
Meson exchange model for pseudoscalar meson-meson scattering
A dynamical model for pseudoscalar-pseudoscalar meson scattering based on meson exchange, suitable for use in a variety of low- and intermediate-energy mesonic interactions, has been constructed and applied to ππ and kπ scattering with good quantitative results. The model includes both s- and t-channel exchange, and is found to require pseudoscalar-pseudoscalar coupling to a scalar octet to fit the high energy s-wave phases in the I = 0 ππ channel and in the channel. Coupling of the ππ and KK̄ channels is found to play a crucial role in explaining the S∗(975) resonanc
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