Empowerment or impoverishment of children from social networks? Perceptions of sexualized images of girls in Instagram

Aunque el fenómeno de la sexualización infantil ha motivado numerosas investigaciones durante los últimos años, muy pocas han estudiado cómo lo perciben los adultos, y ninguna lo ha estudiado a partir de las impresiones que provocan las fotografías de niñas sexualizadas en las redes sociales. A partir de los resultados de una encuesta estructurada en internet a 353 personas, este trabajo demuestra que el aspecto sexualizado de las niñas en las redes sociales genera percepciones que las minusvaloran intelectual, social y moralmente. Dichas percepciones dependen del consumo de medios, del grado de auto-cosificación y de la religiosidad de las personas. AbstractAlthough the phenomenon of the sexualization of children has prompted numerous investigations in recent years, very few have studied how adults perceive it, and none has studied the sexualization of girls in photographs in social networks. This work demonstrates, from the results of a structured online survey of 353 people, that the sexualization of girls in social networks generates perceptions that undervalue girls in intellectual, social, and moral aspects. Such perceptions depend on media consumption level, degree of self-objectification, and people’s religiosity

Sexualization in digital advertising of fashion brands for kids: initiatives and ways to report the complaints

Los niños son presentados como objetos sexuales en gran parte, según el debate social, debido a la actividad de marcas y medios vinculados a la moda. La metodología llevada a cabo combina un análisis de contenido de los catálogos de publicidad de moda infantil en Internet en el contexto español, con el objetivo de ver si las marcas de moda infantil sexualizan a los niños en Internet; una revisión de las iniciativas de denuncia en Internet para conocer si la sociedad civil está concienciada; y una revisión de la normativa jurídica y ética en la publicidad digital para verificar si hay una protección a los niños. Los hallazgos apuntan que casi la mitad de los niños de los catálogos (el 45.8%) muestran atributos que los sexualizan, que las niñas son las más perjudicadas -un 48.9% frente al 38.2% de los niños- y las marcas que sexualizan más son las originalmente de adulto. Además, la sexualización se atribuye a la libertad creativa y al beneficio de las marcas. Las iniciativas y quejas se concentran en padres, Gobierno y asociaciones de consumidores. Se recomienda mayor información para que los ciudadanos inicien los procesos de protección legal y ética previstos para proteger a los niños.Children are represented as sexual objects, according to the social debate, mainly due to the activity of brands and media linked to fashion. The methodology conducted has combined a content analysis of the advertising catalogues of fashion kids on Internet in Spanish context to aim the objective of checking if fashion brands for kids are sexualizing them through its advertising on Internet; a review of the initiatives and complaints to report if the civil society is aware; thirdly, a review of the regulation – ethic and legal- on digital advertising to verify if there is a special protection. The results show that almost half of the children on the catalogues (45.8%) displayed sexualizing attributes, that girls are the most damaged -48.9%, while boys reach 38.2%- and that brands derived from those targeted to adults sexualize the most. Therefore, it is highlighted that sexualization is linked to freedom of creativity and brands’ benefits. Initiatives and complaints are focused on parents, Government and consumer associations. The recommendations point to better information to citizens so they can impulse the legal and ethical processes planned to protect infancy

A sexualização infantil na Internet

Childhood are increasingly present in certain digital media, where commercial messages directly appeal to children’s participation, enabling them to express their identity and establishing relations with their peers, who become opinion leaders. The aim of this exploratory qualitative research is to analyse a child’s perception towards sexualized girls depicted in fashion advertising in a digital environment. The investigation conducts a qualitative methodology among Spanish girls between 8 and 11 years old corresponding to the target audience of fashion brands advertising that has been categorized in different levels as sexualizing. The results point out that girls generally reject the images of their peers and models whenever they are portrayed more sexualized because the style does not correspond to real life and does not seem chosen by the girls depicted. Furthermore, girls associate the combination of sexualizing contexts, postures and gestures with personality traits negatively considered -self-centred, defiant, rebel, sad, alone-and are afraid of the normalization among children of make-up and certain behaviours not keeping with their age. In light of the results, the study recommends an ethical reflection by advertisers that use an adult style transgressive and the study of images that depict boys.Los niños están presentes cada vez más en determinados medios digitales, donde los mensajes comerciales apelan directamente a su participación, facilitando expresar su identidad y establecer relaciones con sus iguales, que se convierten en líderes de opinión. El objetivo de este estudio exploratorio inicial y pionero en el formato digital en España es conocer la percepción que tienen las niñas sobre otras niñas sexualizadas en la publicidad de moda y en Internet, y los valores que asocian a ellas. La metodología empleada ha sido un estudio exploratorio cualitativo a una muestra de niñas españolas entre 8 y 11 años que corresponde al público objetivo de marcas de moda cuya publicidad ha sido categorizada en distintos grados como sexualizante. Los resultados señalan que las niñas rechazan aquellas imágenes de las modelos cuando son representadas de una forma más sexualizada porque no se corresponde con la vida real y no parece un estilo elegido sino impuesto. Además, las entrevistadas asocian la combinación de escenarios, posturas y gestos sexualizantes con rasgos de la personalidad que entienden como negativos –egoísta, desafiante, rebelde, aislada, triste-y, exponen el temor de que se normalice el uso de un tipo de ropa, maquillaje y comportamientos no acordes con su edad. A la luz de los resultados, se recomienda la reflexión ética de publicitarios que usan el estilo transgresor adulto en moda infantil y el estudio de imágenes de niños.As crianças estão presentes cada vez mais em algum meios digitais, onde as mensagens comerciais apelam diretamente à sua participação, permitindo-lhes expressar a sua identidade e estabelecer relações com os seus pares, que se tornam líderes de opinião. O objetivo deste estudo exploratório inicial, pioneiro no formato digital em Espanha, é analisar a perceção que as raparigas têm de outras raparigas sexualizadas na publicidade de moda e na Internet, e os valores que lhes associam. A metodologia utilizada foi um estudo exploratório qualitativo de uma amostra de raparigas espanholas entre os 8 e os 11 anos de idade, correspondente ao público-alvo das marcas de moda cuja publicidade foi classificada, em diferentes graus, como sexualizada. Os resultados indicam que as raparigas rejeitam essas imagens de modelos quando são retratadas de uma forma mais sexualizada porque não corresponde à vida real e não parece ser um estilo escolhido, mas sim um estilo imposto. Além disso, associam a combinação de cenários, posturas e gestos sexuais a traços de personalidade que entendem como negativos - egoístas, desafiadores, rebeldes, isolados, tristes - e, expõem o medo da normalização do uso de um tipo de roupa, maquilhagem e comportamentos não condizentes com a sua idade. À luz dos resultados, recomenda-se a reflexão ética dos anunciantes que utilizam o estilo adulto transgressivo na moda infantil e o estudo das imagens das crianças

Empowerment or impoverishment of children from social networks? Perceptions of sexualized images of girls in Instagram

Although the phenomenon of the sexualization of children has prompted numerous investigations in recent years, very few have studied how adults perceive it, and none has studied the sexualization of girls in photographs in social networks. This work demonstrates, from the results of a structured online survey of 353 people, that the sexualization of girls in social networks generates perceptions that undervalue girls in intellectual, social, and moral aspects. Such perceptions depend on media consumption level, degree of self-objectification, and people’s religiosity

Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays

Background Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols in routine diagnosis. To date, there are only two commercially available Real-Time PCR assays for the routine laboratory detection of T. cruzi DNA in clinical samples: TCRUZIDNA.CE (Diagnostic Bioprobes Srl) and RealCycler CHAG (Progenie Molecular). Our aim was to evaluate the RealCycler CHAG assay taking into account the whole process. Methodology/Principal findings We assessed the usefulness of an automated DNA extraction system based on magnetic particles (EZ1 Virus Mini Kit v2.0, Qiagen) combined with a commercially available Real-Time PCR assay targeting satellite DNA (SatDNA) of T. cruzi (RealCycler CHAG), a methodology used for routine diagnosis in our hospital. It was compared with a well-known strategy combining a commercial DNA isolation kit based on silica columns (High Pure PCR Template Preparation Kit, Roche Diagnostics) with an in-house Real-Time PCR targeting SatDNA. The results of the two methodologies were in almost perfect agreement, indicating they can be used interchangeably. However, when variations in protocol factors were applied (sample treatment, extraction method and Real-Time PCR), the results were less convincing. A comprehensive fine-tuning of the whole procedure is the key to successful results. Guanidine EDTA-blood (GEB) samples are not suitable for DNA extraction based on magnetic particles due to inhibition, at least when samples are not processed immediately. Conclusions/Significance This is the first study to evaluate the RealCycler CHAG assay taking into account the overall process, including three variables (sample treatment, extraction method and Real-Time PCR). Our findings may contribute to the harmonization of protocols between laboratories and to a wider application of Real-Time PCR in molecular diagnostic laboratories associated with health centers.Fil: Abras, Alba. Universidad de Barcelona; España. Universidad de Girona; España. Instituto de Salud Global de Barcelona; EspañaFil: Ballart, Cristina. Universidad de Barcelona; España. Instituto de Salud Global de Barcelona; EspañaFil: Llovet, Teresa. Universitat Autònoma de Barcelona; España. Hospital de la Santa Creu I Sant Pau; EspañaFil: Roig, Carme. Hospital de la Santa Creu I Sant Pau; EspañaFil: Gutiérrez, Cristina. Hospital de la Santa Creu I Sant Pau; EspañaFil: Tebar, Silvia. Universidad de Barcelona; España. Instituto de Salud Global de Barcelona; EspañaFil: Berenguer, Pere. Hospital de la Santa Creu I Sant Pau; EspañaFil: Pinazo, María-Jesús. Instituto de Salud Global de Barcelona; EspañaFil: Posada, Elizabeth. Instituto de Salud Global de Barcelona; EspañaFil: Gascón, Joaquim. Instituto de Salud Global de Barcelona; EspañaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gállego, Montserrat. Instituto de Salud Global de Barcelona; España. Universidad de Barcelona; EspañaFil: Muñoz, Carmen. Hospital de la Santa Creu I Sant Pau; España. Universitat Autònoma de Barcelona; Españ

Taxonomic identification of different species of the genus aeromonas by whole-genome sequencing and use of their species-specific Β-lactamases as phylogenetic markers

Altres ajuts: Departament de Microbobiologia de l'Hospital de la Santa Creu i Sant Pau, i l'Institut de Recerca de l'Hospital de la Santa Creu i Sant PauSome Aeromonas species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal Β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific Β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 Aeromonas genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an A. hydrophila isolate as A. dhakensis. Three (B, C and D) of the four Ambler classes of Β-lactamase genes were found in A. sobria, A. allosacharophila, A. hydrophila and A. dhakensis (bla, bla and bla). A. veronii only showed class-B- and class-D-like matches (bla and bla), whereas those for A. media, A. rivipollensis and A. caviae were class C and D (bla, bla and bla). The phylogenetic tree derived from concatenated sequences of Β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify Aeromonas at the species level, which led to the unexpected identification of A. dhakensis and A.rivipollensis and revealed the resistome of each isolate

Estado de la cuestión sobre la sexualización infantil en el entorno digital y propuestas de alfabetización mediática

Las redes sociales plantean para los menores tanto situaciones de riesgo como respuestas positivas hacia su empoderamiento. El fenómeno social de la sexualización infantil, ya rechazado para las mujeres –por las connotaciones de discriminación, rol pasivo y tolerancia a la violencia–, se ha generalizado en Internet con aparente impunidad por parte de las marcas y medios que reproducen esas imágenes y débil concienciación entre las niñas y los padres que las publican en redes sociales. Ante la llamada de reguladores, padres e investigadores para proteger los derechos de la infancia, los objetivos de esta investigación son conocer el estado de la cuestión sobre la sexualización de la infancia en el entorno digital y recoger algunas propuestas de alfabetización mediática. La metodología utilizada ha sido la revisión de la literatura sobre sexualización, publicidad digital, comercialización de la infancia e infancia y moda en las bases de datos de revistas académicas y bibliotecas universitarias. Las conclusiones arrojan la necesaria contribución de prescriptores de opinión, medios, padres, educadores y anunciantes para evitar reducir la sexualidad a la sexualización y tomar conciencia del impacto de las imágenes comerciales que pueden generar conductas de riesgo

Perceived sexualization in girls' fashion stylings: A Spain-China cross-cultural analysis

Many institutions, social and political groups are warning of the risks associated with the early sexualization of childhood. These agents appeal to the responsibility of the media to avoid creating content that may lead to childhood sexualization and that is easily accessible to all audiences. Responding to this demand and through a cross-cultural Spain-China approach, this work focuses on the analysis of the perception of girls’ sexualization in the fashion stylings disseminated by the media. A survey of 750 Communication and Advertising university students in Spain (N=449) and in China (N=301) was carried out. Five latent sexualization factors identified confirm that perceived sexualization in girls’ fashion styling is a multi-dimensional phenomenon that occurs from the combined use of multiple sexualizing attributes. The country of origin (Spain or China) has been associated with the perception of sexualization and the identified latent sexualizing factors. Finally, an explanatory and highly effective predictive model has been obtained for this type of childhood sexualization in terms of the factors and country of origin. Conclusions suggest that it is necessary to reinforce the training of communication professionals and minors to avoid creating images of sexualized girls through certain styling codes

Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds

Campylobacter jejuni is the causal agent of the food-borne infection with the highest incidence in Europe. Both poultry and wild birds are a major reservoir. To gain insight into the population structure, virulence potential, and antimicrobial resistance (AMR), a collection of 150 isolates from three different ecological niches (broilers, wild birds, and human patients) was studied. Despite the high genetic diversity found, the population structure defined two distinct clusters, one formed mostly by broiler and human isolates and another one by most wild bird isolates. The ST-21 complex exhibits highest prevalence (in humans and broilers), followed by ST-1275 complex (only in wild birds). The ST-48, -45, and -354 complexes were found in all three niches, but represent only 22 out of 150 studied strains. A higher occurrence of AMR and multidrug resistance was detected among broiler and human isolates. Moreover, significant differences were found in the distribution of certain putative virulence genes. Remarkably, many wild bird strains were negative for either cdtA, cdtB, or cdtC from the canonical strain 81-176, whereas all broiler and human strains were positive. These data suggest that the different variants of the cdt genes might be relevant for the efficient colonization of certain hosts by C. jejuni. Our study contributes to the understanding of the role of the diverse Campylobacter reservoirs in the transmission of campylobacteriosis to humans

Serological diagnosis of chronic Chagas disease: Is it time for a change?

Chagas disease has spread to non-endemic areas with human migration. Since no single reference standard test is available, serological diagnosis of chronic Chagas disease requires at least two tests. New generation techniques have significantly improved the accuracy of Chagas disease diagnosis by the use of a large mixture of recombinant antigens with different detection systems, such as chemiluminescence. The aim of the present study was to assess the overall accuracy of a new generation kit, Architect Chagas (cut-off ≥ 1 S/CO, sample relative light units/cut-off value), as a single technique in the diagnosis of chronic Chagas disease. Architect Chagas showed a sensitivity of 100% (95% confidence interval, CI = 99.5-100) and a specificity of 97.6% (95% CI = 95.2-99.9). Five out of six false-positive sera were a consequence of cross-reactivity with Leishmania spp. and all of them achieved results < 5 S/CO. We propose Architect Chagas as a single technique for screening in blood banks and for routine diagnosis in clinical laboratories. Only grey zone and positive sera with a result ≤ 6 S/CO would need to be confirmed by a second serological assay, thus avoiding false-positive sera and the problem of cross-reactivity with Leishmania spp. The application of this proposal would result in important savings in the cost of Chagas disease diagnosis and therefore in the management and control of the disease