Identification of extrachromosomal circular forms of active transposable elements using mobilome-seq

Active transposable elements (TEs) generate insertion polymorphisms that can be detected through genome resequencing strategies. However, these techniques may have limitations for organisms with large genomes or for somatic insertions. Here, we present a method that takes advantage of the extrachromosomal circular DNA (eccDNA) forms of actively transposing TEs in order to detect and characterize active TEs in any plant or animal tissue. Mobilome-seq consists in selectively amplifying and sequencing eccDNAs. It relies on linear digestion of genomic DNA followed by rolling circle amplification of circular DNA. Both active DNA transposons and retrotransposons can be identified using this technique

Control of gas-phase nucleation and flow visualization in a special cold wall CVD reactor

The deposition of the ternary compound Ti-N-Si using the cold-wall CVD technique from TiCl4-NH3-SiH2Cl2 presents difficulties because of undesired powder formation in the gas-phase. For a better understanding and control of the mechanism, both simulation and experiments have been carried out to determine favorable conditions to minimize the undesired adducts. First, an original technique has been developed for the reactive injection with a double-passage nozzle specially designed. Then powders have been deliberately grown at different conditions, with their chemical compositions analyzed using X-ray diffraction spectra. Further, the flow has been modeled and compared with the results provided by means of laser sheet visualization. It is shown that the powders can be avoided with some special reactive injection schemes and by controlling the nozzle exit-substrate distance. Some useful information is also delivered on controlling the color and the composition of powders during their synthesis

Kinetic study of free-radical solution copolymerization of N-acryloylmorpholine with an activated ester-type monomer, N-acryloxy succinimide

International audienc

Investigation of ethylene/butadiene copolymers microstructure by H-1 and C-13 NMR

International audienc

Transpositional landscape of rice genome revealed by Paired-End Mapping of high-throughput resequencing data

Transposable elements (TEs) are mobile entities that densely populate most eukaryotic genomes and contribute to both their structural and functional dynamics. However most TE-related sequences found in both plant and animal genomes correspond to inactive, degenerated elements, due to the combined effect of silencing pathways and elimination through deletions. Therefore, one of the major difficulties in fully characterizing the molecular basis of genetic diversity of a given species is to establish its genome-wide transpositional activity. In this report, we provide an extensive survey of the transpositional landscape of a plant genome using a deep sequencing strategy. This was achieved through Paired-End Mapping (PEM) of a 4x coverage of the genome of rice mutant line derived from in vitro callus culture using the Illumina technology. Our study reveals that at least 13 TE families are active in this genotype, causing 34 new insertions. This NGS-based strategy provides new opportunities to quantify the impact of TEs on the genome dynamics of the species

Flanking sequence tags in Arabidopsis thaliana T-DNA insertion lines : a pilot study

International audienc

Flanking sequence tags in Arabidopsis thaliana T-DNA insertion lines : a pilot study

International audienc