Overexpression of β1 integrin contributes to polarity reversal and a poor prognosis of breast invasive micropapillary carcinoma

© Liu et al. Invasive micropapillary carcinoma (IMPC) of the breast is a highly aggressive breast cancer. Polarity reversal exemplified by cluster growth is hypothesized to contribute to the invasiveness and metastasis of IMPC. In this study, we demonstrate that levels of β1 integrin and Rac1 expression were greater in breast IMPC than in invasive breast carcinoma of no specific type and paraneoplastic benign breast tissue. We show that silencing β1 integrin expression using the β1 integrin inhibitor AIIB2 partially restored polarity in IMPC primary cell clusters and downregulated Rac1. Thus, overexpression of β1 integrin upregulates Rac1. Univariate analysis showed that overexpression of β1 integrin and Rac1 was associated with breast cancer cell polarity reversal, lymph node metastasis, and poor disease-free survival in IMPC patients. Multivariate analysis revealed that polarity reversal was an independent predictor of poor disease-free survival. These findings indicate that overexpression of β1 integrin and the resultant upregulation of Rac1 contribute to polarity reversal and metastasis of breast IMPC, and that β1 integrin and Rac1 could be potential prognostic biomarkers and targets for treatment of breast IMPC

Spatial variability of soil salinity in Bohai Sea coastal wetlands, China: Partition into four management zones

Soil salinization constitutes an environmental hazard worldwide. The Bohai Sea coastal wetland area is experiencing dramatic soil salinization, which is affecting its economic development. This study focused on the spatial variation and distribution characteristics of soil salinity in this area using geostatistical analysis combined with the kriging interpolation method, based on a large-scale field investigation and layered soil sampling (0-30, 30-60 and 60-100cm). The results revealed that soil salinity in these layers demonstrated strong variability, obvious spatial structure characteristics and strong spatial autocorrelation. Soil salinity displayed a significant zonal distribution, gradually decreasing with increasing distance from the coastline. Apart from the northern part of the study area, which appeared to be not affected by soil salinization, there were varying degrees of soil salinization in nearly 70% of the total area. With increasing soil depth, the areas of non-salinized and mild salinized soil gradually decreased, while those of moderate salinized and strong salinized soils increased. The area of saline soil first decreased and then increased. The study area could be divided into four management zones according to soil salinities in the top 1-m soil body, and utilization measures, adapted to local conditions, were proposed for each zone. The results of our study present an important theoretical basis for the improvement of saline soils, for wetland re-vegetation and for the sustainable utilization of soil resources in the Bohai Sea coastal wetland.Soil salinization constitutes an environmental hazard worldwide. The Bohai Sea coastal wetland area is experiencing dramatic soil salinization, which is affecting its economic development. This study focused on the spatial variation and distribution characteristics of soil salinity in this area using geostatistical analysis combined with the kriging interpolation method, based on a large-scale field investigation and layered soil sampling (0-30, 30-60 and 60-100cm). The results revealed that soil salinity in these layers demonstrated strong variability, obvious spatial structure characteristics and strong spatial autocorrelation. Soil salinity displayed a significant zonal distribution, gradually decreasing with increasing distance from the coastline. Apart from the northern part of the study area, which appeared to be not affected by soil salinization, there were varying degrees of soil salinization in nearly 70% of the total area. With increasing soil depth, the areas of non-salinized and mild salinized soil gradually decreased, while those of moderate salinized and strong salinized soils increased. The area of saline soil first decreased and then increased. The study area could be divided into four management zones according to soil salinities in the top 1-m soil body, and utilization measures, adapted to local conditions, were proposed for each zone. The results of our study present an important theoretical basis for the improvement of saline soils, for wetland re-vegetation and for the sustainable utilization of soil resources in the Bohai Sea coastal wetland

Impact of the Kuroshio intrusion on the nutrient inventory in the upper northern South China Sea: insights from an isopycnal mixing model

Based on four cruises covering a seasonal cycle in 2009-2011, we examined the impact of the Kuroshio intrusion, featured by extremely oligotrophic waters, on the nutrient inventory in the central northern South China Sea (NSCS). The nutrient inventory in the upper 100m of the water column in the study area ranged from similar to 200 to similar to 290 mmol m(-2) for N+N (nitrate plus nitrite), from similar to 13 to similar to 24 mmol m(-2) for soluble reactive phosphate and from similar to 210 to similar to 430 mmol m(-2) for silicic acid. The nutrient inventory showed a clear seasonal pattern with the highest value appearing in summer, while the N+N inventory in spring and winter had a reduction of similar to 13 and similar to 30 %, respectively, relative to that in summer. To quantify the extent of the Kuroshio intrusion, an isopycnal mixing model was adopted to derive the proportional contribution of water masses from the SCS proper and the Kuroshio along individual isopycnal surfaces. The derived mixing ratio along the isopycnal plane was then employed to predict the genuine gradients of nutrients under the assumption of no biogeochemical alteration. These predicted nutrient concentrations, denoted as N-m, are solely determined by water mass mixing. Results showed that the nutrient inventory in the upper 100m of the NSCS was overall negatively correlated to the Kuroshio water fraction, suggesting that the Kuroshio intrusion significantly influenced the nutrient distribution in the SCS and its seasonal variation. The difference between the observed nutrient concentrations and their corresponding Nm allowed us to further quantify the nutrient removal/addition associated with the biogeochemical processes on top of the water mass mixing. We revealed that the nutrients in the upper 100m of the water column had a net consumption in both winter and spring but a net addition in fall.Based on four cruises covering a seasonal cycle in 2009-2011, we examined the impact of the Kuroshio intrusion, featured by extremely oligotrophic waters, on the nutrient inventory in the central northern South China Sea (NSCS). The nutrient inventory in the upper 100m of the water column in the study area ranged from similar to 200 to similar to 290 mmol m(-2) for N+N (nitrate plus nitrite), from similar to 13 to similar to 24 mmol m(-2) for soluble reactive phosphate and from similar to 210 to similar to 430 mmol m(-2) for silicic acid. The nutrient inventory showed a clear seasonal pattern with the highest value appearing in summer, while the N+N inventory in spring and winter had a reduction of similar to 13 and similar to 30 %, respectively, relative to that in summer. To quantify the extent of the Kuroshio intrusion, an isopycnal mixing model was adopted to derive the proportional contribution of water masses from the SCS proper and the Kuroshio along individual isopycnal surfaces. The derived mixing ratio along the isopycnal plane was then employed to predict the genuine gradients of nutrients under the assumption of no biogeochemical alteration. These predicted nutrient concentrations, denoted as N-m, are solely determined by water mass mixing. Results showed that the nutrient inventory in the upper 100m of the NSCS was overall negatively correlated to the Kuroshio water fraction, suggesting that the Kuroshio intrusion significantly influenced the nutrient distribution in the SCS and its seasonal variation. The difference between the observed nutrient concentrations and their corresponding Nm allowed us to further quantify the nutrient removal/addition associated with the biogeochemical processes on top of the water mass mixing. We revealed that the nutrients in the upper 100m of the water column had a net consumption in both winter and spring but a net addition in fall

New progress in LURR-integrating with the dimensional method

The evolution laws of LURR (Loading-Unloading Response Ratio) before strong earthquakes, especially the peak point of LURR, are described in this paper. The results of four methods (experimental, numerical simulation, seismic data analysis and with damage mechanics analysis) lead to a consistent conclusion-the evolution laws of LURR before strong earthquakes are that, at the early stage of the seismic cycle, LURR will fluctuate around 1 and in the late stage, it rises swiftly and to its peak point. At some time after this peak point, a catastrophic event or events occur. These do not occur at the peak point, but lag behind. The lag time which is denoted by T (2) depends on the magnitude M of the upcoming earthquake among other factors. In order to consider the influence of geophysical parameters in a specific region such as E (a) and J ((t)), where is the shear strain rate of tectonic loading in situ, E (a) is the sum of radiated energy of all earthquake occurring in a specific region measured during a long time duration (110 years in this paper) divided by the area of the region and the time duration, and J ((t)) is a parameter denoting the LURR anomaly area weighted with Y (the value of LURR) and represents the expanse and degree of the seismogenic zone. The dimensional analysis method has been used to reveal the relation between M, T (2) and other parameters in situ for more reliable earthquake prediction

Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis

Vulnerability of Eco-Hydrological Environment in the Yellow River Delta Wetland

We investigated the relationship between groundwater head and oceanic tidal fluctuations in the Yellow River Delta wetland through on-site hydrological monitoring. Shallow groundwater heads were obviously affected by oceanic tide along the coastal zone. The ranges of the wetland zone can be readily assessed by measuring fluctuation amplitudes or lags. The results show that the influence radius is approximately 12 km to 18 km (when the correlation coefficient is 0.7 to 0.8) under the joint actions of oceanic tide and shallow groundwater seepage flow in clayey silt coastal wetland. A cross-sectional sketch of the coastal wetland model is developed based on monitoring data of groundwater and oceanic tidal fluctuations to study the vulnerability of the eco-hydrological environment in the Yellow River Delta wetland. The coastal wetland consists of three zones (the groundwater seepage zone, the tidal-induced transitional zone, and the tidal zone) with distinctly different hydraulic properties. Analytical solutions are used to estimate the vulnerability of the ecohydrological environment in the wetland aquifer located in the NE part of the Yellow River Delta wetland, Shandong Province, China. Our results show that changes in the shallow groundwater quality of the wetland are significantly affected by natural factors, such as strong cutoff in the lower reaches, storm tides, and human engineering activities. The northern coastal wetland may be submerged without damp proof when the height of a storm tide reaches 2.4 m. The depth of shallow groundwater and the salinity gradient are key factors that contribute to the vulnerability of the ecological environment. The vulnerability of the eco-hydrological environment is derived from the joint actions of groundwater dynamics, hydrochemistry, and tidal-induced processes under sedimentary stress and water pressure.We investigated the relationship between groundwater head and oceanic tidal fluctuations in the Yellow River Delta wetland through on-site hydrological monitoring. Shallow groundwater heads were obviously affected by oceanic tide along the coastal zone. The ranges of the wetland zone can be readily assessed by measuring fluctuation amplitudes or lags. The results show that the influence radius is approximately 12 km to 18 km (when the correlation coefficient is 0.7 to 0.8) under the joint actions of oceanic tide and shallow groundwater seepage flow in clayey silt coastal wetland. A cross-sectional sketch of the coastal wetland model is developed based on monitoring data of groundwater and oceanic tidal fluctuations to study the vulnerability of the eco-hydrological environment in the Yellow River Delta wetland. The coastal wetland consists of three zones (the groundwater seepage zone, the tidal-induced transitional zone, and the tidal zone) with distinctly different hydraulic properties. Analytical solutions are used to estimate the vulnerability of the ecohydrological environment in the wetland aquifer located in the NE part of the Yellow River Delta wetland, Shandong Province, China. Our results show that changes in the shallow groundwater quality of the wetland are significantly affected by natural factors, such as strong cutoff in the lower reaches, storm tides, and human engineering activities. The northern coastal wetland may be submerged without damp proof when the height of a storm tide reaches 2.4 m. The depth of shallow groundwater and the salinity gradient are key factors that contribute to the vulnerability of the ecological environment. The vulnerability of the eco-hydrological environment is derived from the joint actions of groundwater dynamics, hydrochemistry, and tidal-induced processes under sedimentary stress and water pressure

Honeytubes: Hollow lattice truss reinforced honeycombs for crushing protection

Lattice truss reinforced honeycombs (LTRHs), termed honeytubes, were developed based on a hybrid design of micro-lattice truss and square honeycomb topologies. Carbon fiber reinforced composite and polymer LTRHs were fabricated using different manufacturing approaches. Out-of-plane compression tests were performed on the LTRHs, and the properties were compared with the conventional square honeycombs. The stiffness and strength values of composite LTRHs didn't surpass those of composite square honeycombs due to the manually induced defects. On the other hand, polymeric LTRHs with perfect geometries were stiffer and stronger than the corresponding polymeric square honeycombs. A parametric study of the buckling resistance was carried out via finite element analysis, and the results indicated that hollow lattice stiffens honeycombs and increases the resistance to buckling, while the specific properties of honeytubes depend on their geometrical parameters. Moreover, the crush force efficiency and specific energy absorption were greater than those of square honeycombs and hollow lattice. This work demonstrates that hybrid designs that capitalize on micro-topologies can populate vacant regions in mechanical property charts, and provide increased energy absorption as crushing protection structures. (C) 2016 Elsevier Ltd. All rights reserved

Shear deformation dominates in the soft adhesive layers of the laminated structure of flexible electronics

Flexible electronics has attracted much attention in recent years due to the favorable applications to many emerging devices. A novel laminated structure for a new-generation of flexible electronics is composed of several thin layers where the hard functional components are built glued by soft adhesives. To prevent the premature mechanical failure and to achieve the best performance of the electronics the structural design of such a laminate is of crucial importance. Accordingly it is necessary to establish an analytic model to accurately describe the mechanical behavior of the laminated structure. The available models fall into two categories: those only taking into account the normal strain-induced deformation of the soft adhesive layers and those only incorporating the shear deformation of the same layers. This paper aims to quantitatively figure out which deformation dominates. By establishing an accurate enough analytic model a significant finding is revealed that shear deformation dominates in the soft adhesive layers of the laminated structure of flexible electronics while the normal strain-induced deformation is negligible. The model is Well validated by the finite element method (FEM). The effects of the membrane energy and bending energy of the soft layer are also investigated by incorporating or neglecting the shear energy. The model accurately captures the key quantities such as the strain distribution in each layer and the locations of the neutral mechanical planes of the top and bottom layers. This work is expected to provide the design guidelines for the laminated structure-based flexible electronics. (C) 2016 Elsevier Ltd. All rights reserved.</p

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]