Relativistic quasiparticle time blocking approximation. II. Pygmy dipole resonance in neutron-rich nuclei

Theoretical studies of low-lying dipole strength in even-even spherical nuclei within the relativistic quasiparticle time blocking approximation (RQTBA) are presented. The RQTBA developed recently as an extension of the self-consistent relativistic quasiparticle random phase approximation (RQRPA) enables one to investigate effects of coupling of two-quasiparticle excitations to collective vibrations within a fully consistent calculation scheme based on covariant energy density functional theory. Dipole spectra of even-even $^{130}$Sn -- $^{140}$Sn and $^{68}$Ni -- $^{78}$Ni isotopes calculated within both RQRPA and RQTBA show two well separated collective structures: the higher-lying giant dipole resonance (GDR) and the lower-lying pygmy dipole resonance (PDR) which can be identified by a different behavior of the transition densities of states in these regions.Comment: 28 pages, 13 figure

Low-lying dipole response in the Relativistic Quasiparticle Time Blocking Approximation and its influence on neutron capture cross sections

We have computed dipole strength distributions for nickel and tin isotopes within the Relativistic Quasiparticle Time Blocking approximation (RQTBA). These calculations provide a good description of data, including the neutron-rich tin isotopes $^{130,132}$Sn. The resulting dipole strengths have been implemented in Hauser-Feshbach calculations of astrophysical neutron capture rates relevant for r-process nucleosynthesis studies. The RQTBA calculations show the presence of enhanced dipole strength at energies around the neutron threshold for neutron rich nuclei. The computed neutron capture rates are sensitive to the fine structure of the low lying dipole strength, which emphasizes the importance of a reliable knowledge of this excitation mode.Comment: 15 pages, 4 figures, Accepted in Nucl. Phys.

Discrimination of healthy and cancer cells of the bladder by metabolic state, based on autofluorescence

Bladder cancer is among the most common cancers worldwide (4th in men). It is responsible for high patient morbidity and displays rapid recurrence and progression. Lack of sensitivity of gold standard techniques (white light cystoscopy, voided urine cytology) means many early treatable cases are missed. The result is a large number of advanced cases of bladder cancer which require extensive treatment and monitoring. For this reason, bladder cancer is the single most expensive cancer to treat on a per patient basis. In recent years, autofluorescence spectroscopy has begun to shed light into disease research. Of particular interest in cancer research are the fluorescent metabolic cofactors NADH and FAD. Early in tumour development, cancer cells often undergo a metabolic shift (the Warburg effect) resulting in increased NADH. The ratio of NADH to FAD ("redox ratio") can therefore be used as an indicator of the metabolic status of cells. Redox ratio measurements have been used to differentiate between healthy and cancer breast cells and to monitor cellular responses to therapies. Here, we have demonstrated, using healthy and bladder cancer cell lines, a statistically significant difference in the redox ratio of bladder cancer cells, indicative of a metabolic shift. To do this we customised a standard flow cytometer to excite and record fluorescence specifically from NADH and FAD, along with a method for automatically calculating the redox ratio of individual cells within large populations. These results could inform the design of novel probes and screening systems for the early detection of bladder cancer

SN 2006bp: Probing the Shock Breakout of a Type II-P Supernova

HET optical spectroscopy and unfiltered ROTSE-III photometry spanning the first 11 months since explosion of the Type II-P SN 2006bp are presented. Flux limits from the days before discovery combined with the initial rapid brightening suggest the supernova was first detected just hours after shock breakout. Optical spectra obtained about 2 days after breakout exhibit narrow emission lines corresponding to HeII 4200, HeII 4686, and CIV 5805 in the rest frame, and these features persist in a second observation obtained 5 hours later; however, these emission lines are not detected the following night nor in subsequent observations. We suggest that these lines emanate from material close to the explosion site, possibly in the outer layers of the progenitor that have been ionized by the high energy photons released at shock breakout. A P-Cygni profile is observed around 4450 A in the +2 and +3 day spectra. Previous studies have attributed this feature to high velocity H-beta, but we discuss the possibility that this profile is instead due to HeII 4687. Further HET observations (14 nights in total) covering the spectral evolution across the photometric plateau up to 73 days after breakout and during the nebular phase around day +340 are presented, and expansion velocities are derived for key features. The measured decay slope for the unfiltered light curve is 0.0073 +/- 0.0004 mag/day between days +121 and +335, which is significantly slower than the decay of rate 56Co. We combine our HET measurements with published X-ray, UV, and optical data to obtain a quasi-bolometric light curve through day +60. We see a slow cooling over the first 25 days, but no sign of an early sharp peak; any such feature from the shock breakout must have lasted less than ~1 day.[ABRIDGED]Comment: ApJ accepted, 43 page

A Parameter Study of Type II Supernova Light Curves Using 6 M_odot He Cores

Results of numerical calculations of Type II supernova light curves are presented. The model progenitor stars have 6 $M{_\odot}$ cores and various envelopes, originating from a numerically evolved 20 $M{_\odot}$ star. Five parameters that affect the light curves are examined: the ejected mass, the progenitor radius, the explosion energy, the $^{56}$Ni mass, and the extent of $^{56}$Ni mixing. The following affects have been found: 1) the larger the progenitor radius the brighter the early--time light curve, with little affect on the late--time light curve, 2) the larger the envelope mass the fainter the early light curve and the flatter the slope of the late light curve, 3) the larger the explosion energy the brighter the early light curve and the steeper the slope of the late light curve, 4) the larger the $^{56}$Ni mass the brighter the overall light curve after 20 to 50 days, with no affect on the early light curve, 5) the more extensive the $^{56}$Ni mixing the brighter the early light curve and the steeper the late light curve. The primary parameters affecting the light curve shape are the progenitor radius and the ejected mass. The secondary parameters are the explosion energy, $^{56}$Ni mass and $^{56}$Ni mixing. I find that while in principle the general shape and absolute magnitude of a light curve indicate a unique set of parameters, in practice it is difficult to avoid some ambiguity in the parameters. I find that the nickel--powered diffusion wave and the recombination of helium produce a prominent secondary peak in all our calculations. The feature is less prominent when compositional mixing, both $^{56}$Ni mixing and mixing between the hydrogen and helium layers, occurs. The model photospheric temperatures and velocities are presented, for comparison to observation.Comment: 39 pages, 15 figures. Astrophysical Journal (Accepted, Dec. 20, 2004

Covariant response theory beyond RPA and its application

The covariant particle-vibration coupling model within the time blocking approximation is employed to supplement the Relativistic Random Phase Approximation (RRPA) with coupling to collective vibrations. The Bethe-Salpeter equation in the particle-hole channel with an energy dependent residual particle-hole (p-h) interaction is formulated and solved in the shell-model Dirac basis as well as in the momentum space. The same set of the coupling constants generates the Dirac-Hartree single-particle spectrum, the static part of the residual p-h interaction and the particle-phonon coupling amplitudes. This approach is applied to quantitative description of damping phenomenon in even-even spherical nuclei with closed shells $^{208}$Pb and $^{132}$Sn. Since the phonon coupling enriches the RRPA spectrum with a multitude of ph$\otimes$phonon states a noticeable fragmentation of giant monopole and dipole resonances is obtained in the examined nuclei. The results are compared with experimental data and with results of the non-relativistic approach.Comment: 12 pages, 4 figures, Proceedings of the NSRT06 Conferenc

Isospin Character of the Pygmy Dipole Resonance in 124Sn

The pygmy dipole resonance has been studied in the proton-magic nucleus 124Sn with the (a,a'g) coincidence method at E=136 MeV. The comparison with results of photon-scattering experiments reveals a splitting into two components with different structure: one group of states which is excited in (a,a'g) as well as in (g,g') reactions and a group of states at higher energies which is only excited in (g,g') reactions. Calculations with the self-consistent relativistic quasiparticle time-blocking approximation and the quasiparticle phonon model are in qualitative agreement with the experimental results and predict a low-lying isoscalar component dominated by neutron-skin oscillations and a higher-lying more isovector component on the tail of the giant dipole resonance

Structure of the bacteriophage PhiKZ non-virion RNA polymerase

Bacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. ΦKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a ‘nucleus’, erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. ΦKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. ΦKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial β/β′ subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of ΦKZ nvRNAP to better than 3.0 Å, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to σ-factor

From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index.

Mitochondrial DNA (mtDNA) encodes core subunits of oxidative phosphorylation complexes and, as a result of intricate regulatory crosstalk between nuclear and mitochondrial genomes, the total number of mtDNA copies fits the requirements of each cell type. Deviations from the physiological number of mtDNA copies are expected to be deleterious and might cause some inherited diseases and normal ageing. We studied 46 obese patients with type 2 diabetes (T2DM) one year after a laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB). The results were compared with normal-weight patients without T2DM (control group 1) (body mass index (BMI) = 22.5 ± 3.01 kg/m <sup>2</sup> ) and patients with obesity without T2DM (control group 2) (BMI = 36 ± 3.45 kg/m <sup>2</sup> ). We detected an increase of mtDNA copy number in the cells of the buffy coat obtained from peripheral blood, sampled one year after bariatric surgery. We also found that average mtDNA copy number as well as its dynamics (before and after the surgery) are gender-specific. To the best of our knowledge, this is the first evidence for the restoration of mtDNA copy number in obese patients after LSG and RYGB