Plasma photonic spatiotemporal synchronization of relativistic electron and laser beams

We present an ultracompact plasma-based method to measure spatial and temporal concurrence of intense electron and laser beams nonintrusively at their interaction point. The electron beam couples with a laser-generated seed plasma in dependence of spatiotemporal overlap, which triggers additional plasma production and manifests as enhanced plasma afterglow. This optical observable is exploited to measure beam concurrence with &sim;4&thinsp;&thinsp;&mu;m spatial and &sim;26.7&thinsp;&thinsp;fs temporal accuracy, supported by auxiliary diagnostics. The afterglow interaction fingerprint is highly sensitive and enables ultraversatile femtosecond-micrometer beam metrology.</p

Temporal Evolution of the Light Emitted by a Thin, Laser-ionized Plasma Source

We present an experimental and simulation-based investigation of the temporal evolution of light emission from a thin, laser-ionized Helium plasma source. We demonstrate an analytic model to calculate the approximate scaling of the time-integrated, on-axis light emission with the initial plasma density and temperature, supported by the experiment, which enhances the understanding of plasma light measurement for plasma wakefield accelerator (PWFA) plasma sources. Our model simulates the plasma density and temperature using a split-step Fourier code and a particle-in-cell (PIC) code. A fluid simulation is then used to model the plasma and neutral density, and the electron temperature as a function of time and position. We then show the numerical results of the space-and-time-resolved light emission and that collisional excitation is the dominant source of light emission. We validate our model by measuring the light emitted by a laser-ionized plasma using a novel statistical method capable of resolving the nanosecond-scale temporal dynamics of the plasma light using a cost-effective camera with microsecond-scale timing jitter. This method is ideal for deployment in the high radiation environment of a particle accelerator that precludes the use of expensive nanosecond-gated cameras. Our results show that our models can effectively simulate the dynamics of a thin, laser-ionized plasma source and this work is useful to understand the plasma light measurement, which plays an important role in the PWFA.Comment: 12 pages, 11 figure

Mobile Tele-echography: User Interface Design

Ultrasound imaging allows the evaluation of the degree of emergency of a patient. However, in several occasions a well trained sonographer is unavailable to perform such echography. To cope with this issue, the OTELO project aims to develop a fully integrated end-to-end mobile tele-echography system using an ultra light, remote-controlled robot, for population groups that are not served locally by medical experts. This paper focuses on the user interface of the OTELO system, consisted of the following parts: An ultrasound video transmission system providing real-time images of the scanned area, an audio/video conference to communicate with the paramedical assistant and with the patient, and a virtual reality environment, providing visual and haptic feed-back to the expert, while capturing the expert's hand movements. These movements are reproduced by the robot in the patient site while holding the ultrasound probe against the patient skin. Besides, the user interface includes an image processing facility for enhancing the received images and the possibility to include them into a database

Plasma accelerator-based ultrabright x-ray beams from ultrabright electron beams

We provide a pathway to compact ultrabright light sources, based on ultrabright, high energy electron beams emerging from a combination of plasma Wakefield acceleration and plasma photocathodes. While plasma acceleration is known to produce accelerating fields three or four orders of magnitude larger than conventional accelerators, the plasma photocathode allows production of electron beams three or four orders of magnitude brighter than conventional, and thus is suitable to unleash the full potential of plasma accelerators. In particular, this is the case for various types of light sources, which profit enormously from an increased electron beam brightness. Building on the recent first experimental demonstration of the plasma photocathode, in this work we discuss the prospects of plasma photocathodes for key photon source approaches such as x-ray free-electron lasers, betatron radiation, ion-channel lasers and inverse Compton scattering

Demonstration of a positron beam-driven hollow channel plasma wakefield accelerator

International audiencePlasma wakefield accelerators have been used to accelerate electron and positron particle beams with gradients that are orders of magnitude larger than those achieved in conventional accelerators. In addition to being accelerated by the plasma wakefield, the beam particles also experience strong transverse forces that may disrupt the beam quality. Hollow plasma channels have been proposed as a technique for generating accelerating fields without transverse forces. Here we demonstrate a method for creating an extended hollow plasma channel and measure the wakefields created by an ultrarelativistic positron beam as it propagates through the channel. The plasma channel is created by directing a high-intensity laser pulse with a spatially modulated profile into lithium vapour, which results in an annular region of ionization. A peak decelerating field of 230 MeV/m is inferred from changes in the beam energy spectrum, in good agreement with theory and particle-in-cell simulations

Plasma-photonic spatiotemporal synchronization of relativistic electron and laser beams

Modern particle accelerators and their applications increasingly rely on precisely coordinated interactions of intense charged particle and laser beams. Femtosecond-scale synchronization alongside micrometre-scale spatial precision are essential e.g. for pump-probe experiments, seeding and diagnostics of advanced light sources and for plasma-based accelerators. State-of-the-art temporal or spatial diagnostics typically operate with low-intensity beams to avoid material damage at high intensity. As such, we present a plasma-based approach, which allows measurement of both temporal and spatial overlap of high-intensity beams directly at their interaction point. It exploits amplification of plasma afterglow arising from the passage of an electron beam through a laser-generated plasma filament. The corresponding photon yield carries the spatiotemporal signature of the femtosecond-scale dynamics, yet can be observed as a visible light signal on microsecond-millimetre scales

Rapid movement and transcriptional re-localization of human cohesin on DNA

The spatial organization, correct expression, repair, and segregation of eukaryotic genomes depend on cohesin, ring-shaped protein complexes that are thought to function by entrapping DNA It has been proposed that cohesin is recruited to specific genomic locations from distal loading sites by an unknown mechanism, which depends on transcription, and it has been speculated that cohesin movements along DNA could create three-dimensional genomic organization by loop extrusion. However, whether cohesin can translocate along DNA is unknown. Here, we used single-molecule imaging to show that cohesin can diffuse rapidly on DNA in a manner consistent with topological entrapment and can pass over some DNA-bound proteins and nucleosomes but is constrained in its movement by transcription and DNA-bound CCCTC-binding factor (CTCF). These results indicate that cohesin can be positioned in the genome by moving along DNA, that transcription can provide directionality to these movements, that CTCF functions as a boundary element for moving cohesin, and they are consistent with the hypothesis that cohesin spatially organizes the genome via loop extrusion

A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A

Kinetochores are megadalton-sized protein complexes that mediate chromosome–microtubule interactions in eukaryotes. How kinetochore assembly is triggered specifically on centromeric chromatin is poorly understood. Here we use biochemical reconstitution experiments alongside genetic and structural analysis to delineate the contributions of centromere-associated proteins to kinetochore assembly in yeast. We show that the conserved kinetochore subunits Ame1$^{CENP-U}$ and Okp1$^{CENP-Q}$ form a DNA-binding complex that associates with the microtubule-binding KMN network via a short Mtw1 recruitment motif in the N terminus of Ame1. Point mutations in the Ame1 motif disrupt kinetochore function by preventing KMN assembly on chromatin. Ame1–Okp1 directly associates with the centromere protein C (CENP-C) homologue Mif2 to form a cooperative binding platform for outer kinetochore assembly. Our results indicate that the key assembly steps, CENP-A recognition and outer kinetochore recruitment, are executed through different yeast constitutive centromere-associated network subunits. This two-step mechanism may protect against inappropriate kinetochore assembly similar to rate-limiting nucleation steps used by cytoskeletal polymers

Design and manufacture of a feed and cut machine

Anderson Products currently uses a feed and cut machine to cut bundles of spring-steel wire. These bundles are used to produce a majority of their wire-brush applications. To reach the various forming stages throughout the plant, they must be loaded onto carts and manually transported. While this may work well for applications of lower production speed, it is not efficient for high speed jobs. The objective of this project is to design a fully automated wire feed-and-cut machine that can be integrated with an existing forming station. This requires the machine to operate with less cutting force and occupy less space than the current machine, which uses a 20-ton press