Language, Play, and Toy Sharing in Infancy

Toy sharing between 97 Infants and their mothers was used to measure shared reference, mother and infant attention-directing strategies, and maternal and infant responsiveness. The association of toy sharing with early language and symbolic play was assessed. Infants were videotaped in a 10-minute free-play session at 11 months. Videotapes were coded for frequency of toy exchanges and level of infant symbolic play. Language was assessed at both 11 and 14 months. Maternal responsiveness to infant-initiated toy exchanges was positively related to symbolic play at 11 months. Maternal responsiveness was also related to Productive, Receptive, and Total Language scores at 14 months. Symbolic play at 11 months and language at 11 and 14 months were associated, suggesting underlying cognitive abilities associated with language and play development. Results support the notion that shared reference, maternal responsiveness, and underlying infant cognitive abilities are important components of a context where scaffolding of language and symbolic play can occur

School climate, emotions, and relationships : children’s experiences of well-being in the Midwestern U.S.

The purpose of this phenomenological study was to explore children’s perceptions of school relationships, and the ways in which those relationships supported or undermined children’s emotional well-being (EWB). This sub-study of a multinational comparative investigation of children’s well-being followed a semi-structured qualitative interview protocol. Rural and urban children (age 8 to 13, N = 23) from the Midwestern U.S. completed the interview and mapping exercise used to explore aspects of and influences on their subjective well-being (including school). Phenomenological analyses of interview transcripts focused on 1) the essence of children’s EWB (including emotional valence and arousal) within the context of school relationships and 2) children’s perception of the impact of school relationships on their EWB. A seasonal metaphor captured the essence of children’s experiences of EWB, which naturally clustered into four themes based on emotional intensity and valance: spring, summer, fall, and winter. Children’s emotional experiences with teachers and peers were similarly represented in the themes, with the exception of winter emotions, which diverged. Children expressed complex, multilayered emotions within the school setting that were connected to the quality of school relationships. Findings are discussed in the context of improving school relationships and climate to support children’s EWB.peer-reviewe

Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake.

Copy number (CN) variation (CNV) has been shown to be common in regions of the genome coding for immune-related genes, and thus impacts upon polygenic autoimmunity. Low CN of FCGR3B has recently been associated with systemic lupus erythematosus (SLE). FcgammaRIIIb is a glycosylphosphatidylinositol-linked, low affinity receptor for IgG found predominantly on human neutrophils. We present novel data demonstrating that both in a family with FcgammaRIIIb-deficiency and in the normal population, FCGR3B CNV correlates with protein expression, with neutrophil uptake of and adherence to immune complexes, and with soluble serum FcgammaRIIIb. Reduced FcgammaRIIIb expression is thus likely to contribute to the impaired clearance of immune complexes, which is a feature of SLE, explaining the association between low FCGR3B CNV and SLE that we have confirmed in a Caucasian population. In contrast, antineutrophil cytoplasmic antibody-associated systemic vasculitis (AASV), a disease not associated with immune complex deposition, is associated with high FCGR3B CN. Thus, we define a role for FCGR3B CNV in immune complex clearance, a function that may explain why low FCGR3B CNV is associated with SLE, but not AASV. This is the first report of an association between disease-related gene CNV and variation in protein expression and function that may contribute to autoimmune disease susceptibility

SnoN expression is differently regulated in microsatellite unstable compared with microsatellite stable colorectal cancers

BACKGROUND: SnoN is an important regulator of the transforming growth factor beta (TGFβ) signalling pathway and has been shown to exhibit both tumour promotion and suppression activity. METHODS: To further explore the role of this complex molecule in colorectal tumorigenesis, we examined 52 paired normal and tumour colorectal specimens stratified by level of microsatellite instability; 18 with high-level microsatellite instability (MSI-H) and 34 microsatellite stable (MSS). SnoN transcript expression was quantitated by real-time PCR and analysed with respect to clinical indicators of prognosis. RESULTS: Within the MSI-H subgroup, SnoN was commonly either up-regulated (6/18, 33%) or down-regulated (7/18, 39%). A significantly different distribution of SnoN expression was observed in MSS cancers compared with MSI-H (P ≤ 0.001). Whilst 17/34 (50%) of MSS tumours demonstrated up-regulation, none showed down-regulated expression. Within the MSI-H subgroup, up-regulation was significantly correlated with lack of repeat tract mutation in the TGFβRII gene (P ≤ 0.025), suggesting that SnoN is more frequently up-regulated in the presence of functional TGFβ signalling. CONCLUSION: Together these data support the notion that SnoN has both oncogenic and tumour suppressive properties depending on other genetic changes within the tumour, and that the MSI-H pathway of colorectal tumorigenesis presents an excellent model for the study of these opposing functions

Low-NO atmospheric oxidation pathways in a polluted megacity

The impact of emissions of volatile organic compounds (VOCs) to the atmosphere on the production of secondary pollutants, such as ozone and secondary organic aerosol (SOA), is mediated by the concentration of nitric oxide (NO). Polluted urban atmospheres are typically considered to be “high-NO” environments, while remote regions such as rainforests, with minimal anthropogenic influences, are considered to be “low NO”. However, our observations from central Beijing show that this simplistic separation of regimes is flawed. Despite being in one of the largest megacities in the world, we observe formation of gas- and aerosol-phase oxidation products usually associated with low-NO “rainforest-like” atmospheric oxidation pathways during the afternoon, caused by extreme suppression of NO concentrations at this time. Box model calculations suggest that during the morning high-NO chemistry predominates (95 %) but in the afternoon low-NO chemistry plays a greater role (30 %). Current emissions inventories are applied in the GEOS-Chem model which shows that such models, when run at the regional scale, fail to accurately predict such an extreme diurnal cycle in the NO concentration. With increasing global emphasis on reducing air pollution, it is crucial for the modelling tools used to develop urban air quality policy to be able to accurately represent such extreme diurnal variations in NO to accurately predict the formation of pollutants such as SOA and ozone

Analysis of multichannel internet communication.

A novel method employing machine-based learning to identify messages related to other messages is described and evaluated. This technique may enable an analyst to identify and correlate a small number of related messages from a large sample of individual messages. The classic machine learning techniques of decision trees and naive Bayes classification are seeded with few (or no) messages of interest and 'learn' to identify other related messages. The performance of this approach and these specific learning techniques are evaluated and generalized

Key role of NO3 radicals in the production of isoprene nitrates and nitrooxyorganosulfates in Beijing

The formation of isoprene nitrates (IsN) can lead to significant secondary organic aerosol (SOA) production and they can act as reservoirs of atmospheric nitrogen oxides. In this work, we estimate the rate of production of IsN from the reactions of isoprene with OH and NO3 radicals during the summertime in Beijing. While OH dominates the loss of isoprene during the day, NO3 plays an increasingly important role in the production of IsN from the early afternoon onwards. Unusually low NO concentrations during the afternoon resulted in NO3 mixing ratios of ca. 2 pptv at approximately 15:00, which we estimate to account for around a third of the total IsN production in the gas phase. Heterogeneous uptake of IsN produces nitrooxyorganosulfates (NOS). Two mono-nitrated NOS were correlated with particulate sulfate concentrations and appear to be formed from sequential NO3 and OH oxidation. Di- and tri-nitrated isoprene-related NOS, formed from multiple NO3 oxidation steps, peaked during the night. This work highlights that NO3 chemistry can play a key role in driving biogenic–anthropogenic interactive chemistry in Beijing with respect to the formation of IsN during both the day and night

Language, Social Interactions, and Attention as Predictors of Reading Development in Second Grade

Social interactions between 153 mother-infant dyads in the laboratory were examined for associations with language and play preferences when infants were 14 months old. Later associations with reading skills, attention, and book reading were examined at the end of second grade. Mothers and infants were videotaped in a 20-minute laboratory observation at 14 months, and joint visual attention and social toy play were coded from the interactions. Language was assessed at 14 months using a standardized instrument, and mothers rated their own and their infants\u27 preferences for specific types of play. A follow-up study, conducted at the end of second grade, assessed decoding and reading comprehension skills, attention and distractibility in the classroom and at home, and the frequency of mother-child book reading. A path model was constructed to examine predictive relations from infancy to second grade. The results suggest that early social interactions are both directly and indirectly related to language in infancy. Joint attention was associated with maternal responses during play and infant preferences for point and name games, which were in turn related to language development. Social interactions in infancy were negatively related to cognitive problems in second. There were small bivariate associations between infant language and play interactions with later reading skills. However, the strongest predictors of reading skills in second grade were children\u27s abilities to sustain attention in relation to cognitive tasks in the classroom. The results suggest that early social interactions involving language and play may foster both language abilities and attention-sustaining abilities, which then influence the development of literacy skills