50 research outputs found
Estimating the host genetic contribution to the epidemiology of infectious diseases
Reducing disease prevalence through selection for host resistance offers a desirable
alternative to chemical treatment which is a potential environmental concern due to
run-off, and sometimes only offers limited protection due to pathogen resistance for
example (Chen et al., 2010). Genetic analyses require large sample sizes and hence
disease phenotypes often need to be obtained from field data. Disease data from field
studies is often binary, indicating whether an individual became infected or not
following exposure to infectious pathogens. In genetic analyses of binary disease
data, however, exposure is often considered as an environmental constant and thus
potential variation in host infectivity is ignored. Host infectivity is the propensity of
an infected individual to infect others. The lack of attention to genetic variation in
infectivity stands in contrast to its important role in epidemiology.
The theory of indirect genetic effects (IGE), also known as associative or social
genetic effects, provides a promising framework to account for genetic variation in
infectivity as it investigates heritable effects of an individual on the trait value of
another individual. Chapter 2 examines to what extent genetic variance in
infectivity/susceptibility is captured by a conventional model versus an IGE model.
The results show that, unlike a conventional model, which does not capture the
variation in infectivity when it is present in the data, a model which takes IGEs into
account captures some, though not all, of the inherent genetic variation in infectivity.
The results also show that genetic evaluations that incorporate variation in infectivity
can increase response to selection and reduce future disease risk. However, the
results of this study also reveal severe shortcomings in using the standard IGE model
to estimate genetic variance in infectivity caused by ignoring dynamic aspects of
disease transmission.
Chapter 3 explores to what extent the standard IGE model could be adapted for use
with binary infectious disease data taking account of dynamic properties within the
remit of a conventional quantitative genetics mixed model framework and software.
The effect of including disease dynamics in this way was assessed by comparing the
accuracy, bias and impact for estimates obtained for simulated binary disease data
with two such adjusted IGE models, with the Standard IGE model. In the first
adjusted model, the Case model, it was assumed that only infected individuals have
an indirect effect on their group mates. In the second adjusted IGE model, the Case-ordered
model, it was assumed that only infected individuals exert an indirect effect
on susceptible group mates only. The results show that taking the disease status of
individuals into account, by using the Case model, considerably improves the bias,
accuracy and impact of genetic infectivity estimates from binary disease data
compared to the Standard IGE model. However, although heuristically one would
assume that the Case-ordered model would provide the best estimates, as it takes the
disease dynamics into account, in fact it provides the worst. Moreover, the results
suggest that further improvements would be necessary in order to achieve
sufficiently reliable infectivity estimates, and point to inadequacy of the statistical
model.
In order to derive an appropriate relationship between the observed binary disease
trait and underlying susceptibility and infectivity, epidemiological theory was
combined with quantitative genetics theory to expand the existing framework in
Chapter 4. This involved the derivation of a genetic-epidemiological function which
takes dynamic expression of susceptibility and infectivity into account. When used to
predict the outcome of simulated data it proved to be a good fit for the probability of
an individual to become infected given its own susceptibility and the infectivity of its
group mates. Using the derived function it was demonstrated that the use of a linear
IGE model would result in biased estimates of susceptibility and infectivity as
observed in Chapters 2 & 3.
Following the results of Chapter 4, the derived expression was used to develop a
Markov Chain Monte Carlo (MCMC) algorithm in order to estimate breeding values
in susceptibility and infectivity in Chapter 5. The MCMC algorithm was evaluated
with simulated disease data. Prior to implementing this algorithm with real disease
data an adequate experimental design must be determined. The results suggest that
there is a trade-off for the ability to estimate susceptibility and infectivity with
regards to group size; this is in line with findings for IGE models. A possible
compromise would be to place relatives in both larger and smaller groups. The
general discussion addresses such questions regarding experimental design and
possible areas for improvement of the algorithm.
In conclusion, the thesis advances and develops a novel approach to the analysis of
binary infectious disease data, which makes it possible to capture genetic variation in
both host susceptibility and infectivity. This approach has been refined to make those
estimates increasingly accurate. These breeding values will provide novel
opportunities for genome wide association studies and may lead to novel genetic
disease control strategies tackling not only host resistance but also the ability to
transmit infectious agents
Indirect Genetic Effects and the Spread of Infectious Disease: Are We Capturing the Full Heritable Variation Underlying Disease Prevalence?
Reducing disease prevalence through selection for host resistance offers a desirable alternative to chemical treatment. Selection for host resistance has proven difficult, however, due to low heritability estimates. These low estimates may be caused by a failure to capture all the relevant genetic variance in disease resistance, as genetic analysis currently is not taylored to estimate genetic variation in infectivity. Host infectivity is the propensity of transmitting infection upon contact with a susceptible individual, and can be regarded as an indirect effect to disease status. It may be caused by a combination of physiological and behavioural traits. Though genetic variation in infectivity is difficult to measure directly, Indirect Genetic Effect (IGE) models, also referred to as associative effects or social interaction models, allow the estimation of this variance from more readily available binary disease data (infected/non-infected). We therefore generated binary disease data from simulated populations with known amounts of variation in susceptibility and infectivity to test the adequacy of traditional and IGE models. Our results show that a conventional model fails to capture the genetic variation in infectivity inherent in populations with simulated infectivity. An IGE model, on the other hand, does capture some of the variation in infectivity. Comparison with expected genetic variance suggests that there is scope for further methodological improvement, and that potential responses to selection may be greater than values presented here. Nonetheless, selection using an index of estimated direct and indirect breeding values was shown to have a greater genetic selection differential and reduced future disease risk than traditional selection for resistance only. These findings suggest that if genetic variation in infectivity substantially contributes to disease transmission, then breeding designs which explicitly incorporate IGEs might help reduce disease prevalence
Risk factors and variations in detection of new bovine tuberculosis breakdowns via slaughterhouse surveillance in Great Britain.
Slaughterhouse surveillance through post-mortem meat inspection provides an important mechanism for detecting bovine tuberculosis (bTB) infections in cattle herds in Great Britain (GB), complementary to the live animal skin test based programme. We explore patterns in the numbers of herd breakdowns detected through slaughterhouse surveillance and develop a Bayesian hierarchical regression model to assess the associations of animal-level factors with the odds of an infected animal being detected in the slaughterhouse, allowing us to highlight slaughterhouses that show atypical patterns of detection. The analyses demonstrate that the numbers and proportions of breakdowns detected in slaughterhouses increased in GB over the period of study (1998-2013). The odds of an animal being a slaughterhouse case was strongly associated with the region of the country that the animal spent most of its life, with animals living in high-frequency testing areas of England having on average 21 times the odds of detection compared to animals living in Scotland. There was also a strong effect of age, with animals slaughtered at > 60 months of age having 5.3 times the odds of detection compared to animals slaughtered between 0-18 months of age. Smaller effects were observed for cattle having spent time on farms with a history of bTB, quarter of the year that the animal was slaughtered, movement and test history. Over-and-above these risks, the odds of detection increased by a factor of 1.1 for each year of the study. After adjustment for these variables, there were additional variations in risk between slaughterhouses and breed. Our framework has been adopted into the routine annual surveillance reporting carried out by the Animal Plant Health Agency and may be used to target more detailed investigation of meat inspection practices.Defra Project SE3133. (Department of Environment and Rural Affairs, UK Government
Bias, accuracy, and impact of indirect genetic effects in infectious diseases
Selection for improved host response to infectious disease offers a desirable alternative to chemical treatment but has proven difficult in practice, due to low heritability estimates of disease traits. Disease data from field studies is often binary, indicating whether an individual has become infected or not following exposure to an infectious disease. Numerous studies have shown that from this data one can infer genetic variation in individuals’ underlying susceptibility. In a previous study, we showed that with an indirect genetic effect (IGE) model it is possible to capture some genetic variation in infectivity, if present, as well as in susceptibility. Infectivity is the propensity of transmitting infection upon contact with a susceptible individual. It is an important factor determining the severity of an epidemic. However, there are severe shortcomings with the Standard IGE models as they do not accommodate the dynamic nature of disease data. Here we adjust the Standard IGE model to (1) make expression of infectivity dependent on the individuals’ disease status (Case Model) and (2) to include timing of infection (Case-ordered Model). The models are evaluated by comparing impact of selection, bias, and accuracy of each model using simulated binary disease data. These were generated for populations with known variation in susceptibility and infectivity thus allowing comparisons between estimated and true breeding values. Overall the Case Model provided better estimates for host genetic susceptibility and infectivity compared to the Standard Model in terms of bias, impact, and accuracy. Furthermore, these estimates were strongly influenced by epidemiological characteristics. However, surprisingly, the Case-Ordered model performed considerably worse than the Standard and the Case Models, pointing toward limitations in incorporating disease dynamics into conventional variance component estimation methodology and software used in animal breeding
A unifying theory for genetic epidemiological analysis of binary disease data
BACKGROUND: Genetic selection for host resistance offers a desirable complement to chemical treatment to control infectious disease in livestock. Quantitative genetics disease data frequently originate from field studies and are often binary. However, current methods to analyse binary disease data fail to take infection dynamics into account. Moreover, genetic analyses tend to focus on host susceptibility, ignoring potential variation in infectiousness, i.e. the ability of a host to transmit the infection. This stands in contrast to epidemiological studies, which reveal that variation in infectiousness plays an important role in the progression and severity of epidemics. In this study, we aim at filling this gap by deriving an expression for the probability of becoming infected that incorporates infection dynamics and is an explicit function of both host susceptibility and infectiousness. We then validate this expression according to epidemiological theory and by simulating epidemiological scenarios, and explore implications of integrating this expression into genetic analyses. RESULTS: Our simulations show that the derived expression is valid for a range of stochastic genetic-epidemiological scenarios. In the particular case of variation in susceptibility only, the expression can be incorporated into conventional quantitative genetic analyses using a complementary log-log link function (rather than probit or logit). Similarly, if there is moderate variation in both susceptibility and infectiousness, it is possible to use a logarithmic link function, combined with an indirect genetic effects model. However, in the presence of highly infectious individuals, i.e. super-spreaders, the use of any model that is linear in susceptibility and infectiousness causes biased estimates. Thus, in order to identify super-spreaders, novel analytical methods using our derived expression are required. CONCLUSIONS: We have derived a genetic-epidemiological function for quantitative genetic analyses of binary infectious disease data, which, unlike current approaches, takes infection dynamics into account and allows for variation in host susceptibility and infectiousness
A Novel Statistical Model to Estimate Host Genetic Effects Affecting Disease Transmission
There is increasing recognition that genetic diversity can affect the spread of diseases, potentially affecting plant and livestock disease control as well as the emergence of human disease outbreaks. Nevertheless, even though computational tools can guide the control of infectious diseases, few epidemiological models can simultaneously accommodate the inherent individual heterogeneity in multiple infectious disease traits influencing disease transmission, such as the frequently modeled propensity to become infected and infectivity, which describes the host ability to transmit the infection to susceptible individuals. Furthermore, current quantitative genetic models fail to fully capture the heritable variation in host infectivity, mainly because they cannot accommodate the nonlinear infection dynamics underlying epidemiological data. We present in this article a novel statistical model and an inference method to estimate genetic parameters associated with both host susceptibility and infectivity. Our methodology combines quantitative genetic models of social interactions with stochastic processes to model the random, nonlinear, and dynamic nature of infections and uses adaptive Bayesian computational techniques to estimate the model parameters. Results using simulated epidemic data show that our model can accurately estimate heritabilities and genetic risks not only of susceptibility but also of infectivity, therefore exploring a trait whose heritable variation is currently ignored in disease genetics and can greatly influence the spread of infectious diseases. Our proposed methodology offers potential impacts in areas such as livestock disease control through selective breeding and also in predicting and controlling the emergence of disease outbreaks in human populations
Genomics and disease resistance studies in livestock
AbstractThis paper considers the application of genetic and genomic techniques to disease resistance, the interpretation of data arising from such studies and the utilisation of the research outcomes to breed animals for enhanced resistance. Resistance and tolerance are defined and contrasted, factors affecting the analysis and interpretation of field data presented, and appropriate experimental designs discussed. These general principles are then applied to two detailed case studies, infectious pancreatic necrosis in Atlantic salmon and bovine tuberculosis in dairy cattle, and the lessons learnt are considered in detail. It is concluded that the rate limiting step in disease genetic studies will generally be provision of adequate phenotypic data, and its interpretation, rather than the genomic resources. Lastly, the importance of cross-disciplinary dialogue between the animal health and animal genetics communities is stressed
Can We Breed Cattle for Lower bovine TB Infectivity?
Publication history: Accepted - 22 November 2018; Published - 7 December 2018.Host resistance and infectivity are genetic traits affecting infectious disease transmission.
This Perspective discusses the potential exploitation of genetic variation in cattle
infectivity, in addition to resistance, to reduce the risk, and prevalence of bovine
tuberculosis (bTB). In bTB, variability in M. bovis shedding has been previously reported
in cattle and wildlife hosts (badgers and wild boars), but the observed differences
were attributed to dose and route of infection, rather than host genetics. This article
addresses the extent to which cattle infectivity may play a role in bTB transmission,
and discusses the feasibility, and potential benefits from incorporating infectivity into
breeding programmes. The underlying hypothesis is that bTB infectivity, like resistance,
is partly controlled by genetics. Identifying and reducing the number of cattle with
high genetic infectivity, could reduce further a major risk factor for herds exposed to
bTB. We outline evidence in support of this hypothesis and describe methodologies for
detecting and estimating genetic parameters for infectivity. Using genetic-epidemiological
predictionmodels we discuss the potential benefits of selection for reduced infectivity and
increased resistance in terms of practical field measures of epidemic risk and severity.
Simulations predict that adding infectivity to the breeding programme could enhance and
accelerate the reduction in breakdown risk compared to selection on resistance alone.
Therefore, given the recent launch of genetic evaluations for bTB resistance and the UK
government’s goal to eradicate bTB, it is timely to consider the potential of integrating
infectivity into breeding schemes.This work was carried out with funding from the Biotechnology
and Biological Sciences Research Council Institute Strategic
Programme grants BB/J004235/1 (ISP1) and BB/P013740/1
(ISP2) (OA, AD-W, GB and JW), and the European Union
FP7 project FISHBOOST (KBBE - 7-613611) (ST). GB was also
supported by the Rural and Environment Science and Analytical
Services Division of the Scottish Government