Physical and mechanical properties evaluations of a new engineered wood product

Finding alternative uses for small diameter raw materials is a critical problem throughout the United States. Insufficient markets for small diameter, southern yellow pine (Pinus spp.) trees from first plantation thinnings are impacting silvicultural practices on millions of acres of land. In western states, the lack of markets for small diameter ponderosa pine (Pinus ponderosa) and lodgepole pine (Pinus contorta) creates multiple problems in terms of excess material in the forest. This excess material enhances fire potential and reduces land management practices. This research deals with a new structural composite from small diameter raw logs using a technology called steampressed scrim lumber (SPSL). Mechanical and physical properties were performed and evaluated for each species. This research was conducted to evaluate these properties and to determine the suitability for commercialization

Three-dimensional water-wave scattering in two-layer fluids

We consider, using linear water-wave theory, three-dimensional problems concerning the interaction of waves with structures in a fluid which contains a layer of finite depth bounded above by a free surface and below by an infinite layer of fluid of greater density. For such a situation time-harmonic waves can propagate with two different wavenumbers K and k. In a single-layer fluid there are a number of reciprocity relations that exist connecting the various hydrodynamic quantities that arise, and these relations are systematically extended to the two-fluid case. The particular problems of wave radiation and scattering by a submerged sphere in either the upper or lower layer are then solved using multipole expansions

Scattering of oblique waves in a two-layer fluid

We consider problems based on linear water wave theory concerning the interaction of oblique waves with horizontal cylinders in a fluid consisting of a layer of finite depth bounded above by a free surface and below by an infinite layer of fluid of greater density. For such a situation time-harmonic waves can propagate with two different wavenumbers K and k. The particular problems of wave scattering by a horizontal circular cylinder in either the upper or lower layer are solved using multipole expansions

Chromosomal integration vectors allowing flexible expression of foreign genes in Campylobacter jejuni.

BACKGROUND: Campylobacter jejuni is a major cause of human gastroenteritis yet there is limited knowledge of how disease is caused. Molecular genetic approaches are vital for research into the virulence mechanisms of this important pathogen. Vectors that allow expression of genes in C. jejuni via recombination onto the chromosome are particularly useful for genetic complementation of insertional knockout mutants and more generally for expression of genes in particular C. jejuni host backgrounds. METHODS: A series of three vectors that allow integration of genes onto the C. jejuni chromosome were constructed by standard cloning techniques with expression driven from three different strong promoters. Following integration onto the C. jejuni chromosome expression levels were quantified by fluorescence measurements and cells visualized by fluorescence microscopy. RESULTS: We have created plasmid, pCJC1, designed for recombination-mediated delivery of genes onto the C. jejuni chromosome. This plasmid contains a chloramphenicol resistance cassette (cat) with upstream and downstream restriction sites, flanked by regions of the C. jejuni pseudogene Cj0223. Cloning of genes immediately upstream or downstream of the cat gene allows their subsequent introduction onto the C. jejuni chromosome within the pseudogene. Gene expression can be driven from the native gene promoter if included, or alternatively from the cat promoter if the gene is cloned downstream of, and in the same transcriptional orientation as cat. To provide increased and variable expression of genes from the C. jejuni chromosome we modified pCJC1 through incorporation of three relatively strong promoters from the porA, ureI and flaA genes of C. jejuni, Helicobacter pylori and Helicobacter pullorum respectively. These promoters along with their associated ribosome binding sites were cloned upstream of the cat gene on pCJC1 to create plasmids pCJC2, pCJC3 and pCJC4. To test their effectiveness, a green fluorescent protein (gfp) reporter gene was inserted downstream of each of the three promoters and following integration of promoter-gene fusions onto the C. jejuni host chromosome, expression levels were quantified. Expression from the porA promoter produced the highest fluorescence, from flaA intermediate levels and from ureI the lowest. Expression of gfp from the porA promoter enabled visualization by fluorescent microscopy of intracellular C. jejuni cells following invasion of HeLa cells. CONCLUSIONS: The plasmids constructed allow stable chromosomal expression of genes in C. jejuni and, depending on the promoter used, different expression levels were obtained making these plasmids useful tools for genetic complementation and high level expression

On the use of the Cauchy distribution to describe price fluctuations in R&D and other forms of real assets

An improved model for describing the returns of assets that result from R&D efforts is needed. Such a model may lead to better decision support tools to monetize the value of R&D activities for both public and private sector technology managers. Real option pricing methodologies are often used to gauge appropriate funding levels for assets such as R&D projects that contain large time-dependent uncertainties. A study of the commonly used Black-Scholes equation finds that the Gaussian distribution assumption used to describe the behaviour of the underlying assets’ fluctuations is not appropriate for R&D. This conclusion is based on a study of 43 military R&D projects and 100 micro-cap technology intensive small firms. A power law, such as the Cauchy distribution, is shown to be more accurate in describing fluctuations in returns on R&D investments. Using historical data we find that the Cauchy distribution is a better representation of the underlying assets’ behaviour in military R&D projects and in technology intensive firms with small market capitalization (i.e., single project firms that are commercializing R&D)

Full-Scale Test and Analysis Results of a PRSEUS Fuselage Panel to Assess Damage Containment Features

Integrally stitched composite technology is an area that shows promise in enhancing the structural integrity of aircraft and aerospace structures. The most recent generation of this technology is the Pultruded Rod Stitched Efficient Unitized Structure (PRSEUS) concept. The goal of the PRSEUS concept relevant to this test is to provide damage containment capability for composite structures while reducing overall structural weight. The National Aeronautics and Space Administration (NASA), the Federal Aviation Administration (FAA), and The Boeing Company have partnered in an effort to assess the damage containment features of a full-scale curved PRSEUS panel using the FAA Full-Scale Aircraft Structural Test Evaluation and Research (FASTER) facility. A single PRSEUS test panel was subjected to axial tension, internal pressure, and combined axial tension and internal pressure loads. The test results showed excellent performance of the PRSEUS concept. No growth of Barely Visible Impact Damage (BVID) was observed after ultimate loads were applied. With a two-bay notch severing the central stringer, damage was contained within the two-bay region well above the required limit load conditions. Catastrophic failure was well above the ultimate load level. Information describing the test panel and procedure has been previously presented, so this paper focuses on the experimental procedure, test results, nondestructive inspection results, and preliminary test and analysis correlation

Full-Scale Test and Analysis of a PRSEUS Fuselage Panel to Assess Damage-Containment Features

Stitched composite technology has the potential to substantially decrease structural weight through enhanced damage containment capabilities. The most recent generation of stitched composite technology, the Pultruded Rod Stitched Efficient Unitized Structure (PRSEUS) concept, has been shown to successfully arrest damage at the sub-component level through tension testing of a three stringer panel with damage in the form of a two-bay notch. In a joint effort undertaken by the National Aeronautics and Space Administration (NASA), the Federal Aviation Administration (FAA), and the Boeing Company, further studies are being conducted to characterize the damage containment features of the PRSEUS concept. A full-scale residual strength test will be performed on a fuselage panel to determine if the load capacity will meet strength, deformation, and damage tolerance requirements. A curved panel was designed, fabricated, and prepared for residual strength testing. A pre-test Finite Element Model (FEM) was developed using design allowables from previous test programs to predict test panel deformation characteristics and margins of safety. Three phases of testing with increasing damage severity include: (1) as manufactured; (2) barely visible impact damage (BVID) and visible impact damage (VID); and (3) discrete source damage (DSD) where the panel will be loaded to catastrophic failure. This paper presents the background information, test plan, and experimental procedure. This paper is the first of several future articles reporting the test preparations, results, and analysis conducted in the test program