A pilot of the use of the WAIS III in learning disability services

The present study is a pilot examining how a group of people (n=133) with a learning disability perform on the Wechsler Adult Intelligence Scale – Third Edition (1997). The study found that Full Scale IQ was not always predictive of performance on individual subtests or of performance on the Indices. Implications for clinical practice are discussed

Temperature Tolerance In Crustaceans: Critical Temperatures And The Heart

At certain upper and lower threshold temperatures (critical temperatures (Tc) crustaceans switch to anaerobic metabolism despite sufficient oxygen availability in the environment. I tested the hypothesis that failure of the heart at critical temperatures leads to insufficient oxygen delivery and subsequent anaerobiosis in peripheral tissues. I exposed rock crabs, Cancer irroratus, as whole animals, and their buffer-perfused semi-isolated hearts to a progressive temperature increase, while monitoring heart rate and lactate accumulation. The whole animals heart rate increased with temperature following a Q10 of 2.8. An abrupt decline in heart rate occurred at 25°C and lactate accumulation occurred between 25°C and 30°C. The semi-isolated hearts followed a Q10 of only 1.2 during temperature increase. A second set of semi-isolated hearts were perfused and paced at a heart rate comparable to the whole animals at each temperature. The lactate concentration in the paced semi-isolated hearts did not significantly increase. AMPK activity and HSP70 levels were measured to investigate cellular changes occurring at Tc. Preliminary data suggests they are not good indicating parameters of Tc for isolated hearts. I conclude that the whole animal’s Tc is 5°C lower than that of the heart muscle. Therefore, the onset of anaerobiosis in the peripheral tissues is not due to failure of the heart muscle. The processes within the whole animal that lead to anaerobiosis are set by other organ systems

Role of Schizosaccharomyces pombe RecQ homolog recombination and checkpoint genes in UV Damage tolerance

The cellular responses to DNA damage are complex and include direct DNA repair pathways that remove the damage and indirect damage responses which allow cells to survive DNA damage that has not been, or cannot be, removed. We have identified the gene mutated in the rad12.502 strain as a Schizosaccharomyces pombe recQ homolog. The same gene (designated rqh1) is also mutated in the hus2.22 mutant. We show that Rqh1 is involved in a DNA damage survival mechanism which prevents cell death when UV-induced DNA damage cannot be removed. This pathway also requires the correct functioning of the recombination machinery and the six checkpoint tad gene products plus the Cds1 kinase. Our data suggest that Rqh1 operates during S phase as part of a mechanism which prevents DNA damage causing cell lethality. This process may involve the bypass of DNA damage sites by the replication fork. Finally, in contrast with the reported literature, we do not find that rqh1 (rad12) mutant cells are defective in UV dimer endonuclease activity

Localisation of Theiler's Murine Encephalomyelitis virus non-structural proteins 2B, 2C, 2BC and 3A in BHK-21 cells, and the effect of amino acid substitutions in 2C on localisation and virus replication

The picornavirus family includes significant human and animal viruses such as poliovirus (PV), human rhinovirus (HRV) and foot-and-mouth-disease virus (FMDV). Current disease treatment and control strategies are limited by an incomplete understanding of the interactions between the non-structural, replicative picornavirus proteins and host cell components. To investigate these interactions, Theiler's murine encephalomyelitis virus (TMEV) 2B, 2C, 2BC and 3A proteins were transiently expressed in BHK-21 cells and detected by indirect immunostaining and laser-scanning or epifluorescence microscopy. The signal of the 2B protein overlapped with that of the ER marker protein, ERp60, as well as that of the peripheral Golgi marker protein, β-COP. The 2C protein overlapped with ERp60 in a faint reticular stain, and localised to large punctate structures that partially overlapped with β-COP at higher levels of expression. The 2BC protein located to large perinuclear structures that overlapped exclusively with β-COP. The TMEV 3A protein signal overlapped with both ERp60 and β-COP stains, in addition in cells expressing the 3A protein the ER appeared swollen and bulbous while the Golgi was dispersed in some cells. 2C and 2BC proteins with C-terminal deletions localised in the same manner as the wild type proteins indicating that the localisation signals that determine subcellular localisation of the proteins are within the N-terminal 60 amino acids of the 2C protein. The significance of the high degree of conservation of the N-terminal domain of the 2C protein throughout the Picornaviridae was investigated through the introduction of amino acid substitution mutations at highly conserved residues in the N-terminal domain of 2C into the viral cDNA. Upon transfection of the viral RNA into BHK-21 cells, it was observed that substitution of amino acid residues 8, 18 and 29 abolished the ability of TMEV to induce cytopathic effect (CPE), while substitution of residues 4, 14 and 23 only attenuated the ability of TMEV to induce CPE. To determine whether amino acid substitution mutations would affect the localisation of the 2C protein, 2C proteins with substitution mutations at amino acids 4, 8, 14, 18, 23 and 29 were transiently expressed in BHK-21 cells and detected by indirect imrnunostaining and examination by laser-scanning confocal and epifluorescence microscopy. The 2C mutant 4, 8 and 29 proteins showed slightly altered localisation patterns compared to the wild type protein with a significant portion of the proteins localising in a perinuclear stain suggesting possible localisation to the nuclear envelop. The 2C mutant 14 and 18 proteins localised to a diffuse pattern in BHK-21 cells while the 2C mutant 23 protein located to small punctate structures that partially overlapped with the ERp60 stain but were completely separate from the β-COP stain. Finally, a hydrophilic, antigenic region of the 2C protein was expressed in frame with an N-terminal GST tag and was successfully purified on a pilot-scale and detected by Western analysis. This 2C178 peptide will be used to generate antibodies against the 2C and 2BC proteins for use in future studies. This study has furthered our knowledge of the localisation of the picornavirus 2B, 2C, 2BC and 3A proteins in host cells and identified a possible link between this localisation and an ability of TMEV to replicate in BHK-21 cells

Remote Laboratories in Engineering Education: Trends in Students' Perceptions

Remotely accessible laboratories are an increasingly popular innovation in engineering education. Since 2001, The University of Technology, Sydney has implemented a number of remotely accessible laboratories. This paper presents an analysis of students' feedback responses to their use of the laboratories. The responses show that students not only appreciate the flexibility of the remote access option, but also that they feel that the remote option encourages them to take a deep learning approach to the material

Adapting a Remote Laboratory Architecture to Support Collaboration and Supervision

Interest in, and use of, remote laboratories has been rapidly growing. These laboratories provide remote access, via the internet, to real laboratory equipment. Under appropriate circumstances they can support or even replace traditional (proximal) laboratories, provide improved access at reduced cost, and encourage inter-institutional sharing of expensive resources. Most attention to date has been on the development of the core infrastructure that manages access and interaction, and to a lesser extent consideration of pedagogic issues such as which learning outcomes are best suited to this modality. There has however been a recent recognition of the importance of also considering how collaboration and supervision can also be supported. In this paper we discuss a novel approach to the integration of support for multi-user distributed access to a single laboratory instance. The approach retains the benefits of the lightweight client inherent in the underlying architecture

There is no Other Monkey in the Mirror for Spider Monkeys (Ateles geoffroyi)

©American Psychological Association, 2020. This paper is not the copy of record and may not exactly replicate the authoritative document published in the APA journal. Please do not copy or cite without author's permission. The final article is available, upon publication, at: https://doi.org/10.1037/com0000243Mirror self-recognition (MSR), usually considered a marker of self-awareness, occurs in several species and may reflect a capacity that has evolved in small incremental steps. In line with research on human development and building on previous research adopting a gradualist framework, we categorized the initial mirror responses of naïve spider monkeys (Ateles geoffroyi) according to four levels. We compared social, exploratory, contingent and self-exploratory responses to a mirror and faux mirror during three short trials. If spider monkeys respond as most monkey species, we predicted they would perform at level 0, mainly showing social behavior toward their mirror-image. However, because spider monkeys show enhancement of certain cognitive skills comparable to those of great ape species, we predicted that they would perform at level 1a (showing exploratory behavior) or 1b (showing contingent behavior). GLMMs revealed that monkeys looked behind and visually inspected the mirror significantly more in the mirror than the faux mirror condition. Although the monkeys engaged in contingent body movements at the mirror, this trend was not significant. Strikingly, they showed no social behaviors toward their mirror-image. We also measured self-scratching as an indicator of anxiety and found no differences in frequencies of self-scratching between conditions. Therefore, in contrast to most findings on other species, spider monkeys did not treat their image as another monkey during their initial exposure to the mirror. In fact, they reached at least level 1a within minutes of mirror exposure. These responses recommend spider monkeys as good candidates for further explorations into monkey self-recognition

TEACHER COLLABORATIVE INQUIRY IN ONTARIO ELEMENTARY SCHOOLS: AN ANALYSIS OF PROVINCIAL AND SCHOOL BOARD POLICIES AND SUPPORT DOCUMENTS

Collaborative inquiry (CI) has emerged as a dominant structure for educators’ professional learning in the 21st century. The purpose of this paper is to analyze publicly available documents and policies related to CI in Ontario in order to better understand the documentary scope and spread of this professional learning model in the province. We begin by defining the parameters of CI as a dominant professional learning model before detailing our methodology for selecting and analyzing CI policies and documents at both ministry and school board levels. In our subsequent analysis, we enumerate emergent themes and findings and offer three sample case studies that illustrate how school boards in the province are documenting their experiences with CI. Finally, we conclude with a discussion of potential tensions within current CI policies as a basis for future research and policy development.

Social network analysis of a chimpanzee (Pan troglodytes) group in captivity following the integration of a new adult member

Management of primates in captivity often presents the challenge of introducing new individuals into a group, and research investigating the stability of the social network in the medium-term after the introduction can help inform management decisions. We investigated the behavior of a group of chimpanzees (Pan troglodytes) housed at Chester Zoo, UK over 12 months (divided into three periods of four months) following the introduction of a new adult female. We recorded grooming, proximity, other affiliative behaviors and agonistic behaviors and used Social Network Analysis to investigate the stability, reciprocity and structure of the group, to examine the effect of rearing history on grooming network position and the role of sex in agonistic behavior. Both the grooming and agonistic networks correlated across all three periods, while affiliative networks correlated only between periods two and three. Males had significantly higher out-degree centrality in agonistic behaviors than females, indicating that they carried out agonistic behaviors more often than females. There was no significant difference in centrality between hand-reared and mother-reared chimpanzees. Overall, the group structure was stable and cohesive during the first year after the introduction of the new female, suggesting that this change did not destabilize the group. Our findings highlight the utility of Social Network Analysis in the study of primate sociality in captivity, and how it can be used to better understand primate behavior following the integration of new individuals

Establishment reality vs maintenance reality: how real is real enough?

Remote and virtual laboratories are increasingly prevalent alternatives to the face-to-face laboratory experience; however, the question of their learning outcomes is yet to be fully investigated. There are many presumptions regarding the effectiveness of these approaches; foremost amongst these assumptions is that the experience must be 'real' to be effective. Embedding reality into a remote or virtual laboratory can be an expensive and time-consuming task. Significant efforts have been expended to create 3D VRML models of laboratory equipment, allowing students to pan, zoom and tilt their perspective as they see fit. Multiple camera angles have been embedded into remote interfaces to provide an increased sense of 'realness'. This paper draws upon the literature in the field to show that the necessary threshold for reality varies depending upon how the students are interacting with the equipment. There is one threshold for when they first interact - the establishment reality - which allows the students to familiarise themselves with the laboratory equipment, and to build their mental model of the experience. There is, however, a second, lower, threshold - the maintenance reality - that is necessary for the students' ongoing operation of the equipment. Students' usage patterns rely upon a limited subset of the available functionality, focusing upon only some aspects of the reality that has been originally established. The two threshold model presented in this paper provides a new insight for the development of virtual laboratories in the future