PVA−DNA Cryogel Membranes: Characterization, Swelling, and Transport Studies

Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for different types of matrixes: an extended one in a more crystalline network and a globular one in a more amorphous one. Different associations of base pairs have also been observed. PVA cryogel crystallinity could be tailored by the cryogel contact with different salt solutions. Cryogel surface (scanning electron microscopy) and bulk morphology (porosimetry), swelling, DNA retention, and delivery kinetics have also been studied. All these investigations clearly show strong interactions between PVA and DNA

Intense tera-hertz laser driven proton acceleration in plasmas

We investigate the acceleration of a proton beam driven by intense tera-hertz (THz) laser field from a near critical density hydrogen plasma. Two-dimension-in-space and three-dimension-in-velocity particle-in-cell simulation results show that a relatively long wavelength and an intense THz laser can be employed for proton acceleration to high energies from near critical density plasmas. We adopt here the electromagnetic field in a long wavelength (0.33 THz) regime in contrast to the optical and/or near infrared wavelength regime, which offers distinct advantages due to their long wavelength (k ¼ 350 lm), such as the k2 scaling of the electron ponderomotive energy. Simulation study delineates the evolution of THz laser field in a near critical plasma reflecting the enhancement in the electric field of laser, which can be of high relevance for staged or post ion acceleration

BPS partition functions in N = 4 Yang-Mills theory on T^4

We consider N = 4 Yang-Mills theory on a flat four-torus with the R-symmetry current coupled to a flat background connection. The partition function depends on the coupling constant of the theory, but when it is expanded in a power series in the R-symmetry connection around the loci at which one of the supersymmetries is unbroken, the constant and linear terms are in fact independent of the coupling constant and can be computed at weak coupling for all non-trivial 't Hooft fluxes. The case of a trivial 't Hooft flux is difficult because of infrared problems, but the corresponding terms in the partition function are uniquely determined by S-duality.Comment: 23 pages, v2 Minor correction

On infrastructure for facilitation of inner source in small development teams

The phenomenon of adopting open source software development practices in a corporate environment is known by many names, one being inner source. The objective of this study is to investigate how an organization consisting of small development teams can benet from adopting inner source and assess the level of applicability. The research has been conducted as a case study at a software development company. Data collection was carried out through interviews and a series of focus group meetings, and then analyzed by mapping it to an available framework. The analysis shows that the organization possesses potential, and also identied a number of challenges and benets of special importance to the case company. To address these challenges, the case study synthesized the organizational and infrastructural needs of the organization in a requirements specication describing a technical infrastructure, also known as a software forge, with an adapted organizational context and work process

Chiral molecules split light: Reflection and refraction in a chiral liquid

A light beam changes direction as it enters a liquid at an angle from another medium, such as air. Should the liquid contain molecules that lack mirror symmetry, then it has been predicted by Fresnel that the light beam will not only change direction, but will actually split into two separate beams with a small difference in the respective angles of refraction. Here we report the observation of this phenomenon. We also demonstrate that the angle of reflection does not equal the angle of incidence in a chiral medium. Unlike conventional optical rotation, which depends on the path-length through the sample, the reported reflection and refraction phenomena arise within a few wavelengths at the interface and thereby suggest a new approach to polarimetry that can be used in microfluidic volumes

Adenovirus 11p downregulates CD46 early in infection

AbstractAdenovirus 11 prototype (Ad11p), belonging to species B, uses CD46 as an attachment receptor. CD46, a complement regulatory molecule, is expressed on all human nucleated cells. We show here that Ad11p virions downregulate CD46 on the surface of K562 cells as early as 5min p.i. Specific binding to CD46 by the Ad11p fiber knob was required to mediate downregulation. The complement regulatory factors CD55 and CD59 were also reduced to a significant extent as a consequence of Ad11p binding to K562 cells. In contrast, binding of Ad7p did not result in downregulation of CD46 early in infection. Thus, the presumed interaction between Ad7p and CD46 did not have the same consequences as the Ad11p–CD46 interaction, the latter virus (Ad11p) being a promising gene therapy vector candidate. These findings may lead to a better understanding of the pathogenesis of species B adenovirus infections

Kvalitetsindikatoren 30 dagers reinnleggelse etter sykehusopphold. Resultater for sykehus og kommuner 2014: Kvalitetsmåling

Folkehelseinstituttet beregner kvalitetsindikatorer for risikojustert sannsynlighet for reinnleggelse innen 30 dager etter utskrivning fra sykehus for eldre pasienter. Indikatorene inngår i det nasjonale kvalitetsindikatorsystemet som forvaltes av Helsedirektoratet

Cell-Nanoparticle Interactions at (Sub)-Nanometer Resolution Analyzed by Electron Microscopy and Correlative Coherent Anti-Stokes Raman Scattering

A wide variety of nanoparticles are playing an increasingly important role in drug delivery. Label-free imaging techniques are especially desirable to follow the cellular uptake and intracellular fate of nanoparticles. The combined correlative use of different techniques, each with unique advantages, facilitates more detailed investigation about such interactions. The synergistic use of correlative coherent anti-Stokes Raman scattering and electron microscopy (C-CARS-EM) imaging offers label-free, chemically-specific, and (sub)-nanometer spatial resolution for studying nanoparticle uptake into cells as demonstrated in the current study. Coherent anti-Stokes Raman scattering (CARS) microscopy offers chemically-specific (sub)micron spatial resolution imaging without fluorescent labels while transmission electron microscopy (TEM) offers (sub)-nanometer scale spatial resolution and thus visualization of precise nanoparticle localization at the sub-cellular level. This proof-of-concept imaging platform with unlabeled drug nanocrystals and macrophage cells revealed good colocalization between the CARS signal and electron dense nanocrystals in TEM images. The correlative TEM images revealed subcellular localization of nanocrystals inside membrane bound vesicles, showing multivesicular body (MVB)-like morphology typical for late endosomes (LEs), endolysosomes, and phagolysosomes. C-CARS-EM imaging has much potential to study the interactions between a wide range of nanoparticles and cells with high precision and confidence.Peer reviewe