STIM1/Orai1-mediated store-operated Ca2+ entry: the tip of the iceberg

Highly efficient mechanisms regulate intracellular calcium (Ca2+) levels. The recent discovery of new components linking intracellular Ca2+ stores to plasma membrane Ca2+ entry channels has brought new insight into the understanding of Ca2+ homeostasis. Stromal interaction molecule 1 (STIM1) was identified as a Ca2+ sensor essential for Ca2+ store depletion-triggered Ca2+ influx. Orai1 was recognized as being an essential component for the Ca2+ release-activated Ca2+ (CRAC) channel. Together, these proteins participate in store-operated Ca2+ channel function. Defective regulation of intracellular Ca2+ is a hallmark of several diseases. In this review, we focus on Ca2+ regulation by the STIM1/Orai1 pathway and review evidence that implicates STIM1/Orai1 in several pathological conditions including cardiovascular and pulmonary diseases, among others

Impact of dietary plant protein levels on the consumer acceptability of senegalese sole (Solea senegalensis Kaup, 1858)

The aim of the present work was to assess the impact of different vegetable diets in the sensory acceptability of Senegalese sole.This work was carried out by Project PROAMBIENTE for enhancing SMEs organizational and management structure to enter foreign markets. PROAMBIENTE has the financial support of Quadro de Referência Estratégico Nacional – QREN and Programa Operacional Regional do Norte – ON2, supported by the European fund for regional development FEDER

Curvatura Da Superfície E Seu Efeito Na Variabilidade Espacial Dos Atributos Do Solo, Pinheiral - Rj/br

Understanding the spatial variability of soil chemical and physical attributes is important for improving management practices and soil conservation. In turn, the spatial variability of soil properties results from variation in morphological relief characteristics. Thus, the purpose of this study was to evaluate the influence of landform curvature on the spatial variability of soil chemical and physical attributes in the Mar de Morros region (Pinheiral-Rio de Janeiro State, Brazil). Two adjacent landforms were selected with convex and concave curvature and sampled in a regularly spaced grid of 10 meters. A total of 56 soil samples (0-5 cm depth) from the two landforms were collected and analyzed for physical and chemical attributes. The data were analyzed using descriptive statistics and geostatistics. All chemical attributes showed random patterns of spatial variability in both landforms. The concave landform had higher values of pH and potassium and lower values of aluminum than the convex landform. In contrast, silt content showed spatial dependence in both the concave and convex landforms. Bulk density and clay showed spatial dependence in the convex landform. Bulk density and silt content increased from the shoulder to the footslope of both landforms. The results show that, for this study area, landform curvature has more influence on the spatial dependence of soil physical attributes than of soil chemical properties. © 2016, Federal University of Lavras. All rights reserved.22443143

Spectrophotometric determination of carbon dioxide and sulphur dioxide in wines by flow injection

Flow injection analysis (FIA) methods for the spectrophotometric determination of CO2 and SO2 in wines are described. The determination of CO2 is based on the colour change of a low capacity buffer (containing an acid-base indicator) due to the dissolved carbon dioxide. The determination of SO2 is based on the decoloration of malachite green by sulphur dioxide. Two FIA manifolds are presented; one for the determination of CO2 in sparkling wines and another for the simultaneous determination of CO2 and SO2 in table wines. The analytes are isolated inside the manifold from the sample matrix using gas-diffusion units. Regression equations (FIA versus reference methods) showed no statistical difference, at 95 % confidence level, between the two sets of results for both determinations; additionally, for the determination of CO2, recovery values between 93.5 % and 111 % were found. RSD lower than 4.5 % for SO2 and 2.4 % for the CO2 determination were found. The sampling rates achieved were: 30 h–1 for the uniparametric system and 40 h–1 for the biparametric system. The single determination manifold is applicable in the concentration ranges of 0.5 to 4 g L–1 of CO2, and the simultaneous determination manifold in the range of 0.25 to 3 g L–1 of CO2 and 0.05 to 0.3 g L–1 of SO

Dried brewers grains in growing rabbits: nutritional value and effects on performance

[EN] Two assays were carried out to determine i) the nutritional value of dried brewers’ grains and ii) the effects of inclusion of this ingredient in growing rabbit diets on animal performance and economic performance of the breeding unit. In the digestibility assay, a total of 28 male rabbits were distributed in 2 groups differing in the diet offered to animals: a reference diet (35.51% neutral detergent fibre and 16.50% crude protein [CP]) and a test diet (60% of reference diet and 40% of dried brewers grains). The dried brewers’ grain contained 37.9% of CP and 3371 kcal digestible energy/kg dry matter. In the performance study, 80 weaned rabbits (40 males and 40 females) were allotted at 40 d of age to 5 groups differing in the inclusion levels of dried brewers’ grains (0, 7, 14, 21 and 28%) from 40 d to 90 d of age. Inclusion of dried brewers’ grains did not affect the live weight at 90 d, the feed intake between 40 d and 90 d or the dressing percentage of rabbits (on average 223 g, 96 g/d and 51.3%, respectively). There was no effect of diet on the meat quality parameters (69.5% water holding capacity, 25.6% cooking loss, 3.4 kg/cm2 Warner-Bratzler shear force and pH 5.70) and inclusion levels above 14% reduced the feed cost (–18%; P<0.001), while inclusion above 21% improved net income (+32%; P<0.001). In conclusion, these results suggest that the use of dried brewers’ grains in diets for growing rabbits could improve the economic performance of the production system without impairing the animals’ performance.Lima, P.; Watanabe, P.; Cândido, R.; Ferreira, A.; Vieira, A.; Rodrigues, B.; Nascimento, G.... (2017). Dried brewers grains in growing rabbits: nutritional value and effects on performance. World Rabbit Science. 25(3):251-260. doi:10.4995/wrs.2017.6813.SWORD251260253Albuquerque, D. M. N., Lopes, J. B., Klein Junior, M. H., Merval, R. R., Silva, F. E. S., & Teixeira, M. P. F. (2011). Resíduo desidratado de cervejaria para suínos em terminação. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(2), 465-472. doi:10.1590/s0102-09352011000200026AOAC International. 2005. Official methods of analysis of Association Official Analytical Chemists International. AOAC, Arlington VA. USA.Briganó, M. V., Pacheco, G. D., Bridi, A. M., Oba, A., Fonseca, N. A. N., & Silva, C. A. da. (2008). Desempenho e características de carcaça de suínos submetidos a diferentes programas de restrição alimentar na fase dos 30 aos 118 kg. Revista Brasileira de Zootecnia, 37(8), 1398-1404. doi:10.1590/s1516-35982008000800009De Blas, J. C., Rodriguez, C. A., Bacha, F., Fernandez, R., & Abad-Guamán, R. (2015). Nutritive value of co-products derived from olivecake in rabbit feeding. World Rabbit Science, 23(4), 255. doi:10.4995/wrs.2015.4036Etchu K.A., Humbu M.E., Ndamukong K.J.N., Agbor E.B. 2012. Effect of varying levels of brewers' dried grain on the growth performance of weaner rabbits (Oryctolagus Cuniculus). Greener J. Agric. Sci., 2: 237-245.Fazano A.R.T., Zinsly C.F., Mattos W.R.S., Packer I.H. 1986. Digestibilidade e valor biológico da proteína da levedura seca (Saccharomyces spp.) e do farelo de soja para coelhos. Boletim de Indústria Animal, 46: 185-191.Lounaouci-Ouyaed G., Lakabi-ioualitene D., Berchiche M., Lebas F. 2008. Field beans and brewers grain as protein source for growing rabbits in Algeria: first results on growth and carcass quality. Nutrition and Digestive Physiology. In: 9th World Rabbit Congress, June 10-13, Verona, Italy. 723-728.Matterson L.D., Potter L.M., Stutz M.W., Singsen E.P. 1965. The metabolizable energy of feed ingredients for chickens. Storrs: University of Connecticut; Agricultural Experiment Station Research Report, 11: 11.National Research Council. 2007. Nutrient requirements of small ruminants: sheep, goats, cervids, and New World camelids. Washington, D.C.: National Academic Press, 292.Partridge G., Wyatt C. 1995. More flexibility with new generation of enzymes. World Poultry,11: 17-21.SAS 2000. SAS/STAT User's Guide (Release 6.12). SAS Inst. Inc., Cary NC, EUA.Villamide, M. J. (1996). Methods of energy evaluation of feed ingredients for rabbits and their accuracy. Animal Feed Science and Technology, 57(3), 211-223. doi:10.1016/0377-8401(95)00855-

Manejo De Bacias Hidrográficas E Sua Influência Sobre Os Recursos Hídricos

Studies on the use and occupation of soil explored by various segments of urban, rural and industrial societies, associated to the watershed characteristics are extremely important for water resources management and sustainability. Therefore, this work aimed to evaluate the influence of different managements of soil use and occupation associated to declivity and superficial flow water resources of watersheds located in Triângulo Mineiro – MG. The study was conducted by monitoring the flow and sediment in 4 basins from September 2013 to October 2014, besides the determination of soil use and occupation, declivity and superficial flow during this time. The obtained mathematical models showed that the ratio of the different soil uses and occupations interferes with the specific production and sediment concentration and the specific flow of watersheds as well, while the amount of superficial flow interferes with the specific sediment production. The simulations results of the management of watersheds’ use and occupation showed that the conservation of native forests, the conservationist techniques of soil use and the minimization techniques of diffuse transport from urban areas are essential for keeping water availability and quality. © 2016, Universidade Estadual Paulista - UNESP. All rights reserved.21223925

Einstein energy associated with the Friedmann -Robertson -Walker metric

Following Einstein's definition of Lagrangian density and gravitational field energy density (Einstein, A., Ann. Phys. Lpz., 49, 806 (1916); Einstein, A., Phys. Z., 19, 115 (1918); Pauli, W., {\it Theory of Relativity}, B.I. Publications, Mumbai, 1963, Trans. by G. Field), Tolman derived a general formula for the total matter plus gravitational field energy ($P_0$) of an arbitrary system (Tolman, R.C., Phys. Rev., 35(8), 875 (1930); Tolman, R.C., {\it Relativity, Thermodynamics & Cosmology}, Clarendon Press, Oxford, 1962)); Xulu, S.S., arXiv:hep-th/0308070 (2003)). For a static isolated system, in quasi-Cartesian coordinates, this formula leads to the well known result $P_0 = \int \sqrt{-g} (T_0^0 - T_1^1 -T_2^2 -T_3^3) ~d^3 x$, where $g$ is the determinant of the metric tensor and $T^a_b$ is the energy momentum tensor of the {\em matter}. Though in the literature, this is known as "Tolman Mass", it must be realized that this is essentially "Einstein Mass" because the underlying pseudo-tensor here is due to Einstein. In fact, Landau -Lifshitz obtained the same expression for the "inertial mass" of a static isolated system without using any pseudo-tensor at all and which points to physical significance and correctness of Einstein Mass (Landau, L.D., and Lifshitz, E.M., {\it The Classical Theory of Fields}, Pergamon Press, Oxford, 2th ed., 1962)! For the first time we apply this general formula to find an expression for $P_0$ for the Friedmann- Robertson -Walker (FRW) metric by using the same quasi-Cartesian basis. As we analyze this new result, physically, a spatially flat model having no cosmological constant is suggested. Eventually, it is seen that conservation of $P_0$ is honoured only in the a static limit.Comment: By mistake a marginally different earlier version was loaded, now the journal version is uploade

Nonsingular FRW cosmology and nonlinear electrodynamics

The possibility to avoid the cosmic initial singularity as a consequence of nonlinear effects on the Maxwell eletromagnetic theory is discussed. For a flat FRW geometry we derive the general nonsingular solution supported by a magnetic field plus a cosmic fluid and a nonvanishing vacuum energy density. The nonsingular behavior of solutions with a time-dependent $\Lambda(t)$-term are also examined. As a general result, it is found that the functional dependence of $\Lambda(t)$ can uniquely be determined only if the magnetic field remains constant. All these models are examples of bouncing universes which may exhibit an inflationary dynamics driven by the nonlinear corrections of the magnetic field.Comment: 20 pages, 7 figure