Complicações necrosantes e intercorrências na rinomodelação com ácido hialurônico

Objetivo: Descrever os efeitos adversos, intercorrências e complicações do preenchimento com ácido hialurônico na região de nariz, indicando possíveis causas para os problemas apresentados após o procedimento. Metodologia: Trata-se de uma revisão integrativa realizada nas seguintes bases de dados: Medline via PubMed, SciELO e Lilacs, com artigos incluídos no período 2013 a 2023, utilizando os descritores, Ácido hialurônico; Necrose; Preenchedores dérmicos; Nariz. A busca integrada foi realizada unindo os descritores com o operador booleano “AND”. Foram analisadas 9 publicações. Resultados: Foi realizada a identificação dos trabalhos selecionados, elencando-se os dados relacionados à título, autor, ano de publicação, tipo de estudo, metodologia, principais intercorrências encontradas e a conclusão do estudo. Após análise dos estudos, observou-se que a ocorrência de complicações após a aplicação de ácido hialurônico não é frequente. Para que não ocorra o agravamento, é necessário que os primeiros sinais e sintomas sejam analisados para assim seguir com o protocolo mais adequado a fim de prevenir ou reverter. Conclusão: Seguindo passos simples de segurança e tendo um conhecimento anatômico refinado, os preenchedores podem ser uma boa ferramenta para um aprimoramento seguro e abrangente da rinomodelação

Atividade antimicrobiana e anti-inflamatória da Anadenanthera colubrina (Vell.) Brenan

The present study aimed to analyze the antimicrobial and anti-inflammatory activity of A. colubrina (Vell.) Brenan extract through a panel of biological activities. The hydroalcoholic extract of A. colubrina was obtained by maceration, which was rotary evaporated and lyophilized. Minimal Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM) tests were performed microdilution against Candida albicans (ATCC 10231 and clinical strain), Candida tropicalis (ATCC 750) and Candida krusei (ATCC 6258). Anti-inflammatory activity was verified by induced paw edema. According to the tests performed, A. colubrina extract showed strong antifungal activity against C. albicans (ATCC 10231) and C. krusei (MIC: 7.81 μg / mL and 31.25 μg / mL, respectively). For the clinical strain of C. albicans and C. tropicalis, antifungal activity was considered weak (MIC: 500 μg / mL and> 2000 μg / mL, respectively). For the CFM tests, the extract showed fungistatic activity on C. albicans strains (ATCC 10231 and clinical strain) and C. tropicalis (CFM:> 1000 μg / mL,> 2000 μg / mL,> 5000 μg / mL, respectively), with fungicidal effect on C. krusei (CFM: 31.25 μg / mL). About the anti-inflammatory activity, among the concentrations of the extract tested, the one administered at the lower dosage presented a greater reduction of the induced paw edema. According to the above, the anti-Candida potential of A. colubrina. It is interesting to carry out studies that can confirm the anti-inflammatory potential of the test plant.Este estudio tuvo como objetivo analizar la actividad antimicrobiana y antiinflamatoria del extracto de A. colubrina (Vell.) Brenan a través de un panel de actividades biológicas. El extracto hidroalcohólico de A. colubrina se obtuvo por maceración, se evaporó por rotación y se liofilizó. Las pruebas de concentración mínima inhibitoria (MIC) y concentración mínima de fungicida (CFM) se realizaron contra Candida albicans (ATCC 10231 y cepa clínica), Candida tropicalis (ATCC 750) y Candida krusei (ATCC 6258) mediante microdilución. La actividad antiinflamatoria se verificó mediante edema de la pata inducido. Dadas las pruebas realizadas, el extracto de A. colubrina mostró una fuerte actividad antifúngica contra C. albicans (ATCC 10231) y C. krusei (MIC: 7,81 µg / ml y 31,25 µg / ml, respectivamente). Para la cepa clínica de C. albicans y C. tropicalis, la actividad antifúngica se consideró débil (MIC: 500 µg / ml y> 2000 µg / ml, respectivamente). Para las pruebas de CFM, el extracto mostró actividad fungistática en C. albicans (ATCC 10231 y cepa clínica) y C. tropicalis (CFM:> 1000 µg / ml,> 2000 µg / ml,> 5000 µg / ml, respectivamente), observando el efecto fungicida solo en C. krusei (CFM: 31.25 µg / mL). Con respecto a la actividad antiinflamatoria, entre las concentraciones del extracto analizado, el administrado en la dosis más baja presentó una mayor reducción del edema inducido de la pata. En consecuencia, el potencial anti-Candida de A. colubrina. Es interesante realizar estudios que puedan confirmar el potencial antiinflamatorio de la planta bajo prueba.O presente estudo teve como objetivo analisar a atividade antimicrobiana e anti-inflamatória do extrato da A. colubrina (Vell.) Brenan através de um painel de atividades biológicas. O extrato hidroalcoólico da A. colubrina foi obtido através de maceração, o qual rotaevaporado e liofilizado. Foram realizados testes de Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) contra Candida albicans (ATCC 10231 e cepa clínica), Candida tropicalis (ATCC 750) e Candida krusei (ATCC 6258), através da microdiluição. A atividade anti-inflamatória foi verificada através do edema de pata induzido. Diante dos testes realizados, o extrato da A. colubrina demonstrou forte atividade antifúngica contra a C. albicans (ATCC 10231) e a C. krusei (CIM: 7,81 µg/mL e 31,25 µg/mL, respectivamente). Para a cepa clínica da C. albicans e C. tropicalis a atividade antifúngica foi considerada fraca (CIM: 500 µg/mL e >2000 µg/mL, respectivamente). Para os testes de CFM, o extrato demonstrou atividade fungistática sobre as cepas C. albicans, (ATCC 10231 e cepa clínica) e C. tropicalis (CFM: > 1000 µg/mL, > 2000 µg/mL, > 5000 µg/mL, respectivamente), observando-se efeito fungicida apenas sobre a C. krusei (CFM: 31,25 µg/mL). Em relação à atividade anti-inflamatória, dentre as concentrações do extrato testadas, aquela administrada na menor dosagem apresentou maior redução do edema de pata induzido. De acordo com o exposto, é evidente o potencial anti-Candida da A. colubrina. É interessante a realização de estudos que possam confirmar o potencial anti-inflamatório da planta em teste

Chemical Composition, Antibacterial and Antifungal Potential of an Extract From the Leaves of Guapira Graciliflora Mart. Against Oral Microorganisms of Dental Interest

Objective: To perform an in vitro analysis of antibacterial and antifungal potential of an alcoholic extract from the leaves of Guapira Graciliflora Mart. against oral microorganisms and determine its chemical composition. Material and Methods: A hydroalcoholic extract of the leaves form G.  graciliflora was obtained through maceration, vacuum concentration and freeze-drying. Antibacterial and antifungal activities were evaluated against Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, Streptococcus parasanguinis, Streptococcus mitis and strains of Candida albicans using broth microdilution method. Phytochemical analysis determined the total phenolic compounds, protein concentration and total of sugars present in the extract. Results: G. Graciliflora demonstrated antifungal activity against the LM 11 and LM 410 clinical isolates of C. albicans (MIC 0.5 mg/mL and 2 mg/mL, respectively). The other microorganisms tested were resistant to the extract. The phytochemical analysis revealed 3% proteins, 13% total sugars and 17% phenolic compounds. Conclusion: G. Graciliflora has antifungal activity against clinical strains of C. albicans and exhibits proteins, sugars and phenolic compounds in its chemical composition

Antimicrobial and antiproliferative potential of Anadenanthera colubrina (vell.) Brenan

The aim of the present study was to perform an in vitro analysis of the antimicrobial and antiproliferative potential of an extract from Anadenanthera colubrina (Vell.) Brenan (angico) and chemically characterize the crude extract. Antimicrobial action was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration, and the inhibition of formation to oral biofilm. Cell morphology was determined through scanning electron microscopy (SEM). Six strains of tumor cells were used for the determination of antiproliferative potential. The extract demonstrated strong antifungal activity against Candida albicans ATCC 18804 (MIC = 0.031 mg/mL), with similar activity found regarding the ethyl acetate fraction. The extract and active fraction also demonstrated the capacity to inhibit the formation of Candida albicans to oral biofilm after 48 hours, with median values equal to or greater than the control group, but the difference did not achieve statistical significance (P > 0.05). SEM revealed alterations in the cell morphology of the yeast. Regarding antiproliferative activity, the extract demonstrated cytostatic potential in all strains tested. The present findings suggest strong antifungal potential for Anadenanthera colubrina (Vell.) Brenan as well as a tendency toward diminishing the growth of human tumor cells.The aim of the present study was to perform an in vitro analysis of the antimicrobial and antiproliferative potential of an extract from Anadenanthera colubrina (Vell.) Brenan (angico) and chemically characterize the crude extract. Antimicrobial action was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration, and the inhibition of formation to oral biofilm. Cell morphology was determined through scanning electron microscopy (SEM). Six strains of tumor cells were used for the determination of antiproliferative potential. The extract demonstrated strong antifungal activity against Candida albicans ATCC 18804 ( mg/mL), with similar activity found regarding the ethyl acetate fraction. The extract and active fraction also demonstrated the capacity to inhibit the formation of Candida albicans to oral biofilm after 48 hours, with median values equal to or greater than the control group, but the difference did not achieve statistical significance . SEM revealed alterations in the cell morphology of the yeast. Regarding antiproliferative activity, the extract demonstrated cytostatic potential in all strains tested. The present findings suggest strong antifungal potential for Anadenanthera colubrina (Vell.) Brenan as well as a tendency toward diminishing the growth of human tumor cells201

Atividade antimicrobiana e anti-inflamatória da Anadenanthera colubrina (Vell.) Brenan

The present study aimed to analyze the antimicrobial and anti-inflammatory activity of A. colubrina (Vell.) Brenan extract through a panel of biological activities. The hydroalcoholic extract of A. colubrina was obtained by maceration, which was rotary evaporated and lyophilized. Minimal Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM) tests were performed microdilution against Candida albicans (ATCC 10231 and clinical strain), Candida tropicalis (ATCC 750) and Candida krusei (ATCC 6258). Anti-inflammatory activity was verified by induced paw edema. According to the tests performed, A. colubrina extract showed strong antifungal activity against C. albicans (ATCC 10231) and C. krusei (MIC: 7.81 μg / mL and 31.25 μg / mL, respectively). For the clinical strain of C. albicans and C. tropicalis, antifungal activity was considered weak (MIC: 500 μg / mL and> 2000 μg / mL, respectively). For the CFM tests, the extract showed fungistatic activity on C. albicans strains (ATCC 10231 and clinical strain) and C. tropicalis (CFM:> 1000 μg / mL,> 2000 μg / mL,> 5000 μg / mL, respectively), with fungicidal effect on C. krusei (CFM: 31.25 μg / mL). About the anti-inflammatory activity, among the concentrations of the extract tested, the one administered at the lower dosage presented a greater reduction of the induced paw edema. According to the above, the anti-Candida potential of A. colubrina. It is interesting to carry out studies that can confirm the anti-inflammatory potential of the test plant.O presente estudo teve como objetivo analisar a atividade antimicrobiana e anti-inflamatória do extrato da A. colubrina (Vell.) Brenan através de um painel de atividades biológicas. O extrato hidroalcoólico da A. colubrina foi obtido através de maceração, o qual rotaevaporado e liofilizado. Foram realizados testes de Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) contra Candida albicans (ATCC 10231 e cepa clínica), Candida tropicalis (ATCC 750) e Candida krusei (ATCC 6258), através da microdiluição. A atividade anti-inflamatória foi verificada através do edema de pata induzido. Diante dos testes realizados, o extrato da A. colubrina demonstrou forte atividade antifúngica contra a C. albicans (ATCC 10231) e a C. krusei (CIM: 7,81 µg/mL e 31,25 µg/mL, respectivamente). Para a cepa clínica da C. albicans e C. tropicalis a atividade antifúngica foi considerada fraca (CIM: 500 µg/mL e >2000 µg/mL, respectivamente). Para os testes de CFM, o extrato demonstrou atividade fungistática sobre as cepas C. albicans, (ATCC 10231 e cepa clínica) e C. tropicalis (CFM: > 1000 µg/mL, > 2000 µg/mL, > 5000 µg/mL, respectivamente), observando-se efeito fungicida apenas sobre a C. krusei (CFM: 31,25 µg/mL). Em relação à atividade anti-inflamatória, dentre as concentrações do extrato testadas, aquela administrada na menor dosagem apresentou maior redução do edema de pata induzido. De acordo com o exposto, é evidente o potencial anti-Candida da A. colubrina. É interessante a realização de estudos que possam confirmar o potencial anti-inflamatório da planta em teste.Este estudio tuvo como objetivo analizar la actividad antimicrobiana y antiinflamatoria del extracto de A. colubrina (Vell.) Brenan a través de un panel de actividades biológicas. El extracto hidroalcohólico de A. colubrina se obtuvo por maceración, se evaporó por rotación y se liofilizó. Las pruebas de concentración mínima inhibitoria (MIC) y concentración mínima de fungicida (CFM) se realizaron contra Candida albicans (ATCC 10231 y cepa clínica), Candida tropicalis (ATCC 750) y Candida krusei (ATCC 6258) mediante microdilución. La actividad antiinflamatoria se verificó mediante edema de la pata inducido. Dadas las pruebas realizadas, el extracto de A. colubrina mostró una fuerte actividad antifúngica contra C. albicans (ATCC 10231) y C. krusei (MIC: 7,81 µg / ml y 31,25 µg / ml, respectivamente). Para la cepa clínica de C. albicans y C. tropicalis, la actividad antifúngica se consideró débil (MIC: 500 µg / ml y> 2000 µg / ml, respectivamente). Para las pruebas de CFM, el extracto mostró actividad fungistática en C. albicans (ATCC 10231 y cepa clínica) y C. tropicalis (CFM:> 1000 µg / ml,> 2000 µg / ml,> 5000 µg / ml, respectivamente), observando el efecto fungicida solo en C. krusei (CFM: 31.25 µg / mL). Con respecto a la actividad antiinflamatoria, entre las concentraciones del extracto analizado, el administrado en la dosis más baja presentó una mayor reducción del edema inducido de la pata. En consecuencia, el potencial anti-Candida de A. colubrina. Es interesante realizar estudios que puedan confirmar el potencial antiinflamatorio de la planta bajo prueba

Inflammatory effect of green propolis on dental pulp in rats

Pulpotomy in deciduous teeth is a controversial issue, especially with regard to alternative materials used for the direct pulp capping of the root canal pulp tissue. The aim of the present study was to perform a histological analysis of the initial reaction of the root canal pulp tissue in rats, following pulpotomy and pulp capping with (1) green propolis extract, (2) iodoform paste, (3) green propolis extract + iodoform and (4) calcium hydroxide paste with saline solution. Analyses were performed after 24 hours, 72 hours and 7 days. The substances containing green propolis extract and iodoform led to the production of an intense inflammatory infiltrate and necrosis in the root canal pulp tissue throughout the analyses. In the calcium hydroxide group, inflammatory infiltrate only prevailed at the 72-hour evaluation. Among the substances tested, calcium hydroxide paste induced the lowest intensity of inflammatory response in the root canal pulp tissue. Longer studies should be carried out to analyze the pulp repair process following pulpotomy and pulp capping with the compounds analyzed

Avaliação in vitro do selamento marginal de sistemas adesivos autocondicionantes

Objetivo: Avaliar in vitro o selamento marginal de restaurações classe II em resina composta após a aplicação de sistemas adesivos autocondicionantes. Metodologia: A amostra foi composta por quarenta terceiros molares humanos hígidos, nos quais foram realizados preparos cavitários Classe II nas faces mesial e distal, sendo os elementos dentários divididos em quatro grupos (n=20) de acordo com o tipo de adesivo utilizado: G1: Clearfil SE Bond (Kuraray), G2: Adper SE Plus (3M/ESPE), G3: AdperEasyOne SE (3M/ESPE), G4: GO! (SDI). Todas as cavidades foram restauradas com resina composta Filtek Z250 (3M/ ESPE). Após armazenamento, os elementos foram submetidos à termociclagem (500 ciclos: 5°C/55°C) e imersos em solução de azul de metileno a 2% por 24 horas.Os espécimes sofreram corte no sentido longitudinal e foi realizada a avaliação do grau de microinfiltração na interface dente/material restaurador através de escores. Os resultados foram avaliados com o auxílio do software SPSS (Statistical Package for the Social Sciences) na versão 15 por meio do teste de Kruskal-Wallis, sendo adotando um nível de significância de 5%. Resultados: Em relação aos substratos dentina ou esmalte, não houve diferença estatística significativa quanto aos diferentes tipos de adesivos utilizados (p>0,05). Entretanto, entre os diferentes grupos comparados para o substrato esmalte, observou-se diferença significativa entre os sistemas adesivos Clearfil SE Bond e Adper SE Plus (

Anadenanthera Colubrina vell Brenan: anti-Candida and antibiofilm activities, toxicity and therapeutical action

Abstract: We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis

Antimicrobial and Antiproliferative Potential of Anadenanthera colubrina (Vell.) Brenan

The aim of the present study was to perform an in vitro analysis of the antimicrobial and antiproliferative potential of an extract from Anadenanthera colubrina (Vell.) Brenan (angico) and chemically characterize the crude extract. Antimicrobial action was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration, and the inhibition of formation to oral biofilm. Cell morphology was determined through scanning electron microscopy (SEM). Six strains of tumor cells were used for the determination of antiproliferative potential. The extract demonstrated strong antifungal activity against Candida albicans ATCC 18804 (MIC=0.031 mg/mL), with similar activity found regarding the ethyl acetate fraction. The extract and active fraction also demonstrated the capacity to inhibit the formation of Candida albicans to oral biofilm after 48 hours, with median values equal to or greater than the control group, but the difference did not achieve statistical significance (P>0.05). SEM revealed alterations in the cell morphology of the yeast. Regarding antiproliferative activity, the extract demonstrated cytostatic potential in all strains tested. The present findings suggest strong antifungal potential for Anadenanthera colubrina (Vell.) Brenan as well as a tendency toward diminishing the growth of human tumor cells